The invention provides a biochemical joint preparation method and application of absolute
rose oil. The preparation method comprises the steps that coarse rose
powder is prefermented by beta-
glucosidase,
amylase and
cellulase to generate
aroma,
reflux extraction is conducted for 3-5 h with an
aqueous solution the pH value is regulated to be 9-14, a
Maillard reaction is conducted for 3-5 h, suction
filtration is conducted, filtrate is retained, an
ethanol solution is added into filter residues for secondary
reflux extraction, suction
filtration is conducted, filtrate is retained, the filtrates obtained in two times of suction
filtration are mixed, concentration and
alcohol precipitation are sequentially conducted, and a
rose extract is obtained; the
rose extract is purified through molecular
distillation, light components are retained, heavy components are purified through molecular
distillation again, the light components are mixed, and the purified absolute
rose oil is obtained. The absolute
rose oil has purer and richer
aroma and fewer impurities, and the
irritation is reduced. When the absolute rose oil is applied to reconstituted tobaccos, the color and luster of the reconstituted tobaccos is more natural and uniform, the
aroma can be enriched,
irritation and offensive
odor can be reduced, and the sensory quality can be improved; when the absolute rose oil is applied to a novel
tobacco product atomizing agent, the aroma is enriched, the noble rose note is improved, and the tobacco aroma is increased.