The invention relates to a Donkey-hide gelatin essence hydrating mask and a preparation method thereof. The mask is prepared from the following raw materials: Donkey-hide gelatin essence, a cucumber extract, a pomegranate extract, a seaweed extract, a palmetto extracting solution, dipotassium glycyrrhizinate, lubrajel oil, glycerinum, 1,3-butanediol, allantoin, sodium hyaluronate, carbomer and triethanolamine; Donkey-hide gelatin essence contains 4% of free amino acid in mass fraction, 80 to 85% of small peptide in mass fraction, and 14% of peptide fragment in mass fraction, wherein the molecular weight of small peptide ranges from 200 to 1,000Da; the molecular weight of peptide fragment ranges from 1,000 to 3,000Da; the pH (Potential of Hydrogen) of the hydrating mask ranges from 5.0 to 7.5. The Donkey-hide gelatin essence hydrating mask has the effects of hydrating, removing wrinkles, increasing skin elasticity, and enabling exquisite, smooth and glossy skin; the anti-allergic test shows that the Donkey-hide gelatin essence hydrating mask has no side effects, is safe and reliable and can be used for a long time as a mask product for skin care.