The invention discloses polypeptide anti-wrinkle firming eye cream and a preparation process thereof. The polypeptide anti-wrinkle firming eye cream comprises the following components in percentage bymass: a material A, a material B, a material C, a material D and 0-1% of polypeptide, wherein the material A comprises, in percentage by mass, 40-50% of deionized water, 4-8% of butanediol, 3-6% of glycerol, 0-0.1% of p-hydroxyacetophenone, 0-0.5% of sodium polyacrylate and 0-0.5% of EDTA disodium salt; the material B comprises, in percentage by mass, 5-10% of squalane, 2-4% of docosyl alcohol, 2-4% of polyglycerol-3 methyl glucose distearate, 2-4% of polydimethylsiloxane, 1-3% of diisostearyl malate, 1-3% of meadowfoam seed oil, 0-1% of stearic acid, 0-1% of an emulsifier and 0.5%-3.5% of emollient; and the material C comprises, in percentage by mass, 1-3% of phytosterol / behenyl alcohol / octyldecyl lauroyl glutamate, 1-3% of 1,2-pentanediol and 0-1% of a thickening agent. The polypeptideraw material is added into the eye cream, so wrinkles can be effectively removed and resisted, and better wrinkle removing and wrinkle resisting effects are obtained after continuous use of the eye cream.