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Preparation method and medicinal purpose of Larix olgensis extractive

A technology of Larix japonica and its extracts is applied in the directions of food preparation, pharmaceutical formulations, preparations for skin care, etc., to achieve the effect of inhibiting tyrosinase activity and improving repairing effect.

Active Publication Date: 2014-07-30
郑毅男 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above research reports are all studies conducted on samples of Xing’an larch extract or pure dihydroquercetin, and the biological activity research of Changbai larch extract has not been reported yet.

Method used

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  • Preparation method and medicinal purpose of Larix olgensis extractive
  • Preparation method and medicinal purpose of Larix olgensis extractive
  • Preparation method and medicinal purpose of Larix olgensis extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Raw material processing: take the root of artificially planted Changbai larch, first decompose and organize, wash the sediment with clean water, air-dry, remove bark and hair roots, rotary cut into slices, and then crush into wood chips (0.4×0.4×8mm);

[0038] 2) Extraction: material: hemicellulase: purified water (pH=5): ethanol (95%)=400:0.2:960:1440, the above is the weight ratio; the specific process is as follows:

[0039] a Enzymolysis: 400kg of material into 3m 3 In the heat reflux extraction tank, add the prepared enzyme solution into the purified water and mix evenly into the extraction tank, heat to 55-60°C for 1.5-2h; b) After the enzymatic hydrolysis process is completed, add 1440kg of ethanol (95% ), heated to 85°C, kept warm for 1 hour, and then opened the outlet valve of the extraction tank, so that 800L of the extract was concentrated in a single-effect evaporator under vacuum (-0.08MPa). The solvent is pumped into the extraction tank. After repeatin...

Embodiment 2

[0047] 1) Raw material processing: take the root of artificially planted Changbai larch, first decompose and organize, wash the sediment with clean water, air-dry, remove bark and hair roots, rotary cut into slices, and then crush into wood chips (0.4×0.4×8mm);

[0048] 2) Extraction: material: hemicellulase: purified water (pH=6): ethanol (95%)=400:0.2:960:1440, the above is the weight ratio; the specific process is as follows:

[0049] a Enzymolysis: 400kg of material into 3m 3In the heat reflux extraction tank, add the prepared enzyme solution into the purified water and mix evenly into the extraction tank, heat to 55-60°C for 1.5-2h; b) After the enzymatic hydrolysis process is completed, add 1440kg of ethanol (95% ), heated to 85°C, kept warm for 1 hour, and then opened the outlet valve of the extraction tank, so that 800L of the extract was concentrated in a single-effect evaporator under vacuum (-0.08MPa). The solvent is pumped into the extraction tank, and after repea...

Embodiment 3

[0057] 1) Raw material processing: take the root of artificially planted Changbai larch, first decompose and organize, wash the sediment with clean water, air-dry, remove bark and hair roots, rotary cut into slices, and then crush into wood chips (0.4×0.4×8mm);

[0058] 2) Extraction: material: hemicellulase: purified water (pH=5): ethanol (95%)=400:0.2:960:1440, the above is the weight ratio; the specific process is as follows:

[0059] a Enzymolysis: 400kg of material into 3m 3 In the heat reflux extraction tank, add the prepared enzyme solution into the purified water and mix evenly into the extraction tank, heat to 55-60°C for 1.5-2h; b) After the enzymatic hydrolysis process is completed, add 1440kg of ethanol (95% ), heated to 80°C, kept warm for 1 hour, and then opened the outlet valve of the extraction tank, so that 800L of the extract was concentrated in a single-effect evaporator under vacuum (-0.08MPa). The solvent is pumped into the extraction tank, and after repe...

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Abstract

The invention provides a method to extract dihydroquercetin from roots of a particular tree species Larix olgensis. The method includes the steps of selection of raw materials, enzyme treatment, extraction, concentration, hydrolysis, extraction, recrystallization, and spray drying. Composition of the flavonoid compounds in the extractive of Larix olgensis is confirmed by HPLC-MS, and the extractive mainly comprises dihydroquercetin, and others compounds of aromadendrin, eriodictyol, quercetin and kaempferol. In vitro experiments prove that dihydroquercetin extractive form Larix olgensis has strong inhibitory effects on tyrosinase activity, and the effect of scavenging DPPH free radicals. Animal in vivo experiment demonstrates that the Larix olgensis extractive has good recovery effect on carbon tetrachloride induced liver injury.

Description

technical field [0001] The invention relates to a preparation method and medical application of an extract of long white larch. The extract contains dihydroquercetin as a main component and has good medical and health care applications. Background technique [0002] Larix is ​​the main tree species in the forests of Northeast my country, Inner Mongolia, North China and Southwest alpine coniferous forest regions. There are 10 species of larch in my country, which are widely distributed in Jilin, Heilongjiang, Liaoning, Shanxi, Shaanxi, Hebei, Gansu, Xinjiang, Sichuan, Yunnan, Tibet and other provinces and regions. Larix is ​​one of the three major coniferous timber species in Northeast China . Larix is ​​a tree species that grows in cold temperate and temperate zones. It is the most cold-resistant among conifer species, and its vertical distribution can reach the upper limit of forest distribution. The natural distribution and artificial cultivation of larch in northern Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40A61K31/352A61K8/49A61K8/97A61P39/06A61P1/16A61Q19/02A61Q19/08A61Q19/10C11D9/26C11D9/02C11D9/60A23L1/30
CPCA61K8/498A61K8/97A61Q19/02A61Q19/08A61Q19/10C07D311/32C07D311/40C11D9/02C11D9/26C11D9/38
Inventor 沈月王程宇刘宇付警辉柴婧张卫鹏郑毅男沈立乾
Owner 郑毅男
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