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49results about How to "Positive and obvious technical effect" patented technology

Bedstead-type factory-like toadstool cultivation method

The invention provides a bedstead-type factory-like toadstool cultivation method. The bedstead-type factory-like toadstool cultivation method includes the steps of selection of toadstool strains, preparation of nutrition bags, selection and treatment of culture media, construction of a cultivation bedstead, seeding and earthing, mycelial culture management and fruiting management. The bedstead-type factory-like toadstool cultivation method has the advantages that the problems of seasonal limitation in toadstool production, unstable safety and yield of cultivated food and the like are solved; mycelial culture and fruiting management are carried out in a same breeding chamber, and accordingly, complexity and cost of production are reduced; pretreated soil media are used as main culture media, an annualized cultivation technique is realized completely through factory-like mushroom house conditions, limitation in climate and season cultivation is broken through, three-dimensional annualized production, high-efficiency ecology and safety in fruiting are achieved, and safe, fresh and delicious toadstool products can be obtained all year round.
Owner:SHANGHAI ACAD OF AGRI SCI

Liquid baking powder blusher and preparation method thereof

The invention provides a liquid baking powder blusher. The liquid baking powder blusher is prepared from the following raw materials in percentage by mass: 4-25% of a filling agent, 1-15% of a skin feeling regulator, 1-20% of a coloring agent, 1-50% of pearl powder, 12-30% of an emollient, 2-8% of an excipient and 0.2-3% of a preservative. The invention further provides a preparation method of theliquid baked powder blusher. Compared with a common baked powder blusher, the liquid baking powder blusher is better in powder block coloring property and more moist in skin feeling; and meanwhile, due to the liquid physical characteristics of low viscosity and high fluidity of the liquid baking powder blusher at a high temperature, a baked, cooled and formed powder block has a finer and more three-dimensional appearance.
Owner:丽鑫生技化妆品(上海)有限公司

Artificial intelligence assisted prostate tumor early diagnosis method based on surface enhanced Raman spectroscopy

The invention discloses an artificial intelligence assisted prostate tumor early diagnosis method based on surface enhanced Raman spectroscopy. The method comprises the following steps of: collectinga serum sample; adopting a Raman spectrometer for detection; performing graphic analysis and data processing on an acquired Raman spectrum signal; selecting a biological tissue Raman spectrum concentration range of 400-1800cm<-1> for analysis, and preprocessing an original spectrum acquired by a spectrograph through Origin Pro 8 software, i.e. performing background reduction and spectrum area homogenization, drawing a serum average Raman spectrum, and then comparing changes and differences of related Raman characteristic peaks, thus finally obtaining a diagnosis result of prostate tumor earlydiagnosis. The method provided by the invention is higher in sensitivity and specificity, and unnecessary needle biopsy and missed diagnosis caused by an existing screening diagnosis method are reduced.
Owner:RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Morchella esculenta cultivation method

A morchella esculenta cultivation method includes steps: strain selection; sowing pretreatment; sowing, to be specific, sowing morchella esculenta in a facility greenhouse at a temperature below 20 DEG C in November every year; nutrient bag placement; fruiting management, to be specific, regulating soil and air humidity in fruiting to keep soil humidity in a range of 65%-75% and air humidity in arange of 85%-95%; morchella esculenta harvesting. By morchella esculenta interplanting in the facility peach orchard greenhouse, the idle land in the greenhouse is used completely, the land utilization rate of the facility peach orchard greenhouse is increased by 20%-30%, a morchella esculenta cultivation environment is evidently improved, morchella esculenta yield and quality are guaranteed, andeconomic benefits of the peach orchard greenhouse are increased. In addition, soil in the facility peach orchard greenhouse is improved, soil fertility is improved, fertilizer consumption is reduced,and yield increasing and income increasing are realized.
Owner:SHANGHAI ACAD OF AGRI SCI

Method for preparing high-adsorption ceramsite by using biochar

The invention discloses a method for preparing high-adsorption ceramsite by using biochar. The method comprises: drying biomass waste, crushing, carrying out oxygen limited pyrolysis for 1-5 h at a temperature of 200-600 DEG C, cooling to a room temperature to obtain biochar, completely mixing biochar and any one or more than two selected from biogas residue powder, kaolin powder, illite, chlorite, dolomite powder, biogas residue powder, montmorillonite, bentonite powder, diatomite powder and perlite powder, stirring, granulating, roasting for 0.1-3 h at a temperature of 500-1200 DEG C, cooling, breaking, and screening to obtain the high adsorption ceramsite. According to the present invention, the high-adsorption porous biochar ceramsite with stable chemical properties can be obtained bycarrying out mixed sintering on biochar, kaolin and the like; and with the method, the pore structure of ordinary ceramsite is optimized, the ion exchange capacity is improved, the adsorption of conventional ceramsite is enhanced, and the obtained product can be efficiently used in the fields of sewage treatment, soil improvement, environmental restoration and the like.
Owner:SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING

Laparoscope lens antifogging method and device

PendingCN108992030APlay anti-fog effectSolve the problem of heatingSurgeryEndoscopesAbdominal cavityWater vapor
The invention relates to a laparoscope lens antifogging method, includes a process of heating a laparoscope lens body, repeatedly rubs an outer surface of the laparoscope lens body with a thermochromic rubber pad, heats the laparoscope lens body through utilizing heat generated through friction, on the basis of the heat transfer principle, further heats the lens of a laparoscope and achieves the antifogging effect of the laparoscope lens. The invention further provides a laparoscope lens antifogging device, the laparoscope lens antifogging device comprises the laparoscope, wherein the laparoscope comprises the lens, a body and a handle, the lens is arranged at a front end of the body, the body and the handle are connected, an outer side of the body is provided with a rubber pad, the rubberpad is distributed with thermochromic additive, one side of the rubber pad is provided with an arc-shaped groove, and the arc-shaped groove is matched with the outer side of the body. The laparoscopelens antifogging method and device are advantaged in that the frictional heat generation principle is utilized, the laparoscope lens is heated to the temperature to prevent water vapor in the abdominal cavity from condensing due to coldness before the lens, and thereby the antifogging effect of the lens is achieved, and the laparoscope lens antifogging method and device are simple, fast, economical and practical.
Owner:RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Flexible epoxy resin and preparation method thereof

The invention provides a flexible epoxy resin of which the structural formula is disclosed in the specification. The invention also provides a preparation method of the flexible epoxy resin, which comprises the following steps: weighing cardanol glycidol ether and dichloromethane, stirring uniformly while adding phosphoric acid and acetic acid, reacting at room temperature for 20-60 minutes, adding oxydol, reacting for 5-10 hours while controlling the temperature at 30 DEG C or below, washing with water to a neutral state, extracting, washing with deionized water, and removing the water to obtain the product. The cured resin has favorable toughness, thereby overcoming the defect of high brittleness of the cured bisphenol A epoxy resin.
Owner:上海美东生物材料股份有限公司

Application of protein DEPDC1 (Dishevelled and EGL-10, Pleckstrin Domain-Containing protein 1) as marker for diagnosing triple negative breast cancer

The invention provides application of protein DEPDC1 (Dishevelled and EGL-10, Pleckstrin Domain-Containing protein 1) as a marker for diagnosing the triple negative breast cancer, and also provides application of miR-26b for preparing a medicine for treating the triple negative breast cancer. The invention also provides application of miR-26b for preparing a medicine for inhibiting DEPDC1 mRNA (Ribonucleic Acid) expression. The invention finds that DEPDC1 expression is obviously higher than a paired para-carcinoma tissue in a triple negative breast cancer tissue. In the triple negative breastcancer cell, overexpression DEPDC1 can accelerate cell growth and tumor formation through FOXM1 (Forkhead box protein M1) expression up-regulation. On the contrary, after the DEPDC1 is knocked down,an inverse function is displayed. In addition, in the triple negative breast cancer, miR-26b serves as a tumor inhibiting factor to directly inhibit DEPDC1 expression and weaken the acceleration function of the DEPDC1 for cell growth and clone formation. The invention points out a new important mechanism for the occurrence, the development, the regulation and the control of the triple negative breast cancer.
Owner:RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Polyester resin for two-piece can printing ink, two-piece can printing ink and preparation method thereof

InactiveCN111205443AGood viscoelastic thixotropyGood pigment wettingInksPolymer sciencePtru catalyst
The invention provides polyester resin for two-piece can printing ink. The polyester resin is prepared from the following raw materials in parts by weight: 25-32 parts of dicarboxylic acid, 12-16 parts of saturated fatty acid, 6-12 parts of dihydric alcohol, 24-32 parts of polyhydric alcohol, 8-13 parts of anhydride, 1-3 parts of lactone, 0.02-0.03 parts of a catalyst and 0.2-0.5 parts of an antioxidant. The invention further provides a preparation method of the polyester resin for the two-piece can printing ink. The invention further provides the two-piece can printing ink. The two-piece canprinting ink is prepared from the polyester resin for the two-piece can printing ink, amino resin, an organic solvent, a catalyst, organic pigment powder, filler and auxiliaries. Meanwhile, the invention further discloses a method for preparing the two-piece can printing ink from the polyester resin. The two-piece can printing ink prepared by the invention has the advantages of better viscoelasticthixotropy, pigment wettability, scratch resistance, ink flying resistance and the like, particularly, the pencil hardness can reach 7H, and the ink flying test reaches level 5.
Owner:上海超彩油墨有限公司

Method for tumor marker quantitative determination through metal-wrappage -antibody composite nano particle based on raman enhancement

The invention discloses a method for tumor marker quantitative determination through a metal-wrappage -antibody composite nano particle based on raman enhancement. The method comprises the following steps of: dispersing a metal nano particle included in an inorganic substance or an organic substance or a polymer or a precious metal into a buffer solution and adding an antibody to realize reaction,enabling the antibody to wrap the metal nano particle, thereby obtaining the metal-wrappage -antibody composite nano particle, and dispersing the metal-wrappage -antibody composite nano particle in the buffer solution after washing; and repeatedly preparing various metal-wrappage -antibody composite nano particle solution containing different antibodies and having different concentrations, and uniformly mixing the solution; mixing purified liquid containing a tumor marker with the nano particle mixed solution, after reaction, obtaining a new metal-wrappage -antibody-antigen composite nano particle formed after specific binding of the antibody and the antigen, then, performing transmission spectrum detection, and obtaining concentration of various tumor markers. The method provided by theinvention has the advantages of being simple to operate and highly sensitive and having wide detection intervals and strong specificity. The method can simultaneously perform quantitative analysis forvarious tumor markers, and has great significance in early diagnosis of tumor.
Owner:上海镭立激光科技有限公司

Preparation method of proton exchange membrane applied to proton exchange membrane fuel cell

InactiveCN109768308AIncrease hydrophilic water retention capacityImprove conductivityFinal product manufactureFuel cellsMetal-organic frameworkWater content
The invention discloses a preparation method of a proton exchange membrane applied to a proton exchange membrane fuel cell. According to the preparation method, polyvinylidene fluoride is dissolved inan N-methyl pyrrolidone solvent to prepare a glue solution; ZIFs is added into the prepared glue solution, and then uniform stirring is performed to obtain a ZIFs / PVDF / NMP mixed glue solution, wherein the mass ratio of the mixture of polyvinylidene fluoride and N-methyl pyrrolidone to ZIFs is 1:0.004-0.02; and the obtained mixed glue solution is placed horizontally to flow flat and form a membrane, and then the obtained membrane is placed in deionized water to obtain the proton exchange membrane. Through the preparation method, the metal organic framework material ZIFs is used as a proton conducting component of the proton exchange membrane, and an acid catalyzed polymerization method is adopted to prepare the proton exchange membrane material with high conductivity; and the method is simple in preparation and low in cost, the dependency on temperature and water content is greatly lowered, and the method has quite high applicability.
Owner:上海杰好生物科技有限公司

Preparation method of organosilicon surfactant used for preparing polyurethane rigid foam

The invention provides a preparation method of an organosilicon surfactant used for preparing polyurethane rigid foam. According to the preparation method, a monohydroxyl polyether taking a monohydric alcohol or an alkyl alcohol ether as an initiator is reacted with allyl chloride or methyl allyl chloride so as to obtain allyl polyoxyethylene ether or polyoxyethylene ether; by weight, the allyl polyoxyethylene ether or polyoxyethylene ether, and a low hydrogen containing polysiloxane are weighted at a weight ratio of 300-400:100-200; the allyl polyoxyethylene ether or polyoxyethylene ether is introduced into a container, then the low hydrogen containing polysiloxane is added, stirring is carried out, heating to 75 to 85 DEG C is carried out, a catalyst is added, and copolymerization reaction is carried out so as to obtain the organosilicon copolymer surfactant. The organosilicon copolymer surfactant is capable of realizing self-emulsifying in mixing with water, the appearance is transparent, and the organosilicon copolymer surfactant is capable of improving the skin of polyurethane rigid foam.
Owner:SHANGHAI MAIPU NEW MATERIAL TECH CO LTD

Aberration-eliminating aspheric three-lens operation LED head lamp

The invention belongs to the technical field of optical devices, and particularly relates to an aberration-eliminating aspheric three-lens operation LED head lamp. The aberration-eliminating asphericthree-lens operation LED head lamp comprises an LED light source A and an aberration-eliminating imaging optical lens group B. The imaging optical lens group B comprises a first lens B1, a second lensB2 and a third lens B3. The lens B1 is a spherical lens and is made of a material BK7; the lens B2 is an aspheric lens and is made of a material PMMA; the lens B3 is a spherical lens and is made of amaterial PMMA. Light emitted by the LED lamp sheet sequentially passes through the spherical lens B1, the aspheric lens B2 and the spherical lens B3 from front to back. The optical lens system of thehead lamp not only guarantees the basic uniformity of light spots, but also enables the image edges of the light spots to be clear through an efficient aberration correction method, and the interference of halo on a field of view is reduced.
Owner:FUDAN UNIV +1

Desilting system suitable for riverway

The invention provides a desilting system suitable for a riverway. The desilting system comprises at least two weir bodies, floating filter strips and ecological floating islands are arranged on the lower course of the weir bodies, the floating filter strips are installed on the lower portions of the ecological floating islands, aquatic plants are arranged in the ecological floating islands, a desilting basin is arranged on the lower course of the filter strips, the bottom of the desilting basin is lower than the bottom surface of a river bed, and an ecology landscape weir is arranged on the lower course of the desilting basin. The multi-stage stepped weir bodies can reduce the flow speed of the bottom layer of the riverway, and sedimentation of silt, particulate matter and other large suspended solids in water is facilitated. The floating filter strips can intercept and adsorb suspended solids in river water. The desilting basin plays a role of a secondary sedimentation tank, the suspended solids in the water are further precipitated. The ecology landscape weir is the end point of the whole desilting system, inflow water overflows to the lower course from the upper ends of the weir bodies, it is ensured that the final outflow water is clear, and the desilting system has a landscape efficacy.
Owner:SHANGHAI TAIHE WATER ENVIRONMENTAL TECH DEV CO LTD

Tumor microenvironment response type gene nano-micelle as well as preparation method and application thereof

The invention provides a tumor microenvironment response type gene nano-micelle. The tumor microenvironment response type gene nano-micelle comprises polyethyleneimine-phenylboronic acid and polyethyleneimine-maleic anhydride-target radical, wherein the mass ratio of the polyethyleneimine-phenylboronic acid to the polyethyleneimine-maleic anhydride-target radical ranges from (1 to 1) to (1 to 2).The invention further provides a preparation method of the tumor microenvironment response type gene nano-micelle. The invention further provides application of the tumor microenvironment response type gene nano-micelle to preparation of a medicine for treating cancers and application of the tumor microenvironment response type gene nano-micelle serving as a medicine carrier. The nano-micelle provided by the invention can efficiently load and transmit RNA (Ribonucleic Acid) and a medicine with a microenvironment response type gene enters a tumor cell target to play a role; the tumor microenvironment response type gene nano-micelle is especially suitable for loading the RNA for carrying out gene therapy on malignant tumor. The preparation method is simple and convenient and is suitable forlarge-scale production.
Owner:SHANGHAI INST OF ONCOLOGY

Method for preparing alkynyl polyether modified organic silicon surfactant by adopting copper carbene catalyst

ActiveCN112250872AAchieve synthesisAddition reaction conditions are mildTransportation and packagingMixingPolymer sciencePtru catalyst
The invention belongs to the field of chemical engineering, and provides a method for preparing an alkynyl polyether modified organic silicon surfactant by using a copper carbene catalyst, which is used for efficiently preparing the alkynyl polyether modified organic silicon surfactant by reacting alkynyl polyether with organic silicon under the action of the copper carbene catalyst. Compared witha preparation method adopting allyl polyether and alkynediol polyether, alkynyl polyether has higher activity, the addition reaction condition of alkynyl polyether and an organic silicone hydrogen compound is milder, the equipment requirement is lower, and the prepared organic silicone surfactant is applied to hard polyurethane foam, and the surfactant has more excellent physical properties.
Owner:SHANGHAI MAIPU NEW MATERIAL TECH CO LTD

Moistening moisturizing lipstick

The invention provides a moistening moisturizing lipstick. The moistening moisturizing lipstick is prepared from water, glycerine, polyethylene, paraflint, sunflower seed wax, hydrogenated polyisobutylene, pigment, pearl powder, silica, ethylhexylglycerin, caprylyl glycol, polydimethylsiloxane and PEG-10 polydimethylsiloxane. The moistening moisturizing lipstick disclosed by the invention reservesbasic characteristics of nourishing and coloring of a lipstick, besides, through an emulsifying technique, about 10% of water phase moisturizing components are prepared into a stable water-in-oil emulsifying system, water phase components are stabilized in the system but are not demulsified to separate, besides, due hardness of the lipstick can be reserved, and the lipstick can be shaped. When the moistening moisturizing lipstick is used for making-up, unique moisturizing moistening sense and cool and refreshing sense can be caused.
Owner:丽鑫生技化妆品(上海)有限公司

Degradable soil nutrition ventilating column and preparation method thereof

The invention provides a degradable soil nutrition ventilating column. The degradable soil nutrition ventilating column comprises a wet garbage compost product, active greening plant wastes, turf, perlite, a binder and a water retaining agent; the wet garbage compost product is a secondary compost fermentation product of wet garbage, and the active greening plant wastes are a product of earthwormedible greening plant wastes; the volume ratio of the wet garbage compost product, the active greening plant wastes, the turf and the perlite is 5: 2: 2: 1; in a mixture of the wet garbage compost product, the active greening plant wastes, the turf and the perlite per cubic meter, the adding amount of the binder is 1000-1200 g, and the adding amount of the water-retaining agent is 800-1000 g. Thesoil can be improved, nutrients are promoted, furthermore, the effect of environmental protection is achieved, and the degradable soil nutrition ventilating column is simple to operate and simple in structure.
Owner:SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING

Edible lipstick

The invention provides an edible lipstick. The edible lipstick is prepared from the following raw materials in percentages by mass: 10-15% of candelilla wax, 2-7% of copernicia cerifera wax, 20-34% ofsunflower seed oil, 35-50% of castor seed oil, 3-7% of hydrogenated polyisobutene, 3-5% of lauroyl lysine and 3-15% of food-grade pigment. Components of the edible lipstick are natural components, and therefore, the edible lipstick is suitable for common people; and the edible lipstick a food-grade lipstick, so it doesn't matter if people eat it by mistake, and the edible lipstick can also be used for pregnant women and children. Compared with the prior art, the edible lipstick has positive and obvious technical effects. While having the advantages of maintaining lip color, moisturizing, moistening skin and the like, the edible lipstick is non-toxic and harmless to human bodies owing to use of the food-grade raw materials.
Owner:丽鑫生技化妆品(上海)有限公司

Milk essence and preparation method thereof

InactiveCN109463708AIncreased realism of pure milk flavorIncrease natural feelingFood scienceAlcoholCapric Acid
The invention relates to milk essence. The milk essence consists of 2-methyl-3-mercaptofuran, ethyl maltol, maltol, butyric acid, 5-decanolide, delta-dodecalactone, dimethyl sulfide, furaneol, hexylicacid, sulfurol, caprylic acid, oleic acid, 2-methyl-3-furanthio, capric acid, dodecanoic acid, tetradecanoic acid, hexadecanoic acid, alcohol and propylene glycol. The invention further provides a preparation method of the milk essence. The preparation method comprises the following steps of weighing reaction raw materials in percentage by mass; and heating the raw materials in a sandwich pot, and performing stirring to a complete uniform dissolved state, so as to obtain the milk essence. The essence is added, so that pure milk taste fidelity, natural feeling and characteristic fragrance of foods are notably improved.
Owner:恩格乐香精香料(上海)有限公司

Method for repairing copper and zinc compound contaminated soil in urban relocation land by using woody plants

The invention provides a method for repairing copper and zinc compound contaminated soil in urban relocation land by using woody plants. One or two or more of poplar, camphor trees, Magnolia grandiflora, Magnolia denudata, ligustrum lucidum Ait, Broussonetia papyrifera, Lagerstroemia indica, Prunus Cerasifera, pomegranate trees, hackberry, mulberry trees, plane trees, Zelkova schneideriana, papayatrees, Cerasus yedoensis, Chinese juniper, cedar and elm are adopted, and are planted in the copper and zinc compound contaminated soil. The invention also provides a fertilizer. Pruned branches andleaves or natural fallen leaves of the trees planted in the copper and zinc compound contaminated soil are collected, air-dried and burned, ash obtained after the burning is dissolved in water, the obtained solution is filtered, and the filtered solution is used as the liquid fertilizer after being diluted. The plants are planted in the copper and zinc combined contaminated soil to purify the soil. The pruned branches and leaves or natural fallen leaves of the planted and survived trees are burned and then dissolved in the water, and the obtained solution is used for supplementing inorganic nutrients in the growth and development process of the plants as the liquid fertilizer, so the method is environmentally friendly and economical.
Owner:SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING

Effluent pretreatment system for rain and sewage mixed-flow type pump station

The invention provides an effluent pretreatment system for a rain and sewage mixed-flow type pump station. The effluent pretreatment system comprises an aeration system and a biofilm formation system,wherein the biofilm formation system is arranged at the upper part of the aeration system; the aeration system comprises at least one aeration device; any one aeration device comprises a main pipe and two or more branch pipes; the branch pipes are perpendicular to the main pipe; any one main pipe is connected with an air blower; each main pipe and each branch pipe are respectively provided with two or more aeration holes; the separation distance of the aeration holes is 60 to 120 cm; main pipes and bank lines are arranged in parallel; the biofilm formation system is composed of two or more steel pipes; the bottom parts of the steel pipes are fixedly connected with river bottom; a steel wire rope is arranged between the steel pipes; steel wire ropes are perpendicular to the bank lines; a suspension film is arranged on each steel wire rope; the lower end of each suspension film is fixed through a gabion; and floating balls are arranged on suspension films. The effluent pretreatment system provided by the invention solves the technical problems of high pollution inlet load of a river and large lockage flow rate due to outfall of the rain and sewage mixed-flow type pump station.
Owner:SHANGHAI TAIHE WATER ENVIRONMENTAL TECH DEV CO LTD

Environment-friendly shock absorber oil for automobiles and preparation method of shock absorber oil

The invention provides environment-friendly shock absorber oil for automobiles. The environment-friendly shock absorber oil is prepared from components in mass percent concentration as follows: 56.5%-65% of epoxidized rapeseed oil, 26.5%-35% of synthetic hydrocarbon, 3%-4% of a pour point depressant, 0.5%-1.5% of an extreme pressure additive and 0-3% of an antioxidant. The invention further provides a preparation method of the shock absorber oil. The oxidation resistance, the lubricity and the shear stability of the shock absorber oil are greatly superior to those of domestic same-type mineral oil type shock absorber oil, the low-temperature fluidity of the shock absorber oil can be improved obviously by means of addition of the synthetic hydrocarbon and the pour point depressant, and the shock absorber oil is all-weather environment-friendly shock absorber oil with high lubricity.
Owner:上海中孚油品集团有限公司

Preparation method of immune vaccine for resisting porcine epidemic diarrhea virus variant shxx1902

The invention relates to a preparation method of an immune vaccine for resisting a porcine epidemic diarrhea virus variant shxx1902. The preparation method comprises the following steps of culturing vero cells; a virus culture step: adding the cell culture fluid of the porcine epidemic diarrhea virus variant shxx1902 into the vero cell culture fluid, carrying out dilution passage, and culturing for 72 hours for later use; an immunogen preparation step: adding 0.2% formaldehyde into the virus liquid, and inactivating the virus at 37 DEG C; then adding into a 96-well plate paved with vero cells, and culturing in a CO2 incubator; collecting a cell supernatant, fixing the cells by using cold acetone, adding the collected supernatant into a 96-well plate paved with vero cells, and continuously culturing; and removing the supernatant, fixing the cells with cold acetone, adding an alumina gel adjuvant after inactivation verification is qualified, and mixing for use. According to the virus titer of the inactivated immunogen obtained by the method disclosed by the invention, an antibody similar to a commercial vaccine level can be generated after mice are immunized.
Owner:SHANGHAI ANIMAL EPIDEMIC PREVENTION & CONTROL CENT

Application of USP4 as autoimmune liver disease biomarker

The invention discloses an application of USP4 as an autoimmune liver disease biomarker, and belongs to the field of medical detection. USP4 is applied to preparation of the autoimmune liver disease marker. The USP4 is also applied to preparation of a fluorescent quantitative detection kit for diagnosing or predicting the autoimmune liver disease, and the kit is used for detecting the expression level of the USP4 in peripheral blood mononuclear cells. Experiments prove for the first time that the expression level of USP4 in peripheral blood mononuclear cells of patients with autoimmune liver diseases is significantly increased compared with the expression level of USP4 in healthy subjects. The USP4 expression quantity in the peripheral blood provided by the invention is used as a molecular marker, so that noninvasive detection can be carried out on the autoimmune liver disease, noninvasive dynamic monitoring can be carried out, and the kit has important clinical application value.
Owner:THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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