281 results about "Antibody antigen" patented technology

A method for enhancing fluorescence of a biomolecule includes the step of associating the biomolecule having intrinsic fluorescence with a sensing surface that contains nanostructured metal. Association of the biomolecule with the nanostructured metal enhances its intrinsic fluorescence, which is detected upon exposure to electromagnetic radiation of a suitable wavelength. The sensing surface may include capture or ligand molecule which binds to the biomolecule and sequesters it in proximity to the nanostructured metal, thereby causing its fluorescent signal to be enhanced. The method can be used in label-free bioassays for detection of interacting biomolecules, such as antibody-antigen binding.

The present invention relates to a measurement method of immune colloidal gold particle fluorescence quenching in the field of detection technology. The antibody or antigen of target compound, namely tested compound is connected on the microgranule or microwell to form solid-phase carrier, the tested sample is added, and excess colloidal gold lag also is added, so that the target compound in the sample and the antibody or antigen fixed on the microgranule or microwell plate are undergone the immunological reaction to form solid-phase carrier compound of antibody antigen, namely conjugated compound and unreacted colloidal gold lag, namely free compound, then the conjugated compound and free colloidal gold tag are separated, in the free compound a fluorescence substance is added to make fluorescence signal be quenched. The signal quenching extent is related to free gold lag quantity in the system, and the quantity of free compound is related to quantity of target compound, so that it can implement quantitative detection of target compound.

The present invention discloses an antibody / antigen detection method based on mobile equipment. The method comprises: 1) determining the excitation signal parameter of a target antibody or antigen; 2) acquiring a reaction unit, wherein the reaction unit comprises an electrode pair spread on the bottom portion of the reaction unit; 3) acquiring auxiliary detecting control modules, wherein the modules comprise an excitation signal conditioning module; 4) sampling and pretreating a body fluid sample to be detected; 5) applying an excitation signal and sampling an impedance value; and 6) carrying out data analysis to obtain the detection result, wherein mobile intelliegnt equipment is used to read the impedance value stored in the auxiliary detecting control module, the read impedance value is analyized through the detecting control software arranged in the mobile intelliegnt equipment so as to qualitatively obtain the detection result, and the detection result is uploaded to the cloud terminal. The method of the present invention has advantages of easy operation, rapid detection, high detection precision, and the like.

The present invention provides a novel method called Sequential IMmunoPeroxidase Labeling and Erasing (SIMPLE) that enables the simultaneous visualization of at least five markers within a single tissue section. Utilizing the alcohol-soluble peroxidase substrate 3-amino-9-ethylcarbazole (AEC), combined with a rapid non-destructive method for antibody-antigen dissociation, the present application discloses the ability to erase the results of a single immunohistochemical stain while preserving tissue antigenicity for repeated rounds of labeling. The present invention also provides methods for visualizing multiple antigens simultaneously.

The invention particularly relates to a kit for measuring procalcitonin and a method for measuring the content of procalcitonin. The procalcitonin assay kit includes reagent R1 and reagent R2, wherein, reagent R1 is a reaction liquid mixed with various surfactants to promote antibody antigen-specific reaction; reagent R2 is a nano Latex particle buffer, the composition includes 0.1%-2% nano latex particles labeled with anti-human procalcitonin antibody. The detection sensitivity of the kit of the invention can reach 0.12ng / ml, which is higher than the analysis sensitivity of similar products. It is a high-sensitivity, good-specificity, high-accuracy, good-precision nano-latex microparticle procalcitonin assay kit. The latex particles of different particle sizes are cross-linked with polyclonal antibodies to form conjugates of various particle sizes, and the conjugates of different particle sizes are mixed in different proportions, which can broaden the linear range of the procalcitonin assay kit. Improve detection sensitivity.

The invention discloses a single-index microfluidic chip, and a production method and an application method thereof. The single-index microfluidic chip comprises a chip body. A sampling cavity, a quantitative reaction cavity and a waste liquid cavity are formed in the chip body; a first backflow prevention device and a second backflow prevention device are arranged at the front end and the rear end of the quantitative reaction cavity correspondingly; at least one of the first backflow prevention device and the second backflow prevention device is a rubber plug anti-backflow device that comprises a rubber plug, a fluid input pipe capable of raising the fluid delivery height and a fluid output pipe capable of lowering the liquid delivery height. The front end and the rear end of the quantitative reaction cavity are provided with the backflow prevention devices, and accordingly, quantification of fluids in the quantitative reaction cavity can be guaranteed; during antibody / antigen coating, antibody / antigen coating solvents can be injected into the fluid input pipe / fluid output pipe of the rubber plug anti-backflow device, and then are subjected to incubation, washing, packaging, and vacuumizing by a vacuum drying oven before being provided with a rubber plug, so that the single-index microfluidic chip is suitable for mass production. Basically, the quality of coated antibodies / antigens is not affected by the packaging time and the packaging process.