238 results about "Myoglobin" patented technology

The present invention relates to methods and compositions for symptom-based differential diagnosis, prognosis, and determination of treatment regimens in subjects. In particular, the invention relates to the use of biomarkers, either individually or in combinations with one another to rule in or out diseases of the aorta and its branches, most particularly aortic aneurysm and/or aortic dissection, and for risk stratification in such conditions. Preferred markers include one or more of creatine kinase-BB (CK-BB), creatine kinase-MB (CK-MB), acidic calponin, basic calponin, B-type natriuretic peptide (BNP), NT-proBNP, proBNP, BNP_79-108, BNP_3-108, caldesmon, caspase-3, D-dimer, soluble elastin fragments, endothelial cell-selective adhesion molecule (ESAM), fibrillin-1, heart-type fatty acid binding protein, MMP-9, myeloperoxidase, myoglobin, smooth muscle myosin, smooth muscle myosin heavy chain, TIMP-1, free cardiac troponin I, complexed cardiac troponin I, free and complexed cardiac troponin I, free cardiac troponin T, complexed cardiac troponin T, and free and complexed cardiac troponin T, and preferred assays are configured to detect these markers.

The invention discloses premix of organic-inorganic complex trace element. The premix of the complex trace element is added with the organic trace element and the inorganic trace element, wherein the organic trace element is hydroxyl copper methionine, ferrous fumarate, hydroxyl iron methionine, hydroxyl zinc methionine, hydroxyl manganese methionine, selenomethionine and chromium picolinate. The inorganic trace element is anhydrous cupric sulfate, anhydrous ferrous sulfate, anhydrous zinc sulfate, anhydrous manganese sulfate and chromium nicotinate. The invention has the beneficial effects of greatly reducing the cost under no influence on the use effects, higher absorption and utilization and replacement of high copper, significant reduction on the discharge of the trace element, alleviating the adverse environmental impact from the culture production, effective protecting vitamin from being damaged, extending the product warranty period, increasing the myoglobin level, improving the carcass quality, enabling the animal fur to be ruddy and shiny, promoting the animal growth, improving the return of feed, promoting the body immunity and the anti-stress ability and promoting the reproductive performance of breeding stock and breeding bird.

The invention provides a turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement. The turbidimetric rapid detection kit comprises a reaction-detection integrated device, and a detection kit arranged in the reaction device, wherein a reagent R1, a reagent R2 and a calibrator are contained in the detection kit; simultaneously, quantitative detection for five important myocardial infarction-related biomarkers in one sample can be realized, and the five important biomarkers include content of troponin-I, content of D-dimer, content of myoglobin, content of hypersensitive C-reactive protein (hs-CRP) and content of heart-type fatty acid binding protein (FABP); and the turbidimetric rapid detection kit has an important clinical significance in the aspects of early diagnosis and disease course monitoring for myocardial infarction, treatment monitoring for medicines, and the like. The turbidimetric rapid detection kit provided by the invention realizes the integration of the reagents and the reaction device, and is simple, rapid and accurate to operate, high in sensitivity, strong in specificity, and low in detection cost; and the turbidimetric rapid detection kit is a detection/reagent measurement integrated kit suitable for outpatient and emergency treatment/clinical detection, and wide in instrument application range.

The invention discloses a kit for chemiluminescence immunity quantitative detection of an MYO nano magnetic particle. The kit comprises an MYO calibrator, a nano magnetic particle suspension liquid coupled with streptavidin, bioti-labeled MYO antibodies, MYO abzyme conjugate, an MYO quality control product, a chemiluminescence liquid A and a chemiluminescence liquid B, a 20-time concentrated washing liquor and a reaction tube, wherein for the MYO abzyme conjugate, used enzyme adopts horse radish peroxidase with purity RZ larger than or equal to 3.0 and activity larger than or equal to 250 U/mL. Besides, the invention further discloses a preparation method of the kit. Compared with the conventional kit, the kit provided by the invention has the advantages of high sensibility and test automation, wide measurable concentration range, long useful life of a reagent, simple operation and the like.

A detection kit of multiindex protein for diagnosing cardiovascular disease is prepared as having base plate and reaction holes on it set on reaction hole plate; including 12-200 sample holes and 5-8 standard piece holes in said reaction holes as each reaction hole bottom being set with solid phase carrier; enveloping micro lattice of eight antibody as anti-CRP, anti-Myoglobin, anti-cTnI, anti-cTnT, anti-NT-proBNP, anti-CK-MB, anti-H-FABP and anti-GPBB on said carrier for accurately diagnose said disease in multiple man-share.

A simple and rugged sheathless interface for capillary electrophoresis/electrospray ionization-mass spectrometry (CE/ESI-MS) was designed using common laboratory tools and chemicals. The interface uses a small platinum (Pt) wire which is inserted into the CE capillary through a small hole near the terminus. The position of the wire inside the CE capillary and within the buffer solution is analogous to standard CE separation operations where the terminus of the CE capillary is placed inside a buffer reservoir along with a grounded platinum electrode. By combining the use of the in-capillary electrode interface with sharpening of the fused silica tip of the CE capillary outlet, a stable electrospray current was maintained for an extended period of time. The design was successfully applied to CE/ESI-MS separations and analysis of mixtures of peptides and proteins. A detection limit of approximately 4 femtomole (S/N=3) was achieved for detection of myoglobin utilizing a 75 mum-i.d. aminopropylsilane treated CE column and using a wide scan range of 550-1350 Da. The advantages of this new design include: (1) a stable CE and ESI current, (2) durability, (3) a reduced risk of sparking between the capillary tip and the inlet of the mass spectrometer, (4) lack of any dead volume, and (5) facile fabrication with common tools and chemicals.

Nitrosylation of proteins and amino acid groups enables selective regulation of protein function, and also endows the proteins and amino acids with additional smooth muscle relaxant and platelet inhibitory capabilities. Thus, the invention relates to novel compounds achieved by nitrosylation of protein thiols. Such compounds include: S-nitroso-t-PA, S-nitroso-cathepsin; S-nitroso-lipoprotein; and S-nitroso-immunoglobulin. The invention also relates to therapeutic use of S-nitroso-protein compounds for regulating protein function, cellular metabolism and effecting vasodilation, platelet inhibition, relaxation of non-vascular smooth muscle, and increasing blood oxygen transport by hemoglobin and myoglobin. The compounds are also used to deliver nitric oxide in its most bioactive form in order to achieve the effects described above, or for in vitro nitrosylation of molecules present in the body. The invention also relates to the nitrosylation of oxygen, carbon and nitrogen moieties present on proteins and amino acids, and the use thereof to achieve the above physiological effects.

The invention discloses an antibody coating process and a myohemoglobin determination reagent kit made with the same. The problem that in the prior art, bridging of various chemical substances is generally adopted to improve the antibody and microsphere coupling efficiency, and consequently an antibody microsphere preparation process is more complex and higher in cost is solved. The process includes the steps that firstly, microspheres and an activator are dissolved in a buffer solution with the pH of 6.0 respectively; secondly, carboxyl on the surfaces of the microspheres is activated with an activator to obtain activated microspheres; thirdly, antibodies are added into the activated microspheres for coupling; fourthly, a buffer solution with the pH of 8.0 is added into the activated microspheres coupled with the antibodies to continue a reaction; fifthly, after the reaction is completed, a sealing agent is added to complete sealing. The process has the advantages that the chemical coupling efficiency is greatly improved, the operation steps are simplified, and the production cost is reduced.

Belonging to the technical field of immunodetection and analysis, the invention specifically provides a myoglobin enzymatic chemiluminescence immunodetection method. The method includes the steps of: forming a solid phase-antibody-antigen-enzyme-labeled secondary antibody immune sandwich composite and detecting the immune sandwich composite by means of chemiluminescence. The invention also provides a myoglobin enzymatic chemiluminescence immunodetection kit. The detection method and the detection kit provided in the invention are suitable for detection and analysis of myoglobin, and have the advantages of large detection range, and high sensitivity and specificity.

The invention relates to a cardiac muscle control material, a preparation method therefor, a detection kit and a detection device for cardiac muscle. The cardiac muscle control material comprises quality control component and protection component, the quality control component comprises at least one of B-type natriuretic peptide, D-dimer, NT-proBNP, cardiac troponin I, myoglobin, creatine kinase isozyme and cardioid fatty acid binding protein and heart fatty acid binding protein. The protective component comprises 5 mM to 100 mM of a buffer agent, 0.5 % to 5% by weight of an excipient, 0.05 mMto 5 mM of an antioxidant, 0.05 mM to 15 mM of a protease inhibitor, 0.5% to 10% by weight of a stabilizer and 0.01% to 2% by weight of a surfactant. The cardiac muscle control material disclosed bythe invention is good in stability.

The invention discloses a cardiac troponin (cTnI), myoglobin (Myo) and creatine kinase-MB (CK-MB) three-in-one colloidal gold test strip, a kit thereof and making methods of the test strip and the kit. The test strip and the kit are made through adopting a chromatographic technique and a double antibody sandwich principle, and the human cTnI, Myo and CK-MB levels in a clinic specimen (whole blood/serum/blood plasma) can be detected through one-time operation, so the operation process is simplified.

The invention discloses a chemiluminescence quantitative detection kit for myoglobin and a preparation method and a detection method thereof, belongs to the technical field of in vitro diagnostic reagents, and solves the technical problems of low sensitivity, long reaction time and high cost in the prior art. The kit comprises the following reagents: R: a buffer solution containing streptavidin coated magnetic particles; R2: a buffer solution containing a chemiluminescent marker-labeled myoglobin monoclonal antibody; and R3: a buffer solution containing coupling marker-labeled myoglobin monoclonal antibody. According to the chemiluminescence quantitative detection kit for the myoglobin provided by the invention, the principle of a double-antibody sandwich method is adopted, an avidin-coupling marker system is used, and chemiluminiscence is carried out by adopting a chemiluminescent marker, thus the detection sensitivity of the kit is greatly improved, the reaction time is shortened, the reagent cost is reduced, and the kit can be used for the quantitative detection of myoglobin contents in serum/plasma.

The invention belongs to the field of in-vitro diagnostic reagent kits, and particularly relates to a myoglobin detection reagent kit and a method for applying the same. The myoglobin detection reagent kit comprises calibration products, reagents R1, enzyme conjugate working solution R2, magnetic bead conjugate working solution M, cleaning solution and chemiluminescent substrates. The reagents R1contain imidazole components, blood cells in whole blood can be quickly removed by the imidazole components, and accordingly the possibility that magnetic beads are swallowed by the blood cells can beeffectively prevented; the cleaning solution contains sodium lauryl sulfate components, accordingly, cleaning effects can be enhanced, and non-specific binding of the chemiluminescent substrates canbe prevented. The myoglobin detection reagent kit and the method have the advantages that finger peripheral whole blood or anticoagulant venous whole blood can be directly used as a to-be-detected sample for the myoglobin detection reagent kit, the whole blood sample can be directly detected without being pretreated, accordingly, the detection speed can be greatly increased, operation steps can besimplified, the application range of the myoglobin detection reagent kit can be expanded, and large-scale popularization and application can be facilitated.

The invention provides a myoglobin determination kit of a compound antibody. The myoglobin determination kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a reaction liquid for promoting antigen specific binding of the antibody; the reagent R2 is a buffer solution of nano latex micro-particles combined with an anti-human-myoglobin antibody; and the reagent R2 is characterized by being prepared by virtue of the following steps: coupling polystyrene nano latex micro-particles of which the diameters are 50-300nm with a myoglobin monoclonal antibody or a polyclonal antibody to obtain conjugates with different particle sizes and different antibodies; and mixing the conjugates with different particle sizes and different antibodies according to different proportions to prepare the reagent R2. The reagent R2 can be used for detecting myoglobin in human serum, and can be used for improving the detection sensitivity and linear range of the kit provided by the invention. The detection sensitivity of the kit is 0.2mu g/L, and the linear range of the kit is 0-1200mu g/L.

The invention discloses a production method and an application of an electrode modified with gold nano-cage and myoglobin. The production method comprises the following steps: taking graphite powder and an ionic liquid HPPF6 according to a mass ratio of (1.5-2.5):1, placing the graphite powder, the ionic liquid and liquid paraffin in a mortar, carrying out uniform grinding to obtain a carbon paste, filling a glass electrode tube with the carbon pate, and inserting a copper wire as a lead wire to obtain a carbon ionic liquid electrode CILE; dispensing 6-10 [mu]L of a AuNCs solution on the surface of the CILE, and naturally drying the CILE in a dark place under room temperature conditions to obtain an AuNCs/CILE electrode; dispensing 6-10 [mu]L of a 10-20 mg mL<-1> Mb solution on the surfaceof the AuNCs/CILE electrode, and naturally drying the AuNCs/CILE electrode in a dark place at room temperature to obtain a Mb/AuNCs/CILE electrode; and dispensing 4-8 [mu]L of a 0.3-0.7% Nafion ethanol solution on the surface of the Mb/AuNCs/CILE electrode, and drying the Mb/AuNCs/CILE electrode in a dark place at room temperature to obtain the Nafion/Mb/AuNCs/CILE electrode. The modified electrode has a good electrocatalytic reduction effect on trichloroacetic acid, sodium nitrite and hydrogen peroxide, has the advantages of wide linear range, low detection limit and high sensitivity, and can be well applied to the detection of the content of above three substances in an object.

The invention discloses an immunofluorescence dipstick component for quickly and quantitatively detecting protein of a plurality of types and a detection card component prepared from the same and a preparation method thereof, wherein the protein of a plurality of types comprises muscle hemoglobin/creatine kinase isoenzyme/troponin I; the dipstick component comprises a dipstick consisting of a bottom liner, a water-absorbing pad, a coated analysis membrane and a sample pad and independently packaged fluorescein mark specific antibodies; three detection lines and a quality control line are arranged on the coated analysis membrane; the detection line on the coated analysis membrane is respectively coated with an anti-myoglobin monoclonal antibody line, an anti-creatine kinase isozyme monoclonal antibody line and a troponin I monoclonal antibody line; the quality control line is coated with a rabbit IgG antibody; the fluorescein mark specific antibodies comprise the anti-myoglobin monoclonal antibody line, the anti-creatine kinase isozyme monoclonal antibody line, an troponin I monoclonal antibody and an anti-rabbit IgG antibody; and the detection card component comprises the dipstick, a card box consisting of a cover plate and a back plate and independently packaged platinum porphyrin mark specific antibodies and can synchronously detect the muscle hemoglobin/creatine kinase isoenzyme/troponin I, is simple to operate, is quick and sensitive, and has good specificity.

The invention discloses a method, system and chip test paper for parallel detection on various cardiac markers. The chip test paper is used for detecting a part or all of the cardiac markers including cTNI (cardiac troponin I), cTNT (cardiac troponin T), MYO (myoglobin), CK-MB (isoenzymeof creatine kinase containing M and B subunits), BNP (B-type natriuretic peptide), CRP (C-reactive protein) and FABP (fatty acid-binding protein); the chip test paper comprises a first membrane and a second membrane; a ligand An of each marker is arranged on the first membrane, and a ligand Bn coupled with a signal marker is absorbed on the second membrane; and a detected material forming sandwich detection together with the ligands An and Bn is added from a sampling hole, then under the acting force of percolation or other factors, the detected material moves to be combined with the ligands An and Bn respectively to form a composite array of the first membrane-the ligand An-the detected material-the ligand Bn-the signal marker, which is fixed on the first membrane, the composite array and a capture fiber membrane are assembled simultaneously, and a detection hole is reserved. The method, the system and the chip test paper provided by the invention can be used for detecting the various cardiac markers simultaneously and quantitatively; moreover, the detection sensitivity can be improved, and the detection time is saved.

The invention discloses a preservation solution of a time-resolved fluorescent microsphere labeled myoglobin antibody. The preservation solution is prepared from the following components in percentage by mass: 0.1 percent to 1.5 percent of 4-(2-hydroxyethyl)piperazine-1-erhanesulfonic acid, 1.0 percent to 3.0 percent of sodium chloride, 0.3 percent to 0.8 percent of potassium chloride, 0.08 percent to 0.9 percent of disodium ethylene diamine tetraacetate, 0.01 percent to 0.06 percent of sodium dodecyl sulfonate, 0.1 percent to 3.0 percent of bovine serum albumin, 0.05 percent to 0.5 percent of glucan, 0.02 percent to 0.2 percent of triton, 0.01 percent to 0.05 percent of Tween-20, 0.02 percent to 0.1 percent of a preservative and the balance of water; the pH (Potential of Hydrogen) is adjusted to be 8.0 to 9.0. The preservation solution has the advantages that the raw materials are low in cost and easy to obtain, and a relatively stable microenvironment is provided for preservation of fluorescent microspheres.