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Tachysynthesis fluorescent quantitation detection kit for multi-index detection of myocardial infarction

A fluorescent quantitative detection and rapid immunization technology, applied in the field of biomedicine, can solve problems such as low sensitivity and mutual interference of markers, and achieve the effect of saving diagnosis and treatment costs and waiting time.

Inactive Publication Date: 2016-06-22
苏州联辰生物技术有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide a rapid immunofluorescence quantitative detection kit for multi-index detection of myocardial infarction, aiming to solve the problems of low sensitivity and mutual interference between multiple biochemical markers in the prior art

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  • Tachysynthesis fluorescent quantitation detection kit for multi-index detection of myocardial infarction
  • Tachysynthesis fluorescent quantitation detection kit for multi-index detection of myocardial infarction
  • Tachysynthesis fluorescent quantitation detection kit for multi-index detection of myocardial infarction

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specific Embodiment approach

[0045] The present invention will be further described below in conjunction with specific examples.

[0046] The present invention is an immunofluorescence kit for the quantitative detection of troponin I, myoglobin, and CK-MB, which utilizes the high-sensitivity characteristics of fluorescence, combines different direct fluorescent particles and specific labels of corresponding antibodies, and cooperates with an optimized process, The detected substance flows smoothly over the surface of the nitrocellulose membrane, the data is obtained by the supporting fluorescence detector, and the high-sensitivity detection result is calculated. The reagent sample solution comprises surfactant, blocking agent, preservative, and labeled anti-human troponin antibody, labeled myoglobin antibody, and latex particles of labeled CK-MB antibody, and each labeled antibody in the reagent is combined with The latex particles are connected by a peptide chain system; the calibrator includes a protect...

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Abstract

The invention discloses a rapid immunofluorescence quantitative detection kit for multi-indicator detection of myocardial infarction. The rapid immunofluorescence quantitative detection kit for multi-indicator detection of myocardial infarction includes a nitrocellulose membrane, and one end of the nitrocellulose membrane is provided with The sample pad is provided with a water-absorbing pad at the other end of the nitrocellulose membrane, and the area between the water-absorbing pad and the sample pad of the nitrocellulose membrane is respectively coated with anti-troponin I, myoglobin, CK- MB antibody and internal quality control antibody. The invention utilizes the fluorescence characteristics of fluorescent particles with different diameters, combines chemical cross-linking labeling technology and immunochromatography technology, and realizes immunofluorescence quantitative detection on the basis of optimizing each component of the test strip. Compared with the conventional colloidal gold immunochromatography method, the present invention has the advantages of good labeling stability, low non-specificity, high sensitivity, small cross-interference and accurate quantification.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a rapid immunofluorescence quantitative detection kit for detecting myocardial infarction with multiple indexes. Background technique [0002] Acute myocardial infarction (AMI) is a serious threat to human health, and early diagnosis and treatment of such diseases is one of the important means to reduce patient mortality. Myocardial infarction (MI) is a disease in which coronary artery occlusion and blood flow interruption cause local necrosis of some myocardium due to severe persistent ischemia. Clinical symptoms include severe and long-lasting retrosternal pain, fever, leukocytosis, accelerated erythrocyte sedimentation rate, increased serum myocardial enzyme activity, and progressive ECG changes. Arrhythmia, shock, or heart failure may occur. Myocardial infarction is a kind of heart disease belonging to coronary atherosclerosis. Cardiac markers refer to a series of bio...

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Application Information

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IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/68G01N33/533
Inventor 翁濬一孙京海马雪林
Owner 苏州联辰生物技术有限公司
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