86 results about "Intrinsic fluorescence" patented technology

The present invention provides for a monitoring system and method to monitor an analyte, including determining the presence of the analyte and analyzing the analyte; for example, cellular chorography and biological threats. The system and method use a fluidics apparatus (e.g., a flow cytometer), a computer, a probe synthesizer and/or an initial set of fluorophore conjugated probes to monitor the analyte. The fluidics apparatus is adapted to determine the presence of the analyte based on the intrinsic fluorescence of the analyte, and/or to determine the binding of the probes to the analyte in the sample. The binding information is analyzed by the computer by comparing the binding results with a database of information regarding the analyte. The information gained allows the probe synthesizer to synthesize new probes for a subsequent iteration of the process, wherein additional information regarding the analyte is gained with every iteration.

A method and system for analysis of fluorescence emission spectroscopy data and fluorescence lifetime imaging microscopy data are described. A unique Laguerre expansion can be found for fluorescence intensity decays of arbitrary form with convergence to a correct solution faster than with conventional methods. The Laguerre expansion technique includes expansion coefficients highly correlated with intrinsic fluorescence lifetimes, allowing direct characterization of fluorescence dynamics. For complex systems, conventional analysis of fluorescence intensity decay in terms of discrete exponential components can not readily provide a true representation of underlying fluorescence dynamics. Utilizing the Laguerre expansion technique, an alternative non-parametric method for analysis of time-resolved fluorescence data from various systems is described, facilitating characterization and discrimination of a sample. An ultra-fast method for analysis of fluorescence lifetime imaging is also described, facilitating real-time analysis of compositional and functional changes in samples, at a microscopic or macroscopic level.

“A label-free imaging method to monitor stem cell metabolism discriminates different states of stem cell as they differentiate in a living tissues. We use intrinsic fluorescence biomarkers and the phasor approach to Fluorescence Lifetime Imaging Microscopy (FLIM). We identify and map intrinsic fluorophores such as collagen, retinol, retinoic acid, flavins, nicotinamide adenine dinucleotide (NADH) and porphyrin. We measure the phasor values of germ cells in C. Elegans germ line. Their metabolic fingerprint cluster according to their differentiation state, reflecting changes in FAD concentration and NADH binding during the differentiation pathway. The phasor approach to lifetime imaging provides a label-free, fit-free and sensitive method to identify different metabolic state of cells during differentiation, to sense small changes in the redox state of cells and may identify symmetric and asymmetric divisions and predict cell fate.”

In order to improve fluorescence measurements, there is provided an apparatus and, a method and a computer program for optical analysis of an associated tissue sample, the apparatus comprising a spectrometer comprising an optical detector, a light source, a first light emitter 219 arranged for emitting photons into the associated tissue sample, a first light collector 221 arranged for receiving photons from the associated tissue sample, a second light emitter 223, a second light collector 225, wherein a reflectance spectrum is obtained via the first light emitter 219 and collector 221 and a fluorescence spectrum is obtained via the second light emitter 223 and collector 225, and where a first distance d1 between the first light emitter and collector is larger than a second distance d2 between the second light emitter and collector. By combining the data thus obtained, an intrinsic fluorescence spectrum may be obtained.

An apparatus and method for improving aerosol particle characterization and detection accuracy in clean room applications that includes an optical particle sizer that receives a particle containing aerosol sample at a higher flow rate which is operatively coupled to an inertial aerosol concentrator for concentrating particles received from the optical particle sizer and delivering a lower flow rate, particle enriched output. The system further includes an optical sensor for sensing of intrinsic particle fluorescence of the lower, particle-enriched flow, since intrinsic fluorescence is a useful indicator of biological particles and biological particle viability, including bacterial particles. The system as a whole provides a measure derived from a single inlet flow both of total particles and of viable microbial particles based on their spectroscopic properties.

A optical Biopsy method and an apparatus are used in the diagnosis of precancerous lesion for locating the place and determining the level of malignant tumor. The apparatus comprises light source (1, 10) a light channel system, an endoscope (21) and a circuit system. The light sources include an excited light (1) and a cold light source (10). The cold light source and the excited light in the light channel system go through the end of the light guide of the endoscope via optical fiber bundle and irradiate the tested living tissue (22). The white light image signal and the intrinsic fluorescence image signal reflected from the tested living tissue (22) are received by a weak fluorescence CCD (6) that tightly connects to the end of the endoscope (21) and then transmit to the circuit system via a signal wire (9) to produce the image in the display (17). The weak fluorescence signal reflected from the tested living tissue (22) is transmitted to the circuit system via the weak fluorescence fiber bundle (4) protruding from the forceps hole of the endoscope to produce the spectrum image (16).

Disclosed is a system using each of the descrete emitters in a III-nitride micro-emitter array as a light source for measuring the properties of independent samples of biological materials deposed on a micro-array using some form of detecting device, e.g, a detector array or charge-coupled device. In embodiments the emitter array produces deep ultraviolet in investigating protein-protein interactions or to detect biological and chemical molecules with high specificity by monitoring changes in a protein's intrinsic fluorescence.

The present invention relates to methods of detecting specific cell surface antigens present in a sample of cells being tested and in particular blood group antigens, which methods do not employ the addition of extrinsic labels to detect said cell surface antigens. Typically detection is carried out using an intrinsic fluorescence capability of the cells being tested.

Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of intrinsic fluorescence of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.

Glass as a substrate for fluorescence-based assays is expensive. Disclosed is a poly(cyclic olefin) alternative to glass that is of comparable sensitivity. More specifically, disclosed is a method of making a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, said method comprising providing a substrate comprising a poly(cyclic olefin); protecting the substrate from ultraviolet (UV) light; and subjecting a surface of said UV-protected substrate to ozone oxidation to activate said surface; wherein a content of intrinsic fluorophores on the surface of the substrate remains substantially unchanged after the ozone oxidation. Also disclosed is a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, said microdevice comprising a body comprising a poly(cyclic olefin), said body having an ozone-activated surface substantially free of intrinsic fluorophores; wherein said activated surface is substantially free of intrinsic fluorescence during a fluorescence-based assay of said biomolecules bound to said activated surface.

Simple organic structures, organic/inorganic polymers, and other substrates have been made, all of which have at least one pyrrolidone moiety present, and found to exhibit low toxicity, low complement activation features and may be used to reduce protein interactions with drug conjugates while enhancing in vivo residency times for these conjugates when used as an injectable composition; thus these compounds can be used as substitutes for PEG in PEGylation. Surprisingly, these compounds also exhibit unique intrinsic fluorescence (IF) or non-traditional fluorescence (NTF) properties that currently cannot be explained by traditional photochemistry and fluorescence paradigms are described. These compounds have a variety of applications such as in cellular imaging, gene transfection, bio-diagnostics, biosensing, fluorescence directed surgical resections, drug delivery, forensics, environmental diagnostics, mineral/gemstone characterization, counterfeit goods detection, tracer studies related to liquid/water flow, oil field enhancements and diagnostics, prevention of photo-bleaching, and LED display enhancements and others.