487 results about "Fluorescence spectra" patented technology

The invention provides systems for automatically localizing areas of disease within a tissue sample using fluorescence spectra, reflectance spectra, and video images obtained from the sample. The invention further provides systems for displaying, marking, and treating the identified areas so that healthy surrounding tissue suffers minimal impact upon treatment and / or excision of the diseased tissue. The invention facilitates minimally-invasive treatment by accurately directing treatment to only those areas that are identified as diseased.

Measurements of fluorescence spectra of fluid samples recovered downhole are processed to give the fluid composition. The processing may include a principal component analysis followed by a clustering method or a neutral network. Alternatively the processing may include a partial least squares regression. The latter can give the analysis of a mixture of three or more fluids.

The invention provides a method used for simultaneous detection of three food-borne pathogenic bacteria based on multicolor upconversion fluorescence labeling. According to the method, three upconversion materials with differentiable fluorescence spectrums are used for forming multicolor upconversion fluorescent nanoprobes via respective connection with aptamers of staphylococcus aureus, vibrio parahaemolyticus, and salmonella, and complementary oligonucleotide single chains of the aptamers are connected with magnetic nanoparticles so as to form nano-composites. When bacteria to be tested are in a detection system, double chain unwinding is realized because of specific binding of the pathogenic bacteria with corresponding aptamers; it is possible to realize simultaneous quantitative determination of staphylococcus aureus, vibrio parahaemolyticus, and salmonella by monitoring upconversion fluorescence signal strength at 477nm, 550nm, and 660nm, detection linear range ranges from 50 to 1000000cfu / ml, and detection limits are 25cfu / ml, 10cfu / ml, and 15cfu / ml respectively. The method is used for detection of pathogenic bacteria, is high in sensitivity, is rapid and convenient, and can be used for detection of the three pathogenic bacteria in food such as milk and shrimp meat; and results are accurate and reliable.

Examples of a spectroscopy probe for performing measurements of Raman spectra, reflectance spectra and fluorescence spectra are disclosed. The integrated spectral probe can comprise one or more light sources to provide a white light illumination to generate reflectance spectra, an excitation light to generate an UV / visible fluorescence spectra and a narrow band NIR excitation to induce Raman spectra. The multiple modalities of spectral measurements can be performed within 2 seconds or less. Examples of methods of operating the integrated spectroscopy probe disclosed.

The invention discloses a laser radar system for measuring Raman spectra of atmospheric water and fluorescence spectra of aerosols.The system consists of a transmitting unit, an optical receiving and signal detection unit and a control unit.The transmitting unit adopts a seed injected solid laser to output ultraviolet laser with the narrow linewidth of 354.8 nm and guide the ultraviolet laser to the zenith.The optical receiving and signal detection unit collects backward scattering light from atmospheric substances, produces inhabitation superior to 15 orders of magnitude to nearby light of 354.8 nm and distinguishes and records signal light in the spectrum band range of 393.0-424.0 nm at the spectral precision of 0.8 nm.The control unit guarantees the orderly work of the whole radar system.Under the radiation of ultraviolet laser with the wave length of 354.8 nm, the vibrational-rotational Raman spectrum regions of gas-state, liquid-state and solid-state water sequentially correspond to the ranges of 395-409 nm, 396-410 nm and 401-418 nm.The laser radar system can simultaneously record the Raman spectra produced by the three states of water and fluorescence spectra of produced by aerosol particles and achieve simultaneous detection of the atmospheric water, aerosols and other substances.

The invention discloses an automatic detection system for the traditional-Chinese-medicine quality. Traditional-Chinese-medicine original images are collected, outlines of target areas are partitioned, the images are subjected to noise removing, a neural network PCNN corresponds to the images, central neuron corresponds to pixel points of the images, multi-wavelength LED lamps are illumed respectively, several LED lamps capable of motivating remarkable fluorescence signals are selected and alternately illumed, traditional-Chinese-medicine samples with fluorescence spectrums of 300 nm-1,100 nm are collected, the one-dimensional time-series signal characteristics of all two-dimensional images are extracted, characteristic information is stored, the airspace characteristics of traditional-Chinese-medicine microscopic images are extracted, and the traditional-Chinese-medicine quality is detected. According to the automatic detection system, the traditional-Chinese-medicine external parameters are synthetically evaluated with the electronic technology, the traditional-Chinese-medicine inherent quality is automatically detected by combing traditional-Chinese-medicine chemical-component content parameters, safety detection parameters and conventional detection parameters, the detection standard is objective and uniform, and accurate medicine making can be achieved.

The invention relates to a method for producing ultra-small water soluble near-infrared Ag2S quantum dots, which adopts the one-pot method for production. The method for producing ultra-small water soluble near-infrared Ag2S quantum dots comprises the following steps of: 1) uniformly mixing a protein water solution with an AgNO3 water solution and carrying out reaction for 5 minutes at the normal temperature under the condition of magnetic stirring; 2) adjusting the pH value of the liquid with a NaOH water solution to 12.0; and 3) adding a chalcogenide water solution with the concentration of 10-100 millimole / l into the liquid and adjusting the mole ratio of the Ag element and the S element to (6-1):1, and reacting for 12 hours under the conditions of stirring and 37 DEG C to produce the ultra-small water soluble near-infrared Ag2S quantum dots of smaller than 2 nanometers. The method has the advantages that the quantum dots produced by the method have the excellent properties of no-toxic heavy metal elements, small size, good water solubility, high luminous efficiency, adjustability in fluorescence spectra within near-inferred areas and the like; and the production method is safe, easy and convenient to operate, has small toxicity and low cost, and is easy to popularize and use in a large scale.

The invention relates to a microscopic fluorescence identification method for an arene component of a petroleum inclusion. The method comprises the following steps of: quantitatively detecting the arene component of the petroleum inclusion by a laser induced fluorescence spectrum technology under a microscopic environment; acquiring a fluorescence spectrum of fluid in the petroleum inclusion by alaser fluorescence spectrum technology under the microscopic environment; and performing correlation contrastive analysis on fluorescence spectra of a crude oil sample and an extracted oil sample to determine the maximum related oil sample of the fluid in the petroleum inclusion, wherein the arene component content of the maximum related oil sample is similar to the component content of the fluidin the petroleum inclusion. By the method, the samples are subjected to three-dimensional microarea movement, microimaging and computer imaging by microarea positioning and microimaging technologies,so that a single oil gas inclusion sample to be irradiated can be selected and positioned precisely; full fluorescence excitation and full spectrum acquisition corresponding to the arene component inthe selected single oil gas inclusion are realized by tunable laser generation and fluorescence full spectrum acquisition technologies; and the acquired fluorescence spectrum of the arene component of the single oil gas inclusion is subjected to correlation contrastive analysis by a spectrum fingerprint technology and a spectrum multiscale data analysis technology to realize oil source comparisonand determine the relative content of the arene component of the targeted inclusion.

The invention relates to a method for preparing silicon-dioxide microspheres with liposome embedded with quantum dots. The method includes the following steps: lipid material is dissolved in an organic solvent for being prepared to be a lipid film by rotary evaporation; the lipid film is added with a water solvent of the quantum dots for being prepared to be the liposome taking lipid as the film and the quantum dots as the core and with a grain size less than or equal to 10 Mum by a thin film dispersing method; the lipsome is added with tetraethyl orthosilicate to form silicon dioxide on the surface of the lipsome so as to obtain the silicon-dioxide microsphere with the liposome embedded with the quantum dots. The method has the advantages that: the silicon-dioxide microsphere with the liposome embedded with the quantum dots can decrease the toxicity of the quantum dots, increase the light signal intensity of every luminous point, and obtain microspheres with large quantity and different fuorescence spectra characteristics so as to play a part in biological high-flux detection. The quantum-dot microsphere of micron size is applicable to the cure of tumour artery embolism; and the quantum-dot silicon-dioxide microspheres with different grain sizes evenly arranging from nanometer to micron can be obtained by the method.

The invention discloses a wide-view-field chromatography hyperspectral microscopic imaging method and device based on space-time focusing, and belongs to the technical field of microscopic spectral imaging and analytical chemistry. According to the method, ultra-short pulse lasers are generated through an ultra-short pulse laser source, focusing lines are generated in a sample through space-time focusing, excited fluorescence is collected, stray light is filtered out through con-focal optical slits, spectral information (x, lambda), of the sample is acquired through fluorescence spectral information collecting, and finally sample (x, lambda, y, z, t) five-dimensional information is acquired through three-dimensional space scanning and delayed scanning. The device comprises the ultra-shortpulse laser source, a light beam conversion system, a line scanning system based on space-time focusing, an optical microscopic system and a filtering and synchronous spectrum con-focal detection system. Acquisition of the spectral information in the filtering and synchronous spectrum con-focal detection system is synchronous with line scanning trigger signals in the line scanning system in combination with the space-time focusing technology. The method and device have the advantages of being wide in view field, high in space resolution, high in time resolution, high in spectral resolution andthe like.

We report the synthesis and characterization of four novel CCC—NHC pincer platinum(II) and palladium(II) complexes, which adopt a distorted square planar configuration. These complexes emit bright blue light in the solid state under UV irradiation with emissions that are stable in ambient atmosphere (O2 and H2O) for extended periods. We also report the synthesis and characterization of CCC—NHC pincer ligand nickel complexes, and solid state fluorescence spectra have been collected for two of the complexes reported. X-ray structural analysis of a representative compound exhibits a distorted square planar geometry. Finally, we report the synthesis and characterization of CCC—NHC pincer ligand complexes for abnormal carbens, triazole, and BIA.

The invention discloses a preparation method of a Cu-Zn-In-S quantum dot luminescent thin film. The preparation method comprises the following steps: (1) adding cuprous chloride, indium chloride, zinc salt, a capping agent and a surface coating agent to a non-polar high boiling point organic solvent so as to obtain a Cu, In and Zn mixed precursor solution, stirring and heating under the atmosphere of nitrogen or inert gas so as to form a clear transparent solution; (2) adding an oleylamine solution of sulfur to the clear transparent solution obtained in the step (1), and heating for reacting so as to prepare a Cu-Zn-In-S quantum dot solution; (3) separating so as to obtain Cu-Zn-In-S quantum dots; (4) mixing the prepared Cu-Zn-In-S quantum dots with a component A of an LED (Light Emitting Diode) pouring sealant; (5) uniformly mixing a component B of the LED pouring sealant with a mixture obtained in the step (4), removing air bubbles, then coating a product on a glass substrate, and curing at a room temperature so as to obtain the Cu-Zn-In-S quantum dot luminescent thin film. The fluorescence spectra of the Cu-Zn-In-S quantum dot luminescent thin film prepared by the method can be adjusted. The Cu-Zn-In-S quantum dot luminescent thin film has the excellent fluorescence property of the Cu-Zn-In-S quantum dots and the good machining property of an organic silicon adhesive AB.

The invention relates to a copper-doped all-inorganic halogen perovskite fluorescent material and a preparation method and application thereof. The material has structural formula CsPb1-xCuxBr3, wherein X is in the range of 0-1; the material is a light yellow or yellow powder, and in cubic phase, and has a space group as below. The method is as below: dissolving in an organic solvent at high temperature for further reaction, washing with a hexane or toluene solvent by multiple times, purifying to obtain the copper-doped all-inorganic halogen perovskite fluorescent material with size of 10-30nm. By measuring of the UV visible (wavelength range of 350-700nm) and fluorescence spectra (excitation wavelength is 360nm with), with the increase of doping amount of copper, the absorption wavelength and corresponding fluorescence emission wavelength show the red shift phenomenon, at the same time, the fluorescence quantum yield also changes. The preparation method of the invention is simple, green and environment-friendly, and has good application potential in the preparation of LED.

A Fluorescence Particle Spectrometer (FPS) performs real-time measurement of the fluorescence spectra of aerosol particles in the size range 1-10 μm diameter. The FPS has a sufficiently high sample rate (estimated to be a few liters / min) to measure aerosol within buildings (from 1 up to 600 particle fluorescence spectra per minute) at practical rates. A virtual impactor first concentrates aerosol particles, which are then drawn under negative pressure through an aerodynamic focusing nozzle in the inlet of the instrument, through the sample region, providing further concentration. The rate of particle spectra measured by the FPS increases significantly when the particle inlet is within a few meters of some common sources of indoor biological particles.

The invention discloses 2-micron luminous rare-earth ion-doped germanate laser glass and a preparation method thereof. The mol percentage composition of the glass is as follows: 60 to 65 percent of GeO2, 15 to 20 percent of BaF2, 2 to 13 percent of Ga2O3, and 2 to 13 percent of Re2O3ReF3; wherein, Re is the rare-earth elements of La, Yb, Tm and Ho. The rare-earth ion doping mode of the glass is: single doping of Tm, double doping of Yb and Tm, double doping of Yb and Ho, double doping of Tm and Ho, or three doping of Yb, Tm and Ho. Rare-earth ions are introduced in the form of rare earth oxides or rare earth fluorides and can all obtain the glass with stability and excellent luminous performance. Test result shows that the germanate glass in the invention has higher transmission rate and wider transmission range. A fluorescence spectrum test shows that the germanate laser glass of the invention can obtain excellent fluorescence emitting approaching to 2-micron. The laser glass of the invention is also suitable for preparing 2-micron optical fiber perform cores.

The invention relates to a target molecule detection method based on nanometer-gold and nucleic acid structure and a kit thereof. The target molecule detection method comprises the following steps of: (1) reacting specific DNA which can be specifically combined with the target molecules with a solution to be detected; (2) mixing the reaction solution obtained in the step (1) with a nanometer-gold solution for reaction; and (3) observing the fluorescence spectrum of the reaction solution finished in the step (2). The detection method has generality, the detected object can be an arbitrary target molecule, and the application range is wide. By utilizing the invention, the target molecules can be fast, sensitively and selectively detected.

The invention relates to a fluorescence enhancement type hydrogen sulfide fluorescent probe and particularly relates to a fluorescence enhancement type hydrogen sulfide fluorescent probe based on flavone derivatives. Flavone derivatives are directly reacted with 2,4-dinitrofluorobenzene, and a reaction product is subjected to column chromatography to generate a pure probe product. According to the fluorescence enhancement type hydrogen sulfide fluorescent probe, probe molecules have the maximum absorption wavelengths of 460nm, relatively good solubility in a 1.5mM CTAB water solution and stable optical performance; by adding the probe molecules together with hydrogen sulfide, the absorption wavelengths are blueshifted from 460nm to 450nm, and the intensity of a fluorescence spectrum is continuously enhanced at 612nm and is maximally enhanced by 96 times. According to the fluorescence enhancement type hydrogen sulfide fluorescent probe, the deficiencies that an existing fluorescent probe is slow in response to hydrogen sulfide and high in detection line, and detection wavelengths are in a visible light region are overcome; the probe molecules are high in sensitivity, stable in optical performance, relatively high in synthetic yield, strong in capability of identifying hydrogen sulfide, high in response speed, wide in detection range and low in detection lower limit, the response range is 0-50 mu M, and the detection limit is 0.09 mu M; therefore, the fluorescent probe has practical application values in the fields of biological chemistry, environmental science and the like.

The invention discloses a rhodamine derivative and a preparation method and application thereof. The rhodamine derivative is prepared by reacting rhodamine hydrazide and 4-chloro-7-nitro-2,1,3-benzoxaoxadiazole in the presence of an acid binding agent. The Cu<2+> and Fe<3+> can be respectively detected at high selectivity and high sensitivity. The designed and synthesized structure is short in synthetic route and mild in reaction conditions, the obtained derivative can be used for an aqueous phase system, and the ultraviolet-visible absorption spectrum and fluorescence spectrum are insensitive to the acid-base property of the system.

The invention relates to a method for comprehensively testing the stability of a deep ultraviolet optical element. The method comprises the following steps of: measuring the absorption loss absolute value of the deep ultraviolet optical element by adopting a laser calorimetry technology and calibrating a photo-thermal signal amplitude; monitoring real-time change of the absorption loss of the deep ultraviolet optical element in the deep ultraviolet laser irradiation process by adopting a photo-thermal technology; measuring a fluorescence spectrum of the deep ultraviolet optical element and real-time change of the fluorescence spectrum by adopting a laser induction fluorescent technology; and monitoring the performance stability of the deep ultraviolet optical element by measuring the absorption loss of the deep ultraviolet optical element and the real-time change of the fluorescence spectrum.

The invention relates to a detection apparatus (30) for determining a state of tissue. The detection apparatus (30) comprises a fluorescence spectrum providing unit (2, 3) for providing a fluorescence spectrum of the tissue, a differentiation unit (4) for generating a derivative of the fluorescence spectrum, and a tissue state determination unit (5) for determining the state of the tissue from the derivative. This allows determining the state of the tissue, even if characteristics, which are related to the state of the tissue, are only hardly visible or not visible at all in the fluorescence spectrum. The reliability of determining the state of the tissue, for instance, whether the tissue is cancerous or not, can therefore be improved.

The invention discloses a multichannel fluorescence spectra biological aerosol particle detecting apparatus. The apparatus comprises the following components: an aerosol air-flow generation unit for generating aerosol particle gas; a laser detection unit, which is arranged corresponding to the aerosol air-flow generation unit, in order to carry out laser irradiation and detection of partial aerosol particle gas outputted from the aerosol air-flow generation unit, and to obtain diffusion light signals and intrinsic fluorescence signals of the aerosol particles; a total flow controlling and exhausting unit, which is arranged corresponding to the aerosol air-flow generation unit, in order to carry out filtering and discharge of aerosol particles which are outputted from the aerosol air-flow generation unit and do not pass through the laser detection unit and aerosol particles which pass through the laser detection unit; an analyzing unit, which is used for accurately determining classes of the aerosol particles according to the diffusion light signals and the intrinsic fluorescence signals of the aerosol particles.

The present disclosure describes a method of detecting a drug marker in urine a urine sample using a toilet. The drug markers are fluorophores each of which emits a unique fluorescence spectra. Accordingly, the method does not detect the drug but rather, the drug marker. A user who has consumed the drug with its unique drug marker then urinates into the toilet and a urine sample is captured. The toilet includes a mechanism for fluid handling which diverts urine into a fluorescence spectrometer. The fluorescence spectrometer screens the urine for drug markers based on their unique fluorescent spectra. The toilet may include a controller which quantifies the drug marker. The fluorescent spectrometer may detect multiple drug markers in a single urine sample. This method may be used to confirm drug compliance, test for illicit drugs, identify amounts of drugs consumed, and other uses described herein.

The invention discloses an iron oxide / bismuth tungstate composite photocatalyst, a preparation method of the iron oxide / bismuth tungstate composite photocatalyst and the application of the iron oxide / bismuth tungstate composite photocatalyst. The iron oxide / bismuth tungstate composite photocatalyst has a Fe2O3 / Bi2WO6 XRD (X-ray diffraction) diffraction pattern as shown in Figure 1 of the specification and a composite morphology as shown in Figure 2 of the specification. 10-30nm of Fe2O3 nano-particles are precipitated on the surface of Bi2WO6 by a soaking-low temperature roasting technology. The invention represents the change of the structure, the morphology, the ultraviolet-visible absorption spectrum and the fluorescence spectrum of the material before and after being modified in details, and researches the catalytic degradation of the visible light of the iron oxide / bismuth tungstate composite photocatalyst to the activity of the dyestuff rhodamine B (RhB). The result shows that Fe2O3 particles with the mean grain sizes of 20nm are dispersed on the surface of a nanosheet unit of a Bi2WO6 superstructure, the visible light response range of the Fe2O3 / Bi2WO6 composite photocatalyst is obvious in bathochromic shift, and the composition probability of photon-generated carriers can be effectively restrained as well. When the toasting temperature is low temperature of 200DGE C, and the Fe element content is 0.1% (wt%), the photocatalytic activity is optimal, and the efficiency of the visible-light catalytic degradation RhB can be improved by 20% compared with that of the un-composited visible-light catalytic degradation RhB.

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

The invention relates to a carbon quantum dot-copper ion fluorescent probe and a preparation method and application thereof. The method includes that carbon quantum dots with strong fluorescence are synthesized by the aid of a microwave hydrothermal method, fluorescence strength of the carbon quantum dots are greatly reduced by the aid of copper ions, the copper ions are composited on surfaces ofthe carbon quantum dots, surface energy is transferred, CQDs / Cu<2+> with weak fluorescence is formed, a glucose solution is added, pH value is adjusted to 7, heating is performed at the temperature of80DEG C, glucose and the copper ions undergo 'copper mirror reaction' to be oxidized to gluconic acid, and the copper ions are reduced to be precipitated to be Cu2O2. The fluorescence spectrum test shows that after the glucose solution is added, glucose is catalyzed and oxidized through the copper ions on the carbon quantum dots CQDs for reaction and is removed from surfaces of the CQDs, and thestrong fluorescence on the CQDs is restored. The carbon quantum dot-copper ion fluorescent probe based CQDs / Cu<2+> is applicable to effective detection of the glucose under non-enzymatic environment.

The invention discloses a method for tracing, classifying and identifying a polluted water body by utilizing fluorescence spectrum features. The classification and identification method comprises thefollowing steps: extracting features according to three-dimensional fluorescence spectrum data, constructing a basic fluorescence spectrum database and a feature fluorescence spectrum database, constructing a basic model of fluorescence spectrum clustering by using a K-means algorithm, and taking a K-means clustering result as a mode layer and a summation layer of a PNN probabilistic neural network for training to obtain a category to which an input sample belongs. On the basis, the PNN neural network is utilized to predict an unknown sewage sample to judge the affiliation category of the unknown sewage sample. In order to obtain unknown sample matching information, basic fluorescence spectrum data of all known samples in the same category of the unknown sample and feature spectral data are brought into a comparison recognition library, the similarity matching degrees of the unknown sample and all known samples in the same category are obtained through similarity comparison calculation, the matching with a highest matching item score is optimal matching of the two samples, and the suspected traceability information of the unknown sample is obtained.

The invention discloses a citrus vein phloem degeneration detection and classification method based on the D-S theory through multi-source data fusion. The method includes utilizing a hyperspectral image collecting platform, a visible spectrum image collecting platform and a fluorescence spectra image collecting platform to collect image information of citrus leaf samples, performing classification of the symptoms of the citrus vein phloem degeneration on the various spectrum collected image information, and acquiring the identification rate of each classification of the symptoms of three varieties of spectrum; allowing the identification rates of three varieties of spectrum to serve as evidence sources, allocating PBA values to each proposition of an evidence identifying frame, and establishing credibility vectors of the evidence sources; allowing the credibility to serve as a discount factor to adjust the BPA values to each proposition of the evidence identifying frame; fusing the adjusted BPA values according to a Dempster combination rule, acquiring a comprehensive identification rate of the symptoms of the citrus vein phloem degeneration, and implementing correct diagnosis of the symptoms of the citrus vein phloem degeneration. According to the method, the identification rates of the hyperspectrum, visible spectrum and fluorescence spectra are fused, the comprehensive identification rate of the symptoms of the citrus vein phloem degeneration is acquired, and the correct diagnosis of the symptoms of the citrus vein phloem degeneration is implemented.

The invention discloses a double-signal lung cancer exhalation gas detecting system which comprises a fluorescence detection device, a visible light detection device, a gas reaction device and a spectrograph, wherein the fluorescence detection device comprises a fiber-optic probe, an excitation light source supporting table, a motor I, an excitation light source transfer table, an optical filter installation plate and a rotation shaft I; the visible light detection device comprises a light-emitting diode (LED) light source plate and a camera; the gas reaction device comprises a motor II, a gas reaction chamber, a reaction table, a rotation shaft II, a porphyrin sensor thin piece, an air pump and a driving mechanism driving the motor II to horizontally move; the gas reaction chamber is located between the fluorescence detection device and the visible light detection device; and the gas reaction chamber is higher than the LED light source plate and the excitation light source transfer table and lower than the camera and the optical filter installation plate. With the adoption of the double-signal lung cancer exhalation gas detecting system, two kinds of signals including fluorescence spectra and visible light colors are adopted to measure simultaneously, detection is carried out through cross response, and the detection is rapid and high in sensitivity, so that detection accuracy is greatly improved, the price is low, and the operation is simple.

The invention provides a fluorescent probe compound used for detection of acetate, and a preparation method and applications thereof, and belongs to the field of fluorescent probe compounds and preparation of fluorescent probe compounds. The fluorescent probe compound N, N'-2-[3-hydroxy-4-(2-benzoxazole)phenyl]carbamide can be used as a ratiometric fluorescent identification probe for concentration detection of acetate, and is used for detecting fluorescence peak intensity at 470nm and fluorescence intensity at 380nm on a fluorescence spectra. Sensitivity, and selectivity of the fluorescent probe compound on acetate detection are relatively high, and reproducibility is excellent. The fluorescent probe compound can be used for acetate detection in the fields of biochemistry, environmental chemistry, and the like.

The object of the present invention is to provide a substance, which is easy to handle and enables the measurement of ATP with a high sensitivity regardless of the concentration of protein, and further a measuring method of ATP using the substance. Such object is solved with a fluorescence labelled fusion protein obtained by attaching two types of fluorescent substances of potential donor and acceptor for fluorescence resonance energy transfer (FRET) respectively to a protein which can cause structural changes depending on ATP binding, namely ε protein, which is the subunit of ATP synthetase, and further solved by contacting the fluorescence labelled fusion protein with a subject substance and then measuring the fluorescence spectra.