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Preparation method of liposome embedded quantum dots silicon dioxide microspheres and products thereof

A silicon dioxide and liposome technology, applied in the preparation of microspheres, preparations for in vivo tests, chemical instruments and methods, etc., can solve the problem of only a small amount of quantum dots can be embedded, and overcome the uneven particle size distribution , Improve the intensity of light signal, reduce the effect of toxicity

Inactive Publication Date: 2009-02-11
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defect that silica microspheres can only embed a small amount of quantum dots in the current method, and provide a liposome-embedded quantum dot that can embed a large amount of quantum dots and has a relatively uniform particle size distribution. The preparation method of silica microsphere

Method used

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  • Preparation method of liposome embedded quantum dots silicon dioxide microspheres and products thereof
  • Preparation method of liposome embedded quantum dots silicon dioxide microspheres and products thereof
  • Preparation method of liposome embedded quantum dots silicon dioxide microspheres and products thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Preparation of silica nanospheres with liposome-embedded quantum dots:

[0050] The preparation of step A, lipid film:

[0051] Accurately weigh soybean phospholipids and cholesterol (mass ratio is 3:2), dissolve phospholipids and cholesterol in a pear-shaped flask with chloroform, rotate to evaporate, evaporate chloroform to make phospholipids and cholesterol form a lipid film on the wall of the flask, blow with nitrogen Dry the film to remove residual chloroform;

[0052] The preparation of step B, liposome:

[0053] Red fluorescent CdTe / CdS quantum dot aqueous solution (basic pH=10) was added to the lipid film, soaked overnight under nitrogen protection, and then shaken for about 1 hour to obtain a liposome suspension embedding CdTe / CdS. In the suspension, the appearance is turbid due to the large liposome particles;

[0054] Step C, embedding of silica:

[0055] (1) Take part of the liposome suspension of CdTe / CdS, filter it with ordinary qualitative filter pape...

Embodiment 2

[0059] Preparation of silica microspheres embedded with quantum dots in water-soluble drug liposomes:

[0060] The preparation of step A, lipid film:

[0061] Accurately weigh soybean phospholipids and cholesterol (mass ratio is 3:2), dissolve phospholipids and cholesterol in a plow flask with chloroform, rotate to evaporate, evaporate chloroform to make phospholipids and cholesterol form a lipid film on the wall of the flask, blow with nitrogen Dry the film to remove residual chloroform;

[0062] The preparation of step B, water-soluble drug liposome:

[0063] Add CdTe / CdS quantum dot aqueous solution and doxorubicin (water-soluble drug) to the lipid film, soak overnight under nitrogen protection, and shake for about 1 hour to obtain a water-soluble drug liposome mixture embedding CdTe / CdS quantum dots and drug. suspension;

[0064] Step C, embedding of silica:

[0065] Use ordinary qualitative filter paper to filter to remove large liposome aggregates, then directly add ...

Embodiment 3

[0068] Preparation of silica microspheres with lipid-soluble drug liposomes embedding quantum dots:

[0069] Step A, preparation of fat-soluble drug lipid film:

[0070] Accurately weigh soybean phospholipids and cholesterol (mass ratio is 3:2) and paclitaxel (fat-soluble drug), dissolve phospholipids and cholesterol in a plow flask with chloroform, add paclitaxel, rotary evaporate, and evaporate chloroform to make phospholipids, cholesterol and Paclitaxel forms a fat-soluble drug lipid film on the wall of the flask, and nitrogen blows the film to remove residual chloroform;

[0071] The preparation of step B, fat-soluble drug liposome:

[0072] Add CdTe / CdS quantum dot aqueous solution to the lipid film, soak overnight under nitrogen protection, and then shake for about 1 hour to obtain a fat-soluble drug liposome suspension embedding CdTe / CdS quantum dots and drugs;

[0073] Step C, embedding of silica:

[0074] Use ordinary qualitative filter paper to filter to remove la...

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Abstract

The invention relates to a method for preparing silicon-dioxide microspheres with liposome embedded with quantum dots. The method includes the following steps: lipid material is dissolved in an organic solvent for being prepared to be a lipid film by rotary evaporation; the lipid film is added with a water solvent of the quantum dots for being prepared to be the liposome taking lipid as the film and the quantum dots as the core and with a grain size less than or equal to 10 Mum by a thin film dispersing method; the lipsome is added with tetraethyl orthosilicate to form silicon dioxide on the surface of the lipsome so as to obtain the silicon-dioxide microsphere with the liposome embedded with the quantum dots. The method has the advantages that: the silicon-dioxide microsphere with the liposome embedded with the quantum dots can decrease the toxicity of the quantum dots, increase the light signal intensity of every luminous point, and obtain microspheres with large quantity and different fuorescence spectra characteristics so as to play a part in biological high-flux detection. The quantum-dot microsphere of micron size is applicable to the cure of tumour artery embolism; and the quantum-dot silicon-dioxide microspheres with different grain sizes evenly arranging from nanometer to micron can be obtained by the method.

Description

field of invention [0001] The preparation method of liposome-embedded silicon dioxide microspheres with quantum dots and the products thereof of the present invention relate to a method for preparing silicon dioxide microspheres and nanospheres embedded with a large amount of quantum dots, especially involving lipids Silica microspheres and nanospheres obtained by embedding quantum dot nanoparticles in liposomes, or embedding quantum dots and drugs at the same time, and then forming silica on the surface of liposomes, which can be used for biological Fluorescent labeling, or arterial embolization and imaging, belongs to the field of materials and biomedicine. Background technique [0002] Quantum dots are semiconductor nanocrystals, whose scale is mainly distributed in the range of 1 to 10nm. Due to their superior fluorescence compared to conventional organic fluorescent dyes, such as relatively stable fluorescence, high quantum yield (that is, very bright fluorescence), qua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C09K11/54C09K11/56C09K11/88A61K49/00
Inventor 储茂泉
Owner TONGJI UNIV
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