1101results about "Raman/scattering spectroscopy" patented technology

For angular resolved spectrometry a radiation beam is used having an illumination profile having four quadrants is used. The first and third quadrants are illuminated whereas the second and fourth quadrants aren't illuminated. The resulting pupil plane is thus also divided into four quadrants with only the zeroth order diffraction pattern appearing in the first and third quadrants and only the first order diffraction pattern appearing in the second and third quadrants.

A spectral bio-imaging method for enhancing pathologic, physiologic, metabolic and health related spectral signatures of an eye tissue, the method comprising the steps of (a) providing an optical device for eye inspection being optically connected to a spectral imager; (b) illuminating the eye tissue with light via the iris, viewing the eye tissue through the optical device and spectral imager and obtaining a spectrum of light for each pixel of the eye tissue; and (c) attributing each of the pixels a color or intensity according to its spectral signature, thereby providing an image enhancing the spectral signatures of the eye tissue.

A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and / or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophore or combinations of fluorophores.

Fourier domain a / LCI (faLCI) system and method which enables in vivo data acquisition at rapid rates using a single scan. Angle-resolved and depth-resolved spectra information is obtained with one scan. The reference arm can remain fixed with respect to the sample due to only one scan required. A reference signal and a reflected sample signal are cross-correlated and dispersed at a multitude of reflected angles off of the sample, thereby representing reflections from a multitude of points on the sample at the same time in parallel. Information about all depths of the sample at each of the multitude of different points on the sample can be obtained with one scan on the order of approximately 40 milliseconds. From the spatial, cross-correlated reference signal, structural (size) information can also be obtained using techniques that allow size information of scatterers to be obtained from angle-resolved data.

In one aspect the invention relates to an apparatus for analyzing the presence of a single molecule using total internal reflection. In one embodiment an apparatus for single molecule analysis includes a support having a sample located thereon; two sources of light at distinct wavelengths, a collimator for directing the light onto the sample through a total internal reflection objective; a receiver for receiving a fluorescent emission produced by a single molecule in the sample in response to the light; and a detector for detecting each of the wavelengths in the fluorescent emission. In another embodiment the apparatus further comprises a focusing laser for maintaining focus of the objective on the sample.

Exemplary systems and methods for obtaining information associated with at least one portion of a sample can be provided. For example, a first radiation can be received and at least one second radiation and at least one third radiation can be provided as a function of the first radiation. Respective intensities of the second and third radiations can be modulated, whereas the second and third radiations may have different modulation frequencies, and the modulated second and third radiations can be directed toward the portion. The photoluminescence radiation can be received from the portion based on the modulated second and third radiations to generate a resultant signal. The signal can be processed to obtain the information which is / are photoluminescence lifetime characteristics and / or a polarization anisotropy of the portion. According to another exemplary embodiment, the photoluminescence radiation can be received and the photoluminescence radiation may be based on wavelengths thereof.

A color display comprising an image of all chromosomes or portions of chromosomes of a cell, each of the chromosomes or portions of chromosomes being painted with a different fluorophore or a combination of fluorophores, the image presenting the chromosomes or portions of chromosomes in different distinctive colors, wherein each of the chromosomes or portions of chromosomes is associated with one of the different distinctive colors.

An endoscope having an optical guide that is optically coupled to a first broadband light source and a second laser light source that emits light at a wavelength in a range of 350 nm to 420 nm. The endoscope has an image sensor at a distal end and collects a reflectance image including red, green and blue components with the image sensor in response to illumination by said broadband light source. The image sensor also collects an autofluorescence image having a blue component, a green component and a red component. A processor processes the fluorescence image by determining a ratio of the fluorescence image and the reflectance image to provide a processed fluorescence image.

A method and an apparatus for noninvasively and quantitatively determining spatially resolved absorption and reduced scattering coefficients over a wide field-of-view of a food object, including fruit or produce, uses spatial-frequency-domain imaging (SFDI). A single modulated imaging platform is employed. It includes a broadband light source, a digital micromirror optically coupled to the light source to control a modulated light pattern directed onto the food object at a plurality of selected spatial frequencies, a multispectral camera for taking a spectral image of a reflected modulated light pattern from the food object, a spectrally variable filter optically coupled between the food object and the multispectral camera to select a discrete number of wavelengths for image capture, and a computer coupled to the digital micromirror, camera and variable filter to enable acquisition of the reflected modulated light pattern at the selected spatial frequencies.

An apparatus for imaging a tooth has at least one illumination source for providing an incident light having a first spectral range for obtaining a reflectance image of the tooth and a second spectral range for exciting a fluorescence image of the tooth. A first polarizer having a first polarization axis and a compensator in the path of the incident light of the first spectral range are disposed to direct light toward the tooth. A second polarizer is disposed to direct light obtained from the tooth toward a sensor and has a second polarization axis that is orthogonal to the first polarization axis. A lens is positioned in the return path to direct image-bearing light from the tooth toward the sensor for obtaining image data. A filter in the path of the image-bearing light from the tooth is treated to attenuate light in the second spectral range.

A spectral bio-imaging method for enhancing pathologic, physiologic, metabolic and health related spectral signatures of an eye tissue, the method comprising the steps of (a) providing an optical device for eye inspection being optically connected to a spectral imager; (b) illuminating the eye tissue with light via the iris, viewing the eye tissue through the optical device and spectral imager and obtaining a spectrum of light for each pixel of the eye tissue; and (c) attributing each of the pixels a color according to its spectral signature, thereby providing an image enhancing the spectral signatures of the eye tissue.

A method for investigating specimens, wherein a spectral splitting of the radiation coming from the specimen is carried out for specimen points or point distributions, for the operation of a laser scanning microscope or a fluorescence screening arrangement or a flow cylinder comprising the steps of generating a λ-stack so that the spectral distribution is measured by individual detection channels and storing the signals so as to be correlated to the detection signals with at least one of the spatial coordinates x, y and z and / or so as to be correlated to the measurement time t.

Exemplary embodiments of methods, arrangements and systems for obtaining information about a sample can be provided. For example, in one exemplary embodiment, it is possible to receive a first electro-magnetic radiation from a sample which is based on a second electro-magnetic radiation forwarded to the sample. The first electro-magnetic radiation may have a first frequency and the second electro-magnetic radiation may have a second frequency which is different from the first frequency. The difference between the first and second frequencies can be based on an acoustic wave inside the sample related to at least one characteristic of the sample. Further, it is possible to receive at least a portion of the first electromagnetic radiation and separate it into a particular finite number (N) of frequency component radiations. In addition, it is possible to receive a particular energy of more than 1 / N of energy of the third electro-magnetic radiation, and generate information associated with the sample. Certain exemplary embodiments of the present invention are capable of obtaining information associated with a sample, particularly its mechanical properties, non-contact using electromagnetic radiation.

The present invention provides a fluorescence detection and photodynamic therapy apparatus including: a combined light source unit 10 including a plurality of coherent and non-coherent light sources 11, 12 and 13 configured to irradiate light onto a to-be-observed object while performing continuous illumination; an optical imaging unit 20 configured to form an image of the to-be-observed object 70 and project the image to an image processing / controlling system 34; a multispectral imaging unit 30 including a one-chip multispectral sensor and the image processing / controlling system 34; a blocking filter 40 installed between the to-be-observed object 70 and the one-chip multispectral sensor 32, the blocking filter being configured to block some light reflected off from the to-be-observed object 70 while allowing some light and fluorescent light to pass therethrough; a computer system 50 configured to process, analyze, reproduce and store the image acquired from the multispectral imaging unit 30, and transfer the image to a display device 60 and control the overall operation of all the related elements; and the display device 60 configured to display a processing result of the image by the computer system 50.

Systems, methods, and apparatuses of low-coherence enhanced backscattering spectroscopy are described within this application. One embodiment includes providing incident light comprising at least one spectral component having low coherence, wherein the incident light is to be illuminated on a target object in vivo. An intensity of one or more of at least one spectral component and at least one angular component of backscattering angle of backscattered light is recorded, wherein the backscattered light is to be backscattered from illumination of the incident light on the target object and wherein the backscattering angle is an angle between incident light propagation direction and backscattered light propagation direction. The intensity of the at least one spectral component and the at least one backscattering angle of backscattered light is analyzed, to obtain one or more optical markers of the backscattered light, toward evaluating said properties.

A fluorescence imaging system for imaging an object, the system includes a white light provider that emits white light, an excitation light provider that emits excitation light in a plurality of excitation wavebands for causing the object to emit fluorescent light, a component that directs the white light and excitation light to the object and collects reflected white light and emitted fluorescent light from the object, a filter that blocks light in the excitation wavebands and transmits at least a portion of the reflected white light and fluorescent light, and an image sensor assembly that receives the transmitted reflected white light and the fluorescent light.

A device to provide hyperspectral reflection spectrum, hyperspectral depolarization, and hyperspectral fluorescence spectrum data in a portable, remote sensing instrument. The device can provide a large range of remotely-sensed optical property data, presently only obtainable in laboratories, in a low-cost field instrument. Among its many uses, the present invention can be used by farmers as a tool for determining the nitrogen content of crops to optimize fertilizer laydown.

The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.

A method of spectral-morphometric analysis of biological samples, the biological samples including substantially constant components and suspected variable components, the method is effected by following the steps of (a) using a spectral data collection device for collecting spectral data of picture elements of the biological samples; (b) defining a spectral vector associated with picture elements representing a constant component of at least one of the biological samples; (c) using the spectral vector for defining a correcting function being selected such that when operated on spectral vectors associated with picture elements representing other constant components, spectral vectors of the other constant components are modified to substantially resemble the spectral vector; (d) operating the correcting function on spectral vectors associated with at least the variable components for obtaining corrected spectral vectors thereof; and (e) classifying the corrected spectral vectors into classification groups.

A method for remote scenes classification comprising the steps of (a) preparing a reference template for classification of the remote scenes via (i) classifying a set of reference scenes via a conventional classification technique for obtaining a set of preclassified reference scenes; (ii) using a first spectral imager for measuring a spectral cube of the preclassified reference scenes; (iii) employing a principal component analysis for extracting the spectral cube for decorrelated spectral data characterizing the reference scenes; and (vi) using at least a part of the decorrelated spectral data for the preparation of the reference template for remote scenes classification; (b) using a second spectral imager for measuring a spectral cube of analyzed remote scenes, such that a spectrum of each pixel in the remote scenes is obtained; (c) employing a decorrelation statistical method for extracting decorrelated spectral data characterizing the pixels; and (d) comparing at least a part of the decorrelated spectral data extracted from the pixels of the remote scenes with the reference template.

Improved methods, systems and apparatus relating to wide field fluorescence and reflectance imaging are provided, including improved methods, systems and apparatus relating to removal of background signals such as autofluorescence and / or fluorophore emission cross-talk; distance compensation of fluorescent signals; and co-registration of multiple signals emitted from three dimensional tissues.

The present invention provides an apparatus for photodynamic therapy and fluorescence detection, in which a combined light source is provided to illuminate an object body and a multispectral fluorescence-reflectance image is provided to reproduce various and complex spectral images for an object tissue, thus performing effective photodynamic therapy for various diseases both outside and inside of the body.For this purpose, the present invention provides an apparatus for photodynamic therapy and photodetection, which provides illumination with light of various wavelengths and multispectral images, the apparatus including: an optical imaging system producing an image of an object tissue and transmitting the image to a naked eye or an imaging device; a combined light source including a plurality of coherent and non-coherent light sources and a light guide guiding incident light emitted from the light sources; a multispectral imaging system including at least one image sensor; and a computer system outputting an image of the object tissue to the outside. Thus, the apparatus for photodynamic therapy and photodetection of the present invention can effectively perform the photodynamic therapy and photodetection by means of the combined light source capable of irradiating light having various spectral components to an object tissue and the multispectral imaging system capable of obtaining images from several spectral portions for these various spectral ranges at the same time, thus improving the accuracy of diagnosis and efficiency of the photodynamic therapy.

An apparatus includes an optical element, a GRIN lens, and a detector. The optical element has a first optical aperture. The GRIN lens has first and second ends. The first end of the GRIN lens is positioned to receive light from the first optical aperture. The detector is configured to measure values of a characteristic of light emitted from the first end in response to multi-photon absorption events in a sample illuminated by light from the second end of the endoscopic probe.

The disclosure generally relates to a method and apparatus for compact dispersive imaging spectrometer. More specifically, one embodiment of the disclosure relates to a portable system for obtaining a spatially accurate wavelength-resolved image of a sample having a first and a second spatial dimension. The portable system can include a photon emission source for sequentially illuminating a plurality of portions of said sample with a plurality of photons to produce photons scattered by the sample. The photon emission source can illuminate the sample along the first spatial dimension for each of plural predetermined positions of the second spatial dimension. The system may also include an optical lens for collecting the scattered photons to produce therefrom filtered photons, a dispersive spectrometer for determining a wavelength of ones of the filtered photons, a photon detector for receiving the filtered photons and obtaining therefrom plural spectra of said sample, and a processor for producing a two dimensional image of said sample from the plural spectra.

The invention relates to apparatus and methods for assessing occurrence of a hazardous agent in a sample by performing multimodal spectral analysis of the sample. Methods of employing Raman spectroscopy for entities in a sample which exhibit one or more optical properties characteristic of a hazardous agent are disclosed. Devices and systems suitable for performing such methods are also disclosed.

A time-resolved, fluorescence spectrometer makes use of a RadiaLight® optical switch and no dispersive optical elements (DOE) like gratings. The structure is unique in its compactness and simplicity of operation. In one embodiment, the spectrometer makes use of only one photo-detector and an efficient linear regression algorithm. The structure offers a time resolution, for multiple species measurements, of less than 1 s. The structure can also be used to perform fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

A system for time-correlated photon counting. The system uses one or more photon detectors to produce electrical pulses corresponding to photons read from a target. The system uses a discriminator with a first input coupled to a trigger output from a pulsed optical source and a second input for receiving the electrical pulses. A time-to-pulse height converter is used for producing a series of difference signals each with a respective maxima and whose magnitude is related to the time difference between the trigger output and the electrical pulses. In addition, the system employs a pulse shaping electronic circuit for receiving pulsed electrical output and producing a series of one or more characteristic signals. An A / D converter with a first input receives the difference signals and a second input receives part of the characteristic signals. The A / D converter produces a first series of digital signals representing the difference signals and a second series of digital signals representing the characteristics signals. The results of the A / D converter are feed to a multichannel analyzer for time-shifting the first series of digital signals based on at the second series of digital signals so that the maxima for any given difference signals occurs at the same time as the maxima for at least one other part of the series of the difference signals.