327 results about "Bio imaging" patented technology

A spectral bio-imaging method for enhancing pathologic, physiologic, metabolic and health related spectral signatures of an eye tissue, the method comprising the steps of (a) providing an optical device for eye inspection being optically connected to a spectral imager; (b) illuminating the eye tissue with light via the iris, viewing the eye tissue through the optical device and spectral imager and obtaining a spectrum of light for each pixel of the eye tissue; and (c) attributing each of the pixels a color or intensity according to its spectral signature, thereby providing an image enhancing the spectral signatures of the eye tissue.

A spectral bio-imaging method for enhancing pathologic, physiologic, metabolic and health related spectral signatures of an eye tissue, the method comprising the steps of (a) providing an optical device for eye inspection being optically connected to a spectral imager; (b) illuminating the eye tissue with light via the iris, viewing the eye tissue through the optical device and spectral imager and obtaining a spectrum of light for each pixel of the eye tissue; and (c) attributing each of the pixels a color according to its spectral signature, thereby providing an image enhancing the spectral signatures of the eye tissue.

Described herein are catheters for use with Optical Coherence Tomography (OCT) that include an optical fiber core having a first refractive index and an interface medium having a second refractive index, where the first and second refractive indexes are mismatched such that receiving electronics configured to receive optical radiation reflected from the reference interface and the target operate in a total noise range that is within 5 dB of the shot noise limit. These OCT catheters may include a silicon die mirror having a reflective coating that is embedded in the interface medium. The optical fiber can be fixed at just the distal end of the catheter, and may be managed within a handle that is attached to the proximal end of the catheter body, and is configured to allow rotation of the both catheter body and the optical fiber relative to the handle.

A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health. The system includes an enclosure for placement around a plant to be imaged in-situ. There is a controlled light source that controllably provides to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure. The chlorophyll fluorescence emitted from the plant both spatially and temporally is captured, and the captured fluorescence information is analyzed to provide plant health information.

A method of spectral-morphometric analysis of biological samples, the biological samples including substantially constant components and suspected variable components, the method is effected by following the steps of (a) using a spectral data collection device for collecting spectral data of picture elements of the biological samples; (b) defining a spectral vector associated with picture elements representing a constant component of at least one of the biological samples; (c) using the spectral vector for defining a correcting function being selected such that when operated on spectral vectors associated with picture elements representing other constant components, spectral vectors of the other constant components are modified to substantially resemble the spectral vector; (d) operating the correcting function on spectral vectors associated with at least the variable components for obtaining corrected spectral vectors thereof; and (e) classifying the corrected spectral vectors into classification groups.

A spectral bio-imaging method for enhancing pathologic, physiologic, metabolic and health related spectral signatures of an eye tissue, the method comprising the steps of (a) providing an optical device for eye inspection being optically connected to a spectral imager; (b) illuminating the eye tissue with light via the iris, viewing the eye tissue through the optical device and spectral imager and obtaining a spectrum of light for each pixel of the eye tissue; and (c) attributing each of the pixels a color according to its spectral signature, thereby providing an image enhancing the spectral signatures of the eye tissue.

The invention discloses a fluorine-boron fluorescent dye as well as a preparation method and application thereof, wherein the structure of the fluorine-boron fluorescent dye is shown as a formula (I) or a formula (II), in the formula (I) and the formula (II), R1 is H or halogen; R2 is CN; R3, R4, R5 and R6 are independently selected from H, halogen, C1-C6 alkyl or C1-C6 alkoxy; V, W, X, Y and Z are independently CH or N, and when V, W, X, Y or Z is N, N has no substituent group. According to the fluorine-boron fluorescent dye and the preparation method thereof, the maximal fluorescence emission wavelength of the fluorine-boron fluorescent dye is 518-600nm, and the fluorine-boron fluorescent dye also has excellent fluorescence quantum yield and Stokes shift, which shows that the fluorine-boron fluorescent dye has good application prospect in the bioanalysis fields of fluorescence labeling, bioimaging and so on; meanwhile, the preparation method is simple in steps, and raw materials can be obtained easily.

The invention discloses a nitrogen-doped carbon nanometer particle as well as a preparation method and application thereof, belongs to the field of nanometer material science and is used for solving the technical problems that fluorescence quenching is easily caused to the aggregative state of the carbon nanometer particle due to surface passivation modifier which is added for the preparation of existing carbon nanometer particles. The nitrogen-doped carbon nanometer particle is prepared through a microwave method by using organic compounds containing polycarboxyl or polyhydroxy as materials and using ammonia water as a solvent and a nitrogen doping source. The invention further provides the application of the nitrogen-doped carbon nanometer particle as fluorescent ink and fluorescent glue. The preparation method disclosed by the invention is simple, low in cost, and convenient to realize large-scale production; the maximal fluorescent quantum efficiency of the solid film formed by the prepared fluorescent glue is as high as 84%; the prepared fluorescent ink is non-toxic, generates no precipitates after being placed for a long time, is strong in fluorescence characteristic and can be applied to various fields such as bio-imaging, biological product identification, information storage, information encryption, counterfeiting prevention, illumination and display, sensing and photovoltaic devices.

A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health. The system includes an enclosure for placement around a plant to be imaged in-situ. There is a controlled light source that controllably provides to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure. The chlorophyll fluorescence emitted from the plant both spatially and temporally is captured, and the captured fluorescence information is analyzed to provide plant health information.

A method for defining an isolation area around an object of interest in a cell culture vessel, the method comprising the steps of: obtaining one or more images of the cell culture vessel using one or more of a plurality of illumination sources, each illumination source being capable of illuminating the vessel from a different direction; selecting an image or combination of images for further processing; applying a circular object detection transformation to identify one or more objects of interest being substantially circular objects in the cell culture vessel, which circular objects of interest are representative of isolated colonies in the cell culture vessel and determining the center of an object of interest; applying a binarizing step to obtain a binarized image of the object of interest and other objects, wherein the center of the binarized image corresponds to the center of the object of interest; iteratively forming concentric circles with increasing radius, wherein the concentric circles are centered on the center of the binarized image; identifying coronas, wherein a corona is delimited by two circles having successive radius values; for each corona: determining the presence and the location of any other object located in the corona to determine the presence and the location of other objects; determining a clearance angle defining an angular sector free of other objects around the object of interest to define the isolation area around the object of interest.

The invention discloses a method for preparing nitrogen and sulfur-doped fluorescent carbon quantum dots. According to the method, citric acid is taken as a carbon source, L-glutathione is taken as a nitrogen-sulfur dopant, a microwave oven is taken as a reaction platform, the carbon quantum dots with fluorescence properties are prepared in a heating manner, the residues and moisture are removed through dialysis and freeze drying, and quantum dot powder is obtained. The nitrogen and sulfur-doped carbon quantum dots are prepared by adopting the method disclosed by the invention; the method is simple in operation, low in cost, high in yield, simple in preparation process and equipment, free of a solvent, and easy to popularize and produce on a large scale, and can be rapidly finished within a short period of time; and the prepared carbon quantum dots have the advantages of good water solubility, high fluorescence properties, photobleaching resistance, adjustable emission spectrum, good biocompatibility, low cytotoxicity and the like, and have a good application prospect in the fields of bioimaging, biosensors and photo-electric devices.

The invention discloses a method of one-step synthesizing fluorescent carbon dots with corncobs as a carbon source and belongs to the technical field of synthesis of fluorescent carbon dots. In the technical scheme, the method of one-step synthesizing the fluorescent carbon dots with the corncobs as the carbon source includes the following steps: mixing pre-treated corncobs, ethanediamine and deionized water uniformly, and performing heating reaction to prepare the fluorescent carbon dots through hydrothermal method, microwave method and ultrasound method; or directly calcining the pre-treated corncobs to obtain the fluorescent carbon dots. The raw materials are easy to obtain. The method is simple in processes, is mild in reaction conditions, is simple in operation and is free of toxic reagents. The fluorescent carbon dots can not only be used as a fluorescent probe to detect substance content but also be used in the researching fields of bio-markers, bio-imaging, photo-catalysis, photo-electronic devices, bio-sensors and the like.

The invention discloses a rare earth-doped lithium lutetium fluoride nano-material, and a preparation method and an application thereof. The material is obtained by doping Yb / Er(20 / 1%) or Yb / Tm(20 / 0.5%) luminescent ions into a lithium lutetium fluoride matrix, can emit visible light under the excitation of 980nm infrared light, and can be used for the up-conversion heterogeneous detection and cell imaging through the connection with biological molecules. Additionally, heavy rare earth ions in the matrix also have a strong X-ray attenuation capability, and can be used as a computer tomography imaging contrast agent. The invention also provides the preparation method of the nano-material. The nano-material has a water solubility and excellent performances, so the nano-material can be applied in the fields of the biological detection, the biological imaging and the like.

The invention discloses N-CQDs (nitrogen-doped carbon quantum dots) with high fluorescence quantum yield and a preparation method of the N-CQDs. Alanine is taken as a carbon source, ethanediamine is taken as a surface passivator, and the N-doped carbon quantum dots with high fluorescence quantum yield is successfully prepared with a simple one-step hydrothermal method. Compared with carbon quantum dots synthesized with other protein or amino acid as a carbon source, the nitrogen content of the prepared N-CQDs can reach 61.1%, the average fluorescence lifetime is 4.43 ns, the highest fluorescence quantum yield can reach 46.2% and approaches the fluorescence quantum yield of the carbon quantum dots prepared with a laser ablation method and an electric arc method, and the N-doped carbon quantum dots have low cytotoxicity and excellent biocompatibility and has wide application value in biosensing and bioimaging. The invention further discloses a high quenching effect of NADH (nicotinamide adenine dinucleotide) on fluorescence of the N-CQDs. On the basis that NADH has high quenching effect on fluorescence of the N-CQDs, high-sensitivity fluorescence biosensing for detecting NADH is established, the linear detection range is as low as 80 mu M, and the limit of detection is 25.1 nM.

The invention belongs to the technical field of functional materials, and concretely relates to a water-soluble boron nitride quantum dot used for biological imaging and proton exchange membranes, and a preparation method thereof. The method is characterized in that the water-soluble boron nitride quantum dot with bright blue fluorescence can be directly prepared by processing boron nitride powder through solvent ultrasonic treatment and solvothermal combination, can be used as a high-efficiency and low-toxicity biological cell imaging probe, and also can be used as a modified proton exchange membrane material. The preparation method has the advantages of simple operation, simple post-treatment, low production cost and easy batch production, and the prepared boron nitride quantum dot has the advantages of obvious fluorescence, wide application, large scale preparation potential and wide commercial application prospect.

Multimodal nanoparticles are nanoparticles containing contrast agents for PAT and one or more of luminescence imaging, x-ray imaging, and / or MRI. The multimodal nanoparticles can have a dielectric core comprising an oxide with a metal coating on the core. The particles can be metal speckled. The multimodal nanoparticles can be used for therapeutic purposes such as ablation of tumors or by neutron capture in addition to use as contrast agents for imaging.

The invention discloses a method for preparing high-yield carbon quantum dots, which comprises the following steps that: (1) ammonium citrate is put into an electric heating furnace, the temperature rises at a heating rate of 5 to 50 DEG C / min, and the reaction is carried out for 2-4h at a temperature of 150 to 250 DEG C to obtain a pyrolysis product; (2) the pyrolysis product is milled into powder and added with ethanol to be stirred and centrifuged to remove insolubles to obtain a brown ethanol solution containing carbon quantum dots; and (3) the drying is carried out to obtain the carbon quantum dots. The process is simple to operate, the source of raw materials is wide, the cost of raw materials is low, the preparation conditions has low requirements and are relatively mild, the yield of obtained carbon quantum dots and the quantum yield are higher, the problems that the carbon quantum dots can not be produced on the scale and the fluorescence quantum yield of the obtained carbon quantum dots is low due to the limit of the process and the raw materials in the traditional preparation method of the carbon quantum dots can be solved, and the method can be applied to the fields such as biomarker, bio-imaging, a photovoltaic device, and a biosensor.

The invention discloses a high-stability water-soluble CsPbX3 perovskite nano-crystalline preparation method, and belongs to the technical field of semiconductor nano-material preparation. Water-soluble polymer particles are dispersed in hexane solvents by a solvent exchange method and then centrifuged to obtain polymer micro-sphere precipitates, CsPbX3 perovskite nano-crystalline dispersed in methylbenzene and hexane mixed solvents is mixed with the polymer micro-sphere precipitates for 24 hours, and perovskite nano-crystalline supported polymer particles are obtained by centrifugation. The polymer-supported perovskite nano-crystalline prepared by the preparation method can be dispersed in water solution, has high stability under different pH (potential of hydrogen) conditions, and the water-soluble nano-particles with fluorescence in a visible region and high fluorescence quantum efficiency are obtained by preparing different perovskite particles and have huge application values in the fields of bio-imaging, display and the like.

The invention provides a method for preparation of water-soluble luminous graphite-phase carbon nitride nano kelp. The water-soluble luminous graphite-phase carbon nitride nano kelp is obtained by high-temperature polycondensation of precursors containing carbon and nitrogen in a mixed molten chloride salt system. The method is universal, and target products can be obtained by adoption of various precursors; the prepared nano kelp is uniform in shape, excellent in dispersibility in water, capable of forming high-concentration stable transparent colloidal solution and stable in existence in both alkaline and weak acidic environments; under excitation of ultraviolet light, solid nano kelp and the colloidal solution thereof both have strong and stable blue photoluminescence. On the basis of excellent photoluminescence performance and water solubility, the nano kelp is expected to serve as fluorescent probes to be applied to the fields of bioimaging, biomedical engineering, analysis, monitoring and the like, and large-scale production is expected to realize. Furthermore, the method for preparation of the water-soluble luminous graphite-phase carbon nitride nano kelp has the advantages of simplicity in operation, environment friendliness, low cost and the like.

Methods of preparing bio-imaging nanoparticles having high dispersibility in an aqueous solution, biocompatibility, and targetability with high yield, by early introduction of an irregular structure are disclosed.

The invention discloses gold nanoclusters as well as a preparation method and an application thereof. The preparation method of the gold nanoclusters comprises steps as follows: a mercapto-cyclodextrin solution, a GSH (glutathione) solution, water and a gold-sourced compound solution are mixed and subjected to a heating reaction under the condition that a sodium hydroxide solution and / or a potassium hydroxide solution exist. The mercapto-cyclodextrin and GSH as ligands react with the gold-sourced compound solution, the gold nanoclusters modified with two ligands are obtained and are used for bio-imaging, two ligands are combined, the gold nanoclusters with different particle sizes can be prepared through adjusting of the adding quantities of the two ligands during synthesis, meanwhile, theprepared gold nanoclusters can emit light with different wavelengths, the sizes and light-emitting wavelength of the gold nanoclusters can be simply and feasibly adjusted, and accordingly, the application of the gold nanoclusters is further extended.

An imaging system for generating images of biological samples having a surface, the system comprising: a sample support for supporting a biological sample in use; a plurality of illumination sources, the plurality of illumination sources being arranged around the sample support and each adapted to illuminate the biological sample, in use, from a different direction; an image capture device for capturing illumination which has impinged on the biological sample to thereby form an image of the sample; wherein at least one of the illumination sources direction is not perpendicular to the surface of the sample.

The invention belongs to the technical field of synthesis and application of fluorescent carbon nanomaterials, and particularly relates to atmospheric micro plasma assisted preparation method and application of an acid resistant carbon quantum dot. The method uses citric acid and ethylenediamine as precursors, employs atmospheric micro plasma for assisting the preparation of acid-resistant carbon quantum dots, and has the advantages of fastness, simpleness, low energy consumption, and no subsequent modification. The prepared carbon quantum dot aqueous solution can be used for writing on filter paper, and the characters can emit blue fluorescence under ultraviolet lamp irradiation. The carbon quantum dot has good acid resistance, the fluorescence intensity is basically unchanged in 3mol / L HNO3 environment system compared to in aqueous solution, and remains stable. The fluorescence intensity of the carbon quantum dot shows linear change over a wide pH range, and is change-reversible, and can be used for pH testing. The carbon quantum dot can be used in fluorescent ink writing, pH testing, metal ion detection, biosensors, biological imaging and other fields.

The present invention discloses bio-imaging nanoparticles through introducing irregular structure early and preparation method thereof. The aim of the invention is to provide a method of preparing bio-imaging nanoparticles, wherein by means of part surface modification, introducing irregular structure into the surface of the nanoparticles, and allowing aggregation and deposition phenomenas in the structure caused by the hydrogen bond attraction inside the nanoparticles, the bio-imaging nanoparticles having high dispersibility in an aqueous solution, biocompatibility, and targetability with high yield, are prepared without loss of nanoparticles.

The invention discloses an up-conversion luminescent material as well as a preparation method and the application thereof. The material is a nanoscale hexagonal disk-shaped fluoride, and has the following chemical general: NaLu1-x-y-zRxYbyMzF4, wherein R is a rare earth element Y or any composition of the Y, Gd and La; M is one of or any composition of the rare earth elements Ho, Er and Tm; x, y and z meet the following conditions that x is greater than or equal to 0 and less than or equal to 0.5, y is greater than or equal to 0.1 and less than or equal to 0.5, and z is greater than or equal to 0 and less than or equal to 0.1; the material is prepared by a solvothermal method, which takes octadecene as a solvent and takes oleic acid as a complexing agent, and can be used on the aspects of storage, transmission, optical display, infrared detection, fluorescent falsification-preventing, fluorescence probe, biosensor, and bio-imaging; the material is stable in performance and regular in morphology; the particle size is obviously smaller than that of beta-NaLuF4 synthesized under the same condition; the luminous intensity is higher than that of the up-conversion luminescent materials of beta-NaYF4 and beta-NaLuF4 which is synthesized under the same condition; the up-conversion luminescent material has the advantages of small size, high luminous efficiency and the like.

The invention discloses an upconvresion composite nano-material as well as a preparation method and application thereof. The nano-material is of a core-shell structure, and comprises synthetic Nd<3+>-doped 800nm laser excited nano-particles with a heating effect, an upconversion fluorescence nano-shell layer coating the outer layer, an isolating layer which can be added in the middle depending on the difference of outer layer materials, and a shell layer which is additionally arranged outside an illumination layer to intensify the illumination strength. The method for synthesizing the nano-particles is simple, easy to operate and strong in controllability, and particles with different diameters can be synthesized by controlling the usage of raw materials. The nano-particles synthesized by the method integrate the independent photothermal effect of the material and the upconversion illumination effect, and can be applied to preparation of tumor diagnosis preparations, temperature detection reagents and tumor treatment medicaments, and biological imaging.

The present invention relates to ligands, nanocrystal complexed with the ligands and their use for bio-imaging.

The invention discloses a preparing method and application of fluorescence-imaging copper-and-nitrogen-doped carbon quantum dots with the dual-light-treating effect. The carbon quantum dots prepared with the method can emit blue fluorescence, and meanwhile the photo-thermal effect, the photodynamic treating effect and the fluorescence imaging effect can be achieved. According to the preparing method, ethylenediamine tetraacetic acid disodium salt and copper chloride dihydrate serve as raw materials, and the copper-and-nitrogen-doped carbon quantum dots are prepared. According to the preparing method and application of the fluorescence-imaging copper-and-nitrogen-doped carbon quantum dots with the dual-light-treating effect, the raw materials are low in cost and easy to obtain and prepare, and an obtained product has the good biocompatibility and the good water solubility, can emit the blue fluorescence, has the absorbing effect and the good photothermal conversion efficiency in infrared and near infrared regions, has the photosensitization effect, and can generate reactive oxygen for photodynamic therapy under illuminating of near-infrared light. The copper-and-nitrogen-doped carbon quantum dots prepared with the method have broad application prospects in the photothermal therapy aspect, the photodynamic therapy aspect, the bioimaging aspect and the like.

The invention discloses an organic compound with a long afterglow effect as well as a preparation method and application of the organic compound. The organic compound has a structural formula as follows: the formula is shown in the description; 9-phenylcarbazole with n-pi* is used as a basic unit, and a meta-position and a para-position of phenyl are modified; after modification, different 9-phenylcarbazole derivatives are obtained; a test shows that the fluorescence lifetime of the compound can reach 100ms or more. The compound provided by the invention has the characteristics of simple synthesis method, easiness of being purified, adjustable luminescent spectrum, flexibility, no need of doping inorganic rare-earth materials, cheap price, low toxicity and the like, and can be widely to various fields including biological imaging, disease diagnosis, optical counterfeiting prevention and the like.