The invention discloses a stable nano
graphene oxide under
physiological condition, which is characterized in that: based on the prior finished product, the nano
graphene oxide also contains two types of hydrophilic groups, namely
carboxylic acid functional group and
sulfonic acid functional group, by further chemically modification. Firstly, crystalline
flake graphite is oxidized by adopting a Hummer method; secondly,
graphene oxide is peeled off by an ultrasonic method; thirdly, the graphene oxide is fragmented into
nano size by an ultrasonic probe method; and finally,
carboxylic acid modification is performed on the nanoscale graphene oxide by an immersion
ultrasound method, carboxyl functional groups are introduced,
benzene sulfonation modification is performed on the graphene oxide, and
sulfonic acid groups are introduced on the edge of the graphene oxide. The nano graphene oxide can improve the stability of graphene oxide in
saline solution or
cell culture media, and a powerful guarantee for the medicinal application of nano graphene oxide under
physiological condition is provided. Compared with the
polyethylene glycol modification method, the method has the advantages of reducing manufacture cost and facilitating
mass production.