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Procalcitonin detection kit, and method of measuring content of procalcitonin therewith

A technology of procalcitonin and kits, which is applied in the field of medical testing, can solve the problems of long sample preparation time, labor and time consumption, etc., and achieve the effects of high accuracy, good precision, and improved detection sensitivity

Inactive Publication Date: 2016-08-10
SHANDONG SHENGBAILING MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the sample size is large, the time to prepare the sample is longer than the test time, which consumes manpower and time

Method used

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  • Procalcitonin detection kit, and method of measuring content of procalcitonin therewith
  • Procalcitonin detection kit, and method of measuring content of procalcitonin therewith
  • Procalcitonin detection kit, and method of measuring content of procalcitonin therewith

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specific Embodiment approach

[0035] The specific embodiment of the present invention is described by the following examples:

example 1

[0037] Preparation of procalcitonin assay kit

[0038] Preparation method of reagent 1 (R1): add Procline300 and 4% polyethylene glycol 6000 at a final concentration of 0.1% to 50mmol / L Tris buffer solution (PH=8.0), mix well and dissolve, pass through a 0.22um filter membrane After filtration, reagent R1 was prepared.

[0039] Preparation method of reagent 2 (R2): procalcitonin antibody was entrusted to a professional company to prepare it according to conventional methods. The antibody has a specific immune reaction with procalcitonin, and has no cross-immune reaction with other antigens, which can meet the needs of this experiment.

[0040] Add EADC:Sulfo-NHS):nano latex particles (300nm or 30nm) in the MES buffer solution with pH=6.0, the mass ratio is 1:0.5:0.1, sonicate for 2-3 seconds, mix well, and shake at room temperature Activate on bed for 2 hours, centrifuge at 17000rpm for 30 minutes, wash 3 times with MES buffer of PH=6.0, then suspend the microspheres in HEPE...

example 2

[0043] Preparation of procalcitonin assay kit

[0044] The preparation method of reagent 1 (R1): in 40mmol / L sodium phosphate buffer (equal concentration of NaH 2 PO 4 and Na 2 HPO 4 Preparation, 0.1% sodium azide, 2% polyethylene glycol 2000, 0.5% EDTA.2Na, 0.5% BSA were added to the final concentration of 0.1% (PH=8.0), mixed and dissolved thoroughly, and filtered through a 0.22um filter membrane to prepare Reagent R1.

[0045]Preparation method of reagent 2 (R2): procalcitonin antibody was entrusted to a professional company to prepare it according to conventional methods. The antibody has a specific immune reaction with procalcitonin, and has no cross-immune reaction with other antigens, which can meet the needs of this experiment.

[0046] Add EADC:Sulfo-NHS):nanolatex particles (400nm or 40nm) to the MES buffer solution with pH=6.0, the mass ratio is 1:0.5:0.1, ultrasonic for 3 seconds, mix well, and activate on a rotary shaker at room temperature Centrifuge at 16,...

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Abstract

The invention especially relates to a procalcitonin detection kit, and a method of measuring content of procalcitonin therewith. The procalcitonin detection kit includes a reagent R1 and a reagent R2, wherein the reagent R1 is a reaction liquid formed by mixing various surfactants and used for promoting antibody-antigen specificity reaction, and the reagent R2 is a nano-latex particle buffer liquid of an anti-human procalcitonin antibody and contains 0.1-2% of nano-latex particles marked by the anti-human procalcitonin antibody. The kit is 0.12 ng / ml in detection sensitivity which is higher than analytic sensitivities of other like products. The nano-latex particle-type procalcitonin detection kit is high in sensitivity, good in specificity, high in accuracy and good in precision, wherein latex particles in different particle sizes are crosslinked with a polyclonal antibody to form conjugates in different particle sizes, which are then mixed according to different ratio, so that the kit is increased in linearity scope and is improved in detection sensitivity.

Description

(1) Technical field [0001] The invention belongs to the field of medical testing, and is an in vitro diagnostic reagent for quantitatively detecting the content of procalcitonin (PCT) in human blood or serum, in particular to a procalcitonin assay kit and procalcitonin method for the determination of content. (2) Background technology [0002] At present, the experimental method for detecting PCT includes radioimmunoassay method, which uses artificially synthesized polyclonal antibodies to specifically recognize and link the synthetic amino acid procalcitonin. This method can detect the mixture of free PCT, bound PCT and calcitonin gene-related peptide precursor, but cannot distinguish the above three substances. The detection time of this method is long (19-22 hours), and there is contamination of radioactive elements. [0003] The double-antibody sandwich immunochemiluminescence method uses double monoclonal antibodies, one of which is a calcitonin antibody and the other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803
Inventor 张立民王琳王元略
Owner SHANDONG SHENGBAILING MEDICAL TECH CO LTD
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