Method for tumor marker quantitative determination through metal-wrappage -antibody composite nano particle based on raman enhancement

Abstract

The invention discloses a method for tumor marker quantitative determination through a metal-wrappage -antibody composite nano particle based on raman enhancement. The method comprises the following steps of: dispersing a metal nano particle included in an inorganic substance or an organic substance or a polymer or a precious metal into a buffer solution and adding an antibody to realize reaction,enabling the antibody to wrap the metal nano particle, thereby obtaining the metal-wrappage -antibody composite nano particle, and dispersing the metal-wrappage -antibody composite nano particle in the buffer solution after washing; and repeatedly preparing various metal-wrappage -antibody composite nano particle solution containing different antibodies and having different concentrations, and uniformly mixing the solution; mixing purified liquid containing a tumor marker with the nano particle mixed solution, after reaction, obtaining a new metal-wrappage -antibody-antigen composite nano particle formed after specific binding of the antibody and the antigen, then, performing transmission spectrum detection, and obtaining concentration of various tumor markers. The method provided by theinvention has the advantages of being simple to operate and highly sensitive and having wide detection intervals and strong specificity. The method can simultaneously perform quantitative analysis forvarious tumor markers, and has great significance in early diagnosis of tumor.

Description

technical field [0001] The present invention relates to the field of physics, in particular to optical detection technology, in particular to Raman spectroscopy detection technology, in particular to a method for quantitative detection of tumor markers based on Raman-enhanced metal-encapsulation-antibody composite nanoparticles. Background technique [0002] At present, cancer has seriously threatened human health and has become one of the leading causes of death. The World Cancer Report 2014 released by the World Health Organization (WHO) estimates that the number of cancer deaths worldwide will reach 13 million in 2030. At the same time, the data provided by the World Health Organization also shows that one-third of cancer patients can be cured through early detection and early treatment. For example, the 5-year survival rate of stage I breast cancer, nasopharyngeal cancer and laryngeal cancer is close to 100%. However, the patients found by clinical testing are often in ...

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Problems solved by technology

[0005] The object of the present invention is to provide a method for the quantitative detection of tumor markers based on Raman-enhanced metal-encapsulation-antibody composite nanoparticles, which is based on the Raman-enhanced metal-encapsulation-antibody composite nanoparticles for quantitative detection of tumors The marker method should solve the technical problems of tedious sample processing, easy interference, inability to quantify and inability to perform multi-channel tumor marker measurement on biological samples in the prior art

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