2066 results about "Butyric acid" patented technology

The present invention is directed to microparticles, to microparticle compositions containing the same, to methods of forming the same, and to uses for the same, including use for a vaccine, for raising an immune response, for treatment of a disease and for diagnosis of a disease. The microparticles comprise a biodegradable polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, or a polycyanoacrylate, and a detergent selected from a cationic detergent and an anionic detergent. The microparticles further comprise an antigen adsorbed on the surface of the microparticle.

Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor are used in a medium with gamma amino butyric acid, pipecholic acid, lithium and lipids, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. A humanized matrix of human proteins can be used as a basement matrix to culture the cells. New lines of human embryonic stem cells made using these culture conditions, the medium and the matrix, will never have been exposed to animal cells, animal products, feeder cells or conditioned medium.

Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.

The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.

The invention is a fibrin glue that avoids the use of fibrinogen and thus eliminates the need for premixing and premature clot formation. The fibrin glue of the invention comprises thrombin, thromboplastin and calcium and may have clotting Factors, VII, IX and X, and the like. The invention also comprises a biosealant for use with the fibrin glue without fibrinogen or for use alone. The biosealant is a two component mixture of gelatin / resorcinol and glyoxal / glutaraldehyde / 4-(p-maleimidophenyl) butyric acid. The two components are mixed on use.

A system for optimizing the production performance of a milk producing animal herd is provided. The system comprises milk sampling means, analytical means comprising separate means for analyzing compounds or parameters that in the presence of compounds indicative of the physiological or nutritional condition of the herd member, generates detectable signals, and means for directing a part of the milk sample to each separate analyzing means which is controlled by data for the physiological and nutritional state of a herd member such that the directing means is only activated at pre-selected points in time or at pre-selected time intervals in the production and or lactation cycles. Specific compounds are compounds indicative of mastitis, including beta-N-acetylhexosaminidase (NAGase) E.C. 3.2.1.52 and lactate dehydrogenase (LDH), protein balance, including milk urea nitrogen (MUN) and total protein, ketosis, including acetolactate, beta-hydroxybutyrate, acetone and lipids, fat and state in reproduction cycle, including a steroid or peptide hormone such as progesterone. Furthermore, the system comprises signal detection means for recording and processing the signals, means for data storage and data output means. Additionally there are provided methods for optimizing the production performance of a milk producing animal herd and an apparatus herefor.

Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor are used in a medium with gamma amino butyric acid, pipecholic acid, lithium and lipids, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. A humanized matrix of human proteins can be used as a basement matrix to culture the cells. New lines of human embryonic stem cells made using these culture conditions, the medium and the matrix, will never have been exposed to animal cells, animal products, feeder cells or conditioned medium.

The invention relates to acid solution used for processing the magnesium alloy surface. The acid solution is water solution which contains acids, inhibitor and wetting agent, wherein, the acids are first acids or mixture of first acids and second acids; the first acids are selected from one type or a plurality of types among citric acids, oxalic acids, tartaric acids, methanoic acids, acetic acids, metacetonic acids, butyric acids, glutaric acids, phenylformic acids, benzene dicarboxylic acids, lactic acids, glycolic acids, glyoxylic acids and amino acids; and the second acids are hydrochloric acids and / or nitric acids. By adoption of the acid solution, the magnesium alloy surface can be fully activated; the membranous layer of a converting film which is formed on the magnesium alloy surface after chemical conversion process is compact, has erosion resistance and good binding force with a paint film. Moreover, the method is a environment-friendly method for processing the magnesium alloy surface.

The invention relates to a eubacterium and clostridium preparation and application of the same, in particular to a microecological preparation which is prepared for replenishing butyric acid bacteria and butyric acid produced by intestinal tract by taking single eubacterium, single clostridium or a eubacterium and clostridium composition as a main active composition, and application of the same in treating related diseases through butyric acid production, and belongs to the field of biological medicine.

The invention discloses a preparation method of one of components of a novel antihypertensive drug LCZ696, namely, sacubitril (sacubitril, AHU-377, I). The method comprises the following preparation steps: carrying out cyclization, addition, debenzylation, ring opening, esterification, amidation and the like on a chiral induciton reagent (S)-1-(alpha-aminobenzyl)-2-naphthol (S-betti Base) and 2R-methyl-4-oxo-butyric acid to prepare the sacubitril (I). The preparation method is available in raw materials, concise in process, economic and environmentally friendly, and is suitable for industrial production.

The invention discloses a method for preparing L-glufosinate-ammonium by use of amino acid dehydrogenase. The method comprises the following steps: taking 2-carbonyl-4-(hydroxymethylphosphonyl)butyric acid or a salt thereof as a substrate, taking a cell of the isolated glutamate dehydrogenase or in vitro expression glutamate dehydrogenase as a catalyst to perform reductive amination reaction under the condition of the existence of inorganic amino donor and reduced coenzyme, thereby acquiring the L-glufosinate-ammonium. The method disclosed by the invention is high in raw material conversion rate and high in yield, the product is easy to separate and purify, and the chirality purity is high; compared with the transaminase and like catalysis technology, the process is relatively simple, and the raw material conversion rate reaches up to 100%.

The present invention belongs to the field of food biotechnology and is especioally microbial conversion process of preparing gamma-amino butyric acid. Gamma-amino butyric acid is prepared with lactic acid bacteria or lactic acid bacteria and saccharomycete mixture as bacteria strain, sodium L-glutamate as converting substrate and through fermentating process with fermenting medium with carbon source, nitrogen source and inorganic salt. The selected lactic acid bacteria can growth well in MRS or PYG culture medium at 25-40 deg.c and may be used as culture seed liquid. Of the culture medium, the carbon source is glucose, and the nitrogen source may be one or several selected from corn paste, yeast paste, defatted peanut cake powder, etc. After fermentation, the concentration of gamma-aminoacid may reach as high as 300-500 ml / 100 ml.

The invention discloses a method for producing L-2-aminobutyric acid, and the method comprises the following step: catalyzing L-threonine utilized as a raw material through an enzyme catalysis system consisting of threonine deaminase, L-amino acid dehydrogenase and coenzyme regeneration systems, thus producing the L-2-aminobutyric acid. The method for producing theL-2-aminobutyric acid has the advantages that the raw material is low in price, the property is stable, and the production cost of the L-2-aminobutyric acid can be greatly lowered, the conversion rate and product concentricity are high, no influence caused by byproducts exists, and the method is suitable for industrialization application.

A method for attracting bed bugs includes placing a climb-up pitfall trap apparatus that includes a chemical lure composition. The lure composition includes a combination of (a) L-lactic acid, and (b) at least one fatty acid or salt selected from the group consisting of (1) propionic acid, (2) butyric acid, and (3) valeric acid, and (c) 1-octen-3-ol, and (d) a suitable ketone, and (e) a suitable aliphatic sulfide. The trap may also include in conjunction with the lure composition a carbon dioxide source and / or a heat source to compose a lure arrangement.

The invention provides dilute and concentrated, aqueous, pharmaceutical compositions comprising gamma-hydroxybutyric acid or pharmaceutically acceptable salts thereof; gamma-butyryl lactone; 1,4-butanediol; 4-hydroxyl pentanoic acid or pharmaceutically acceptable salts thereof; 4-hydroxyl pentanoic acid lactone, or combinations thereof, and a coloring agent and / or flavoring agent that is useful in preventing sexual assault or date rape. Methods of treating conditions responsive to the administration of gamma-hydroxyl butyric acid and / or its pharmaceutically acceptable salts; gamma-butyryl lactone; 1,4-butanediol; 4-hydroxyl pentanoic acid and / or its pharmaceutically acceptable salts; and 4-hydroxyl pentanoic acid lactone are also described. The invention provides methods for treating patients with acquired resistance to GABAnergic agents.

The present invention is directed to a compound of 2-N halo-4-methylsulfonyl-butyric acid, such as 2-N chloro-4-methylsulfonyl-butyric acid, or a pharmaceutically acceptable salt or solvate thereof. The present invention is also directed to a pharmaceutical composition comprises the compound and a pharmaceutically acceptable carrier. The present invention is further directed to a method for treating inflammation or inflammatory-related disorders, bacterial infection, pain, or skin conditions, by administering 2-N halo-4-methylsulfonyl-butyric acid to a subject in need thereof.

The present invention relates to butyrate producing bacterial strains related to the species Buty{acute over (η)}coccus pullicaecorum to be used in the prevention and / or treatment of intestinal health problems. The present invention therefore provides methods and compositions that overcome the problems associated with the currently used methods for administering butyric acid in the treatment of intestinal health problems in humans and / or animals.

The invention discloses a nonreactive health powdered suckling pig creep compound feed and a preparation method thereof, wherein the feed is prepared from the following materials: corn, broken rice, puffed corn starch, puffed soybean meal, dehulling soybean meal, fermented soybean meal, white fish meal, flour, plasma protein flour, whey powder, fruit sugar, glucose monohydrate, bean oil, calcium formate, calcium biphosphate, salt, enveloped slow release acidulant, choline chloride, suckling pig compound premix, zinc oxide, yeast cell wall polysaccharides, sodium glutamate, arazyme, lipidase, Clostridium butyricum, essential oils, L-lysine hydrochloride, DL methionine, L-threonine, tryptophan and phytase. Based on the physiological characteristics of suckling pig and by the combination of various materials, the feed is supplementary in nutrition and high in design of amino acid and daily ration of energy without adding antibiotics, and Clostridium butyricum and various essential oils are used for protecting the health; therefore, the feed intake of suckling pig can be increased, the weight growth rate is increased, the diarrhea rate after ablactation is reduced, the intestine health of suckling pig can be improved, the immunity is improved, and the survival rate of suckling pig is increased.

Methods are disclosed for forming heptan-4-one, and, optionally, heptan-4-ol, from fermentable sugars. The sugars are fermented using a bacteria or yeast that predominantly forms butyric acid. The butyric acid is subjected to catalytic ketonization conditions to form heptan-4-one, with concomitant loss of water and carbon dioxide. The heptan-4-one can be subjected to catalytic hydrogenation to form heptan-4-ol, an either of these can be included in gasoline compositions. In one aspect, the fermentable sugars are derived from lignocellulosic materials such as wood products, switchgrass, or agricultural wastes, which are delignified to form lignin, cellulose and hemicellulose. The cellulose and hemicellulose can be depolymerized to form glycose and xylose, either or both of which can be fermented by the bacteria. Thus, the methods described herein can convert biomass to a fuel composition or fuel additive, which can be used in a conventional gasoline engine, unlike traditional fuels such as ethanol or biodiesel.

A process for the conversion of carbohydrates from any of a number of sources into butanol and hexanol for fuel or chemical use is disclosed. The process includes conducting a homoacetogenic fermentation to produce an acetic acid intermediate which is chemically converted to ethanol. The ethanol and a remaining portion of the acetic acid intermediate are used as a substrate in an acidogenic fermentation to produce butyric and caproic acid intermediates which are then chemically converted to butanol and hexanol.

This invention pertains to a method for flavoring an ingestible composition with a flavoring agent in organoleptically purified form, unaccompanied by substances of natural origin present in mango. The flavoring agent may be used in a wide variety of ingestible vehicles such as chewing gum compositions, hard and soft confections, dairy products, beverage products including juice drinks and juice products, green vegetable and chicken products, and the like. The present invention also pertains to an ingestible composition comprising an ingestible vehicle and an organoleptically effective amount of the purified flavoring agent. The present invention also pertains to ethyl 4-(thioacetoxy)butyrate represented by the formula, CH3COS(CH2)3COOCH2CH3.

Fluorinated oligopeptides, especially those having 4,4-difluoro-2-amino butyric acid at the C terminus, may be effective inhibitors of hepatitis C virus NS3 protease. Examples of hexapeptides of the invention, optimized for binding in the S1 specificity pocket of the enzyme, may display IC50s at the sub-micromolar level. Embodiments of tripeptides of the invention, having a keto-acid group at the C-terminus are, likewise, potent inhibitors of NS3 protease.

The invention discloses a production method of L-glufosinate. The method includes the steps that with 2-carbonyl-4-(hydroxyl methyl phosphoryl) butyric acid and salt thereof being a substrate, isolated transaminase or a cell catalysis substrate of in-vitro expression transaminase reacts with an amino donor under the condition that the amino donor exists, so that L-glufosinate is obtained, wherein the amino donor is alanine, and the amino acid sequence of transaminase is shown in SEQ ID NO.1-3. With 2-carbonyl-4-(hydroxyl methyl phosphoryl) butyric acid and salt thereof being the substrate and alanine being the amino donor, the transamination reaction occurs through the specific transaminase catalysis substrate, the substrate can be completely converted into L-glufosinate, and the conversion rate of raw materials is high and can reach 100%.

Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.

The process of biosynthesizing gamma-amino butyric acid includes the following steps: slant agar culture to activate Lactobacillus brevis with the preservation number of CGMCC No. 1306, inoculating to GYP or MRS seed culture medium to culture for 10-30 hr, inoculating to GYP or MRS fermenting culture medium in the amount of 0.5-5 % to stationarily culture at 25-35 deg.c for 48-120 hr to obtain fermented liquid containing thallus and centrifugally separating and collecting thallus; washing thallus with sterilized deionized water, suspending wet thallus in citric acid-disodium hydrogen phosphate buffering system with sodium L-glutamate to react for 1-10 hr, and centrifuging the reacted liquid to obtain gamma-amino butyric acid solution. The present invention has low production cost, mild reaction condition, less environmental pollution, simple process, high production efficiency, and high product purity.

The invention discloses a method for preparing a bacillus subtilis and clostridium butyricum composite bacterial preparation, which is characterized by comprising the following steps: (1) preparing seed culture solution, namely preparing a seed solution containing clostridium butyricum and a seed solution containing bacillus subtilis respectively, wherein the concentration of the bacterial solution is 0.5*10<8> to 1.0*10<8>CFU / ml; (2) preparing a coculture medium; (3) adding the seed solution of the bacillus subtilis into the coculture medium prepared by the step (1) according to a ratio of 2 percent, automatically regulating the pH value to 6.5 to 7.0 by using 10-percent diluted hydrochloric acid or 10-percent sodium hydroxide, introducing gas, stirring, controlling dissolve oxygen content to be 20 to 50 percent, culturing for 18 to 20 hours at 37 DEG C, stopping introducing gas, and continuing to stir and culture for 2 to 4 hours; and (4) adding the seed solution of the clostridium butyricum prepared by the step (1) into the coculutre medium by using a peristaltic pump according a ratio of 2 percent, standing at 35 DEG C and culturing for 24 to 28 hours to obtain the bacillus subtilis and clostridium butyricum composite bacterial preparation. In the invention, a microecological preparation is mixed with feed according to a ratio of 0.5 percent, the feed is directly used to feed livestock, the diarrhea rate and death rate of the livestock are lowered effectively, yield is increased, and medicine administration cost is saved.

Provided herein are salts of (S)-2-amino-3-methyl-butyric acid (2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoquinolin-2-yl ester in amorphous and crystalline forms, and processes of preparation, and pharmaceutical compositions thereof. Also provided are methods of their use for treating, preventing, or ameliorating one or more symptoms of neurological disorders and diseases including hyperkinetic movement disorders or diseases.

The invention relates to method and equipment system for separating and recovering organic oxygen-containing compounds in a Fischer-Tropsch synthesis water phase. The equipment system is integrated by adopting twelve towers including a mixed acid cutting tower, an acetaldehyde rectifying tower, a methanol / ethanol dividing tower, a methanol extraction rectifying tower, a methanol rectifying tower, an acetaldehyde removing tower, an ethanol tower, a propanol concentration extracting tower, a propanol intermittent azeotropic distillation tower, a carboxylic acid extraction tower, a carboxylic acid intermittent rectifying tower and an extraction agent recovering tower matched with the carboxylic acid extraction tower for use. By applying the method and equipment system disclosed by the invention, more than ten kinds of organic oxygen-containing compounds such as acetaldehyde, acetone, methanol, ethanol, normal propyl alcohol, normal butanol, acetic acid, metacetonic acid, butyric acid, butanone and the like can be separated from raw materials; and the products can respectively reach the industrial purity. The method and equipment provided by the invention are economic, effective and reasonable; and according to the method and equipment, efficiency of Fischer-Tropsch synthesis industrial enterprises can be greatly increased, production cost is reduced and goal of clean production can be achieved.

The invention relates to a hydroxylated polyurethane water dispersoid for a waterborne soft-feel coating and a preparation method thereof. The hydroxylated polyurethane water dispersoid is the water dispersoid formed by the following steps of: taking the raw materials in parts by weight: 15 to 20 parts of polycarbonate diols, 15 to 25 parts of elastic 4-butanediol, 1 to 5 parts of pentaerythritol, 2 to 5 parts of hydroxylated dimethylolpropionic acid or dihydroxymethyl butyric acid, 10 to 20 parts of fatty group or alicyclic group isocyanates monomers and 0.1 to 1.0 part of catalysts and solvents; carrying out heating polymerization to obtain hydroxylated urethane resin; and then neutralizing a water dispersoid formed after salt forming, self emulsifying and the solvent removing, wherein the coating-4 viscosity change of the coating stored for 6 months is smaller than 5 percent. After the hydroxylated polyurethane water dispersoid prepared by the method is prepared into a double-ingredient waterborne soft-feel coating, better balance is obtained between the coating film soft feel and the coating film resistance on alcohol and contaminants, and the effect of the hydroxylated polyurethane water dispersoid is proved.

A construct that comprises a base material and a polyhydroxyalkanoate, wherein at least a part of the base material is coated with the polyhydroxyalkanoate, and the polyhydroxyalkanoate comprises a 3-hydroxyalkanoic acid unit other than 3-hydroxypropionic acid unit, 3-hydroxy-n-butyric acid unit, and 3-hydroxy-n-valeric acid unit.In addition, an electrostatic charge image developing toner allows to design the toner characteristics such as chargeability, flowability, stability in time and environmental stability uniform among the toners of different colors. The toner has a small particle size enough for enabling uniform dispersion and being excellent in color saturation and transparency. The toner also shows higher contribution to the environmental security. The toner includes a coloring agent of which at least a part of the surface is covered with polyhydroxyalkanoate (PHA). The toner is produced by dispersing the coloring agent in aqueous medium, then fixing PHA synthesizing enzyme to the coloring agent dispersed in the aqueous medium, then adding 3-hydroxyacyl CoA, and executing a PHA synthesizing reaction to cover at least a part of the surface of the coloring agent with PHA. The toner thus obtained is used for an image forming method.