199results about How to "Fully activated" patented technology

The invention relates to a method used for in vitro proliferation of NK cells, and more specifically relates to a method used for realizing large-scaled proliferation of NK cells via autologous peripheral blood mononuclear cell in vitro cultivating. According to the method, proliferation and activation of NK cells are realized via different cytokine combination. The method comprises following steps: autologous peripheral blood mononuclear cells are inoculated in CD3 antibody and CD16 antibody pre-coated culture bottles, and are subjected to stimulation for 16 to 24h; and a serum-free medium containing IL-2 and IL-18 is added. According to the method, CD3 antibody and CD16 antibody are immobilized via coating, direct stimulating activation of NK cells is performed, so that toxicity of NK cells is increased greatly, activation and proliferation of NK cells are realized using two cytokines IL-2 and IL-18, proliferation multiple and cytotoxicity of NK cells are ensured, and culturing cost of NK cells is reduced greatly.

The invention discloses a composite sludge conditioner based on iron-containing sludge pyrolysis residue as well as preparation and application of the composite sludge conditioner. The composite sludge conditioner contains the iron-containing sludge pyrolysis residue and an oxidant matched with the iron-containing sludge pyrolysis residue for use, wherein the iron-containing sludge pyrolysis residue is the pyrolysis residue prepared by the steps of dehydrating iron-containing sludge obtained from an advanced oxidation technique involved with an iron-containing element reagent so as to obtain an iron-containing mud cake, and carrying out pyrolysis on the iron-containing mud cake. Compared with the prior art, the composite sludge conditioner has the advantages that by utilizing an integral reutilization manner of a subsequent treatment processes of the mud cake of the iron-containing sludge and improving the specific reaction condition parameters in each subsequent treatment process step, the treatment problem of the mud cake at the tail end in an existing sludge treatment technique can be effectively solved, and the composite sludge conditioner formed by the mud cake of the iron-containing sludge for the deep dehydration of the sludge can be used as a sludge conditioner for circularly conditioning the sludge, so that the adequate utilization of resources can be realized.