The invention provides novel antimicrobial peptide MP1106 (Seq ID No.1) designed based on Plectasin, and provides a high-efficiency expression of the novel antimicrobial peptide MP1106 (Seq ID No.1) in pichia pastoris. Through the preference of yeast codon for optimization of antibacterial peptide MP1106 encoding gene and construction of recombinant plasmid, the recombinant plasmid is transformed into the pichia pastoris, secretory expression of high density fermentation protein in a 5 L fermentation tank can be achieved, the total protein level reaches 2.134g / L, and purified recombinant MP1106 yield reaches 831mg / L. In addition, recombinant MP1106 cation-exchange chromatography one-step purification method is established, and novel antimicrobial peptide MP1106 in-vitro properties show that the novel antimicrobial peptide MP1106 has strong inhibitory activity against staphylococcus aureus, is free of cell toxicity and stable in high temperature, alkaline environment, serum incubation conditions, and is resistant to papain, pepsase and the like. The novel antimicrobial peptide MP1106 is superior in properties, can realize large-scale industrial production, and is potential to be developed into a novel anti staphylococcus aureus preparation.