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Graphene quantum dot nuclear targeting medicine carrying system as well as preparation method and application thereof

A graphene quantum dot and drug-loading technology, which is applied in the field of biomedicine, can solve the problem that anticancer drugs cannot effectively accumulate in the nucleus, and achieve the effects of enhancing drug cytotoxicity, drug-loaded drug cytotoxicity, and increasing toxicity

Active Publication Date: 2013-12-11
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first purpose of the present invention is to provide a graphene quantum dot core-targeted drug delivery system for the problem that anticancer drugs cannot effectively accumulate in the nucleus where the drug acts in most unmodified nano drug delivery systems preparation method

Method used

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  • Graphene quantum dot nuclear targeting medicine carrying system as well as preparation method and application thereof
  • Graphene quantum dot nuclear targeting medicine carrying system as well as preparation method and application thereof
  • Graphene quantum dot nuclear targeting medicine carrying system as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Graphene Quantum Dot Targeted Drug Delivery System Loading Doxorubicin into Human Breast Cancer Cell MCF-7

[0029] 1. Cytotoxicity of the drug-loaded system:

[0030] The first step is to filter the 0.5 mg / mL graphene quantum dot aqueous solution and the 10 mM doxorubicin aqueous solution with a 0.22 μm filter membrane.

[0031] In the second step, the two reagents are shaken and mixed at room temperature to obtain a drug-loading system solution of doxorubicin with a final concentration of 10 μM and graphene quantum dots with increasing concentrations in turn.

[0032] Step 3: Inoculate MCF-7 cells on a 96-well cell culture plate at a density of 4,000-5,000 cells per well at 37°C, 5% CO 2 , after culturing for 12 hours under saturated humidity conditions to make it adhere to the wall, the medium was removed and washed with 0.1M PBS.

[0033] In the fourth step, 100 μL of drug-loading system solutions with different concentration ratios were added to the 96...

Embodiment 2

[0042] Example 2. Graphene Quantum Dot Targeted Drug Delivery System Loading Doxorubicin into Human Gastric Cancer Cell MGC-803

[0043] The first step is to filter the 0.5 mg / mL graphene quantum dot aqueous solution and the 10 mM doxorubicin aqueous solution with a 0.22 μm filter membrane.

[0044] In the second step, the two reagents are shaken and mixed at room temperature to obtain a drug-loading system solution of doxorubicin with a final concentration of 2 μM and graphene quantum dots with successively increasing concentrations.

[0045] Step 3: Inoculate MGC-803 cells on a 96-well cell culture plate at a density of 4000-5000 cells per well at 37°C, 5% CO 2 , after culturing for 12 hours under saturated humidity conditions to make it adhere to the wall, the medium was removed and washed with 0.1M PBS.

[0046] In the fourth step, 100 μL of drug-loading system solutions with different concentration ratios were added to the 96-well plate, and co-incubated with the cells f...

Embodiment 3

[0048] Example 3. Graphene Quantum Dot Targeted Drug Delivery System Loading Doxorubicin into Drug-resistant Human Breast Cancer Cell MCF-7 (MCF-7 / ADR)

[0049] The first step is to filter the 0.5 mg / mL graphene quantum dot aqueous solution and the 10 mM doxorubicin aqueous solution with a 0.22 μm filter membrane.

[0050] In the second step, the two reagents were shaken and mixed at room temperature for 30 minutes to obtain a drug-loading system solution of doxorubicin and 15 μg / mL graphene quantum dots with a final concentration of 1 μM, wherein doxorubicin and graphene quantum dots The concentration ratio is 1:15.

[0051] Step 3: Inoculate MCF-7 cells on 24-well cell culture plates with a density of 5×10 per well. 4 cells, among them, a Φ14mm gelatin-coated coverslip was placed in a 24-well plate in advance, and the cells were placed at 37°C, 5% CO 2 , after culturing for 12 hours under saturated humidity conditions to make it adhere to the wall, the medium was removed a...

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Abstract

The invention discloses a graphene quantum dot nuclear targeting medicine carrying system as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, sterilizing an aqoeous solution of a graphene quantum dot and an aqoeous solution of an anti-cancer medicine; secondly, mixing the aqoeous solution of the graphene quantum dot and the aqoeous solution of the anti-cancer medicine according to the mass ratio of (5:1)-(50:1) to obtain a medicine carrying system; thirdly, co-cultivating the medicine carrying system and cells, detecting the toxicity of the cells by using an MTT (Methyl Thiazolyl Tetrazolium) method and detecting a medicine loaded into the cells by using a fluorescent microscope. According to the invention, by virtue of the characteristic that the graphene quantum dot has a single-atom planar structure, the graphene quantum dot and a micromolecule anticancer medicine with a polycyclic planar structure are combined by bonds to form the medicine carrying system which can stably exist in the aqoeous solution. The graphene quantum dot has a medicine carrying function; meanwhile, the graphene quantum dot has a special structure, so that the toxicity of the medicine to the cells can be increased. The medicine carrying system has the advantages of lower toxicity, simple preparation method, easiness for implementation and double functions of carrying the medicine and enhancing the toxicity of the medicine to the cells.

Description

technical field [0001] The invention relates to the technical field of biomedicine, more specifically, to a graphene quantum dot core targeted drug loading system, a preparation method of the system and an application in anticancer drugs. Background technique [0002] Nanomaterials have shown unprecedented development prospects in terms of improving drug intake, targeting, and efficacy due to their nanoscale structural features and diverse properties. A variety of nanomaterials, such as carbon nanotubes, nanopolymers, nanoparticles, etc., are being extensively developed for cancer therapy. However, many nano-loaded drugs only improve the efficiency of drug entry into cells, but do not improve the efficiency of drug entry into the nucleus, especially the drug resistance of drug-resistant cells has not been effectively improved. Therefore, whether the nano-drug delivery system can effectively deliver anticancer drugs to the nucleus is still an urgent problem to be solved. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K31/704A61K31/136A61K31/4745A61P35/00A61K47/52
Inventor 张井岩王翀
Owner EAST CHINA UNIV OF SCI & TECH
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