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DOTAP-mPEG-PLA nanoparticles, solution of nanoparticles, medicine-carrying compound as well as preparation method and application

A nanoparticle and composite technology, applied in the field of medicine, can solve the problems of low transfection efficiency and difficulty, and achieve the effect of low cytotoxicity and high gene transfection rate

Active Publication Date: 2016-03-02
SICHUAN UNIV
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  • Description
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Problems solved by technology

When PEG-PLA nanoparticles are used in the gene transfer system, it is difficult to load genes with mPEG-PLA nanoparticles alone, and the transfection efficiency is low

Method used

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  • DOTAP-mPEG-PLA nanoparticles, solution of nanoparticles, medicine-carrying compound as well as preparation method and application
  • DOTAP-mPEG-PLA nanoparticles, solution of nanoparticles, medicine-carrying compound as well as preparation method and application
  • DOTAP-mPEG-PLA nanoparticles, solution of nanoparticles, medicine-carrying compound as well as preparation method and application

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Embodiment Construction

[0042] The above-mentioned content of the present invention will be further described in detail below through specific implementation in the form of examples, to illustrate but not limit the present invention.

[0043] The molecular structure formulas of mPEG-PLA and DOTAP are shown in figure 1 , DPP nanoparticles are prepared by mPEG-PLA and DOTAP self-assembly method, the structure diagram is shown in figure 2 .

[0044] 1. Experimental method

[0045] 1.1 Preparation method of DPP nanoparticles and VSVMP / DPP composite

[0046] 1.1.1 Preparation of DPP nanoparticles

[0047] DPP nanoparticles are prepared by a self-assembly method: first, mPEG-PLA copolymer (45mg) and DOTAP (5mg) are dissolved in dichloromethane solution respectively, and then transferred to a flask for mixing; then connected to a rotary evaporator, the The mixed solution was placed in a water bath at 60° C., and rotated under vacuum for 30 minutes to completely evaporate the methylene chloride and form...

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Abstract

The invention belongs to the field of medicines, and in particular relates to DOTAP-mPEG-PLA nanoparticles, a solution of the nanoparticles, a medicine-carrying compound as well as a preparation method and an application. The invention aims at providing an amphiphilic mPEG-PLA diblock copolymer modified by DOTAP, and a novel biodegradable gene carrier, namely, the DOTAP-mPEG-PLA nanoparticles (DPP nanoparticles for short) is prepared through a self-assembly method. The DPP nanoparticles have good DNA binding capacity, gene plasmids can be effectively guided into cancer cells, and the advantages of high transfection efficiency and low cell toxicity are achieved.

Description

technical field [0001] The invention belongs to the field of medicine, and specifically relates to DOTAP-mPEG-PLA nanoparticles, nanoparticle solutions, drug-loaded complexes, preparation methods and applications thereof. Background technique [0002] The gene transfer system has important applications in gene function research and gene therapy. Currently used gene transfer systems mainly include two categories: viral vectors and non-viral vectors. Viral vectors include retroviral vectors, lentiviral vectors, adenoviral vectors and adenoviral-associated vectors, which can efficiently deliver therapeutic genes into cells, but their large-scale production is difficult, and the gene capacity that can be delivered is small, which can easily cause immune reactions , a potential biosafety risk. Non-viral gene carriers include liposomes, cationic nanoparticles, inorganic nanoparticle carriers, etc., which have the characteristics of low immunogenicity, good safety, and easy large...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/34A61K48/00A61P35/00
Inventor 苟马玲魏于全门可
Owner SICHUAN UNIV
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