Eubacterium, Clostridium preparation and use thereof
A technology of Eubacterium and Eubacterium rectum, applied in the field of biopharmaceuticals, can solve the problems such as the inability to produce butyric acid, the unsatisfactory curative effect of the disease, and the impact on the curative effect.
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preparation Embodiment 1
[0063] Medicine Preparation Example 1 Preparation of Oral Rectal Live Bacteria Powder and Cylindrical Live Bacteria Powder
[0064] 1 Preparation of bacterial powder and identification of strains
[0065] Take healthy baby feces, then put the feces in a sterilized anaerobic bottle, blow in nitrogen gas and mix well at the same time, quickly take 2 grams of feces from it and add it to 18mL sterilized diluent, blow in nitrogen gas and mix well at the same time. In the console, do 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7 Three dilution gradients, spread on the Eubacterium selective single colony isolation solid medium, place in an anaerobic tank, anaerobic culture at 37°C for 48 hours, select two single colonies with good growth and inoculate them into the Eubacterium liquid respectively The expansion medium was placed in an anaerobic tank, and the expansion culture was carried out at 37° C. for 48 hours in anaerob...
preparation Embodiment 2
[0088] Preparation of Oral Clostridium tertiary Live Bacteria Powder and Clostridium Butyricum Live Bacteria Powder Intestinal Residents
[0089] 1 Preparation of bacterial powder and identification of strains
[0090] Take healthy baby feces, then put the feces in a sterilized anaerobic bottle, blow in nitrogen gas and mix well at the same time, quickly take 2 grams of feces from it and add it to 18mL sterilized diluent, blow in nitrogen gas and mix well at the same time. In the console, do 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7 Three dilution gradients, 80°C water bath for 13 minutes, spread on the solid medium for Clostridium selective single colony isolation, place in an anaerobic tank, culture at 37°C for 48 hours, and select two single colonies with good growth They were respectively inoculated into Clostridium liquid expansion medium, placed in an anaerobic tank, and amplified at 37° C. for 48 hours und...
Embodiment 1
[0112] Example 1. Research on the repairing effect of Eubacterium and Clostridium on intestinal mucosa
[0113] 1 Materials and methods
[0114] 1.1 Materials
[0115] 1.1.1 Drug Rectal Fungal Liquid (10 7 CFU / mL); columnar fungal liquid (10 7 CFU / mL); the third Clostridium solution (10 7 CFU / mL); Intestinal resident Clostridium butyricum liquid (10 7 CFU / mL); Bifidobacteria liquid (10 7 CFU / mL); dextran sodium sulfate (DSS, 10g / bottle, batch number: BI03071, Sino-American Biotec); Alcian blue (1g / bottle, batch number: BS0084, Sino-American Biotec).
[0116] 1.1.2 Animals 70 SD male rats, weighing 80-100 g, standard grade 2, were purchased from the Experimental Animal Center of China Institute for the Control of Pharmaceutical and Biological Products, license number: SCXK (Beijing) 2005-0010.
[0117] 1.2 Method
[0118] 1.2.1 Modeling and treatment of intestinal mucosal injury
[0119] 80 rats, 10 rats were used as the normal control group, and the other 70 rats were ad...
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