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439 results about "Milk sample" patented technology

System for optimizing the production performance of a milk producing animal herd

A system for optimising the production performance of a milk producing animal herd is provided. The system comprises a milk sampling apparatus, an analytical apparatus comprising separate equipment for analyzing compounds or parameters that in the presence of compounds indicative of the physiological or nutritional condition of the herd member, generates detectable signals, and apparatus directing a part of the milk sample (directing apparatus) to each separate equipment, which is controlled by data for the physiological and nutritional state of a herd member, so that the directing apparatus is only activated at pre-selected points in time or at pre-selected time intervals in the production and / or lactation cycles. Specific compounds are compounds indicative of mastitis, including beta-N-acetylhexosaminidase (NAGase) E.C. 3.2.1.52 and lactate dehydrogenase (LDH), protein balance, including milk urea nitrogen (MUN) and total protein, ketosis, including acetolactate, beta-hydroxybutyrate, acetone and lipids, fat and state in reproduction cycle, including a steroid or peptide hormone, e.g., progesterone. Furthermore, the system comprises equipment for signal detection to record and process the signals, equipment to store data and equipment to produce data output. Methods for optimizing the production performance of a milk producing animal herd and an apparatus therefor.
Owner:LATTEC

Apparatus and method for obtaining rapid creamatocrit and caloric content values of milk

The invention relates to a centrifuge apparatus with a built-in sample tube reader and methods for rapidly obtaining measurements of creamatocrit, fat content and / or energy (caloric) content from low-volume fresh and frozen milk specimens.
Owner:SEPARATIONS TECH

Quantitative detection method for bovine alpha-lactalbumin

The invention relates to a quantitative detection method for thermal-denaturation and non-denaturation bovine alpha-lactalbumin in milk and milk products by applying an enzymolysis-liquid chromatography and mass spectrometry combination technology. The quantitative detection method comprises the steps as follows: taking a certain amount of milk or milk samples, dissolving and diluting the milk or milk samples in water to obtain solution with total protein content being about 1mg / mL; after volume metering, correctly sucking 500 mu L, adding an internal standard substance, reacting disulfide bond with dithiothreitol (DTT), alkylating to protect sulfydryl produced in reaction by iodoacetamide (IAA), and then conducting constant-temperature and constant-time enzymolysis with trypsin; and separating enzymolysis products by reversed phase liquid chromatography, conducting detection with a mass spectrum multiple reaction monitoring (MRM) manner, and calculating the result by an internal standard method. The quantitation limit of the method is 0.001g / 100g; when adding amount is 0.2, 1.7 and 5.0g / 100g, the recovery rate is 98.9-110.8% (n is equal to 6) and repeatability: RSD (Relative Standard Deviation) is smaller than 7.6%; and the quantitative detection method can be applicable to the quantitative detection of samples with different contents of bovine alpha-lactalbumin.
Owner:ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION

Automatic counting instrument for body cell of milk / bacteria through direct microscopic method

This invention provides a direct microscopic examination method milk somatic cells/bacteria automatic counter. The automatic counter comprises: a sampling/smearing apparatus, a dyeing apparatus, a washing/drying apparatus, a microscopic detecting apparatus, a convey apparatus for conveying the milk sample as well as glass slide to and out of the sampling/smearing zone, and another convey apparatus for conveying the smeared glass slide to the dyeing zone, washing/drying zone and microscopic detecting zone, which comprises multi-stage glass slide convey mechanisms and glass slide running mechanisms. The automatic analyzer comprises a smearing apparatus, a dyeing apparatus, a washing/drying apparatus a microscopic detecting apparatus and a control apparatus for convey apparatuses. This invention divides direct microscopic examination method milk somatic cells/bacteria analysis and assay into multiple mechanical actions performed by different apparatuses, and sets corresponding working positions and working zones. The working positions and working zones are connected via the convey apparatuses, and can reasonably and effectively perform automatic detection under the control of the control apparatus. The automatic counter has high counting precision and repeat precision, and the working efficiency is largely increased.
Owner:HANGZHOU ULTRASUN TECH

Ultrasensitive fluorescence response method for detecting melamine in milk

The invention relates to a method for detecting melamine in milk which is built based on the inner filter effect (IFE) between cysteine (L-Cys)-modified CdS quantum dots and citric acid ligand coating gold nanoparticles (AuNPs), and belongs to the field of analytical chemistry. The method comprises the detecting steps of preparing the AuNPs, synthesizing and purifying the L-Cys-modified CdS quantum dots, pre-treating a milk sample and detecting the melamine in the milk. According to the principle of recovering the fluorescence intensity of the CdS quantum dots by adopting the AuNPs to cause the fluorescence quenching of the CdS quantum dots and adopting the melamine to induce the aggregation of the AuNPs, the method can simply, quickly and sensitively detect the melamine in the milk so as to provide convenience to future dairy industry.
Owner:JILIN UNIV

Zoonosis tuberculosis fluorescence PCR rapid diagnosis kit

The invention discloses a fluorescence PCR quick diagnosis reagent box of the zoonosis tuberculosis, which belongs to the biological technology field. A group of nucleotide sequence for detecting the zoonosis tuberculosis is the nucleotide sequence shown from the sequence table SEQ ID No.1 to the sequence table SEQ ID No.12. The content disclosed by the invention comprises a fluorescence PCR primer and a probe sequence for a mycobacterium tuberculosis composite group, mycobacterium tuberculosis, cow mycobacterium and fowl mycobacterium, a fluorescence PCR reaction system, a detection program, a reaction parameter and a judging method of various reagent boxes, and the preliminary treatment and the nucleic acid extraction method of various clinic samples of bacteria fluid, milk sample, blood sample, sputum, fecal sample and lymph nodes. The invention provides diagnosis reagent for the zoonosis tuberculosis diagnosis of which the technology method is special, sensitive and quick and the quality is reliable. The adoption of the reagent box provided by the invention can accomplish the whole process from the sample treatment to the fluorescence PCR detection within a day, and the detection sensitivity can reach the single gene copy or the signal bacterial cell.
Owner:INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU +1

Method for rapidly testing nitrite in fresh milk

The invention provides a method for rapidly testing nitrite in fresh milk. The method comprises the following steps: adding 3-6ml of milk sample in a colorimetric tube, then adding 1-2ml of 20% tartaric acid solution and 1-2ml of 0.5% sulfanilic acid solution in turn, shaking evenly, adding 1-2ml of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution, wherein the lower dosage of 20% tartaric acid solution is 1ml, the lower dosage of 0.5% sulfanilic acid solution is 1ml, and the lower dosage of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution is 1ml; and observing after fully shaking, wherein if different shades of pink appears in the colorimetric tube, the sample is proved to contain nitrite. The size of the selected colorimetric tube with a stopper is 15-20ml. The lower limit detection value of the method is 0.1mu g / ml; and the effects of the method are that the dosage is small, the preservation time is long and the detection sensitivity is high. The reagent kit prepared by the method is more convenient to use.
Owner:XINJIANG AGRI UNIV

Milk sampling apparatus and method

The present invention refers to a milk sampling apparatus for use with an automated milking system, said apparatus comprising a cassette (7) wherein milk sample collecting elements (9) are placed, and at least one filling member (27) capable of being placed above a selected one of said milk sample collecting elements (9) by means of a positioning system, and capable of bringing a milk sample, representatively taken from milk yielded during the milking of an animal by means of said automated milking system, into said selected one of said milk sample collecting elements (9). According to the invention the milk sampling apparatus comprises agitating means, preferably a shaking table (5), capable of agitating said milk sample.
Owner:DELAVAL HLDG AB

High-throughput screening method for antibacterial drugs in milk

The invention belongs to the technical field of antibacterial drug residue detection in foods, particularly relates to a high-throughput screening method for antibacterial drugs in milk and relates to a method for detecting 120 antibacterial drug residues in milk at the same time. The detection method comprises the steps of (1) building a technology for pretreatment of antibacterial drugs in milk; (2) building a method for detecting antibacterial drugs in milk; (3) building a blank milk spectral library; (4) building a milk antibacterial drug residue spectral library; and (5) comparing the blank milk spectral library with the milk antibacterial drug residue spectral library to obtain the variety and the content of antibacterial drugs in a to-be-detected milk sample. The method for detecting antibacterial drug residues in a milk sample is simple and fast in operation, low in organic reagent dose and high in sensitivity. The variety and the content of antibacterial drugs in a milk sample can be quickly and accurately identified and the method is suitable for monitoring antibacterial drug residues in milk.
Owner:HUAZHONG AGRI UNIV

Purification method for lactoferrin and lactoperoxidase

The invention provides a purification method for recombination human lactoferrin, animal lactoferrin and lactoperoxidase. The method comprises: taking a milk sample containing recombination human lactoferrin as a raw material, pre-treating to obtain degreased milk, performing cation exchange chromatographic separation on the obtained degreased milk, then under different salt concentration conditions, performing elution separation on lactoferrin and lactoperoxidase, so as to obtain high-purity lactoferrin and lactoperoxidase, and further performing separation on the solution rich in lactoferrin by utilizing hydrophobic interaction chromatography to respectively obtain recombination human lactoferrin and animal source lactoferrin both with the purity of 99% or more. The purity of recombination human lactoferrin obtained by utilizing the purification method provided by the invention is 99% or more, and also recombination human lactoferrin has the physical and chemical activities and physiological functions all similar to those of natural human lactoferrin; and the technology is a one-step purification process, so that the purification method is low in cost, simple and suitable for industrialized large-scale production.
Owner:北京济普霖生物技术有限公司 +1

Rapid detection method of milk protein content based on dielectric spectrum technique

The invention discloses a rapid detection method of the milk protein content based on a dielectric spectrum technique. According to the rapid detection method, dielectric spectra (a relative dielectric constant spectrum and a dielectric loss factor spectrum) of a batch of milk samples within a radio frequency/microwave range are obtained by utilizing a network analyzer (or an impedance analyzer) and a coaxial probe, characteristic dielectric variables which express the milk protein content are extracted from the dielectric spectra, linear or nonlinear prediction models which are used for predicting the milk protein content are established on the basis of the dielectric spectra or the extracted characteristic dielectric variables within the whole frequency range, the models are inspected, and the model of which the root-mean-square error is the minimum is used as the optimum model for predicting the milk protein content. Data of the dielectric spectra under unknown characteristic frequency of the milk samples are substituted into the optimum model, so that the protein content of the samples can be calculated. The rapid detection method has the advantages of rapidity in detection, convenience, high precision, simplicity in operation and capability of real-time online detection.
Owner:NORTHWEST A & F UNIV

Quantitative detecting method for estrogen in milk

The invention provides a quantitative detecting method for estrogen in milk. The quantitative detecting method includes steps of 1) performing derivatization pretreatment for a milk sample; and 2) measuring the content of the estrogen in the milk sample by the aid of online solid-phase extraction and two-dimensional liquid chromatogram tandem mass spectrum analysis. Owning to sample derivatization measurement, detection sensitivity is greatly improved. A two-dimensional liquid chromatogram tandem mass spectrum system is used for detecting, accordingly, online automatic purification of the sample is realized, and the detection efficiency of the sample is greatly improved. In addition, the analysis method is fine in recovery rate, high in automation degree and simple and easy in operation, and can fast and synchronously analyze the content of various types of trace estrogen in the sample.
Owner:INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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