Method for detecting tripolycyanamide in milk based on surface-enhanced Raman activity chip

Abstract

The invention relates to a method for detecting tripolycyanamide in milk based on a surface-enhanced Raman activity chip, and belongs to the technical field of detection of additives in foods. The method comprises the following steps: pre-processing a milk sample containing the tripolycyanamide; preparing a substrate with a surface-enhanced Roman activity for detecting a sample to be detected; processing the substrate with the surface-enhanced Roman activity by the sample to be detected; collecting surface-enhanced Raman signals of the tripolycyanamide in the sample to be detected through a Raman spectrograph. According to the special surface-enhanced Raman signals of the tripolycyanamide, the detection of the tripolycyanamide in the milk sample is achieved. Through the special surface-enhanced Raman signals of the tripolycyanamide, the tripolycyanamide is directly distinguished from other molecules in a milk system, so that the content of the tripolycyanamide in the milk is detected. The detection limit of the method accords with the national standard of the tripolycyanamide in the milk, and a quick and sensitive detection method can be provided for the detection on the tripolycyanamide in the milk.

Description

technical field [0001] The invention belongs to the technical field of additive detection in food, and in particular relates to a method for detecting melamine molecules in milk based on a surface-enhanced Raman active chip. Background technique [0002] The melamine molecule (1,3,5-triazine-2,4,6-triamine) is a raw material used in the manufacture of melamine resin. In recent years, criminals have illegally added melamine molecules to protein foods such as milk, the main purpose of which is to increase the false protein content of the product. The reason is that the molecular nitrogen content of melamine is as high as 66%, and the conventional method of detecting the protein content in food is to determine the protein content according to the nitrogen content in the product, such as the Kjeldahl nitrogen method, which cannot distinguish protein from melamine molecules . Therefore, criminals achieve the purpose of falsely increasing the protein content of products by illeg...

AI Technical Summary

Problems solved by technology

This kind of method is not only complicated to operate, but also has high requirements on the quality of the personnel who operate the instrument and the performance of the instrument itself.

And detection time-consuming, high operating costs

Due to the limitations of the instruments used, rapid on-site detection and real-time monitoring during food production cannot be completed

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