42 results about "Zoonosis" patented technology

The invention discloses a polymerized peptide and a gel having a collagen-like structure. Although collagen is useful as a medicinal material, its use in humans involves the risks of allergy and zoonoses. Therefore, artificial collagens and the like are being studied as medicinal materials. However, typical artificial collagens cannot be considered completely sufficient, given their lack of physical strength, being denatured by heat, inability to be processed into a sheet, and the like, and thus being problematic in terms of practicality. In the present invention, a collagen-like peptide having a triple helical structure comprising triple strands of peptide chains including a plurality of cysteine (cys) residues is oxidized and cross-linked to prepare a polymerized peptide. This polymerized peptide may be processed into and used as a hydrogel and a thin-film sheet. In addition, by inserting a functional amino acid sequence present in biomacromolecules, particular physiological functions such as cell adhesiveness may be obtained. Further, the present invention provides: methods for preparing the polymerized peptide and the like; and uses thereof as compositions in cell cultures, wound coverings, regenerative medicinal materials, and research materials and the like by using the same.

The invention discloses a TaqMan probe for real-time fluorescence PCR (Polymerase Chain Reaction) detection of angiostrongylus cantonensis infected snails on the basis of a modern molecular biological technology, which is a detection method applied to snails infected with angiostrongylus cantonensis. The angiostrongylus cantonensis is food-borne zoonosis and is infected mainly through eating infected snails by mistake. The invention aims to provide a rapid and accurate method for detecting the snails infected with the angiostrongylus cantonensis. The TaqMan probe is technically characterized in that (1) a primer(upstream) having the sequence of 5'-TGA TGC TTG TGC GGT TTT C-3', a primer- (downstream) having the sequence of 5'-CCGAAG GCA AAG CAC ACA C-3' and a probe having the sequence of 5'-CGC TAT ACA CGC ACA TTT GCC GG-3' are provided; (2) the total volume of a reaction system is 20 mu L, wherein the reaction system comprises 10 muL of 2*Taq PCR MasterMix, 1 muL of Taqman probe, 1 muL of the upstream primer, 1 muL of the downstream primer, 1 mu L of template and the balance of ddH2O; and (3) in a reaction process, the pre-degeneration time is 3min at 94 DEG C, 10s at 94 DEG C and 30s at 60 DEG C, and the circulation is carried out for 40 times. The invention provides a new technological measure for food quarantine as well as investigation and monitoring of epidemic situations and has far reaching importance on preventing and controlling the occurrence of the angiostrongylus cantonensis diseases.

The invention discloses a gene chip for detecting six viruses of zoonosis-borne diseases and the application thereof. The gene chip for the viruses of the zoonosis-borne diseases is a gene chip for detecting at least one virus in six viruses of hepatitis E virus, Nipah virus, rabies virus, Rift Valley fever virus, foot and mouth disease virus and Vesicular stomatitis virus, and is fixed with a hepatitis E virus detection probe, a Nipah virus detection probe, a rabies virus detection probe, a Rift Valley fever virus detection probe, a foot and mouth disease virus detection probe and a Vesicular stomatitis virusdetection probe. The invention also discloses a method for detecting the Rift Valley fever virus, the Nipah virus, the foot and mouth disease virus, the Vesicular stomatitis virus, the hepatitis E virus and / or the rabies virus. The invention can stably and specifically implement generic detection of the six viruses of the zoonosis-borne diseases, has the advantages of simpleness, convenience, quickness, good specificity and high sensitivity, and can be used for detecting clinical samples.