42 results about "Zoonosis" patented technology

A green long-acting zoonosis vector control medicament provided by the invention is prepared from an insect growth regulator (IGR), a plant essential oil and / or a low-toxicity high-efficiency pesticide, a bactericide, an insect attractant and / or a repellant, a sustained release agent and the like. According to different use conditions and environments, by the adoption of microemulsification and sol technologies, a spraying agent, a sol coating, a film-forming inhibitor and a sol-forming inhibitor are prepared to form different release mechanisms so as to satisfy control demands in different places. The medicament provided by the invention has low toxicity and high efficiency, has effects of killing ovum, inhibiting insect growth and resisting bacteria and mildew, has a long persistent period, is not easy to generate resistance, has low residual, can be used to minimize the spread of diseases and eliminate the pollution caused by unbalance of drug application, is beneficial to the ecological environment, and is used to control zoonosis vectors (such as flea, louse, mite, tick, mosquito, fly, ant, cockroach and the like) in the fields of public place, garden, urban landscaping, means of transportation, neighborhood, hotel, school, restaurant, household, pet, animal husbandry, culturing farm and the like.

The invention belongs to the technical field of zoonosis prevention, and particularly relates to a mycoplasma bovis protein gene MbovGdpP and application thereof. Mbov_0276 is artificially synthesizedaccording to the mycoplasma bovis HB0801 genome sequence. An Mbov_0276 gene is modified for the preference of escherichia coli for codons; mycoplasma bovis tryptophan codons UGA are mutated into codons UGG for encoding tryptophan in escherichia coli to obtain escherichia coli recombinant proteins rMbovGdpP. The Mbov_0276 gene has the nucleotide sequence shown in SEQ ID NO:1, and the ended proteinhas the sequence shown in SEQ ID NO:2. A mutant strain T6.290 has the growth defect phenotype, has the small colony morphology on a PPLO culture medium, reduces the adhesion of EBL cells and improvesthe sensitivity to saline ions. The mutant strain can be expected to be applied in the mycoplasma bovis pathogenesis and the preparation of immunosuppressive medicines.

The invention relates to the field of zoonosis immune diagnosis and discloses a brucellosis antibody detection immunochromatography test strip based on colloidal gold as a marking material and a preparation method of the test strip. In a quick brucellosis antibody detection technology, the 40-nm colloidal gold labeled with staphylococcus aureus protein A (SPA) is sprayed on glass fibers to form a gold labeled pad. Genes OMP31 and BP26 are cloned from a brucella genome, form prokaryotic expression recombinant plasmids and are transformed in escherichia coli to express proteins omp31 and bp26, the two proteins as coating antigens are respectively coated on a nitrocellulose membrane to serve as detection lines, and the detection lines, the gold labeled pad, a specially treated sample loading pad and water absorption paper are assembled into an immunochromatography detection device. The test strip has the characteristics of strong specificity, high sensitivity, convenience, simplicity, economy and the like, can be applied to typing detection of brucellosis antibodies of sheep and cattle, and has very important meaning and practical application value for brucellosis monitoring and prevalence control.

The invention belongs to the technical field of zoonosis prevention and treatment, and particularly relates to a mycoplasma bovis mutant strain with a growth defect under cell coculture and application. The mycoplasma bovis Mbov-0328 gene deletion mutant strain T9.386 is sifted out from a mycoplasma bovis gene mutant library, and the gene is coded to form cyclic dinucleotide phosphodiesterase. When the mutant strain and a bovine embryo pneumonocyte are co-cultured, the remarkable growth defect phenotype is shown; the mutant strain shows small colonial morphology on a PPLO solid culture medium.Proteomics between the mutant strain and a wild strain shows a remarkable difference expression spectrum. The mutant strain has 38 differential expression proteins, wherein 30 proteins are in up-regulated expression, and 8 proteins are in down-regulated expression. The mutant strain can be applied to the field of mycoplasma bovis metabolic physiology, pathopoiesia and immune prevention.

The invention discloses a various important aquagenic zoonoses protozoa simultaneous assay kit, and further provides a preparation method of the kit. A primer and a probe are designed according to the special gene sequence of the giardia lamblia, the cryptosporidium parvum and the toxoplasma gondii by a multi-fluorescence quantitative PCR (polymerase chain reaction) specific assay method, the sensitivity is improved by optimizing the fluorescence quantitative PCR reaction system and condition, the amplification can be performed, and an amplification result can be directly and timely observed.The invention can be used for fast and exactly assaying three important aquagenic zoonoses protozoa, and has the characteristics of being precise in design, simple and easy to operate, high in sensitivity, high in specificity, exact and objective in judgment, and the like.

The invention relates to a method for constructing tissue-engineering skin. The construction method adopts an autocrine extracellular matrix of fibroblast as a biological scaffold of the tissue-engineering skin. The method utilizes the extracellular matrix excreted by the cultured fibroblast as the biological scaffold and human epidermal cells as seed cells, and the cultured tissue-engineering skin is closer to the structure of natural skin. The method has the advantages that: firstly, the method is simple and convenient, and favorable for synthesis and mass production of products because thematerials take the form of liquid; secondly, the obtained product has the structure which is closer to the natural skin, and the method is favorable for improving the sensibility and the precision ofprediction of the toxicity of compounds; thirdly, the product does not contain additional biomaterials, so that the risk of cross infection of zoonosis cannot occur; and fourthly, the obtained product is convenient for quality control, so that the variation between batches is reduced.

The invention discloses a fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Leishmania donovani, relating to gene detection technology of zoonosis pathogen, and being applicable to qualitative and quantitative detection of Leishmania donovani. The invention comprises a standard positive template, a fluorescent quantitative PCR reaction solution, Leishmania donovani kDNA gene specific primer and negative quality control standard product. The invention is accurate in quantifying, rapid in detection speed, good in specificity, high in sensitivity, simple in use steps and high in repeatability and can replace traditional etiology detection method.

The invention discloses a fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Toxoplasma gondii, relating to gene detection technology of zoonosis pathogen, and being applicable to qualitative and quantitative detection of Toxoplasma gondii. The invention comprises DNA extracting solution, a standard positive template, a fluorescent quantitative PCR reaction solution, Toxoplasma gondii B1 gene specific primer and a negative quality control standard product. The invention is accurate in quantifying, rapid in detection speed, good in specificity, high in sensitivity, simple in use steps and high in repeatability and can replace traditional etiology detection method.

The invention discloses an mRT-PCR (multiple reverse transcription-polymerase chain reaction) kit for discriminating and detecting important avian economic animal viruses and an application of the mRT-PCR kit. The kit comprises primer pairs for detecting an AIV (avian influenza virus) M gene, AIV H7,H9 and H5 subtypes, an NDV (newcastle disease virus), an ILTV (infectious laryngotracheitis virus), an IBDV (infectious bursal disease virus) and an EDS (egg drop syndrome) virus. The mRT-PCR kit has the characteristics of high sensitivity, high specificity and the like when used for detecting AIVs and other common respiratory viruses, can be used for immediately detecting and discriminating important and popular subtypes of the AIVs and five avian respiratory pathogens most popular in China and has a great significance in zoonosis control, monitoring, prevention and control of avian mixed infection, biosafety of farms, reduction of loss of the breeding industry, food safety, quarantine and the like.

The invention discloses a method for diagnosing five varieties of disease virus infected by both human beings and animals and a special kit thereof. The kit comprises a primer set A used for diagnosing foot and mouth disease virus: the DNA indicated by the sequence 1 in the sequence table, and the DNA indicated by the sequence 2 in the sequence table. The method of using the kit for diagnosing viruses has the advantages of high specificity and reliable results. When using a plurality of varieties of primers for diagnosing simultaneously, the diagnosis of the plurality of varieties of viruses in an identical PCR reaction can be achieved, the diagnosis efficiency is high, time and reagent are saved greatly and cost is reduced; therefore, the method has convenient, rapid and sensitive advantages, has significance for enhancing the monitoring of disease virus infected by both human beings and animals and for preventing the propagation of the disease virus infected by both human beings and animals.

The invention discloses a polymerized peptide and a gel having a collagen-like structure. Although collagen is useful as a medicinal material, its use in humans involves the risks of allergy and zoonoses. Therefore, artificial collagens and the like are being studied as medicinal materials. However, typical artificial collagens cannot be considered completely sufficient, given their lack of physical strength, being denatured by heat, inability to be processed into a sheet, and the like, and thus being problematic in terms of practicality. In the present invention, a collagen-like peptide having a triple helical structure comprising triple strands of peptide chains including a plurality of cysteine (cys) residues is oxidized and cross-linked to prepare a polymerized peptide. This polymerized peptide may be processed into and used as a hydrogel and a thin-film sheet. In addition, by inserting a functional amino acid sequence present in biomacromolecules, particular physiological functions such as cell adhesiveness may be obtained. Further, the present invention provides: methods for preparing the polymerized peptide and the like; and uses thereof as compositions in cell cultures, wound coverings, regenerative medicinal materials, and research materials and the like by using the same.

The invention discloses a decellularized black carp skin biomimetic substrate for regeneration restoration as well as a preparation method and application thereof. The decellularized black carp skin biomimetic substrate for regeneration restoration is obtained by using black carp skin as raw materials through special treatment. The preparation method of the decellularized black carp skin biomimetic substrate for regeneration restoration comprises the process steps of raw material pretreatment, coarse finishing, degreasing, decellularizing, re-finishing, storage liquid pretreatment, shaping andthe like. The decellularized black carp skin biomimetic substrate for regeneration restoration prepared by the invention has the advantages that the substrate cell removal is thorough; the natural porous network structure is complete; the material is the biomimetic cell outer substrate support frame material with low immunogenicity; good micro environment can be provided for the tissue regeneration restoration and cell growth. Compared with common use decellularized substrate products from pigs, cows and the like, the decellularized black carp skin biomimetic substrate prepared by the invention has the advantages that the zoonosis virus carrying and spreading risk is low; the religious ethic risk is low; the resources are rich; the cost is low.

The invention discloses a TaqMan probe for real-time fluorescence PCR (Polymerase Chain Reaction) detection of angiostrongylus cantonensis infected snails on the basis of a modern molecular biological technology, which is a detection method applied to snails infected with angiostrongylus cantonensis. The angiostrongylus cantonensis is food-borne zoonosis and is infected mainly through eating infected snails by mistake. The invention aims to provide a rapid and accurate method for detecting the snails infected with the angiostrongylus cantonensis. The TaqMan probe is technically characterized in that (1) a primer(upstream) having the sequence of 5'-TGA TGC TTG TGC GGT TTT C-3', a primer- (downstream) having the sequence of 5'-CCGAAG GCA AAG CAC ACA C-3' and a probe having the sequence of5'-CGC TAT ACA CGC ACA TTT GCC GG-3' are provided; (2) the total volume of a reaction system is 20 mu L, wherein the reaction system comprises 10 muL of 2*Taq PCR MasterMix, 1 muL of Taqman probe, 1 muL of the upstream primer, 1 muL of the downstream primer, 1 mu L of template and the balance of ddH2O; and (3) in a reaction process, the pre-degeneration time is 3min at 94 DEG C, 10s at 94 DEG C and 30s at 60 DEG C, and the circulation is carried out for 40 times. The invention provides a new technological measure for food quarantine as well as investigation and monitoring of epidemic situations and has far reaching importance on preventing and controlling the occurrence of the angiostrongylus cantonensis diseases.

The invention provides a compound bactericide with bacteriostatic or bactericidal activity. The composition comprises, by weight, 40% to 50% of linalool, 18% to 25% of terpilenol, 8% to 15% of geraniol, 2% to 4% of beta-cyclocitral, 2.5% to 5% of nerol, 1% to 3% of myrcene, 1% to 3% of ocimene, 3% to 6% of ionone and 5% to 8% of 2H-beta-ionone. Antibacterial and bacteriostatic drug sensitivity tests show that the bactericide artificially prepared on the basis of natural essential oil components has a broad-spectrum inhibition effect on bacteria and fungi. The bactericide has bacteriostatic andbactericidal effects on bacteria (escherichia coli, bacillus subtilis, staphylococcus aureus, staphylococcus epidermidis and the like) and fungi (candida albicans, Schlechospora crassa, trichophytonrubrum, saccharomyces cerevisiae and the like), and particularly has an obvious effect on zoonosis. The bactericide can be applied to development of zoonosis infection and other sterilization products.

The invention relates to the field of zoonosis immunologic diagnosis and discloses a brucellosis antibody test strip and a preparing method thereof. The brucellosis antibody test strip is prepared in a way that colloidal gold with a grain diameter of 30nm is first prepared, used as an indication mark for marking and reconstructing large peptostreptococcus proteins L, dissolved in a gold mark work solution and sprayed on glass fibers to make a gold mark pad; VirB5 genes are cloned from a brucella genome and connected to pET-28a to form an expression vector; the expression vector is converted into escherichia coli, VirB5 proteins are expressed and coat on a nitrocellulose membrane after separation and purification as a detection line, IgGs (Immunoglobulin G) bonded with the proteins L are coated on the nitrocellulose membrane as a quality control line, and an immunochromatography detection device is made. The brucella immunochromatography device has the characteristics of strong specificity, high sensitivity, great stability, simplicity, economy, rapidness and the like and has extremely significant actual application value in the monitoring and epidemic control of zoonosis.

The invention discloses a gene chip for viruses of zoonosis-borne diseases and application thereof. The gene chip for the viruses of the zoonosis-borne diseases is a gene chip for detecting at least one virus in six viruses of hepatitis E virus, Nipah virus, rabies virus, Rift Valley fever virus, foot and mouth disease virus and Vesicular stomatitis virus, and is fixed with a hepatitis E virus detection probe, a Nipah virus detection probe, a rabies virus detection probe, a Rift Valley fever virus detection probe, a foot and mouth disease virus detection probe and a Vesicular stomatitis virus detection probe. The invention also discloses a method for detecting the Rift Valley fever virus, the Nipah virus, the foot and mouth disease virus, the Vesicular stomatitis virus, the hepatitis E virus and / or the rabies virus. The invention can stably and specifically implement generic detection of the six viruses of the zoonosis-borne diseases, has the advantages of simpleness, convenience, quickness, good specificity and high sensitivity, and can be used for detecting clinical samples.

A traditional Chinese medicine for control of animal Escherichia coli disease and a preparation method thereof belong to the field of traditional Chinese medicine for animals. The aim of the invention is to provide a modern novel traditional Chinese medicinal veterinary preparation for prevention and treatment of coli diseases of pig, chicken, cattle, sheep, horse, dog and rabbit and a preparation method thereof. The formula of the invention comprises Herba euphorbiae humifusae, Rhizoma Coptidis and Radix pulsatillae. Guided by traditional Chinese veterinary medicine theory, the medicine provided by the invention employs the formula aiming at Escherichia coli diseases of poultry and livestock, reduces or replaces antibiotics and other chemical medicines, solves the problems of more and more serious resistance of Escherichia coli and excessive drug residues in meat, egg, milk and other animal products. The medicine is more safe, effective and controllable in quality, can be used in prevention and control of Escherichia coli diseases of animals, and has great practical significance in the promotion of the development of animal industry, prevention and control of zoonoses and maintenance of food safety.

The invention discloses a drug for preventing and treating animal respiratory syndrome and belongs to the field of animal drugs. The drug is guided by theory of traditional Chinese veterinarian and used for preventing and treating the animal respiratory syndrome. The drug is composed of Cynanchum glaucescens, Radix stemonae, Houttuynia cordata, Fructus chebulae, Herba andrographitis and licorice root. The drug can effectively prevent and treat the animal respiratory syndrome. On the basis of exploring advantages and characteristics of traditional Chinese veterinarian, a preparation which combines traditional Chinese medicine with Western medicine and is safer, effective and controllable in quality is studied and developed to prevent and treat the animal respiratory syndrome by adhering to rules combining traditional Chinese medicine with Western medicine, and the drug has great economic and social benefit in promoting development of animal farming, preventing and controlling zoonosis and maintaining food safety.

The invention discloses a preparation method of degradable antibacterial hemostatic hydrogel capable of promoting diabetic wound repair. The hydrogel with a three-dimensional network structure is prepared from dopamine (DA) grafted oxidized natural polysaccharide and jellyfish collagen (JC) through a cross-linking agent. The hydrogel prepared by the invention not only has excellent tissue adhesion performance, antibacterial performance and hemostasis performance, but also has very strong water retention capacity, provides a moist environment for wound healing of diabetes mellitus, and accelerates wound healing. The invention has the advantages that: 1, the prepared degradable hydrogel with antibacterial and hemostatic functions for repairing the diabetic wound surface does not need to be additionally added with antibiotics when being used for treating the diabetic wound surface, so that the generation of drug resistance is avoided; 2, the used collagen is derived from plankton in an aquatic environment, and compared with animal-derived collagen, outbreak of zoonosis (mad cattle disease, porcine brucellosis, inhalable anthrax and the like) and some potential virus transmission risks are avoided;

A schistosomiasis japonica arborization carrier-DNA vaccine and a preparation method thereof relate to a zoonosis nucleic acid vaccine, in particular to a nucleic acid vaccine for schistosomiasis, and belong to the biotechnology field. The invention is formed by combining schistosomiasis DNA vaccine pJW4303-SjC23 and modified arborization carrier PAMAM-Lys into a compound of schistosomiasis japonica arborization carrier-DNA vaccine PAMAM-Lys / pJW4303-SjC23. The preparation method comprises the following steps: (1) constructing and preparing DNA vaccine pJW4303-SjC23, (2) preparing polyamide-amine arborization macromolecule, wherein quadrol performs modification on the terminal group of the core PAMAM, and (3) preparing the arborization carrier-DNA vaccine. The arborization carrier-DNA vaccine constructed by combining schistosomiasis japonica DNA vaccine pJW4303-SjC23 and PAMAM-Lys has the functions of obviously improving host immunoreactivity and immunoprotection, and has excellent biocompatibility with the host histiocyte. The vaccine has a very wide application prospect.

The invention belongs to the research field of zoonosis, relating to a differential diagnosis method. Nonstructural protein NS1 gene in a disclosed influenza A H1N1 California virus strain (A / California / 08 / 2009(H1N1)) cDNA sequence is taken as the research content, a gene synthesis method is used to obtain a gene fragment construction prokaryotic expression carrier Pgex-6P-1-NS1; positive recombinant plasmid is converted to colibacillus to obtain a recombination strain (Escherichia coli BL21 rosseta / Pgex-6p-1-NS1); the nonstructural protein NS1 of the purified H1N1 is obtained by a plurality of chromatography methods, and an enzyme linked immunosorbent assay differential diagnosis method is established in a manner that the expression protein obtains specificity through a immunization host animal specific to polyclonal or monoclonal antibody of the H1N1 nonstructural protein NS1, which is used for distinguishing patients suffering from influenza A H1N1 virus infection and other patients suffering from influenza virus infections.

The invention belongs to the field of biology, and particularly discloses bacillus velezensis D2406 separated from a stratiomyiid intestinal tract and application thereof. The preservation number of the bacillus velezensis is CCTCC NO: M2020874, and the bacillus velezensis can promote growth of hermetia illucens and can stably inhibit staphylococcus aureus under the in-vitro condition and in the body of the stratiomyiid larva. The intestinal bacteria disclosed by the invention can improve the conversion rate of larvae, the reduction rate of materials and the weight gain rate of the larvae, and can stably inhibit staphylococcus aureus under the in-vitro condition and in the body of stratiomyiid larva. The volatile compound 2,5-dimethyl pyrazine produced by the strain has a remarkable inhibition effect on zoonosis pathogenic bacteria. The intestinal bacteria are inoculated into a hermetia illucens transformation system, so that the hermetia illucens transformation efficiency is improved, the abundance of staphylococcus aureus in a transformation material is reduced, and environmental pollution caused in the use process of chemical drugs is expected to be reduced.

The invention discloses a prevention system of zoonosis and a prevention method thereof. The method comprises the steps of storing a pet vaccinating record on a near field chip of a pet end and storing a user vaccinating record on a portable device with a Near Field Communication function, receiving the pet vaccinating record and transmitting the pet vaccinating record together with the user vaccinating record to a server side through the network when the portable device with the Near Field Communication function senses the near field chip of the pet end, making the server side conduct comparative analysis and generate a suggestion report according to the vaccinating records, and then transmitting the suggestion report to the portable device to be outputted. In this way, the technical effect of improving the convenience in preventing zoonosis is achieved.

The invention discloses a TaqMan probe for real-time fluorescence PCR (Polymerase Chain Reaction) detection of angiostrongylus cantonensis infected snails on the basis of a modern molecular biological technology, which is a detection method applied to snails infected with angiostrongylus cantonensis. The angiostrongylus cantonensis is food-borne zoonosis and is infected mainly through eating infected snails by mistake. The invention aims to provide a rapid and accurate method for detecting the snails infected with the angiostrongylus cantonensis. The TaqMan probe is technically characterized in that (1) a primer(upstream) having the sequence of 5'-TGA TGC TTG TGC GGT TTT C-3', a primer- (downstream) having the sequence of 5'-CCGAAG GCA AAG CAC ACA C-3' and a probe having the sequence of 5'-CGC TAT ACA CGC ACA TTT GCC GG-3' are provided; (2) the total volume of a reaction system is 20 mu L, wherein the reaction system comprises 10 muL of 2*Taq PCR MasterMix, 1 muL of Taqman probe, 1 muL of the upstream primer, 1 muL of the downstream primer, 1 mu L of template and the balance of ddH2O; and (3) in a reaction process, the pre-degeneration time is 3min at 94 DEG C, 10s at 94 DEG C and 30s at 60 DEG C, and the circulation is carried out for 40 times. The invention provides a new technological measure for food quarantine as well as investigation and monitoring of epidemic situations and has far reaching importance on preventing and controlling the occurrence of the angiostrongylus cantonensis diseases.

The invention discloses an animal waste sampling device which comprises a spoon head (3) and a handle (4), the front end (2) of the spoon head protrudes and is blunt-round, and the upper part (1) of the front end is hooked backward; the rear end tapers gradually and is connected with the handle, the spoon head (3) is in a spherical body which is larger than a semiellipse, and the opening edge of the spoon is blunt. According to the animal waste sampling device, a hand is not required to enter the rectum directly, the labour intensity is reduced, stress to the animal can be reduced, and the damage probability to the animal rectum mucosa can be reduced. Pollution to the arm and cloth of an operator can be avoided during waste sampling, and zoonosis spreading can be reduced. The operation is easy and convenient, and the animal waste sampling device can be used aiming at different large and medium-sized animals. The collected waste sample size is adequate, and test detection requirements are ensured. The animal waste sampling device can be reused after cleaning and autoclaved sterilization so as to save the cost.

The invention discloses a gene-deleted recombinant bacillus calmette-guerin vaccine, and belongs to the technical field of zoonosis prevention and treatment. The gene deleted by the strain is a BCG_0349 gene with a nucleotide sequence shown as SEQ ID NO: 1. A homologous exchange substrate containing upstream and downstream homologous arms of the BCG_0349 gene is transduced into a bacillus calmette-guerin vaccine genome in a thermo-sensitive phage transducing mediated homologous recombination mode to knock out the BCG_0349 gene, the strain is successfully constructed, and it is confirmed for the first time that a deleted strain can promote expression and secretion of inflammatory cytokines of the organism and is weakened in toxicity; and specifically, the reduction of the forming ability ofthe soxhlet structure and the reduction of the phenotype and intracellular survival ability of small bacterial colonies are easier for removal of organisms, and the vaccine has good application potential in the related fields of tuberculosis diagnosis of human and animals, vaccine and drug development and the like.

The invention relates to a biological plant stem cell disinfection and purification liquid integrated device for preventing zoonosis diseases, belongs to the technical field of disease prevention, and aims to solve the problem that alcohol cannot be disinfected for multiple times due to the fact that the alcohol is easy to volatilize during use, and comprises a disinfection and purification box and a placement bottom plate fixedly mounted on the bottom surface of the disinfection and purification box, a partition plate is fixedly installed on the inner wall of the middle of an inner cavity of the disinfection and purification box, a plugging block is installed in an inner cavity of the middle of the partition plate in a penetrating mode, a plugging cover is installed on the top face of the disinfection and purification box, a plugging downward-pushing column is fixedly installed in the middle of the bottom face of the plugging cover, and a discharging box is arranged at the lower end of the plugging downward-pushing column in a sealed and sliding mode. According to the disinfection and purification device for the stem cell tissue of the biological plant, an operator can conduct one downward pushing operation, disinfection and purification of the stem cell tissue of the biological plant are completed, meanwhile, it is guaranteed that alcohol does not make contact with the external environment all the time in the disinfection and purification process, and volatilization of the alcohol is reduced.

The invention provides a multiple DPO-PCR (dual priming oligonucleotide-polymerase chain reaction) primer combination for detecting trichinella spiralis, toxoplasma gondii and schistosome. The sequences of the primer combination are as shown in SEQ ID NO:1 to SEQ ID NO:6 correspondingly. The invention also provides a multiple DPO-PCR method for detecting the trichinella spiralis, the toxoplasma gondii and the schistosome. By adopting the method, qualitative detection on the muscular tissue or viscera sample of animals suffering from diseases can be realized. Experiments indicate that the detection method provided by the invention has high specificity, high flux and high speed, the detection efficiency is improved, and a more effective method is provided for detecting three animal origin parasitic zoonoses of the trichinella spiralis, the toxoplasma gondii and the schistosome.

The invention discloses a prevention system of zoonosis and a prevention method thereof. The method comprises the steps of storing a pet vaccinating record on a near field chip of a pet end and storing a user vaccinating record on a portable device with a Near Field Communication function, receiving the pet vaccinating record and transmitting the pet vaccinating record together with the user vaccinating record to a server side through the network when the portable device with the Near Field Communication function senses the near field chip of the pet end, making the server side conduct comparative analysis and generate a suggestion report according to the vaccinating records, and then transmitting the suggestion report to the portable device to be outputted. In this way, the technical effect of improving the convenience in preventing zoonosis is achieved.