103 results about "Respiratory pathogens" patented technology

The invention provides a micro-fluidic chip reagent kit for detecting ten respiratory tract infection pathogens and a use method of the reagent kit. The reagent kit adopts a combination of a Taqman probe fluorescent PCR (polymerase chain reaction) technology and a micro-fluidic chip, detects the ten common respiratory tract infection pathogens, can obtain a detection result within 2h, and is highin specificity, and the sensitivity can reach 100 copies / microliter. The kit comprises a sample introduction chamber, at least ten reaction chambers and a micro-fluidic flow channel, wherein the reaction chambers are mutually independent; each reaction chamber is provided with a reagent dry powder for amplifying one respiratory tract pathogen in advance; each reagent dry powder comprises a primerfor amplifying the corresponding respiratory tract pathogen and a TaqMan probe; the reagent dry powder arranged in each reaction chamber in advance can amplify any one of the ten respiratory tract pathogens; the respiratory tract pathogens amplified by the reagent dry powder in all the reaction chambers can include the ten respiratory tract pathogens.

The invention discloses a respiratory pathogen multi-detection reagent kit. The respiratory pathogen multi-detection reagent kit has the advantages that the respiratory pathogen multi-detection reagent kit is based on multi-PCR (polymerase chain reaction) technologies, detection results can be determined by the aid of fluorescence resonance energy transfer via the melting temperature ranges, the respiratory pathogen multi-detection reagent kit can be used for qualitatively simultaneously detecting 16 types of respiratory pathogens, the 16 types of respiratory pathogens include 12 types of RNA(ribonucleic acid) viruses (influenza A viruses, influenza B viruses, H1N1 influenza A viruses, type A and type B respiratory syncytial viruses, type -1 / -2 / -3 parainfluenza viruses, type OC43 coronaviruses, type 229E coronaviruses, rhinoviruses and human metapneumovirus), 2 types of DNA (deoxyribonucleic acid) viruses (adenoviruses and bocavirus) and 2 types of bacteria (mycoplasma pneumoniae andbordetella pertussis), the respiratory pathogen multi-detection reagent kit is high in detection sensitivity, and the sensitivity even can reach 1 copy / reaction; the multi-detection reagent kit is good in specificity, and negative results of pathogens which have identical sampling sites and similar pathogenic mechanisms and are not in the detection range of the respiratory pathogen multi-detectionreagent kit can be obtained; the respiratory pathogen multi-detection reagent kit is short in operation time and easy to operate and can be used for quickly detecting the 16 types of respiratory pathogens in a single tube of a reaction system, the results are clear and are easy to interpret, and the like.

The invention provides a kit for detecting 10 respiratory tract infection pathogens and an application method thereof. The kit comprises primers and a TaqMan probe, wherein the primers are used for amplifying the 10 respiratory tract infection pathogens which include mycoplasma pneumoniae, chlamydia pneumoniae, legionella pneumophila, bordetella pertussis, rhinovirus, respiratory tract adenovirus, influenza A virus, influenza B virus, respiratory syncytial virus and parainfluenza virus. The application method includes: mixing 2 microliters of sample DNA / RNA co-extraction template, 20 microliters of RT-PCR buffer, 1 microliter of mixed enzyme liquid and 2 microliter of primer-probe mixed liquid to perform PCR amplification reaction. The kit uses the Taqman probe fluorescent PCR technology to detect the 10 common respiratory tract infection pathogens, a detection result can be obtained within 2 hours, the kit is high in specificity, and the sensitivity of the kit can reach 100copies / microliter.

The invention relates to a primer composite for detecting respiratory pathogens. The primer composite comprises at least one group of a mycoplasma pneumoniae group, a chlamydia pneumoniae primer group, an influenza A / B virus primer group, a parainfluenza virus primer group, an adenovirus primer group and a respiratory syncytial virus primer group. The invention further relates to a kit comprising the primer composite. The kit further comprises a micro-fluidic chip wrapping primers, and a macroscopic indicator. The invention further relates to a detection method adopting the primer composite. The detection method comprises the steps of primer composite coating, to-be-detected sample nucleic acid extraction, LAMP reaction and visual result interpretation. The kit and the method are applied to the micro-fluidic chip for visual judgment, instant detection of the seven respiratory pathogens is rapidly and accurately realized, and a result is judged by a naked eye by color change, so that the kit and the method are simpler, more convenient and quicker in practical applications, are easy to operate, and are suitable for site operation.

The invention provides a primer and probe combination for multiple respiratory tract pathogen nucleic acid detection and typing identification of new coronaviruses based on multiple PCR time-of-flightmass spectrometry. The primer and probe combination comprises: 1) a primer sequence combination as shown in Table 1; and 2) a probe sequence combination as shown in a table 2. The invention also provides a kit for multiple respiratory tract pathogen nucleic acid detection and typing identification of new coronaviruses based on multiple PCR time-of-flight mass spectrometry. According to the invention, based on a MassARRAY MALDI-TOF MS system, multiple respiratory tract pathogens can be detected at the same time, and typing identification can also be carried out on the 2019 novel coronavirus (2019nCoV), so that the kit has more significance in clinical and virus research.

The invention relates to the field of medical examination, in particular to a multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens. The multi-connected detection reagent card comprises multiple sample pads and multiple nitrocellulose membranes connected with absorbent paper, wherein each sample pad is covered with multiple fluorescent microspheres, and the surface of each fluorescent microsphere is covered with an antibody of one respiratory pathogen; each nitrocellulose membrane connected with the absorbent paper is provided with at least two detection lines, and the detection lines are arranged at the nitrocellulose membrane; each detection line is fixedly covered with an antibody of one respiratory pathogen, and the antibody is in a pairing relation with the antibody, covering the surface of one fluorescent microsphere covering the corresponding sample pad, of one respiratory pathogen. The reagent card is simple, convenient and rapid to operate; different specific test strips can be formed through matching of different nitrocellulose membranes connected with the absorbent paper and the sample pads, different test strips are matched in a mixed manner and put in card paper according to demands, the detection flexibility and the customization property are high, and the detection cost is saved.

The invention discloses a method for detecting 29 respiratory pathogens by using a Taqman low-density microfluidic chip (TAC) technology. According to the method, the TAC detection technology is established for the 29 respiratory pathogens, the advantages of MGB probe detecting are fully used, the defects of previous multiple detection of the respiratory pathogens are overcome, 29-respiratory-pathogen multi-target detection of the respiratory pathogens can be achieved on a sample in two hours, and the good sensitivity and the good specificity are achieved. According to the method for detectingthe 29 respiratory pathogens by using the TAC technology, a quick, accurate and high-throughput technological mean is provided for monitoring and outbreak investigation of respiratory diseases in China.

The invention provides dual-target site inverse-transcription fluorescent PCR primers, probes and kits for detection of 2019 new coronavirus (2019-nCoV). Nucleotide sequence alignment is performed onthe whole genome of the 2019-nCoV, 3 specific nucleotide sequences W1, W2 and W3 are found in N genes of the virus, and are shown in SEQ ID No. 1-3, multiple pairs of primer probes are designed separately for three target sites, and it is found that when a primer probe combination which is designed for the target sites of W1 and W2 and has sequences shown in SEQ ID No. 4-6 and SEQ ID No. 7-9 is applied to double-target site inverse-transcription fluorescent PCR, 2019-nCoV can be detected specifically and sensitively with detection sensitivity of within 10 copies; and no non-specific amplification of 22 clinically-common respiratory pathogens are caused, false-negative results caused by single target spot mutation can be avoided effectively, and good detection ability of specimens is achieved.

Concentrated vapors from botanical essential oils are inhaled to prevent, treat and cure infections of the respiratory pathogens causing Severe Acute Respiratory Syndrome (“SARS”). These vapors are inhaled as a practical method to reduce the risks of infection by the pathogens causing SARS in crowded public places. These vapors are also inhaled as a practical method to reduce the risks of infection by unknown, and unpredictable, respiratory pathogens that may be present in public places. The essential oils have antiseptic properties, are safe to inhale, and include, but are not limited to, the essential oils from Eucalyptus globulus, Melaleuca alternifolia, Eucalyptus citriodora, and Eucalyptus radiata. Convenient hand-held inhaler apparatus are provided for the inhalation of concentrated vapors from the antiseptic essential oils and other substances. The antiseptic essential oils have selected antiviral, antibacterial, and antifungal properties.

The invention discloses a probe and primer composition for rapidly detecting seven coronaviruses and other respiratory tract pathogens. A group of specific primers and the Taqman or MGB probe are designed mainly according to the specificity of N genes of OC43-CoV, NL63-CoV, 229E-CoV, HKU1-CoV, SARS-CoV, FluB and RSV, ORF1a, ORF1b and N genes of MERS-CoV, ORF1ab and N genes of 2019-nCoV, an M geneof FluA and an HEXON gene of HadV, and by utilizing a melting curve technology, CoV-OC43, HCoV-NL63, HCoV-229E, HCoV-HKU1, SARS-CoV, MERS-CoV, 2019-nCoV, FluA, FluB, HAdV and RSV are rapidly identified according to the change of the melting temperature. By means of the design, multiple pathogens can be detected in one fluorescent channel at the same time, and a relatively high sensitivity can be met.

The invention discloses a kit for simultaneously detecting nucleic acids of seven respiratory pathogens and application of the kit. The kit is based on a double amplification technology (RNA isothermal amplification and multi-biotin signal amplification), and can detect H1N1 / H3N2 type influenza A viruses, influenza B viruses, respiratory syncytial viruses, 1 / 2 / 3 type human parainfluenza viruses, B / E type adenoviruses, mycoplasma pneumoniae and chlamydia pneumoniae. The kit of the invention does not need RNA extraction, and is not prone to pollution, high in sensitivity and good in specificityin the process of detection, and can be widely applied to detection of the nucleic acids of the above seven respiratory pathogens.

The invention provides a primer group capable of simultaneously detecting 27 respiratory tract pathogens based on a nucleic acid mass spectrometry technology, a kit and application, and belongs to thefield of pathogen nucleic acid detection. According to the kit, PCR amplification primers and single-base extension primers are designed for 27 clinically common respiratory tract pathogens. Pathogenic bacteria corresponding primer and single base extension primer sequences are shown in SEQ ID NO: 1-81. According to the nucleic acid mass spectrometry, a multiple RT-PCR technology and a single base extension technology are integrated, pathogen target genes are amplified through multiple RT-PCR, and the detection sensitivity is improved; in the single-base extension reaction, one base amplification is carried out on an extension primer, and pathogen identification is carried out by distinguishing the molecular mass of an extension product, so that the specificity is high. Meanwhile, the flux of the nucleic acid mass spectrum is high, amplification of 40 genes can be achieved at most through one reaction hole, and 384 samples can be analyzed at most at a time by means of the chip technology; the kit has the advantages of high automation degree, high detection flux, high sensitivity and good specificity.

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

The invention discloses a PCR (polymerase chain reaction) primer group, a probe set and a kit for detecting multiple respiratory pathogens. Sequences of PCR primers are represented as SEQ ID NO.1-SEQ ID NO.12; probes comprise at least two of six molecular beacon probes for respiratory pathogens, a fluorescence group and a quenching group are formed at two ends of each molecular beacon probe respectively, and sequences are represented as SEQ ID NO.13-SEQ ID NO.18. The invention further discloses a PCR reaction liquid containing the PCR primers and the probes as well as a kit containing the PCR reaction liquid for at least one person. With the adoption of the PCR primer group, the probe set and the kit, the six respiratory pathogens can be rapidly and accurately detected in a typing manner.

The invention provides a kit for multiple detection of respiratory pathogens. Specifically, according to the kit, a PCR amplification system of multiple respiratory pathogens is designed and verifiedby the experiment, a multiple fluorescent PCR amplification system for detecting the multiple respiratory pathogens is obtained, and three respiratory pathogens can be detected simultaneously by a single system. The kit has high sensitivity and specificity, and according to the kit, the multiple respiratory pathogens in samples of nasopharynx swab, alveolar lavage fluid, sputum and the like can bedetected and analyzed quickly.

The invention provides a primer, product and method for detecting feline respiratory tract pathogens as well as application, and relates to the technical field of nucleic acid tests. In the primer group for detecting the feline respiratory tract pathogens, a primer for detecting feline herpesvirus type 1 (FHV1), a primer for detecting feline calicivirus (FCV), a primer for detecting mycoplasma, aprimer for detecting feline chlamydia and a primer for detecting bordetella bronchiseptica are primers designed through highly conservative regions of each pathogen, have no cross reaction with otherfeline pathogens, and have the characteristics of high specificity and high sensitivity. By use of the primer group, five feline respiratory tract pathogens including the FHV1, the FCV, the mycoplasma, the feline chlamydia and the bordetella bronchiseptica can be identified in the same reaction system, and the detection cost and time are greatly reduced as compared with a traditional method.

The invention discloses an mRT-PCR (multiple reverse transcription-polymerase chain reaction) kit for discriminating and detecting important avian economic animal viruses and an application of the mRT-PCR kit. The kit comprises primer pairs for detecting an AIV (avian influenza virus) M gene, AIV H7,H9 and H5 subtypes, an NDV (newcastle disease virus), an ILTV (infectious laryngotracheitis virus), an IBDV (infectious bursal disease virus) and an EDS (egg drop syndrome) virus. The mRT-PCR kit has the characteristics of high sensitivity, high specificity and the like when used for detecting AIVs and other common respiratory viruses, can be used for immediately detecting and discriminating important and popular subtypes of the AIVs and five avian respiratory pathogens most popular in China and has a great significance in zoonosis control, monitoring, prevention and control of avian mixed infection, biosafety of farms, reduction of loss of the breeding industry, food safety, quarantine and the like.

The invention discloses a collaborative quadruple real-time fluorescence PCR detection method for simultaneously detecting target nucleic acids of nine pathogens by using three PCR reaction tubes. Themethod is used for detecting influenza A virus (FluA), influenza B virus (FluB), parainfluenza virus (PIV), respiratory syncytial virus (RSV), mycoplasma pneumoniae (MP), chlamydia pneumoniae (Cpn),adenovirus (ADV), human rhinovirus (HRV) and human metapneumovirus (hMPV) in samples. The method is rapid to operate, can be completed by one-step cooperation and has high sensitivity. In addition, the invention also relates to a reagent involved in the PCR method, a detection kit used for the method, preparation and application of the detection kit and the like.

The invention discloses a multi-fluorescent immunoassay method for rapidly distinguishing 6 types of poultry respiratory pathogens. The multi-fluorescent immunoassay method is simple to operate; a target amplified fragment is obtained through a PCR (Polymerase Chain Reaction); then an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector to distinguish viruses of different types. According to the method disclosed by the invention, avian influenza viruses, chicken infectious bronchitis viruses, chicken Newcastle disease viruses, chicken infectious laryngotracheitis viruses, mycoplasma gallisepticum and mycoplasma synoviae can be accurately detected at the same time; the multi-fluorescent immunoassay method has high specificity, high sensitivity and good repeatability. Compared with a traditional detection method, the method disclosed by the invention realizes simultaneous detection of a plurality of types of different target molecules in the same sample; the use amount of the sample is less; the method is simple and rapid to operate and the detection cost can be greatly reduced.

The invention relates to a multiplex real-time PCR kit and method for detecting respiratory pathogens and an application. The kit comprises a reagent for detecting influenza A viruses, influenza B viruses, human rhinoviruses and human metapneumoviruses; the reagent comprises a probe for detecting the viruses, a primer pair for specifically amplifying corresponding virus target genes, a reaction buffer solution, an enzyme mixture, a positive control and a negative control; and a detection channel of the influenza A viruses is FAM, a detection channel of the influenza B viruses is VIC, a detection channel of the human rhinoviruses is Texas Red, and a detection channel of the human metapneumoviruses is Cy5. The kit disclosed by the invention can be used for simultaneously detecting the four kinds of viruses, simplifies the operation process, shortens the detection time, improves the detection flux and reduces the detection cost while having high sensitivity and specificity, and can be universally applied to various fluorescent quantitative PCR instruments.

The invention belongs to the technical field of biomedicine and relates to an application of TFF2 protein in preparation of a medicine for treating and preventing lung / bronchial acute inflammation diseases. In the invention, the significantly different gene expression of the lung tissues of mice infected by severe flu and mild flu are analyzed and screened by a transcriptome chip, and the screened TFF2 is a protective factor; and according to in-vitro synthesis of TFF2 protein, the survival rate of the flu-infected mice can be remarkably increased by dropwise adding the TFF2 protein while the weight loss of mice is reduced. Further study shows that the TFF2 does not influence the flu virus replication but can reduce the secretion of inflammatory factors, promote the repair of the pulmonary mucosa and reduce the damage of lung tissues. Since the mechanism of other respiratory pathogens or physical and chemical factors causing respiratory tissue damage is similar to that of flu virus infection and the protection of TFF2 on flu virus infection is not specific protection but broad-spectrum protection, the TFF2 plays an important protection role in an acute inflammation damage model induced by respiratory pathogens or other factors.

The invention relates to a composition for detecting 23 respiratory pathogens. The composition comprises a primer composition consisting of the sequences shown by SEQ ID NO. 1-SEQ ID NO. 48 and a probe composition consisting of the sequences shown by SEQ ID NO. 49-SEQ ID NO. 72. The invention also relates to a kit for detecting 23 respiratory pathogens and a detection method of the kit. The kit comprises a first reaction solution, a second reaction solution, a first hybridization solution and a second hybridization solution containing the primers and probes respectively; the kit also comprises a mixed enzyme, a streptavidin-phycoerythrin solution, a negative reference, a positive reference and an interior label. The kit and the detection method provided by the invention integrate the functions of RT-PCR, full-automatic liquid-phase chip hybridization detection, data analysis and the like, and also have the advantages of high throughput, multiple detection target spots, high degree of automation, easiness in operation, short time consumption among the inventions of the same kind of liquid-phase chip technologies, etc.; moreover, the kit and the detection method provide important information for addressing the respiratory tract infection epidemic in time, and are of great significance to the prevention and control of respiratory pathogens.

The invention discloses a rapid fluorescent PCR detection kit for respiratory tract pathogens and a primer probe combination thereof. The kit comprises an amplification chip and a control reagent. Thecontrol reagent comprises a negative control and a positive control. The detection reagent comprises RT-PCR reaction solution consisting of Buffer, 2.0 mM dNTPs, 1 U / [mu]m L Taq DNA polymerase, 2 U / [mu]m L M-MLV reverse transcriptase, 0.3 U / [mu]m L RRI and 16 primer probes for detection of 9 respiratory pathogens. Sixteen types of primer probe mixtures of nine respiratory pathogens are pre-loadedinto a 16-channel microfluidic amplification tube by the method of reagent freeze-thawing intervention, Combined with the rapid microfluidic real-time fluorescent PCR amplification instrument, 16 types of infectious diseases associated with respiratory tract fever symptoms can be screened at one time.

The invention discloses a kit for synchronous detection of twenty three respiratory pathogens and a detection method thereof. The kit comprises: a transcription and amplification primer, 2.5 x Reaction MIX, DEPC water, a hot-start tap enzyme and a reverse transcriptase, wherein the transcription and amplification primer includes primer sequences of twenty three respiratory pathogens including 17 RNA viruses, 2 DNA viruses and 4 respiratory tract pathogenic bacteria, and the gene sequences are as shown in SEQ ID NO.1-NO.44. After pathogen samples are extracted, transcription and amplification are completed through single-tube reaction of the kit, capillary electrophoresis detection is performed, and pathogen categories are determined through software analysis of results. The technical scheme has the advantages of strong specificity, high sensitivity, fast detection speed, completion of transcription amplification by a single tube, and simple and convenient operation.

The invention provides a kit for respiratory pathogen multiple nucleic acid detection. In specific, a multiple respiratory pathogen PCR amplification system is designed and verified with experiments to obtain a multiple fluorescence PCR amplification system for detecting a plurality of respiratory pathogens, and 9 respiratory pathogens can be detected simultaneously. The kit has high sensitivity and specificity, and can achieve rapid detection and analysis on multiple respiratory pathogens in nasopharyngeal swabs, alveolar fluid, sputum and other samples.

The invention provides a respiratory tract infection multiple detection reagent kit and detection method. Particularly, a multiplex respiratory tract pathogen PCR amplification system is designed and subjected to experimental verification, so that a multiplex fluorescent PCR amplification system for detecting various respiratory tract pathogens is obtained, and a single system can detect 3 kinds of respiratory tract pathogens at the same time. The reagent kit has high sensitivity and specificity. Through the reagent kit, quick detection and analysis of multiplex respiratory tract pathogens in samples of nasopharyngeal swabs, bronchoalveolar lavage fluid, phlegm and the like can be realized.

The invention discloses a primer group, a kit and a method for detecting respiratory pathogens and relates to the technical field of respiratory pathogen detection. The primer group for detecting therespiratory pathogens comprises, such as, one or a combination of several of a first primer to a thirteenth primer. The primer group can realize detection of common respiratory pathogens such as a respiratory syncytial virus, an adenovirus, a human metapneumovirus, a rhinovirus / enterovirus, a parainfluenza virus, a coronavirus, a bocavirus, an influenza virus A, an influenza virus B, bordetella pertussis, bordetella parapertussis, mycoplasma pneumoniae and chlamydia pneumoniae and has the characteristics of relatively high sensitivity and specificity, low cost, convenience in detection, relatively good repeatability and the like.

A non-invasive method for rapidly determining the specific presence or absence of respiratory pathogens and chemical entities.

The invention discloses a kit for detecting respiratory adenoviruses, streptococcus pneumoniae and bordetella pertussis based on a micro-fluidic chip and a use method of the kit. The kit comprises a multi-flux micro-fluidic nucleic acid detection chip for actively controlling flow paths, a pre-filled dry powder detection reagent and a positive quality control product, wherein the pre-filled dry powder detection reagent contains primers with specific conserved sequences for the respiratory adenoviruses, the streptococcus pneumoniae and the bordetella pertussis and a TaqMan fluorescent probe. The kit disclosed by the invention adopts a micro-fluidic chip technology, all operation processes are completed by instruments after application of a sample, the operation is simple and convenient, thespeed is high, the detection can be completed within 30-60 min, and pollution can be avoided; the fluorescent PCR technology of the TaqMan probe is adopted to detect the respiratory adenoviruses, thestreptococcus pneumoniae and the bordetella pertussis; the sequences of the designed primers and probe are very conserved in genes of the respiratory adenoviruses, the streptococcus pneumoniae and the bordetella pertussis, and the designed primers and probe have high specificity; and detection results can be obtained within 2h, and the sensitivity can reach 10 copies / mu L.