The invention provides a fluorescently
label amplification kit for simultaneously amplifying human 27
STR loci. The fluorescently
label amplification kit comprises 24 autosomal
STR loci, one Y-
chromosome STR locus, and two specific amplified primer pairs for gender-identified
STR loci. The loci are D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, D2S1338, D6S1043,D22S1045, D19S433, D1S1656, D12S391, D10S1248, D2S441, vWA, D8S1179, TPOX , FGA, SE33, Penta D, DYS391, Y-
INDEL and
Amelogenin. The loci contain 20 core loci and four preferred loci as prescribed by the Ministry of
Public Security, all sites of mainstream kits currently on the market are covered, the risk of incorrect gender identification due to the deletion of the Y-
chromosome can be effectivelyprevented, and the advantages of high discrimination power and
high probability of paternity exclusion are achieved.