Kit for respiratory pathogen multiple nucleic acid detection

Abstract

The invention provides a kit for respiratory pathogen multiple nucleic acid detection. In specific, a multiple respiratory pathogen PCR amplification system is designed and verified with experiments to obtain a multiple fluorescence PCR amplification system for detecting a plurality of respiratory pathogens, and 9 respiratory pathogens can be detected simultaneously. The kit has high sensitivity and specificity, and can achieve rapid detection and analysis on multiple respiratory pathogens in nasopharyngeal swabs, alveolar fluid, sputum and other samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a multiple nucleic acid detection kit for respiratory pathogens. Background technique [0002] Acute respiratory tract infection (ARTI) is one of the most common infectious diseases in humans worldwide, and its incidence rate ranks first among acute infectious diseases. On average, lower respiratory tract infection causes about 4000000 patients to die every year. Globally, respiratory infections are the leading cause of death in children under 5 years of age. ARTI is generally caused by bacteria and viruses, about 90% of which are caused by respiratory viruses, but death and severe cases are mainly caused by bacterial infections. In the face of the current complex situation of prevention and control of respiratory infectious diseases, strengthening the monitoring of epidemic information of infectious epidemic strains can provide a basis for formulating prevent...

AI Technical Summary

Problems solved by technology

However, this method is complex, cumbersome to operate, long cycle, low sensitivity, relatively high requirements for samples, and prone to false negative results

[0006] Serological testing is based on the principle of antigen-antibody reaction. The detection cycle is short, but the specificity and sensitivity are low, and false negative and false positive results are prone to occur, which has certain limitations.

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