Multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens

Abstract

The invention relates to the field of medical examination, in particular to a multi-connected detection reagent card adopting immunochromatography and used for respiratory pathogens. The multi-connected detection reagent card comprises multiple sample pads and multiple nitrocellulose membranes connected with absorbent paper, wherein each sample pad is covered with multiple fluorescent microspheres, and the surface of each fluorescent microsphere is covered with an antibody of one respiratory pathogen; each nitrocellulose membrane connected with the absorbent paper is provided with at least two detection lines, and the detection lines are arranged at the nitrocellulose membrane; each detection line is fixedly covered with an antibody of one respiratory pathogen, and the antibody is in a pairing relation with the antibody, covering the surface of one fluorescent microsphere covering the corresponding sample pad, of one respiratory pathogen. The reagent card is simple, convenient and rapid to operate; different specific test strips can be formed through matching of different nitrocellulose membranes connected with the absorbent paper and the sample pads, different test strips are matched in a mixed manner and put in card paper according to demands, the detection flexibility and the customization property are high, and the detection cost is saved.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a respiratory pathogen immunochromatography multiple detection reagent card. Background technique [0002] Respiratory tract infection refers to pathogens infecting the respiratory system such as the nasal cavity, throat, trachea and bronchi of the human body. Divided into upper respiratory tract infection and lower respiratory tract infection, the common upper respiratory tract infection is acute upper respiratory tract infection (acute upper respiratory tract infection), which refers to the general term for acute inflammation of the nasal cavity, pharynx or larynx, and is the most common infectious disease of the respiratory tract. The common cause is a virus, and a few are caused by bacteria. Patients regardless of age, gender, occupation and region. Not only is it highly contagious, but it can also cause serious complications. Lower respiratory tract infection is the most co...

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Problems solved by technology

[0004] Although there are various detection methods for respiratory pathogens, it is still very difficult to confirm the pathogen of infection

Bacterial pathogen isolation and virus culture tissue cell culture method used to be used as the "gold standard", but it is difficult to achieve due to the disadvantages of complicated operation, long culture time, technical difficulty and low positive rate, and has been adopted in the past 10 years. Molecular approach replaced by

With the rapid development of rapid diagnostic technology, molecular detection technology is becoming more and more perfect, and has been used as a new standard for the detection of respiratory pathogens, but it cannot be popularized due to its high requirements on laboratory conditions, and there are few commercial products, and no series have been formed. , most pathogens are not yet detectable

In recent years, the development of immune technology, especially the wide application of immune labeling technology, such as direct and indirect immunofluorescence, anti-alkaline phosphatase bridge enzyme labeling method and other labeling technologies, has enabled the development of methods for the detection of respiratory pathogens using immunofluorescence technology However, this technology still has many shortcomings: the problem of non-specific staining has not been completely solved, the objectivity of the result judgment is insufficient, the detection and analysis technical procedures are complicated, the professional technical level of the operators is very high, and the joint detection of multiple pathogens is time-consuming long time, cumbersome operation

At present, the indirect and direct immunofluorescence antibody method (IFA / DFA) can be combined into the form of multiple inspections. There is an agent in China to import the immunofluorescence detection reagents from VIRCELL, Spain, which can simultaneously detect 9 respiratory pathogen antibodies for clinical auxiliary diagnosis. , but this method still has defects such as cumbersome operation steps, high professional level and clinical experience requirements for operators, and the need for precise operation electron microscope observation.

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