151results about How to "Get test results quickly" patented technology

The invention discloses a liquid reagent for determining N-acetyl-beta-D-glucosaminidase, which takes 5-(4-(3-methoxyl-phenylmethene-)-rhodanine-3-qmmonium acetate-N-acetylamino-beta-D-glucoside as substrate and is prepared by adding proper buffer solution, surfactant, preservative, stabilizer and the like. The liquid reagent has the advantages of large solubility of the substrate, strong stability, high detection sensitivity, convenient clinical detection, low detection cost and small outside interference for the detection result of N-acetyl-beta-D-glucosaminidase.

The invention relates to an indoor human body detection method based on an infrared array sensor. The method comprises steps that when some frame where a person exists is detected, the counting results of continuous three frames from an initial frame are compared, whether the counting results of at least two frames are the same or not is determined, if yes, the counting results of the two frames are output, otherwise, whether differences between the counting results of adjacent frames are both 1 or not is determined, and if yes, the average value of the counting results of the continuous frames is output; and temperature data of two frames are obtained, whether the counting result of the first frame is the same as the last detection result or not is determined, if yes, the output is maintained unchanged, otherwise, whether the counting result of the second frame is the same as the last detection result or not is determined, if yes, the output is maintained unchanged, otherwise, whether the counting results of the first frame and the second frame are the same or not is determined, if yes, the counting result is output as the detection result of this time, otherwise, the output is maintained unchanged. Compared with the prior art, the method is advantaged by being higher in detection precision, and capable of effectively eliminating interference and the like.

The invention relates to a method for detecting 1,5-anhydro sorbitol and a related diagnostic kit. In the method, a sample and adenosine diphosphate are reacted in the presence of adenosine diphosphate dependent hexokinase serving as a catalyst to form anhydroglucose-6-phosphoric acid and adenylic acid, the anhydroglucose-6-phosphoric acid and thio-oxidative NAD(nicotinamide adenine dinucleotide) are reacted in the presence of 1,5-AG(anhydro glucitol)-6-phosphate dehydrogenase(PDH) serving as a catalyst to form thio-reduced coenzymes and compounds of C6H11O8P, and simultaneously the generated C6H11O8P is reacted in the presence of AG-6-PDH and reduced NAD to form AG-6-P and oxidative coenzymes; and because of the cyclic amplification, the sensitivity of 6-anhydro sorbitol is improved. The invention also provides a detection kit for 1,5-anhydro sorbitol based on the method. The kit is convenient and easy to use, can quickly finish detection on a common ultraviolet / visible light analyzer or semi- / full-automatic biochemical analyzer, does not need special or additional instruments and is low in cost.

The invention provides a kit for quantitatively detecting niacin, nicotinamide and pantothenic acid in peripheral whole blood at the same time, which mainly consists of dried blood slices (Whatman protein saver 903), capillary blood collection tubes, nicotinic acid and nicotinamide and pantothenic acid vitamin standard substance and its isotope internal standard, trichloroacetic acid, and also includes dry blood spot preparation, dry blood spot pretreatment and detection steps. The nicotinic acid, nicotinamide, and pantothenic acid dry blood spot quantitative kit provided by the present invention combines dry blood spot sampling technology with high performance liquid chromatography-tandem mass spectrometry technology for the quantification of B vitamins, a minimally invasive, minimal blood collection at the same time Realize the evaluation of the nutritional status of multiple B vitamins, and realize the quantitative work of simultaneous detection of multiple vitamins under low sample volume conditions that cannot be accomplished by metabolic enzyme-dependent methods and immunoassay methods.

The invention provides a primer group for detecting seven kinds of diarrhea viruses through multiple PCR (polymerase chain reaction), wherein the primer group comprises primers shown as SEQ ID NO.1 and 3-16. The invention also provides a kit for detecting seven kinds of diarrhea viruses through multiple PCR. The kit comprises a multiple PCR primer group, revertase and DNA (deoxyribonucleic acid) polymerase. Through the technical scheme, the sensitivity, the specificity and the simplicity and convenience of the simultaneous detection on the seven kinds of diarrhea viruses are obviously improved.

The invention discloses a rapid test method for formaldehyde, which comprises the following steps: a. keeping tested spacing natural drafted, closing the windows and door for 0.5-2 hours before the test; b. making absorption liquid; c. using atmospheric sampler to sample air; d. color developing of formaldehyde absorption solution; e. color matching and reading. The invention has advantage of rapid testing about 35 minutes for all, simple testing method and rapid testing result of formaldehyde destiny by color contrasts.

The invention relates to angiotensin converting enzyme activity measuring method and its diagnosis kit. It is applied artificial synthesis substrate FAPGG to directly react with angiotensin converting enzyme to form furyl-acryloyl-phenylalanine, and to measure its activity by 340nm absorbance lowering speed. The method has high specificity, good accuracy. And it can be quickly measured by ultraviolet / visible light analyzer or semi / full automatic biochemical analysis. Its measuring cost is low; and it is convenient for generalization and application.

The invention discloses a porcine circovivus loop-mediated isothermal amplification (LAMP) kit and application thereof. The kit comprises LAMP primers, a 2* reaction buffer solution, a Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a porcine circovivus DNA template, wherein the LAMP primers comprises outer primers F3 and B3, inner primers FIP and BIP and loop primers LF and LB. Specificity detection and sensitivity detection prove that, the LAMP kit can be used for monitoring the reaction in real time, quantitatively detecting the copy number of porcine circovivuses and quickly and accurately obtaining the detection result, and brings convenience for simply, quickly and reliably detecting the porcine circovivuses.

The invention discloses a method for detecting subsurface cracks of a casting blank. The method includes the steps of: polishing away surface scale on a part to be detected of a casting blank to form a detection surface and to ensure the smoothness of the surface to be detected, and cleaning the surface to be detected; spraying a penetrating agent on the surface to be detected, wherein if the casting blank has micro cracks or cracks, the penetrating agent penetrates into the depths of the micro cracks or cracks by using the capillarity principle; cleaning the penetrating agent on the surface to be detected, spraying a photographic developer on the surface of the surface to be detected, and expressing the penetrating agent in the micro crack or cracks on the photographic developer under the effect of the capillary principle; if the casting blank has micro cracks or cracks, expressing the stripes according to the size and length of the cracks on the surface to be detected, wherein if no stripes appear, then the casting blank has no cracks or cracks. The method provided by the invention can improve the detection accuracy on surface quality of the casting blank, and avoid the influences on the subsequent production due to undetected defects of microscopic subsurface micro cracks.

The invention provides a primer group for detecting diarrhea-causing parasite genes through the multi-PCR technology. The primer group comprises primers shown as SEQ ID NO.1 and SEQ ID 3-18. The invention further provides a detection method and a kit for detecting the diarrhea-causing parasite genes through the multi-PCR technology. After the technical scheme is adopted, a complete technical means is provided for the rapid and accurate screening of the diarrhea-causing parasite genes, the rapid screening and identification for the diarrhea-causing parasites after the food poisoning event are realized, and a reliable basis is provided for reasonably and properly disposing the diseases.

The invention provides a primer set for detecting bacillus cereus virulence genes by means of a multiplex-PCR. The primer set comprises primers shown in SEQ ID NO.1 and 3-24. The invention further provides a detection method and kit for detecting bacillus cereus virulence genes by means of the multiplex-PCR. According to the technical scheme, the perfect technological means is provided for rapid and accurate screening of the bacillus cereus virulence genes, rapid screening and identification of pathogenicity bacillus cereus after a food poisoning event and outbreak of an epidemic situation can be achieved, and the reliable basis is provided for reasonable and appropriate epidemic treatment.

The invention discloses a contraband detector and a contraband detection method. The contraband detector comprises a signal generator, a circulator, an antenna, a wave detector, a filter, an amplifier, an analog-digital converter and a processor, wherein the signal generator, the circulator, the wave detector, the filter, the amplifier, the analog-digital converter and the processor are sequentially connected; and the antenna is connected with the circulator. According to the contraband detector, a millimeter wave source is adopted as a signal source of a to-be-detected sample, the material type of a to-be-detected target is determined in an inversion manner through collecting and processing an electromagnetic signal with an eigen value in an interacting echo signal of a millimeter wave and the to-be-detected sample, and whether the to-be-detected sample is a suspected object or not is further judged. The contraband detector has the advantages of being simple in structure and convenient to carry, and can be widely applied to transportation hubs such as an immigration inspection port, a railway station and an airport.

The invention discloses a kit for rapidly detecting five kinds of diarrheogenic Escherichia coli through multiple qPCR (quantitative Polymerase Chain Reaction). The kit comprises primer and probe groups capable of respectively performing specific amplification on eae genes of EPEC or EHEC, escV genes of EPEC or EHEC, bfpB genes of EPEC, stx1 or stx2 genes of EHEC lt, sth or stp genes of ETEC, aggR, astA or pic genes of EAEC and internal quality control IAC genes. Moreover, the invention further discloses a method and application for rapidly detecting the five kinds of diarrheogenic Escherichiacoli by using the kit. A method for simultaneously detecting the five kinds of diarrheogenic Escherichia coli in foods in the same reaction tube by virtue of fourteen real-time fluorescence quantitative PCR is realized, all characteristic genes in the national standard of the five DEC (diarrheogenic Escherichia coli) can be totally detected, and phenomena such as missing detection and false negative are avoided. The kit has the advantages of being short in detection time, simple and convenient to operate, easily decipherable in results, high in sensitivity, excellent in specificity and the like.

The invention discloses a steel structure corrosion identification method based on a convolutional neural network. The steel structure corrosion identification method comprises the steps: 1, collecting pictures of a steel structure, preprocessing the pictures, and dividing a training set and a test set; 2, designing a convolutional neural network structure; 3, performing hyper-parameter optimization and model training through cross validation; and 4, inputting the to-be-identified pictures of the steel structure into the model obtained in the step 3 to obtain a rust identification result. According to the steel structure corrosion identification method, the convolutional neural network is utilized to realize automatic extraction of the structure corrosion characteristics; complex and tedious characteristic design work is avoided; the steel structure corrosion identification efficiency is improved; accurate identification of the steel structure corrosion is achieved; an objective identification result can be provided; and a new solution is provided for identification of the steel structure corrosion.

The invention relates to the field of visual detection, in particular to detection equipment based on a visual detection system and a use method. The detection equipment based on the visual detection system comprises a working table plate, and a feeding area, a detection area and a receiving area which are arranged on the working table plate, and the feeding area and the receiving area are each provided with a bearing piece, an up-down driving piece, a linear driving piece, a tray supporting mechanism and a mechanical arm. The detection area is provided with a carrying mechanism, a first detection mechanism, a second detection mechanism, a third detection mechanism, a first transfer mechanism and a second transfer mechanism. The use method of the detection equipment based on the visual detection system comprises the steps of feeding, detecting and receiving. Through the arrangement of the feeding area, the detection area and the material receiving area, detection of all detection items of materials can be completed through a one-time continuous detection process; and comprehensive detection results of the materials can be quickly obtained, and unqualified materials can be screened out. During detection, the automation degree is high, circulation steps are few, and the detection efficiency can be improved.

The invention relates to enzyme method measuring sodium ion content method and its diagnosis kit. It is adopted galactosidase reaction colorimetry to use sodium ion to activate beta-galactosidase enzymolysis o-nitrophenol group-beta-D- pyranoside galactose to make 405nm dominant wavelength absorbance rise. Its absorbance amplitude of fluctuation is proportional to sample sodium ion. The method has high specificity, good accuracy. The diagnosis kit can be made into bi-agent to reduce cross influence. The method can be quickly measured by ultraviolet / visible light analyzer or semi / full automatic biochemical analysis. Its measuring cost is low; and it is convenient for generalization and application.

The invention discloses a micro dynamic probing instrument and a test method . The dynamic probing instrument comprises a probe, a probing rod, a hammer base, a guide rod, a replaceable drop hammer and a cover piece; the method comprises following steps: a test mold is used for preparing a sample, the surface of the sample is leveled, and the sample height is recorded; according to the hardness degree of the tested sample, the proper drop hammer is selected and is loaded in the probing rod; a round conical head is aligned with the surface of a soil sample to be detected, and it is ensured that the probing rod is perpendicular to the surface of the soil sample; the drop hammer is lifted to an upper baffle, and is loosened so as to freely drop; when the probing rod enters the sample by 20 mm, the probing rod is taken out, the knocking number of the drop hammer at the moment is recorded, the test is finished, and instrument devices are arranged; a parallel test is carried out; test data are arranged and analyzed. The dynamic probing instrument is small in size, and convenient to carry, operation is easy, a test result can be rapidly obtained, under the site test condition, multiple sets of tests can be achieved, the test error is reduced, and the test precision is improved.

The invention relates to nucleic acid detection, in particular to a nucleic acid detection analyzer and a detection method thereof.The nucleic acid detection analyzer comprises a rack, a temperature control device, a fluorescence detection device, a heat cover device and a reagent tube, the rack comprises a sample adding table, and an insertion hole is formed in the sample adding table; the temperature control device and the fluorescence detection device are mounted between the bottom plate and the sample adding table; the cover heating device is mounted on the rack; the reagent tube is arranged on the insertion hole and is covered by the hot cover device; the position of the reagent tube is matched with the position of the temperature control device; and the detection position of the fluorescence detection device is opposite to the reagent tube. The method is multiple in scene, low in operation requirement and suitable for popularization in price.

A method for determining concentration of homotype cysteine includes synthesizing cystathionine B synthase, homotype cysteine and serine to be cystathionine; cracking cystathionine by cystathionine B lyase to change it back to be homotype cysteine and to generate ammonia and pyruvic acid; using some chemicals to act on ammonia to let reduction coenzyme be oxidized to be oxidation coenzyme for determining out absorbance dropping speed of reduction coenzyme at 340nm then estimating out concentration size of homotyp cysteine. Its diagnosis kit can be prepared in double dosages for decreasing cross influence of various compositions.

The invention relates to a kind of enzyme colorimetric method and a method of the coupling method measuring the density of citric acid and a diagnosis reagent box applying the citrate synthetase and coupling malic dehydrogenase enzymatic reaction continues monitoring method / ratio colorimetric method. The reaction of citrate synthetase enzymolysis citric acid generates oxaloacetic acid and then through the effect of coupling malic dehydrogenase at last oxidizes the reduced coenzyme (there is a absorption peak at the site of 340nm) becoming the coenzyme (there is a absorption peak at the site of 340nm) so we can assay the degree / speed of the fall-way absorbance at the place of 340nm. It can measure and calculate the density of citric acid by measuring the degree / speed of the fall-way absorbance at the place of 340nm. The method has high specificity and it is not polluted by the endogenous and exogenous object and the test result is precise and accurate. The invention can get the array result by the ultraviolet / visible light analytic instrument so it is convenience to extend and apply.

The invention discloses a low-speed denial of service attack detection method based on a shared neighbor density cluster and an outlier factor (SNN-LOF) algorithm, and belongs to the field of networksecurity. The method comprises the following steps of: acquiring flow data information in a key route within a period of time by setting a fixed sampling time interval; And segmenting the flow data toform a plurality of detection units, and counting discrete attributes of the TCP flow and the total flow in each detection unit to form a detection data sample; And constructing a shared neighbor similarity matrix for the detection samples, and performing density clustering by taking the shared neighbor similarity matrix as density measurement to form divided clusters; And introducing a known data sample, calculating an outlier factor of the introduced data by utilizing an outlier factor algorithm, classifying the cluster according to a judgment criterion, and judging whether a slow denial ofservice attack exists or not. The detection method based on SNN-LOF algorithm proposed by the invention can detect slow denial of service attacks accurately, quickly and efficiently..

The embodiment of the invention provides a method and device for testing a user interface, electronic equipment and a readable storage medium, and aims to improve the detection efficiency. The methodcomprises: for a target interface module on a reference user interface, obtaining a view hierarchy tree of the target interface module, wherein all nodes included in the view hierarchy tree correspondto all interface elements of the target interface module respectively; determining a plurality of target interface elements from the view hierarchy tree; based on the plurality of target interface elements, executing snapshot operation to obtain a reference image; obtaining a plurality of to-be-tested interface elements from a to-be-tested user interface according to the plurality of target interface elements, the plurality of to-be-tested interface elements being in one-to-one correspondence with the plurality of target interface elements; based on the plurality of to-be-tested interface elements, executing snapshot operation to obtain a test chart; and performing similarity comparison on the test image and the reference image to obtain a test result.

The invention relates to a portable physiological index detector based on a micro fluidic paper chip and a detection method thereof. The portable physiological index detector comprises a detected physiological index sample, a micro fluidic paper chip, a voltage follower module, a voltage amplifier module, an A / D transition module, and a single-chip microcomputer module which are connected in order, the single-chip microcomputer module is respectively connected with a button module, a display module and a voice module, which can effectively solves the problems of measurement error due to detection of the physiological index by the micro fluidic paper chip. The portable physiological index detector has the advantages of ingenious conception, humanized design and easy carrying, the novel micro fluidic paper chip is used for detecting the uric acid concentration, a potential signal generated after a biochemical reaction is subjected to voltage following, voltage amplification, and AD transition to a digital electric signal, the digital electric signal is sent to a STM32-series single-chip microcomputer or intelligent analysis and processing, detection result can be rapidly obtained and displayed on a screen, relative high concentration, relative low concentration or normal measurement result can be prompted by the system by comparing with a health standard, and the portable physiological index detector has market development potential.

The invention discloses a method and a device for rapidly detecting raw milk bacteria. The method comprises the following steps of step1, placing the milk to be detected in a centrifuge tube with a precipitator, and vibrating the centrifuge tube for 3-8 seconds; step 2, placing the centrifuge tube into a centrifugal machine for centrifugal treatment; step 3, absorbing a clear solution of a liquid middle layer in the centrifuge tube by using an injector, and injecting the clear solution into a sample pool of a detection device; step 4, starting a high-speed channel pump, and enabling a sample liquid in the sample pool to enter a high-speed channel; step 5, stopping the high-speed channel pump, starting a sampling pump, and enabling the sample liquid to enter a sample flowing groove; step 6, carrying out image collection on the sample liquid by using a microscopy camera, transferring a collected image to an accelerator; and the step 7, recognizing the image through the accelerator and the calculating the bacterial content in the sample liquid. According to the technical scheme, a condition that the raw milk bacteria are rapidly and accurately detected is realized, the detection precision is high, and the scheme is suitable for milk production processing enterprises and detection departments.

A test strip for detecting human ABO blood types, a preparation method, a detection method and an application, which belong to the field of biological medicines. The test strip includes a polyester board, and absorbent paper bonded to the upper edge of the polyester board; and a sample pad and a nitrocellulose membrane pre-coated with A resistance, B resistance and RBC resistance are sequentiallylaid on the upper surface of the polyester board in a chromatographic direction, the upper edge of the nitrocellulose membrane is overlapped with the lower edge of the absorbent paper, and the samplepad is paved with a hemolytic agent and a cell membrane fluorescent dye. The invention provides a preparation method for the test strip for detecting the human ABO blood types, and the test strip in which a blood type detection supporting reagent does not low temperature preservation and which is simple in operation and high in sensitivity can be prepared through the preparation method. Moreover,the test strip for detecting the human ABO blood types is provided, and the test strip can complete joint detection of multiple antigen projects in a short time, is simple and quick in operation, is low in professional technical level requirement for operation personnel, is suitable for clinical detection, and is quick in obtainment of a test result.

The invention relates to measuring method for carbon dioxide content and diagnosing kit. The invention directly uses carbon dioxide( bicarbonate radical) and pyruvic acid to produce malic acid by decarboxylation of malic dehydrogenase and oxidizes reduced coenzyme into oxidized type coenzyme, measuring the decent rate of dominant wavelength 340nm absorbance could quantitatively response the carbon dioxide content in sample, thus, the strength of bicarbonate radical could be extrapolated. The method has high specificity, has no pollution from inside and outside material, and accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet / visible light, analysis or semi-transfer / full automatically biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.

The active feature inspection for acetylglucosamidase and its diagnostic agent box uses its fostering of continuous monitoring method, enzymolysising dual chlorineor chlorine acetylglucosamidase reaction, through the absorbance elevating speed of the 405nm, inspecting its active feature. The agent box comprises the bumper solution, synthesized nitrobenzene betaacetylglucosamidase and stabilizer to make dual agent by the diagnostic agent box to reduce cross impact of all kinds of components. Through visible light analyzer, the result is precise, free from outside contamination, easy for promotion.

The invention provides a kit for quantitatively detecting cassava components based on micro-drop digital PCR and an application thereof, and belongs to the technical field of molecular biology. The kit comprises an upstream primer of an mSOD2 gene, a downstream primer of the mSOD2 gene and a probe of the mSOD2 gene. The application of the kit in the quantitative detection of the cassava componentin a sample is further provided. The method includes utilizing a digital PCR system to detect a cassava-specific single-copy species gene mSOD2 fluorescence signal to obtain the measured cassava-specific species gene mSOD2 copy number concentration, and the content of the cassava component is calculated according to the linear relationship between the copy number concentration (copy number / [mu]L)and the cassava mass (mg). The kit can specifically and quantitatively detect the cassava component, and has the advantages of being simple, rapid and high in detection sensitivity.

The invention provides a test method, device and system for a hybrid mobile application. A command to be tested is sent to a second electronic device so as to acquire an application code update command, a source code acquisition command is sent to the second electronic device, the second electronic device can timely acquire a modified newest application source code, a test tool corresponding to the modified newest application source code is taken from a first electronic device to test the newest application source code, and a test result is rapidly acquired after the source code of the application to be tested is modified. It is thus clear that the newest application source code of the application to be tested is acquired from the second electronic device in real time, the preset test toolis taken to test the newest application source code, the test time can be effectively shortened, and the test efficiency is improved.