Porcine circovivus loop-mediated isothermal amplification kit and application thereof

Abstract

The invention discloses a porcine circovivus loop-mediated isothermal amplification (LAMP) kit and application thereof. The kit comprises LAMP primers, a 2* reaction buffer solution, a Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a porcine circovivus DNA template, wherein the LAMP primers comprises outer primers F3 and B3, inner primers FIP and BIP and loop primers LF and LB. Specificity detection and sensitivity detection prove that, the LAMP kit can be used for monitoring the reaction in real time, quantitatively detecting the copy number of porcine circovivuses and quickly and accurately obtaining the detection result, and brings convenience for simply, quickly and reliably detecting the porcine circovivuses.

Description

technical field [0001] The invention relates to the technical field of microbial detection, in particular to a fast, visualized and real-time quantitative detection of porcine circovirus ring-mediated isothermal amplification kit and its application. Background technique [0002] Circoviruses (PCVs) belong to the Circoviridae family and are the smallest known vertebrate viruses. Pigs are the main host of circovirus, and pigs of all ages have strong susceptibility to circovirus. Pigs infected during the embryonic period or early postnatal period often develop the disease after weaning, generally concentrated at the age of 5 to 18 weeks, especially at the age of 6 to 12 weeks. Pregnant sows infected with circovirus lead to reproductive impairment and can be vertically transmitted to piglets through the placenta. Infected pigs can excrete the virus from nasal fluid, feces and other excreta, and cause infection through the digestive tract and respiratory tract. [0003] A ne...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems such as difficult, time-consuming and expensive instruments for detecting porcine circovirus at the grassroots level, and to provide a simple, fast and accurate test kit for detecting porcine circovirus at the grassroots level. The technical solution is: a porcine circovirus ring-mediated isothermal amplification kit, which includes LAMP primers, 2× reaction buffer, Bst DNA polymerase, fluorescent visual detection reagents, ultrapure water and porcine circovirus DNA template; the LAMP primers include outer primers F3 (SEQ ID NO: 1) and B3 (SEQ ID NO: 2), inner primers FIP (SEQ ID NO: 3) and BIP (SEQ ID NO: 4) and loop Primers LF (SEQ ID NO: 5) and LB (SEQ ID NO: 6);

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