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54results about How to "Save testing cost" patented technology

Detection device for monitoring water quality on line and water quality on-line monitoring method

The invention provides a detection device for monitoring water quality on line. The detection device comprises a sampling device, a microorganism fixing device, and an electrochemical detection device wherein a sample inlet of the microorganism fixing device is connected with a sample outlet of the sampling device; the microorganism fixing device is a microbial film reactor; the microbial film reactor comprises a container and a microbial film arranged in the container; the microbial film comprises a microbial carrier and a microorganism attached to the microbial carrier; the microbial film reactor is placed in a thermostat; a sample inlet of the electrochemical detection device is connected with a sample outlet of the microbial film reactor; the electrochemical detection device comprises a detection pool, an electrode system, an electrochemical work station and a program control device; the electrode system is arranged in the detection pool; the electrochemical work station is connected with the electrode system; and the program control device is used for controlling the electrochemical work station. The invention further provides a water quality on-line monitoring method. When the on-line monitoring device detects the biochemical oxygen demand and the toxicity of the water sample, the result is accurate.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI

Method for simultaneously detecting twelve kinds of common respiratory viruses

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.
Owner:FUJIAN INT TRAVEL HEALTH CARE CENT +1

Website security detection method and device

The invention discloses a website security detection method and device. The website security detection method comprises the steps that a website is obtained; website programs used by the website are identified based on website fingerprint information of the website, and loophole detection is conducted on the website according to the website programs; lastly, a detection result is returned. By means of the website security detection method and device, the cost of the loophole detection on the website can be reduced obviously, the loophole detection speed can be increased, and the rate of missing report can be decreased.
Owner:ALIBABA GRP HLDG LTD

Manufacturing method of back drill holes on PCB (Printed Circuit Board)

The invention discloses a manufacturing method of back drill holes on a PCB (Printed Circuit Board). The manufacturing method comprises the following steps of: in a manufacturing process of the PCB, dividing all layers of the PCB into layers for forming a metalized hole layer and layers for forming a non-metalized hole layer according to layered distribution of metalized holes and non-metalized holes of the back drill holes on the PCB to be manufactured; respectively manufacturing and processing all the layers forming the metalized hole layer and all the layers forming the non-metalized hole layer to form a metalized hole layer with the metalized holes and a non-metalized layer with the non-metalized holes; and laminating the metalized hole layer with the metalized holes and the non-metalized layer with the non-metalized holes through a prepreg with low fluidity to form the PCB with the back drill holes. Therefore, according to the manufacturing method disclosed by the invention, useless hole copper can be completely eliminated, so that the back drill holes without useless hole copper residual can be manufactured on the PCB, and the signal transmission integrity of the PCB is ensured so as to adapt to development requirements of electronic products.
Owner:CHINA CIRCUIT TECH SHANTOU CORP +1

Enterobacter sakazaii colour development culture medium, detection kit and detection method

The invention provides a fluorogenic culture medium of Enterobacter sakazakii, a test kit and a test method thereof, and relates to a method for testing microbe and a composition used by the method. The fluorogenic culture medium comprises the compositions as follows: 8 to 20 grams of peptone, 4 to 7 grams of beef extract powder, 4 to 7 grams of sodium chloride, 1.0 to 2.0 grams of cholate, 12 to 20 grams of agar, 0.05 to 0.5 grams of X-a-glucopyranoside, 0.01 to 0.04 grams of Na2CO3, 0.3 to 2.0 grams of ferric ammonium citrate, and 0.3 to 2.0 grams of sodium thiosulfate. The test kit consists of the fluorogenic culture medium of Enterobacter sakazakii, an enriched liquid A ,namely buffer peptone water culture medium, and an enriched liquid B, namely modified lauryl sulfate pancreas peptone broth. The test kit is configured simply, and the test method has high sensitivity in detection, so the method is suitable for treating large flux samples.
Owner:GUANGDONG HUANKAI MICROBIAL SCI & TECH

Kit for synchronously detecting twenty-three meningitis pathogens and detection method of kit

The invention discloses a kit for synchronously detecting twenty-three meningitis pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the twelve meningitis pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-13 (sequence identifier number 1-13), and the PCR primer comprises forward and reverse PCR amplification primers of the rest eleven meningitis pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the twelve meningitis pathogens and the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 14-52. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Animal epidemic disease three-color fluorescence RT-PCR detection kit and detection method thereof

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.
Owner:NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE

Triple fluorescent quantitative PCR detection material and kit for distinguishing African swine fever virus wild strains from CD2V and/or 360-505R gene deleted strains

The invention discloses a triple fluorescent quantitative PCR detection material and kit for distinguishing African swine fever virus wild strains from CD2V and / or 360-505R gene deleted strains. The detection material comprises primers and probes, and the nucleotide sequences are shown as SEQ ID NO: 1-9. According to the invention, triple fluorescent quantitative PCR amplification is carried out on African swine fever virus P72, CD2V and 360-505R genes by utilizing three groups of primer probes, so that the detection cost and the detection time for identifying different genes are reduced; whether gene deletion exists in the strain or not can be distinguished; the detection material and kit have high specificity and sensitivity; and for traditional qPCR enabling only one gene to be amplified each time, the provided mode has the following advantages: three genes are amplified at the same time in each PCR reaction, and the cost is reduced by about 2 / 3.
Owner:SOUTH CHINA AGRI UNIV +1

Double-fluorescent PCR detection primer, probe, reaction liquid and kit capable of detecting pathogens of respiratory tract

The invention discloses a double-fluorescent PCR detection primer, a probe, reaction liquid and a kit capable of rapidly detecting 16 pathogens of the respiratory tract. The kit comprises pre-subpackaged PCR reaction liquid, RT-PCR enzyme, a positive quality control product and a negative quality control product, wherein the pre-subpackaged PCR reaction liquid is provided with a primer and a TaqMan probe for detecting at least two of the following pathogens: respiratory syncytial virus, enterovirus, coronavirus NL63, coronavirus HKU1, coronavirus 229E, coronavirus OC43, parainfluenza virus type I, parainfluenza virus type II, rhinovirus, parainfluenza virus type III, human bocavirus, human metapneumovirus, mycoplasma pneumoniae, chlamydia pneumoniae, adenovirus and legionella pneumophila. The kit is convenient to operate, and can be used for at most screening 16 syndrome pathogens of the respiratory tract within 2 hours, so that the detection time and cost are greatly saved. The kit has the greatest advantages of simplicity in operation and strong practicability, and can be easily popularized in laboratories of the ports.
Owner:SHENZHEN INT TRAVEL HEALTHCARE CENT +1

Handheld depth finder / fish detector / transducer tester

ActiveCN103760550ARealize the effect of combining two testers into onePortable and adaptableWave based measurement systemsMulti-tester circuitsLED displayControl cell
The invention relates to a handheld depth finder / a fish detector / a transducer tester. The handheld depth finder / the fish detector / the transducer tester are characterized by comprising an MCU micro control unit, an LCD display screen control circuit, a key control circuit, a charging port circuit, an ultrasonic wave receiving module, an ultrasonic wave transmitting module and an underwater acoustic transducer test module, wherein the LED display screen control circuit, the key control circuit, the charging port circuit, the ultrasonic wave receiving module, the ultrasonic wave transmitting module and the underwater acoustic transducer test module are all connected with the MCU micro control unit, and the MCU micro control unit controls other circuits and function modules. According to the technical scheme, the handheld depth finder, the fish detector and the transducer tester are ingenious in design, high in precision, stable in performance and reliability and convenient to carry and use.
Owner:南京宁禄科技有限公司

Flat panel display panel uniformity detection method and system

The invention discloses a flat panel display panel uniformity detection method and system. The detection method comprises the following steps that a frame generating unit generates a frame to be detected according to a control instruction, wherein the frame to be detected is used for being displayed on a panel to be detected; a frame obtaining unit shoots the frame which is to be detected and is displayed on the panel to be detected so that a detection frame comprising a plurality of detection areas and a plurality of contrast areas can be generated; a processing unit detects a minimum pixel value from each detection area of the detection frame, selects a pixel from one contrast area in the detection frame and takes the value of the pixel of the contrast area as a pixel reference value; a detection unit detects in the detection frame whether at least one minimum pixel value smaller than the pixel reference value exists or not, and if yes, the detection unit determines that the panel to be detected is a panel with non-uniform brightness.
Owner:TOP VICTORY INVESTMENTS

Primer composition for guiding nitroglycerin medication and healthy drinking, multiple gene detection kit and use method of kit

The invention discloses a primer composition for guiding nitroglycerin medication and healthy drinking, a multiple gene detection kit and a use method of the kit, and the kit comprises the primer composition, a PCR buffer solution and a positive reference substance, and the PCR buffer solution comprises ultrapure water, an X solution, a 10*PCR (polymerase chain reaction) buffer solution, a PCR primer, a 25mM magnesium chloride solution and DNA polymerase, the primer composition comprise two forward and reverse amplification primers of different gene types on the 2 SNP sites of genes related to the nitroglycerin medication and healthy drinking and forward and reverse amplification primers capable of reflecting internal reference, the gene sequences of the primers are represented as SEQ ID NO.1-NO.8; the use method comprises the step of acquiring a sample and extracting nucleic acid, the step of performing the PCR reaction by using extracted nucleic acid as a template, and the final step of separating the sample through capillary electrophoresis. The primer composition has the advantages of being strong in specificity, high in accuracy, high in flux, strong in reliability, low in cost and free from false negative result.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Bridge expansion joint displacement monitoring device

InactiveCN106840064AAccurate and timely detection of deformation failure problemsDiscover deformation failure problemsMeasurement devicesElectricityEngineering
The invention discloses a bridge expansion joint displacement monitoring device. There is a first beam plate on one side of the bridge expansion joint and a second beam plate on the other side, including a horizontal spring body, a microprocessor, an early warning device, and a first tension pressure sensor. , the second tensile pressure sensor, fit the first shaped steel fixed on the end face of the first beam plate, fit the second shaped steel fixed on the end face of the second beam plate, the first shaped steel is provided with a first notch groove, and the second shaped steel The section steel is provided with a second notch groove, the first tension pressure sensor is embedded in the first notch groove, the second tension pressure sensor is embedded in the second notch groove, and the first tension pressure sensor is connected to the second tension sensor through the spring body. The tension and pressure sensors are connected, and both the first tension and pressure sensors and the second tension and pressure sensors are electrically connected to the early warning device through the microprocessor. The invention realizes accurate detection of the displacement of expansion joints parallel to and perpendicular to the axis of the bridge, has high efficiency, strong real-time performance, safe and simple inspection work, reliable and comprehensive results, and greatly saves detection costs and detection procedures.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for real-time quantitative polymerase chain reaction (PCR) detection of bifidobacteria and Escherichia coli by using Taqman probes

InactiveCN102559912AKinetic Change MonitoringReveal diversityMicrobiological testing/measurementMicroorganism based processesEscherichia coliPcr method
The invention discloses a method for real-time quantitative polymerase chain reaction (PCR) detection of bifidobacteria and Escherichia coli by using Taqman probes. The method comprises the following steps of: A) extracting bacterial genome DNA of the bifidobacteria and the Escherichia coli in a sample to be detected; B) synthesizing primers and the Taqman probes according to related sequences of the bifidobacteria and the Escherichia coli; and C) ensuring that the primers and the probes obtained in the step B) and other PCR reaction reagents form a reaction system for real-time quantitative PCR by the Taqman probes. The Taqman probes are labeled by double colors, by a double absolute quantitative PCR method, two kinds of intestinal bacteria in a sample can be accurately quantified at one time, the detection cost and detection time can be saved, and errors caused by repeated detection are reduced.
Owner:SHENZHEN CHILDRENS HOSPITAL

Kit for synchronously detecting fifteen hemorrhagic fever pathogens and detection method of kit

ActiveCN103074452AMonitor reaction efficiencyEnsuring Quality JudgmentsMicrobiological testing/measurementMicroorganism based processesPositive controlReverse transcriptase
The invention discloses a kit for synchronously detecting fifteen hemorrhagic fever pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine hemorrhagic fever pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-10 (sequence identifier number 1-10), and the PCR primer comprises forward and reverse PCR amplification primers of the rest six hemorrhagic fever pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the nine hemorrhagic fever pathogens and the human RNA internal reference, and has a gene sequence show as SEQ ID NO. 10-36. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Color developing culture medium, detecting kit and detecting method for vibrio parahaemolyticus

The present invention relates to color developing culture medium, detecting kit and detection method for Vibrio parahaemolyticus, and belongs to the field of food microbe safety monitoring technology. The detecting kit consists of color developing culture medium comprising bacterial specific enzyme in 0.05-0.95 g and mixed color developing substrate Y-glucoside, enrichment liquid A of pancreatic casitone liquid culture medium and enrichment liquid B comprising sodium chloride, polymyxin and broth. The detection method includes the enrichment culture of the sample in enrichment liquid A and enrichment liquid B, the culture of the two parts of enrichment cultured liquid in the color developing culture medium, and judging whether to exist Vibrio parahaemolyticus according to whether to have smooth raised purple colony of 2-3 mm diameter. The present invention has low cost, high detection sensitivity, short detection period and other advantages.
Owner:广东实验中学 +1

Detection kit, primer and probe for simultaneously detecting and identifying classical swine fever, African swine fever and swine vesicular disease

The invention discloses a detection kit, a primer and a probe for simultaneously detecting and identifying classical swine fever, African swine fever and a swine vesicular disease. The primer and the probe are shown as a sequence table SEQ ID NO: 1 to SEQ ID NO: 16. The invention further discloses the one-step-method multi-fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit which is rapid, accurate and convenient to use and can be used for simultaneously detecting and identifying the classical swine fever, the African swine fever and the swine vesicular disease. A detection method disclosed by the invention can be used for realizing one-time sampling and one-time analysis and can realize the aim of simultaneously detecting and distinguishing three important viruses, so that the working amount and cost of detection are reduced, the detection of epidemic diseases can be finished within shortest time and the time for preventing and controlling the diseases is earned.
Owner:BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT

Multiple PCR specific primers, kit and method for detecting hereditary hearing loss gene based on high throughput sequencing technique, kit and method

The invention belongs to the field of gene detection and especially relates to multiple PCR specific primers, kit and method for detecting a hereditary hearing loss gene, based on a high throughput sequencing technique. The primers include 52 pairs of specific primers, the nucleotide sequences of which are shown as SEQ ID NO:1 to SEQ ID NO:104. The kit further comprises the specific primers, a universal primer, an enzyme mixture, a quality control substance and nuclease-less water. The invention also discloses a detection method for capturing and amplifying the hereditary hearing loss gene through once PCR amplified reaction by utilizing the primers or kit. According to the scheme provided by the invention, the detection efficiency is effectively increased, the accuracy is increased, the cost is lowered and the operation step is simplified.
Owner:广州奇辉生物科技有限公司

PCB manufacturing method for conveniently detecting back drilling hole precision

The invention relates to the field of printed circuit board manufacturing and specifically relates to a PCB manufacturing method for conveniently detecting back drilling hole precision. According to the method, an open loop conductive annular line is arranged around each back drilling position; after back drilling operation is completed, a universal meter is used for detecting whether the annular line is open or not via detection points; if the annular line is open, the annular line is broken via drilling operation; if the annular line is short circuited, the annular line is not broken via the drilling operation. Via measurement, identification marks of short circuits are written down, and the identification marks indicate numerical values of distance between back drilling holes and annular lines and numbers of layers on which the annular lines are positioned; via common add-and-subtract operation, accurate offset can be known in a visual manner, and back drilling hole precision can be read in a simple and convenient manner. When the back drilling holes are large in quantity, poor quality holes can be identified easily. Via use of the method, simple operation can be achieved, detection cost and man power cost can be greatly lowered, and working efficiency can be improved.
Owner:SHENZHEN SUNTAK MULTILAYER PCB

Turbot fluorescence labeling microsatellite sextuple PCR family tree recognizing method

The invention relates to a turbot fluorescence labeling microsatellite sextuple PCR family tree recognizing method which comprises the following steps: using three kinds of fluorescence (6FAM, HEX andNED) for pairwise labeling six pairs of primers according to the sizes of the PCR amplifying products of the primers; using primer design software for excluding the possibility that the six pairs ofprimers form dipolymer in the PCR reaction process, and combining the six pairs of primers in the same PCR reaction system which comprises ROX fluorescence molecule internal labels; adjusting the PCRreaction system, and establishing parameters which can enable the six pairs of primers to be simultaneously amplified in the same PCR reaction system and a proper PCR reaction procedure; and analyzingPCR products in six microsatellite positions of the turbot through ABI3130 or a similar gene analyzer according to the fluorescence labels. The sextuple PCR technology can improve the detection efficiency of the turbot microsatellite labeling, wherein the single parent excluding ratio is 99.63%, and the individual recognizing ratio is 99.99%; and the sextuple PCR technology can distinguish different family trees of the turbot or recognize and identify individuals and parent children.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Handheld log indicator / transducer tester

InactiveCN103760549ARealize the effect of three in onePortable and adaptableWave based measurement systemsMulti-tester circuitsComputer hardwareControl cell
The invention relates to a handheld log indicator / a transducer tester. The handheld log indicator and the transducer tester comprise an MCU micro control unit, an LCD display screen control circuit, a key control circuit, a charging port circuit, an ultrasonic receiving module, an ultrasonic transmitting module, an underwater acoustic transducer test module, an NMEA detection module and an RS232 communication conversion module, wherein the LCD display screen control circuit, the key control circuit, the charging port circuit, the ultrasonic receiving module, the ultrasonic transmitting module, the underwater acoustic transducer test module, the NMEA detection module and the RS232 communication conversion module are all connected with the MCU micro control unit, and other circuits and function modules are controlled through the MCU micro control unit. According to the technical scheme, the handheld log indicator and the transducer tester are ingenious in design, high in precision, stable in performance and reliability and convenient to carry and use.
Owner:南京宁禄科技有限公司

Gait analysis method for assisting in screening meniscus injuries

InactiveCN106650195AAccurate and Rapid Assisted ScreeningAvoid non-invasive diagnosticsHealth-index calculationMedical automated diagnosisMeniscal injuryKinematics
The invention discloses a gait analysis method for assisting in screening meniscus injuries. The gait analysis method includes the steps that gait feature data of meniscus injury patients and healthy able-bodied persons is collected and subjected to neural network modeling and training; proper gait features are selected through kinematic and dynamic analysis of body gait, and gait dynamic knowledge is obtained; then the gait feature data of the meniscus injury patients serves as a test set. In this way, screening is accurately and rapidly assisted, it is avoided that non-invasive diagnosis is carried out under MRI and an arthroscopy, the accuracy of preoperative diagnosis is greatly improved, and the detection cost and the detection time are saved. The gait analysis method for assisting in screening meniscus injuries can be widely applied to the field of medical treatment.
Owner:张余

Method for detecting animal pathogenic microorganisms and special protein chip thereof

The invention belongs to the field of biomedicine, providing a method for detecting animal pathogenic microorganisms and a special protein chip thereof. The detecting method essentially comprises the following steps of: a. preparing the protein chip, selecting a plurality of different groups of specific antigen of many kinds of pathogenic microorganisms by means of an experiment to be fixed on a properly modified carrier; and b. detecting: reacting blood serum of a animal to be detected with the protein chip, hybridizing with a marked secondary antibody, detecting a hybrid positive signal in a proper detection way, and obtaining a hybrid result of the blood serum and the chip by means of scanning and analyzing, wherein the blood serum infected with the different pathogenic microorganisms can show different positive signal combination modes after the reaction, so as to ensure whether the animal to be detected is infecting or is infected with the pathogenic microorganisms marked on the protein chip. The invention can simultaneously detect many kinds of the pathogenic microorganisms, improves detecting efficiency, selects many groups of different antigen with a certain specificity aiming at each pathogenic microorganism, leads the detection to have good specificity, and has good application prospect in detecting the animal pathogenic microorganisms.
Owner:INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI

Primer composition and multiple-gene detection kit for guiding administration of thiazine diuresis drugs and application method thereof

The invention discloses a primer composition and multiple-gene detection kit for guiding the administration of thiazine diuresis drugs and an application method thereof. The primer composition comprises ultrapure water, an X solution, a 10*PCR buffer solution, a PCR primer, a 25mM magnesium chloride solution, a DNA polymerase and a positive reference substance and is characterized in that the PCR primer comprises the following ten forward / reverse amplification primers and reaction reference forward / reverse amplification primers of different gene types on 15 SNP sites on the gene related to the administration of thiazine diuresis drugs, wherein the gene sequences are shown by the SEQ ID No.1 to No.46. The application method comprises the following steps: collecting the samples and extracting nucleic acid; performing a PCR reaction by taking the extracted nucleic acid as a template; and finally, performing capillary electrophoretic separation of the samples with the GeXP genetic analyzer. The primer composition has the advantages of strong specificity, high accuracy, high flux, high reliability, low cost and no false negative result.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Overall type steel-based platform static load test load piling method for civil engineering

The invention relates to an overall type steel-based platform static load test load piling method for civil engineering. The overall type steel-based platform static load test load piling method for civil engineering is characterized in that the method is performed according to the following steps that, a steel-based platform is selected, a jack is installed at the center of test points, a main beam is installed on the steel-based platform, a jack cylinder body and the main beam are effectively connected, and the steel-based platform and the main beam are effectively connected; a reference beam is installed, and loads are piled; after the weight of the piled loads reaches a regulated weight and a test instrument meets the test requirement according to check, test loading can be performed;after a test ends, parts of the loads are unloaded; and the steel-based platform, the main beam and part of the loads are transferred to a next test point, the above steps are repeated till detectionof the test points of the whole program ends. By means of the load piling method, the large tonnage load piling potential safety hazards can be eliminated, the detection time and the detection cost are saved, a large tonnage static load experiment can be safely and conveniently implemented, integrity is good, self moving can be achieved, speed is high, cost is low, and energy conservation and environmental protection are achieved.
Owner:张宗毅

Chemical indicator monitoring disinfection effect of oxidized disinfectant and monitoring method

InactiveCN109668884AFast monitoring resultsColor indication changes significantlyMaterial analysis by observing effect on chemical indicatorDisinfectantOxygen
The invention discloses a chemical indicator monitoring the disinfection effect of an oxidized disinfectant. The chemical indicator is prepared from an indicating compound and an ink composition. Theindicating compound is triarylmethane pigment, and the mass percentage of the indicating compound to the ink composition is (6-10%) to (90-94%). The color of the triarylmethane pigment can change obviously when being exposed an oxidized disinfectant disinfection process. The method for monitoring the oxidized disinfectant disinfection process comprises the following steps that the chemical indicator and a to-be-disinfected object are placed under a same disinfection condition; color changing of the chemical indicator is observed to judge a disinfection effect, when the chemical indicator is exposed to a wet oxidized disinfectant, especially ozone, the color of the chemical indicator changes obviously; and when the chemical indicator is exposed to a dry oxidized disinfectant or oxygen, thecolor does not change. The chemical indicator provided by the invention can rapidly monitor the disinfection result, has the obvious color indicating change, and has the relatively good indicating effect.
Owner:HENAN FANGZHOU MEDICAL INSTR CO LTD

Semiconductor detection circuit and method

The invention provides a semiconductor detection circuit and method. The semiconductor detection circuit comprises at least two parallel units to be detected, a first detection end, a second detection end, at least two third detection ends corresponding to the units to be detected one to one and at least one diode. The units to be detected are used for detecting breakdown characteristics between two electrodes, and the structures of the different units to be detected are different. The second ends of every two adjacent units to be detected are connected with the second detection end, the first ends of every two adjacent units to be detected are connected through the diodes, the first end of one unit to be detected is connected with the first detection end, the anodes of all the diodes face the first detection end, and therefore all the diodes are switched on when the first detection end applies forward breakdown detection voltages. By the adoption of the semiconductor detection circuit, the unit to be detected with the worst voltage withstanding characteristic can be quickly detected, and different influences of the structures of the different units to be detected on the breakdown characteristics can be acquired.
Owner:SEMICON MFG INT (SHANGHAI) CORP

Method for determining dissolution rate of medicinal preparation containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate

The invention discloses a method for determining a dissolution rate of a medicinal preparation containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate. The method comprises the following steps of: (1) adding medicinal preparation containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate into a dissolution medium for dissolution treatment, performing sampling and filtering to obtain dissolution liquid; (2) detecting the contents of acetaminophen, dextromethorphan hydrobromide and doxylamine succinate in the dissolution liquid; and (3) and determining the dissolution rate of the medicinal preparation according to the detection result of the step (2). The method can effectively simulate the dissolution behavior in vivo in vitro, can determine the dissolution rate of the soft capsule containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate, and provides technical support for the research and development and quality control of medicaments. The method can simultaneously determine the dissolution results of the three substances under the same condition, greatly saves the detection time and the detection cost, and has good accuracy and reproducibility.
Owner:安士制药(中山)有限公司

Quality detection method for tabasheer and monkey bezoar powder

ActiveCN107817313AStrong specificityNegative interference exclusionComponent separationMedicineTest sample
The invention discloses a quality detection method for tabasheer and monkey bezoar powder. The method comprises the following steps: (1) performing primary developing on a tabasheer and monkey bezoarpowder test sample solution and a bombyx batryticatus reference crude herb solution on a thin layer plate, obtaining corresponding chromatograms through a chromatographic instrument, and comparing thechromatograms of the two solutions so as to judge whether the tabasheer and monkey bezoar powder contains a bombyx batryticatus medicinal material and eliminate negative interference; and (2) if so,performing secondary developing on the thin layer plate in the step (1), performing contrastive analysis on the chromatograms of the two solutions obtained by the secondary developing so as to determine whether the tabasheer and monkey bezoar powder contains the bombyx batryticatus. According to a thin-layer chromatography identification method, two different developing agent systems are adopted to detect different components in the bombyx batryticatus, the specificity of bombyx batryticatus detection is obviously improved, and negative interference caused by scorpio is eliminated.
Owner:GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD

Preparation method of sodium hypochlorite

The invention discloses a preparation method of sodium hypochlorite. According to the invention, a sodium hydroxide solution is utilized to absorb chlorine gas to prepare the sodium hypochlorite. The preparation method is implemented in a way that: any time before the preparation is finished, measuring the reaction tank liquid level M1 and the free alkali content N1 at that time, and determining the finishing reaction tank liquid level Mend according to the formula Mend=M1+(N1-Nend); and monitoring the reaction tank liquid level M in the subsequent reaction process, and finishing the reaction when M=Mend. According to the relationship between free alkali content difference and reaction liquid value difference of the sodium hypochlorite solution at different liquid levels in the production process, a qualified edge point of the sodium hypochlorite solution is determined by a liquid level metering method, and an empirical formula is educed; and the reaction tank liquid level is monitored to accurately judge the qualified reaction end point of the sodium hypochlorite solution, thereby effectively and conveniently controlling the production qualification rate of the sodium hypochlorite solution, greatly lowering the labor intensity of personnel, reducing the sampling frequency, relieving the problem of environmental pollution on the spot due to sampling, and avoiding the phenomenon of unqualified technical indexes due to shortness of hands or untimely inspection.
Owner:RUYUAN DONGYANGGUANG ELECTROCHEM FACTORY
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