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Quality detection method for tabasheer and monkey bezoar powder

A quality detection method and technology of Houzaosan, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to fully control the quality of Tianhuang Houzao powder, inability to effectively separate spots, negative interference, etc., and save detection costs. and detection time, improve specificity, and eliminate negative interference

Active Publication Date: 2018-03-20
GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the existing research, no animal medicinal materials such as silkworm in Tianhuang Houzao San prescription were tested, and there was a gap in the detection of animal medicinal materials, and the quality of Tianhuang Houzao San could not be fully controlled
At the same time, the inventor also referred to the thin-layer chromatography identification method of silkworm medicinal materials in the literature to detect Tianhuanghouzao powder, and found that the negative interference was serious and the spots could not be effectively separated.

Method used

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  • Quality detection method for tabasheer and monkey bezoar powder
  • Quality detection method for tabasheer and monkey bezoar powder
  • Quality detection method for tabasheer and monkey bezoar powder

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0044] 1. Preparation of the test solution:

[0045] Take 1-2 g of the test product (i.e. Tianhuanghouzao powder), add a certain amount of alcohol solution as the extraction solvent, ultrasonicate for more than 10 minutes, filter, evaporate the filtrate to dryness, add 1ml of methanol to the residue to dissolve it, and use it as the test sample. product solution.

[0046] 2. Preparation of the reference medicinal material solution:

[0047] Take Bombyx mori medicinal material powder and make reference medicinal material solution according to the preparation method of the test solution.

[0048] 3. Use thin-layer chromatography identification method to identify the test product:

[0049] TLC conditions are as follows:

[0050] Thin layer board: ordinary silicone G prefabricated board.

[0051] Spotting: Spot 1μl~5μl respectively.

[0052] The first developing agent: certain volume ratio of cyclohexane, ethyl acetate and formic acid.

[0053] The first expansion method: upwa...

Embodiment 1

[0064] A kind of embodiment of the quality detection method of Tianhuanghouzao powder of the present invention comprises the following steps:

[0065] 1. Preparation of test solution:

[0066] Take 1g of the test product powder, add methanol 20ml, ultrasonic for 20 minutes, filter, evaporate the filtrate to dryness, add methanol 1ml to dissolve the residue, and use it as the test solution.

[0067] 2. Preparation of the reference medicinal material solution:

[0068] Take 1 g of silkworm medicinal material powder, and make a control medicinal material solution according to the preparation method of the test solution.

[0069] 3. Use thin-layer chromatography identification method to identify the test product:

[0070] TLC conditions are as follows:

[0071] Thin layer board: ordinary silicone G prefabricated board.

[0072] Spotting: Spotting 4 μl of the test product solution and 1 μl of the control medicinal material solution.

[0073] The first developer: cyclohexane: e...

Embodiment 2

[0083] An embodiment of the quality detection method of Tianhuanghouzao powder of the present invention, the specific operation steps are the same as in Example 1, the difference is that the extraction solvent used in step 1 is ethanol, and the ultrasonic time is 10 minutes; in step 3, cyclohexane, The volume ratio of ethyl acetate and formic acid is 5:5:0.4, and the volume ratio of ethyl acetate, formic acid and water is 5:1.5:1.5.

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Abstract

The invention discloses a quality detection method for tabasheer and monkey bezoar powder. The method comprises the following steps: (1) performing primary developing on a tabasheer and monkey bezoarpowder test sample solution and a bombyx batryticatus reference crude herb solution on a thin layer plate, obtaining corresponding chromatograms through a chromatographic instrument, and comparing thechromatograms of the two solutions so as to judge whether the tabasheer and monkey bezoar powder contains a bombyx batryticatus medicinal material and eliminate negative interference; and (2) if so,performing secondary developing on the thin layer plate in the step (1), performing contrastive analysis on the chromatograms of the two solutions obtained by the secondary developing so as to determine whether the tabasheer and monkey bezoar powder contains the bombyx batryticatus. According to a thin-layer chromatography identification method, two different developing agent systems are adopted to detect different components in the bombyx batryticatus, the specificity of bombyx batryticatus detection is obviously improved, and negative interference caused by scorpio is eliminated.

Description

technical field [0001] The invention relates to the technical field of drug quality detection, in particular to a quality detection method for Tianhuanghouzao powder. Background technique [0002] Pediatric medicine refers to the medicine used for children's diseases. Compared with adult medicine, the safety of pediatric medicine is more important. For Chinese patent medicines, since the mechanism of action is not clear, the quality requirements need to be stricter to ensure the safety of medication. Tianhuang Houzao San is a commonly used Chinese patent medicine in pediatrics. Blindly composed of medicinal materials, the currently implemented standard is "Ministry of Health Drug Standards Traditional Chinese Medicine Prescriptions Volume 10", the standard number is WS 3 -B-1889-95. The preparation method of Tianhuang Houzao powder is as follows: the above eleven ingredients, monkey date, pearl, scorpion, silkworm, dannanxing, Tianzhuhuang, Gastrodia elata are respectivel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 陈晓坚江涛彭红英苏锐辉杨滨宾陈丽斯程君璐
Owner GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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