Primer composition for guiding nitroglycerin medication and healthy drinking, multiple gene detection kit and use method of kit
A technology of primer composition and nitroglycerin, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of high technical cost, affecting sensitivity, and difficult conditions to control, so as to achieve high detection sensitivity and improve detection Efficiency and the effect of saving testing costs
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Embodiment 1
[0040] The present invention is a multiple gene detection kit for guiding nitroglycerin medication and healthy drinking. When in use, the patient's blood or oral swab sample is collected to extract nucleic acid, and the patient's nucleic acid is used as a template to carry out PCR reaction, and finally the sample is separated by capillary electrophoresis. The specific steps as follows:
[0041] 1. Production of multiple gene detection kits to guide nitroglycerin medication and healthy drinking, the components included in the kit:
[0042] 1)PCR Primer (PCR Primer Mix):
[0043] 2) 25mM magnesium chloride (MgCl 2 )
[0044] 3) DNA polymerase (Taq DNA Polymerase)
[0045] 4) Solution X (Solution X)
[0046] 5) PCR buffer (PCR Buffer)
[0047] 6) Positive control (Positive Control)
[0048] 7) ultrapure water (ddH 2 O)
[0049] The above-mentioned PCR primers include the following 2 forward and reverse amplification primers of different genotypes on the 2 SNP sites on th...
Embodiment 2
[0074] Detection reagent specificity analysis: Single-plex PCR amplification is detected as a single peak of the target fragment size by capillary electrophoresis.
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