43results about How to "No false negative results" patented technology

The invention discloses a multi-gene detection kit for guiding administration of 5-fluorouracil and a detection method of the multi-gene detection kit. The multi-gene detection kit comprises ultrapure water, an X solution, a 10*PCR (Polymerase Chain Reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (Deoxyribonucleic Acid) polymerase and a positive control, and is characterized in that the PCR primer comprises six forward and reverse amplification primers with different genotypes on seven SNP (Single Nucleotide Polymorphism) sites of genes related to the administration of 5-fluorouracil, and forward and reverse amplification primers of a DNA internal reference and a reaction internal reference, and gene sequences of the forward and reverse amplification primers are shown as SEQ ID NO. 1-32 (Sequence Identifier Numbers 1-32). The detection method of the multi-gene detection kit comprises the steps of collecting a sample, extracting nucleic acid, conducting a PCR by taking the extracted nucleic acid as a template, and conducting capillary electrophoretic separation on the sample with a GeXP genetic analyser. The multi-gene detection kit and the detection method have the advantages of strong specificity, high accuracy, flux and reliability, low cost and no false negative result.

The invention discloses a kit for synchronously detecting twenty-two respiratory tract pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine respiratory tract pathogens, and a reverse primer of a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-6 (sequence identifier number 1-6), and the PCR primer comprises forward and reverse PCR amplification primers of the rest thirteen respiratory tract pathogens, a forward and reverse amplification primer of a human DNA internal reference, a forward and reverse PCR amplification primer of a reaction internal reference, PCR amplification primers of the nine respiratory tract pathogens, and a PCR amplification primer of the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 7-44. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention discloses a primer composition, a kit and a use method for detecting the expression level of insulin-like peptide ILP series genes in Drosophila melanogaster. The primer composition comprises RT amplification primers and PCR amplification primers of insulin-like peptide and RNA internal reference. The kit includes DEPC water, 5×RT buffer, reverse transcription primer, reverse transcriptase, Z solution, 10×PCR buffer, PCR primer, 25mM magnesium chloride solution, DNA polymerase and positive control. The present invention can detect 7 insulin-like peptides of Drosophila melanogaster at the same time, and can complete the detection of 192 samples in one day, which not only saves production and detection costs, but also improves detection efficiency; the RNA internal reference provides a control internal reference for the integrity of the sample RNA, Ensure the judgment of sample quality during the inspection process, avoid false negatives, and make the detection have better sensitivity and specificity, thereby avoiding the problem of low specificity of other detection methods.

The invention discloses a kit for synchronously detecting thirteen diarrhea viruses and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the eleven diarrhea RNA viruses and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-12 (sequence identifier number 1-12), and the PCR primer comprises forward and reverse PCR amplification primers of the rest two diarrhea viruses, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the eleven diarrhea RNA viruses and the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 13-32. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.