Kit for synchronously detecting related gene expression level of 14 antitumor drugs by using paraffin embedding biopsy sample, and detection method thereof
A gene expression level, paraffin-embedded technology, used in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc. and other problems, to achieve the effect of saving detection cost, superior low cost, and solving easy pollution
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specific Embodiment 2
[0070] The present invention is a method for synchronous detection of 14 anti-tumor drug-related genes using paraffin-embedded section samples, collecting patient tumor tissue to extract nucleic acid, performing reverse transcription and PCR reaction with patient nucleic acid as a template, and finally separating samples by capillary electrophoresis ,Specific steps are as follows:
[0071] 1. Production of a kit for synchronous detection of 14 anti-tumor drug-related genes based on the GeXP multiple gene expression genetic analysis system using paraffin-embedded section samples. The components included in the kit are the same as those in Example 1 above;
[0072] 2. Collect samples and extract nucleic acids
[0073] Collect isolated cultures of paraffin-embedded section samples of tumor tissue from patients, and extract nucleic acids from the isolated cultures;
[0074] 3. Reverse transcription (RT) reaction using patient nucleic acid as a template
[0075] 1) Add reagents ...
specific Embodiment 3
[0099] Detection Kit Sensitivity and Specificity Analysis
[0100] Sensitivity analysis: After diluting the positive control substance by a certain ng value, it is detected by PCR amplification and capillary electrophoresis until no signal is detected. The ng value is the lowest detection line, which is the sensitivity of the kit. The sensitivity of this kit is 1.0ng.
[0101] Specificity analysis: Single-plex PCR amplification is detected as a single peak of the target fragment size by capillary electrophoresis.
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