Multi-gene detection kit for guiding administration of platinum drugs and detection method of multi-gene detection kit

Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) detection kit for guiding administration of platinum drugs and a detection method of the detection kit. The detection kit comprises an X solution, a 10*PCR (Polymerase Chain Reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (Deoxyribonucleic Acid) polymerase and a positive control, and is characterized in that the PCR primer comprises six forward and reverse amplification primers with different genotypes on eight SNP sites of genes related to the administration of the platinum drugs, and forward and reverse amplification primers of a human DNA internal reference and a reaction internal reference, and gene sequences of the forward and reverse amplification primers are shown as SEQ ID NO. 1-36 (Sequence Identifier Numbers 1-36). The detection method of the detection kit comprises the steps of collecting a sample, extracting nucleic acid, conducting a PCR by taking the extracted nucleic acid as a template, and conducting capillary electrophoretic separation on the sample with a GeXP genetic analyser. The detection kit and the detection method have the advantages of strong specificity, high accuracy, flux and reliability, low cost and no false negative result.

Description

technical field [0001] The invention relates to a multiple gene detection kit and a detection method thereof, in particular to a multiple gene detection kit for guiding the administration of platinum drugs and a detection method thereof. Background technique [0002] Platinum drugs are the general term for biologically active platinum-containing complex drugs, and are currently one of the most commonly used tumor chemotherapy drugs in clinical practice, including cisplatin, carboplatin, and oxaliplatin. According to statistics, in my country's anticancer chemotherapy regimens, cisplatin-based or cisplatin-based regimens account for 70% to 80% of all chemotherapy regimens. However, in the clinical application of platinum-based drugs, there are problems of low treatment efficiency and large side effects, and there are significant individual differences. Some patients benefit, while some patients are drug-resistant and have severe side effects. [0003] The pharmacological mec...

AI Technical Summary

Problems solved by technology

Its disadvantages are (1) low throughput: it is not suitable for the detection of multiple SNP sites; it is difficult to set internal control genes

(2) High cost: the cost of probe labeling is high; if you need to obtain all relevant SNP information, you need to conduct multiple detection tests, and the superimposed cost is more expensive

Disadvantages: 1) The hybridization kinetics between different SNP sites are different, and the conditions are difficult to control when performing simultaneous detection of multiple sites; 2) The technology is expensive and complicated: each sample requires a chip, and the cost is more than ￥1000 / sample , which is not conducive to large-scale promotion; the synthesis and fixation of probes are relatively complicated, especially the production of high-density probe arrays, which is the main speed-limiting step; 3) poor repeatability, low accuracy, and false positive and false negative results are prone to occur; 4) Low sensitivity: the chip method requires a large amount of nucleic acid, and generally requires multiple PCR amplification first. Due to the large number of primers, it is easy to produce dimers and hairpin structures by itself, or the purpose of amplification is caused by different Tm values. Different fragment efficiencies will affect the detection sensitivity; 5) Due to the variety of chips, it is difficult to formulate a unified quality control standard

[0016] At present, there are no relevant research reports on multiple gene detection kits and detection methods based on the GeXP multiple gene expression genetic analysis system to guide platinum-based drug use at home and abroad.

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