Respiratory tract infection multiple detection reagent kit and detection method

Abstract

The invention provides a respiratory tract infection multiple detection reagent kit and detection method. Particularly, a multiplex respiratory tract pathogen PCR amplification system is designed and subjected to experimental verification, so that a multiplex fluorescent PCR amplification system for detecting various respiratory tract pathogens is obtained, and a single system can detect 3 kinds of respiratory tract pathogens at the same time. The reagent kit has high sensitivity and specificity. Through the reagent kit, quick detection and analysis of multiplex respiratory tract pathogens in samples of nasopharyngeal swabs, bronchoalveolar lavage fluid, phlegm and the like can be realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular, the invention relates to a multiple detection kit and detection method for respiratory tract infection. Background technique [0002] Acute respiratory tract infection (ARTI) is one of the most common infectious diseases in humans worldwide, and its morbidity ranks first among acute infectious diseases. On average, lower respiratory tract infections cause about 4,000,000 deaths each year. Globally, respiratory infections are the leading cause of death in children under the age of 5. ARTIs are generally caused by bacteria and viruses, of which about 90% are caused by respiratory viruses, but death and severe cases are mainly caused by bacterial infections. Faced with the current complex situation of prevention and control of respiratory infectious diseases, strengthening the monitoring of epidemic information of infectious diseases and strains can provide a basis for formulating pr...

AI Technical Summary

Problems solved by technology

However, this method is complex, cumbersome to operate, long cycle, low sensitivity, relatively high requirements for samples, and prone to false negative results

[0006] Serological testing is based on the principle of antigen-antibody reaction. The detection cycle is short, but the specificity and sensitivity are low, and false negative and false positive results are prone to occur, which has certain limitations.

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