The invention discloses a dietary supplement and a preparation method thereof. The dietary supplement comprises two varieties of plant extractives, nine varieties of vitamins and two varieties of minerals. Active components comprise by weight 12-18 parts of rhodioloside, 100-114 parts of proanthocyanidins, 0.7-0.9 part of vitamin A, 1-3 parts of vitamin B1, 1-3 parts of vitamin B2, 3-5 parts of vitamin 6, 0.004-0.006 part of vitamin B12, 80-150 parts of vitamin C, 40-60 parts of vitamin E, 0.3-0.5 part of folic acid, 12-18 parts of nicotinamide, 5-15 parts of zinc and 0.02-0.06 part of selenium. The dietary supplement of the invention has comprehensive nutrition, can enhance antioxidation capability and reduce electromagnetic radiation damage, and is suitable for daily dietary supplement of radar troops as well as population living and working for long time in environment of high electromagnetic radiation, low illumination and continuous noise.