76 results about "Rhodioloside" patented technology

The invention discloses a method for extracting salidroside from rhodiola. The method for extracting the salidroside from the rhodiola comprises the following steps of (1) smashing a rhodiola medicinal material, using water as solvent, carrying out ultrasonic-assisted extraction for 1-4 times, combining extracting solutions, and filtering the combined solutions to obtain filtrate A; (2) enabling the filtrate A to pass through a macroporous resin column, collecting eluant B of the salidroside, and carrying out vacuum concentration on the eluant B to obtain a dried object C; (3) dissolving the dried object C with absolute ethyl alcohol, carrying out separation on the dried object C through simulated moving bed chromatography, and obtaining a constituent D rich in the salidroside; (4) carrying out concentration, crystallization, centrifugation and drying to obtain a salidroside product. The method for extracting the salidroside from the rhodiola is easy and convenient to operate, broad in application prospect in industries, and high in yield and product purity; according to the technical scheme, the yield of the obtained salidroside reaches more than 70%, and the content of the salidroside contained in the obtained product is more than 98%.

The invention discloses a production method for separation and purification of salidroside from rhodiola rosea. The method comprises the following steps: after being smashed, raw materials are thrown in from a throwing hopper; a mainframe of an extraction tank set rotates; the materials are slowly propelled from the front end of the set to the back end; and at the same time, extraction solvent is introduced in the extraction tank from a liquid inlet pipe at the tail end of the set, and penetrates the movable materials from the back end of the tank to flow to the front end; and the solid-phase and liquid-phase materials are fully contacted with each other in the inverse movement so as to extract the effective components of medicinal materials. Medicine dregs are forcedly pushed to a dreg outlet through a discharge conveyer for discharging, and are extruded by using a special juice extruder to extrude residual medicine juice from medicinal material organizations, so that the content of the residual medicine juice of the medicine dregs is reduced, the extraction efficiency is higher, and the extraction is more thorough. The method chooses macroreticular resins and combines with the silica column chromatography to purify, so that better separation effect is achieved. Finished products of salidroside with the highest yield of 1% and the content above 95% can be obtained.

The invention relates to a method for extracting salidroside from rhodiola rosea. The method comprises the following treatment steps: by taking natural rhodiola rosea as a raw material, drying, grinding, allowing the rhodiola rosea to pass through a sieve with the granularity of 80 meshes, thereby obtaining rhodiola rosea powder; performing bio-enzyme enzymolysis; performing microwave assisted extraction; purifying through a macroporous resin; and crystallizing. According to the method disclosed by the invention, the rhodiola rosea is taken as the raw material, bio-enzyme enzymolysis is combined with microwave extraction, and cell walls of plants are destroyed, so that active ingredients in the cells are fully dissolved out, the extraction rate of the active ingredients is increased, and the method has the advantages of mild reaction conditions, high speed, low energy consumption, high quality, time conservation, no pollution and the like. Moreover, the microwave assisted extraction mode has the advantage that the content of protein impurities in the extracting solution is low and is suitable for industrial production.

The invention discloses an extract of a tyrosol plant, which is extracted from a root or a rhizome of a rhodiola rosea or from a mature fruit or a flower of a glossy privet fruit or from fermented soy sauce or vinegar residue. The invention also discloses an extract preparation of the tyrosol plant and the purpose of the extract preparation. The invention also discloses an extract of a salidroside plant of the tyrosol side product and the preparation and purpose of the extract. The extraction technology of the extracts of the invention is advanced, and the content of the effective components of the extracts is high, and the extracts can be fit for the requirements of the industrialized production technology and the pharmacy.

The invention discloses a making method of rhodiola root glycoside,which comprises the following steps: a. adding water to dissolve crude extract of rhodiola root; adjusting pH value as alkaline; filtering to obtain the filtrate; b. adjusting the filtrate to acid; adsorbing through adsorbing resin; eluting; condensing the eluent; sedimenting the eluent in the alcohol; filtering to obtain the filtrate; c. drying the filtrate through adsorbing resin; adding the dried material into column chromatograph; collecting the chromatographic liquor; d. condensing the chromatographic liquor; doing alcohol sediment; crystallizing. The invention also provides freeze dried and injection of the rhodiola root glycoside with simply and controllably operating condition and high safety, which is easy to accomplish for large scale of industrial manufacturing.

The invention relates to a rhodiola rosea and a rhodioside application for inducing stem cells to directionally differentiate into hepatocytes, in particular to a compound inducer of the rhodioside and cell growth factor FGF-4 used for inducing mesenchymal stem cells to directionally differentiate into hepatocytes. The rhodiola rosea develops the new application of rhodiola, which induces mesenchymal stem cells and other stem cells to directionally differentiate into hepatocytes in vitro, and provides the effect of the rhodiola rosea and the rhodioside on inducing the adult stem cells to directionally differentiate into hepatocytes, which is beneficial to further research of the in vivo stem cells transplantation for treating acute or chronic hepatic injury and middle-advanced liver disease field, and provides the basic for new drug development.

The invention discloses a dietary supplement and a preparation method thereof. The dietary supplement comprises two varieties of plant extractives, nine varieties of vitamins and two varieties of minerals. Active components comprise by weight 12-18 parts of rhodioloside, 100-114 parts of proanthocyanidins, 0.7-0.9 part of vitamin A, 1-3 parts of vitamin B1, 1-3 parts of vitamin B2, 3-5 parts of vitamin 6, 0.004-0.006 part of vitamin B12, 80-150 parts of vitamin C, 40-60 parts of vitamin E, 0.3-0.5 part of folic acid, 12-18 parts of nicotinamide, 5-15 parts of zinc and 0.02-0.06 part of selenium. The dietary supplement of the invention has comprehensive nutrition, can enhance antioxidation capability and reduce electromagnetic radiation damage, and is suitable for daily dietary supplement of radar troops as well as population living and working for long time in environment of high electromagnetic radiation, low illumination and continuous noise.

The invention provides a method for simultaneously preparing gram-grade high-purity tyrosol, renulatin and salidroside from rhodiola crenulata. With rhodiola crenulata as a raw material, the gram-grade high-purity tyrosol, renulatin and salidroside from rhodiola crenulata are prepared from such steps as ethanol extraction, alcohol precipitation, filtration, macroporous adsorption resin separation and silica gel column purification. The method is simple, convenient and quick, and provides a new way of thinking for the mass preparation of the three compounds.

The invention provides an induction composition of human mesenchymal stem cells, an induction differentiation culture liquid and an in-vitro induction method and an application, and relates to the field of nerve cell regeneration. The induction composition includes a brain-derived neurotrophic factor, a nerve growth factor, an epidermal cell growth factor, a fibroblast growth factor, a N2 cell culture additive, a B27 cell culture additive, rhodioloside and astragalus polysaccharide. In the induction composition provided by the invention, rhodioloside, astragalus polysaccharide and cytokines are compounded, induced differentiation nerve cells have the advantages of high content, strong activity, short period and low cost, can solve the problem of low differentiation efficiency when human mesenchymal stem cells are induced in vitro, improve the survival status of the induced nerve cells, and have little toxic and side effects on cells. The induction composition can be used for inducing the human mesenchymal stem cells, is easy to operate, enhances the activity of the differentiated nerve cells and shortens the induction differentiation time.

The invention belongs to the technical field of medicaments or foods and discloses a medicinal composition. The medicinal composition comprises the following raw materials in part by weight: 10 to 1,000 parts of rhodioloside and 1 to 200 parts of D-ribose. The medicinal composition of the invention has a therapeutical effect on myocardial ischemia and anoxia, also can improve cardiovascular functions, controls the accumulation of blood platelets and the formation of thrombus, and has a certain effect of reducing blood lipid. The medicinal composition of the invention has the effects of tonifying qi, invigorating blood circulation, promoting coronary circulation and relieving pain, is used for chest obstruction which is caused by qi deficiency and blood stasis and is manifested as chest stuffiness, stabbing pain or dull pain, palpitation, short breath, fatigue, acratia, lack of qi and no desire to speak, light-headedness and the like, and is used for coronary heart disease and angina pectoris with above expressions.

The present invention relates to effective Chinese medicine component extracting technology, and is especially supercritical CO2 fluid extraction process for extracting rhodiola glycoside as the effective component of Rhodiola rosea. The present invention adopts orthogonal experiment design to screen out technological conditions for the supercritical CO2 fluid extraction process. In the conditions of Rhodiola rosea material granularity lower than 0.25 micron, extracting temperature of deg.c, CO2 fluid pressure of 300 Par, entrainer ethanol flow rate of 0.4 ml / min and extracting time of 2 hr, rhodiola glycoside extracting rate may reach 1.398 wt%, 3.56 times higher than traditional reflux process. Compared with traditional process, the present invention has the features of high speed, high accuracy, high efficiency and high economical efficiency.

The invention provides an application of rhodiola rosea and salidroside in inducing directional differentiation of stem cells into cardiomyocyte-like cells, especially salidroside and low oxygen combined as an inducer for inducing directional differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells. The invention provides a new use of salidroside, namely a use in inducing directional differentiation of the stem cells such as adipose-derived stem cells into the cardiomyocyte-like cells in vitro; provided is the effect of rhodiola rosea and salidroside in inducingdirectional differentiation of adult stem cells into cardiomyocyte-like cells; in-depth study of in vivo stem cell transplantation treatment of acute and chronic myocardial injury and cardiovascular disease fields is facilitated, and a basis is provided for the development of new drugs.

The invention discloses an extraction method of salidroside. The extraction method comprises the following steps: A, picking fresh rhodiola rosea, removing the fresh rhodiola rosea with diseases and pests, and drying; B, grinding the dry rhodiola rosea into powder, soaking the ground rhodiola rosea, performing percolation to obtain a percolation solution; C, collecting supernatant after the percolation solution stands, and removing polysaccharides and proteins by virtue of the adsorption of resin; after a sample is loaded, flushing by using pure water until the sample is colorless, then performing gradient elution by using an elution solution of 4 to 8 percent of ethanol aqueous solution, 14 to 18 percent of ethanol aqueous solution, 24 to 28 percent of ethanol aqueous solution, 34 to 38percent of ethanol aqueous solution and 44 to 48 percent of ethanol aqueous solution, wherein the pH value of the elution solution is 6.4 to 6.6, separately collecting each gradient elution solution,concentrating to obtain five groups of salidroside crude products; and D, separating impurities and purifying each group of salidroside crude product to obtain high-purity salidroside. By adopting themethod, the salidroside is extracted, the extraction rate is high, and the purity of the extracted salidroside is high.

The invention discloses herba rhodiolae extract. According to the herba rhodiolae extract, the content of rhodioloside is 2.0 percent or more and the total extraction yield is 28 percent or more. A preparation method of the herba rhodiolae extract comprises the following steps: (1) pre-treating raw materials: taking dried herba rhodiolae and crushing; carrying out high-pressure instant blasting pre-treatment; (2) extracting: extracting the herba rhodiolae with 70 percent to 80 percent of an ethanol solution; (3) eluting: adsorbing and purifying with macroporous adsorption resin and eluting, combining an eluting solution; (4) concentrating: concentrating, boiling and granulating or carrying out spray-drying to obtain the herba rhodiolae extract. The invention further discloses a healthy product containing the herba rhodiolae extract; the healthy product has the effects of adjusting and protecting liver, lowering blood pressure and blood viscosity, improving the immunity and the like and can be used as a health-care food for eating; the healthy product is prepared from the following raw materials: 1 part of the herba rhodiolae extract, 1 to 5 parts of radix puerariae extract, 0.8 to 1.2 parts of American ginseng extract, 0.8 to 2.5 parts of ganodorma lucidum extract and 0.8 to 1.0 part of chitosan oligosaccharide.

The invention discloses a traditional Chinese medicine effective part composition capable of treating liver cancer. The composition contains total alkaloids from solanum nigrum extracted from the traditional Chinese medicine Solanum nigrum and salidroside extracted from perennial herb rhodiola root, Rhodiola, Crassulaceae; and the medicine composition has remarkable treatment effect on the liver cancer and has the efficacy of protecting livers. In the effective part composition, the content of the total alkaloids from solanum nigrum of a Solanum nigrum extract is more than 50 percent, and the content of the salidroside in a Rhodiola rosea extract is more than 50 percent. In the medicine composition, the content of the total alkaloids from solanum nigrum is 2 to 6 weight portions more than 50 percent of the Solanum nigrum extract, and the content of the salidroside is 1 to 3 weight portions more than 50 percent of the Rhodiola rosea extract, wherein the preferred proportion is that the content of the total alkaloids from solanum nigrum is 2 weight portions more than 50 percent of the Solanum nigrum extract and the content of the salidroside is 1 weight portion more than 50 percent of the Rhodiola rosea extract. The invention also discloses a method for preparing the medicine composition.

The invention discloses a semi-bionic extraction process for extracting rhodioloside from rhodiola crenulata. On one hand, an extracting process of a semi-bionic extraction method accords with traditional Chinese medicine compatibility and clinical medicine characteristics and characteristics of translocation and absorption of oral medicines in gastrointestinal tracts. On the other hand, the loss of effective components is less, and the rhodioloside in the rhodiola crenulata can be extracted to the greatest extent. The effective components in the rhodiola crenulata are dissolved into an extracting solution and the extracting solution is stewed by adopting ethyl ether; the rhodioloside is not dissolved into the ethyl ether and impurity removal treatment can be carried out on the extracting solution, so that the purity of the extracting solution is improved. Moderately polar macroporous resin is used for eluting the extracting solution and then the extracting solution is concentrated, centrifuged and dried; finally, the high-purity rhodioloside is extracted to the greatest extent. A rhodioloside finished product with the yield of 1.6 percent to 2.1 percent and the content of 97 percent or above can be obtained.

The invention discloses a preparation method of rhodioloside and a kit. The preparation method comprises following steps: mixing recombinant heatproof sucrose phosphorylase, recombinant heatproof cellobiose phosphorylase, cane sugar, tyrosol, and phosphoric acid to obtain a reaction mixture; then converting the reaction mixture at a temperature of 40 to 55 DEG C to generate rhodioloside. The preparation method has the advantages of simple technology, low cost, and high yield.

The invention relates to a novel application of an effective component rhodioloside of a plant rhodiola rosea. Integral animal experiment researches indicate that rhodioloside has a certain protective effect on experimental depression, and the action mechanism of the rhodioloside is possibly related to inflammation reaction inhibition (the levels of inflammatory factors IL-6 (interleukin-6) and TNF-alpha (tumor necrosis factor-alpha) in serum can be reduced), change of monoaminergic reaction in the brain (the content of neurotransmitter serotonin and noradrenaline in the brain can be increased), increase of expression of brain-derived neurotrophic factor (BDNF) in the brain, and the like. Therefore, the rhodioloside has an application prospect of development of anti-depression medicines.

The invention discloses a Chinese medicinal composition for resisting anoxia and fatigue and a preparation method thereof. The Chinese medicinal composition is prepared by adding excipients such as a disintegrant, a flavoring agent and the like into rhodiola rosea and borneol which are taken as raw materials. The content of rosavin and salidroside contained in a rhodiola rosea extract obtained by the method is improved; and the process is simple. Pharmacodynamic experiment results of the Chinese medicinal composition show that the medicine has remarkable effects of resisting anoxia and fatigue. The Chinese medicinal composition and the preparation method have the advantages that: the formula is simple, the preparation process is simple, and the medicine is convenient to use and has no toxic or side effect.

The invention relates to a low temperature and atmospheric pressure plasma extraction technology of rhodioloside, and belongs to the technical field of extraction of effective components in traditional Chinese medicines. Boiling extraction is adopted and assisted with low temperature and atmospheric pressure plasma extraction, coarse separation of macroporous adsorption resin and silicone rectification, so that the problem that the high-purity rhodioloside cannot be efficiently extracted by the prior art is solved. By adopting control parameters disclosed by the invention, the low temperature and atmospheric pressure plasma treatment technology can be led to traditional Chinese medicine extraction, so that the high-purity rhodioloside can be efficiently extracted.

The invention discloses a method using Rhodiola rosea liquid-submerged fermentation to produce rhodioloside. A liquid fermentation culture medium used by the method is prepared by, by weight percentage, 6-10% of glucose, 2-6% of Rhodiola rosea powder, 0.04-0.15% of MgSO4.3H2O, 0.04-0.15% of CaCl2, 0.2-0.5% of K2HPO4.3H2O, 0.2-0.45% of KH2PO4 and the balance water. The method has the advantages that the yield of the rhodioloside is increased evidently, activated carbon is used to decolor fermentation liquor, the membrane separation technology and the sephadex gel filtration chromatography technology are combined to separate and purify the fermentation liquor, high yield is achieved, and the obtained rhodioloside is high in purity and yield.

The invention belongs to the technical field of medicine, and relates to a technique for extracting and separating salidroside from glossy privet fruit, which comprises the following steps: (1) pulverizing glossy privet fruit as a raw material, and extracting the pulverized glossy privet fruit with water or ethanol as an extraction solvent, thereby obtaining an extracting solution; (2) concentrating the extracting solution in the step (1), and hydrolyzing by adding glacial acetic acid into the concentrated solution; and (3) preparing the hydrolysate in the step (2) into the crude salidroside product. In the invention, the salidroside is extracted and separated from the plant other than Rhodiola rosea, thereby relieving the pressure of inadequate resources; the acid hydrolysis method is utilized to enhance the content of salidroside in the glossy privet fruit; and the operational method is simple, the later processing is easy, the raw material for hydrolysis is nontoxic, the filler is cheap, and the solvent part can be recycled and reutilized, thereby saving the cost.

The invention discloses a method for efficiently extracting salidroside and rosavin from rhodiola rosea. The method comprises the following steps of: selecting rhodiola rosea roots, drying, and rapidly air-cooling; crushing the dried rhodiola rosea roots, and screening into the granularity of 40 meshes; adding 2% of hexadecyl trimethyl quaternary ammonium bromide and 80% alcohol solution of 1% of potassium acetate in an amount of 10 times of the weight of the rhodiola rosea into the obtained rhodiola rosea powder, performing dynamic countercurrent extraction at a temperature of 25 DEG C so as to obtain an extracting solution; performing vacuum concentration, concentrating into an extract proportion of 1.15-1.20; adding acidified pure water in an amount of 7 times of the weight into the obtained extract, fully stirring, precipitating in a refrigerated storage and staying overnight, and separating the precipitate; and performing spray drying, thereby obtaining the product. The production method provided by the invention is a one-step extraction method, the dynamic countercurrent extraction is adopted, extraction, separation and purification are simultaneously performed, the method is high in extraction speed, high in efficiency, low in energy consumption, simple in process and low in cost, and the product recovery rate is obviously improved.

The present invention relates to a method for manufacturing a rhodiola fermentation product by using an adsorbent. The method for manufacturing the rhodiola fermentation product of the present invention includes: for producing the rhodiola fermentation product, processing a rhodiola extract by the adsorbent of at least one selected from the group consisting of emathlite, bentonite, diatomaceous earth, activated carbon, and weakly basic anion exchange resin to get a filtrate, and inoculating lactic acid bacteria for fermentation. The rhodiola fermentation product manufactured by the method of the invention is high in the content of salidroside aglycone, and thus can be used as an active ingredient in novel health function foods or medicines.

The invention discloses a method for synthesizing rhodioloside based on microwave assistance and application of the synthesized rhodioloside to an anti-ageing preparation and belongs to the field of drug synthesis. The method comprises the following steps: putting pentaacetylated glucose, p-hydroxyethyl benzaldehyde and dichloromethane into a container; adding a 4A molecular sieve to obtain a mixed raw material solution; storing the mixed raw material solution under the temperature condition of 10 DEG C and slowly dropwise adding a dichloromethane solution of anhydrous stannic tetrachloride; after reacting for 3h, adding a reducing agent; after sufficiently reacting, adding an acetic acid solution and a catalyst in sequence; after reacting or 50 to 60min, adding a mixed solution prepared from sodium methoxide and methanol according to the volume ratio of 2 to 1; after reacting, adding a decoloring agent and carrying out decoloring treatment for 10 to 30min; heating by microwaves and controlling the temperature to be 70 to 75 DEG C; extracting and purifying a rhodioloside crude product solution to obtain a rhodioloside fine product. The synthesis method provided by the invention hasthe advantage of high yield.

The invention provides an oral liquid for treating gastric mucosa and liver injury and a preparation method of the oral liquid. The oral liquid for treating gastric mucosa and liver injury is characterized by being prepared from the following raw materials in parts by weight: 120 parts by weight of astragalus membranaceus, 120 parts by weight of codonopsis pilosula, 120 parts by weight of root of kudzu vine, 80 parts by weight of rhodiola rosea, 50 parts by weight of pseudo-ginseng and 50 parts by weight of sea-buckthorn. According to the oral liquid and the preparation method thereof disclosed by the invention, a three-factor three-level L9 (34) orthogonal test is carried out by taking water addition, decocting duration and decocting times as investigation factors, and variance analysis and confirmatory experiment are implemented by the six active ingredients for treating the disease as designing weight coefficients, so that the optimum decocting conditions are optimized. Through the preparation method, the transfer rates of the six active ingredients in the formula for treating the disease, namely astragaloside, campanulin, puerarin, rhodioloside, isorhamnetin and ginsenoside Re, are improved to be equal to or more than 90%, and significantly higher than the transfer rates through various existing extracting methods at home; and pharmaceutical effect is improved and cost is reduced.

The invention discloses application of an aqueous extract of saffron total glycoside, rhodioside, astragalus polyose, matrine, pachman, vitamin C, vitamin E, aspirin and taxus chinensis to preparation of a medicament for preventing and treating the renal disease of a newborn Tibetan mastiff in a low-altitude region.

The invention discloses a composition having health care effects on cardiovascular and cerebrovascular anoxia and on toxicity of excess oxidation to a human body and a preparation method for the composition. The composition comprises a ginkgo biloba extract, a rhodiola root extract, a kudzu root extract, a grape seed extract and a wolfberry fruit extract, wherein the mass content of general flavone in the composition is 10 to 20%, and the mass content of rhodioloside is 1 to 5%. The preparation method for the composition comprises a step of extraction of the extracts from natural plant raw materials and a second step of uniformly mixing the extracts, wherein the extracts can be made into a capsule or tablet. The capsule or tablet of the composition has the advantages of no toxicity and side effects, a good curative effect and health care effects on cardiovascular and cerebrovascular anoxia and on toxicity of excess oxidation to the human body.

The invention relates to a method for preparing traditional Chinese medicine preparations, and particularly relates to a hyperthermic devitalization extraction method of a rhodiola rosea injection. Sofren injection has the effect of promoting blood circulation to remove stasis. According to the method, degrading enzyme in the rhodiola rosea medicinal material is inactivated in a short time in 80-90 DEG C high-temperature water by adopting an extracting technology of feeding materials in 80-90 DEG C high-temperature water, and salidroside is prevented from being decomposed by degrading enzyme in an aqueous solution in a process of heating cold water, and the product yield is improved.

The invention provides a method for producing rhodioloside by enzymatic conversion and fermentation with apple beta-glucosidase as a novel catalyst. The apple beta-glucosidase is prepared with high-efficiency by a gene recombination technique; 50 mM-1000 mM of tyrosol and 10 mM-500 mM of glucose are added and subjected to reaction for 20-50 h at the temperature of 37-50 DEG C, and the rhodiolosideis obtained.