75 results about "Rhodioloside" patented technology

The invention relates to a method for extracting salidroside from rhodiola rosea. The method comprises the following treatment steps: by taking natural rhodiola rosea as a raw material, drying, grinding, allowing the rhodiola rosea to pass through a sieve with the granularity of 80 meshes, thereby obtaining rhodiola rosea powder; performing bio-enzyme enzymolysis; performing microwave assisted extraction; purifying through a macroporous resin; and crystallizing. According to the method disclosed by the invention, the rhodiola rosea is taken as the raw material, bio-enzyme enzymolysis is combined with microwave extraction, and cell walls of plants are destroyed, so that active ingredients in the cells are fully dissolved out, the extraction rate of the active ingredients is increased, and the method has the advantages of mild reaction conditions, high speed, low energy consumption, high quality, time conservation, no pollution and the like. Moreover, the microwave assisted extraction mode has the advantage that the content of protein impurities in the extracting solution is low and is suitable for industrial production.

The invention discloses an extract of a tyrosol plant, which is extracted from a root or a rhizome of a rhodiola rosea or from a mature fruit or a flower of a glossy privet fruit or from fermented soy sauce or vinegar residue. The invention also discloses an extract preparation of the tyrosol plant and the purpose of the extract preparation. The invention also discloses an extract of a salidroside plant of the tyrosol side product and the preparation and purpose of the extract. The extraction technology of the extracts of the invention is advanced, and the content of the effective components of the extracts is high, and the extracts can be fit for the requirements of the industrialized production technology and the pharmacy.

The invention discloses a making method of rhodiola root glycoside,which comprises the following steps: a. adding water to dissolve crude extract of rhodiola root; adjusting pH value as alkaline; filtering to obtain the filtrate; b. adjusting the filtrate to acid; adsorbing through adsorbing resin; eluting; condensing the eluent; sedimenting the eluent in the alcohol; filtering to obtain the filtrate; c. drying the filtrate through adsorbing resin; adding the dried material into column chromatograph; collecting the chromatographic liquor; d. condensing the chromatographic liquor; doing alcohol sediment; crystallizing. The invention also provides freeze dried and injection of the rhodiola root glycoside with simply and controllably operating condition and high safety, which is easy to accomplish for large scale of industrial manufacturing.

The invention relates to a rhodiola rosea and a rhodioside application for inducing stem cells to directionally differentiate into hepatocytes, in particular to a compound inducer of the rhodioside and cell growth factor FGF-4 used for inducing mesenchymal stem cells to directionally differentiate into hepatocytes. The rhodiola rosea develops the new application of rhodiola, which induces mesenchymal stem cells and other stem cells to directionally differentiate into hepatocytes in vitro, and provides the effect of the rhodiola rosea and the rhodioside on inducing the adult stem cells to directionally differentiate into hepatocytes, which is beneficial to further research of the in vivo stem cells transplantation for treating acute or chronic hepatic injury and middle-advanced liver disease field, and provides the basic for new drug development.

The invention provides a method for simultaneously preparing gram-grade high-purity tyrosol, renulatin and salidroside from rhodiola crenulata. With rhodiola crenulata as a raw material, the gram-grade high-purity tyrosol, renulatin and salidroside from rhodiola crenulata are prepared from such steps as ethanol extraction, alcohol precipitation, filtration, macroporous adsorption resin separation and silica gel column purification. The method is simple, convenient and quick, and provides a new way of thinking for the mass preparation of the three compounds.

The invention discloses herba rhodiolae extract. According to the herba rhodiolae extract, the content of rhodioloside is 2.0 percent or more and the total extraction yield is 28 percent or more. A preparation method of the herba rhodiolae extract comprises the following steps: (1) pre-treating raw materials: taking dried herba rhodiolae and crushing; carrying out high-pressure instant blasting pre-treatment; (2) extracting: extracting the herba rhodiolae with 70 percent to 80 percent of an ethanol solution; (3) eluting: adsorbing and purifying with macroporous adsorption resin and eluting, combining an eluting solution; (4) concentrating: concentrating, boiling and granulating or carrying out spray-drying to obtain the herba rhodiolae extract. The invention further discloses a healthy product containing the herba rhodiolae extract; the healthy product has the effects of adjusting and protecting liver, lowering blood pressure and blood viscosity, improving the immunity and the like and can be used as a health-care food for eating; the healthy product is prepared from the following raw materials: 1 part of the herba rhodiolae extract, 1 to 5 parts of radix puerariae extract, 0.8 to 1.2 parts of American ginseng extract, 0.8 to 2.5 parts of ganodorma lucidum extract and 0.8 to 1.0 part of chitosan oligosaccharide.

The invention discloses a traditional Chinese medicine effective part composition capable of treating liver cancer. The composition contains total alkaloids from solanum nigrum extracted from the traditional Chinese medicine Solanum nigrum and salidroside extracted from perennial herb rhodiola root, Rhodiola, Crassulaceae; and the medicine composition has remarkable treatment effect on the liver cancer and has the efficacy of protecting livers. In the effective part composition, the content of the total alkaloids from solanum nigrum of a Solanum nigrum extract is more than 50 percent, and the content of the salidroside in a Rhodiola rosea extract is more than 50 percent. In the medicine composition, the content of the total alkaloids from solanum nigrum is 2 to 6 weight portions more than 50 percent of the Solanum nigrum extract, and the content of the salidroside is 1 to 3 weight portions more than 50 percent of the Rhodiola rosea extract, wherein the preferred proportion is that the content of the total alkaloids from solanum nigrum is 2 weight portions more than 50 percent of the Solanum nigrum extract and the content of the salidroside is 1 weight portion more than 50 percent of the Rhodiola rosea extract. The invention also discloses a method for preparing the medicine composition.

The invention discloses a semi-bionic extraction process for extracting rhodioloside from rhodiola crenulata. On one hand, an extracting process of a semi-bionic extraction method accords with traditional Chinese medicine compatibility and clinical medicine characteristics and characteristics of translocation and absorption of oral medicines in gastrointestinal tracts. On the other hand, the loss of effective components is less, and the rhodioloside in the rhodiola crenulata can be extracted to the greatest extent. The effective components in the rhodiola crenulata are dissolved into an extracting solution and the extracting solution is stewed by adopting ethyl ether; the rhodioloside is not dissolved into the ethyl ether and impurity removal treatment can be carried out on the extracting solution, so that the purity of the extracting solution is improved. Moderately polar macroporous resin is used for eluting the extracting solution and then the extracting solution is concentrated, centrifuged and dried; finally, the high-purity rhodioloside is extracted to the greatest extent. A rhodioloside finished product with the yield of 1.6 percent to 2.1 percent and the content of 97 percent or above can be obtained.

The invention discloses a preparation method of rhodioloside and a kit. The preparation method comprises following steps: mixing recombinant heatproof sucrose phosphorylase, recombinant heatproof cellobiose phosphorylase, cane sugar, tyrosol, and phosphoric acid to obtain a reaction mixture; then converting the reaction mixture at a temperature of 40 to 55 DEG C to generate rhodioloside. The preparation method has the advantages of simple technology, low cost, and high yield.

The invention relates to a novel application of an effective component rhodioloside of a plant rhodiola rosea. Integral animal experiment researches indicate that rhodioloside has a certain protective effect on experimental depression, and the action mechanism of the rhodioloside is possibly related to inflammation reaction inhibition (the levels of inflammatory factors IL-6 (interleukin-6) and TNF-alpha (tumor necrosis factor-alpha) in serum can be reduced), change of monoaminergic reaction in the brain (the content of neurotransmitter serotonin and noradrenaline in the brain can be increased), increase of expression of brain-derived neurotrophic factor (BDNF) in the brain, and the like. Therefore, the rhodioloside has an application prospect of development of anti-depression medicines.

The invention discloses a method using Rhodiola rosea liquid-submerged fermentation to produce rhodioloside. A liquid fermentation culture medium used by the method is prepared by, by weight percentage, 6-10% of glucose, 2-6% of Rhodiola rosea powder, 0.04-0.15% of MgSO4.3H2O, 0.04-0.15% of CaCl2, 0.2-0.5% of K2HPO4.3H2O, 0.2-0.45% of KH2PO4 and the balance water. The method has the advantages that the yield of the rhodioloside is increased evidently, activated carbon is used to decolor fermentation liquor, the membrane separation technology and the sephadex gel filtration chromatography technology are combined to separate and purify the fermentation liquor, high yield is achieved, and the obtained rhodioloside is high in purity and yield.

The invention belongs to the technical field of medicine, and relates to a technique for extracting and separating salidroside from glossy privet fruit, which comprises the following steps: (1) pulverizing glossy privet fruit as a raw material, and extracting the pulverized glossy privet fruit with water or ethanol as an extraction solvent, thereby obtaining an extracting solution; (2) concentrating the extracting solution in the step (1), and hydrolyzing by adding glacial acetic acid into the concentrated solution; and (3) preparing the hydrolysate in the step (2) into the crude salidroside product. In the invention, the salidroside is extracted and separated from the plant other than Rhodiola rosea, thereby relieving the pressure of inadequate resources; the acid hydrolysis method is utilized to enhance the content of salidroside in the glossy privet fruit; and the operational method is simple, the later processing is easy, the raw material for hydrolysis is nontoxic, the filler is cheap, and the solvent part can be recycled and reutilized, thereby saving the cost.

The present invention relates to a method for manufacturing a rhodiola fermentation product by using an adsorbent. The method for manufacturing the rhodiola fermentation product of the present invention includes: for producing the rhodiola fermentation product, processing a rhodiola extract by the adsorbent of at least one selected from the group consisting of emathlite, bentonite, diatomaceous earth, activated carbon, and weakly basic anion exchange resin to get a filtrate, and inoculating lactic acid bacteria for fermentation. The rhodiola fermentation product manufactured by the method of the invention is high in the content of salidroside aglycone, and thus can be used as an active ingredient in novel health function foods or medicines.

The invention discloses a method for synthesizing rhodioloside based on microwave assistance and application of the synthesized rhodioloside to an anti-ageing preparation and belongs to the field of drug synthesis. The method comprises the following steps: putting pentaacetylated glucose, p-hydroxyethyl benzaldehyde and dichloromethane into a container; adding a 4A molecular sieve to obtain a mixed raw material solution; storing the mixed raw material solution under the temperature condition of 10 DEG C and slowly dropwise adding a dichloromethane solution of anhydrous stannic tetrachloride; after reacting for 3h, adding a reducing agent; after sufficiently reacting, adding an acetic acid solution and a catalyst in sequence; after reacting or 50 to 60min, adding a mixed solution prepared from sodium methoxide and methanol according to the volume ratio of 2 to 1; after reacting, adding a decoloring agent and carrying out decoloring treatment for 10 to 30min; heating by microwaves and controlling the temperature to be 70 to 75 DEG C; extracting and purifying a rhodioloside crude product solution to obtain a rhodioloside fine product. The synthesis method provided by the invention hasthe advantage of high yield.

The invention provides an oral liquid for treating gastric mucosa and liver injury and a preparation method of the oral liquid. The oral liquid for treating gastric mucosa and liver injury is characterized by being prepared from the following raw materials in parts by weight: 120 parts by weight of astragalus membranaceus, 120 parts by weight of codonopsis pilosula, 120 parts by weight of root of kudzu vine, 80 parts by weight of rhodiola rosea, 50 parts by weight of pseudo-ginseng and 50 parts by weight of sea-buckthorn. According to the oral liquid and the preparation method thereof disclosed by the invention, a three-factor three-level L9 (34) orthogonal test is carried out by taking water addition, decocting duration and decocting times as investigation factors, and variance analysis and confirmatory experiment are implemented by the six active ingredients for treating the disease as designing weight coefficients, so that the optimum decocting conditions are optimized. Through the preparation method, the transfer rates of the six active ingredients in the formula for treating the disease, namely astragaloside, campanulin, puerarin, rhodioloside, isorhamnetin and ginsenoside Re, are improved to be equal to or more than 90%, and significantly higher than the transfer rates through various existing extracting methods at home; and pharmaceutical effect is improved and cost is reduced.

The invention relates to a method for preparing traditional Chinese medicine preparations, and particularly relates to a hyperthermic devitalization extraction method of a rhodiola rosea injection. Sofren injection has the effect of promoting blood circulation to remove stasis. According to the method, degrading enzyme in the rhodiola rosea medicinal material is inactivated in a short time in 80-90 DEG C high-temperature water by adopting an extracting technology of feeding materials in 80-90 DEG C high-temperature water, and salidroside is prevented from being decomposed by degrading enzyme in an aqueous solution in a process of heating cold water, and the product yield is improved.

The invention discloses a glucoside extract product used for lowering blood sugar and a production method thereof. The glucoside extract product comprises the following components in parts by weight: 25-32 parts of charantin, 12-15 parts of rhodioloside, 5-8 parts of triterpene sapogenin, 15-20 parts of naringin, and 8-10 parts of paeoniflorin. The production method comprises the following steps: extracting charantin, extracting rhodioloside, extracting triterpene sapogenin, extracting naringin, extracting paeoniflorin and producing a target glucoside extract product. The glucoside extract product and the production method have the advantages of wide application scope, multiple effective components, function complementation and mutual promotion of the effective components, and high extraction efficiency, and the product has the advantages of high purity and easy realization.