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Method for extracting salidroside from rhodiola rosea

A technology of salidroside and rhodiola, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, antidote, etc., can solve the problems of large amount of solvent, long extraction time, low extraction rate, etc. High strength, high extraction rate, and the effect of improving yield

Inactive Publication Date: 2014-12-03
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used extraction methods mainly include immersion or thermal reflux methods, which take a long time to extract, consume a large amount of solvent, have low extraction rates and are complicated to operate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use natural Rhodiola rosea as raw material, dry and pulverize, and pass through an 80-mesh sieve to obtain Rhodiola rosea powder. Take 500g of the powder and add 10 times PH4.0 sodium acetate / acetic acid buffer solution, cellulase and pectinase at a ratio of 1:1 , according to the amount of 0.3% of the raw material, carry out enzymolysis at 55 ° C for 3 hours; put the material after enzymatic conversion in microwave, the microwave power is 200W, the time is 90s, centrifuge, take the supernatant, concentrate, and obtain the crude extract; The obtained crude extract was dissolved in water, adsorbed and purified with macroporous adsorption resin AB-8, and then eluted with 60% ethanol 10 times of the crude extract, and the eluate was collected and concentrated under reduced pressure to obtain crude salidroside , recrystallized twice with methanol to obtain 5.4 g of pure rhodiola with a purity of 96.5%.

Embodiment 2

[0021] Take natural rhodiola rosea as raw material, dry and pulverize, cross 80 mesh sieves, get rhodiola rosea powder, get powder 500g and add 15 times of PH5.0 sodium acetate / acetic acid buffer solution, cellulase and hemicellulase with 1: 1. Carry out enzymatic hydrolysis at 45°C for 2 hours according to the dosage of 1.0% of the raw material; place the material after enzymatic conversion in a microwave with a microwave power of 250W for 120s, centrifuge, take the supernatant, concentrate, and obtain a crude extract The obtained crude extract is dissolved in water, adsorbed and purified with macroporous adsorption resin D-101, and then eluted with 80% ethanol 15 times of the crude extract, and the eluate is collected and concentrated under reduced pressure to obtain salidroside The crude product was recrystallized twice with absolute ethanol to obtain 5.2 g of pure Rhodiola rosea with a purity of 98.4%.

Embodiment 3

[0023] Use natural Rhodiola rosea as raw material, dry and pulverize, pass through 80 mesh sieve to get Rhodiola rosea powder, take 500g of the powder and add 12 times PH4.5 sodium acetate / acetic acid buffer solution, cellulase, hemicellulase and pectin Enzyme hydrolysis at 50°C for 2.5 hours at 1:1:1, according to the amount of 0.5% of raw materials; put the material after enzyme conversion in a microwave with a microwave power of 300W for 100s, centrifuge, and take the supernatant , concentrated to obtain a crude extract; the resulting crude extract was dissolved in water, adsorbed and purified with a macroporous adsorption resin HZ-816, and then eluted with 70% ethanol that was 12 times the crude extract, and the eluate was collected and decompressed Concentrate to obtain crude salidroside, recrystallize twice with absolute ethanol to obtain 5.5 g of pure rhodiola with a purity of 97.3%.

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Abstract

The invention relates to a method for extracting salidroside from rhodiola rosea. The method comprises the following treatment steps: by taking natural rhodiola rosea as a raw material, drying, grinding, allowing the rhodiola rosea to pass through a sieve with the granularity of 80 meshes, thereby obtaining rhodiola rosea powder; performing bio-enzyme enzymolysis; performing microwave assisted extraction; purifying through a macroporous resin; and crystallizing. According to the method disclosed by the invention, the rhodiola rosea is taken as the raw material, bio-enzyme enzymolysis is combined with microwave extraction, and cell walls of plants are destroyed, so that active ingredients in the cells are fully dissolved out, the extraction rate of the active ingredients is increased, and the method has the advantages of mild reaction conditions, high speed, low energy consumption, high quality, time conservation, no pollution and the like. Moreover, the microwave assisted extraction mode has the advantage that the content of protein impurities in the extracting solution is low and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant deep processing, and relates to a method for extracting salidroside from rhodiola rosea, in particular to a method for extracting salidroside by using enzymatic hydrolysis-microwave. Background technique [0002] Rhodiola belongs to the angiosperm phylum Rosaceae Sedum Rhodiola genus. It is a perennial herb or subshrub wild plant with rich resources and various varieties. In my country, it is mainly distributed in Northeast China, Gansu, Xinjiang, Sichuan, Tibet and Yunnan, Guizhou and other places. Rhodiola rosea has strong vitality and special adaptability, and belongs to natural and precious medicinal plants. Salidroside is the main active ingredient in Rhodiola Rosea, and it is also the most important indicator for evaluating Rhodiola Rosea and its extracts. Modern pharmacological studies have shown that salidroside has the effects of eliminating fatigue, anti-hypoxia, anti-tumor, anti-aging, a...

Claims

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Application Information

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IPC IPC(8): A61K36/41A61P39/00A61P39/06A61P35/00A61P17/16
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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