57 results about "FLUORESCENT IMMUNOASSAY" patented technology

A fluorescent immunoassay employing the interior surface of a capillary tube is provided. Devices to permit immunoassays using one or more capillary tubes, an apparatus for use with the devices, and a process for screening for analyte in a sample using the devices and apparatus are also provided. Samples suspected of containing analyte are added to a disposable self-contained sample tray containing one or more sample wells, mixed with a reagent, drawn into one or more spaced-apart capillary tubes held within a disposable cartridge connected to an analytical apparatus, reacted with a binding member on the surface of the capillary tube, washed to stop the reaction, and dried by the apparatus. The capillary tube is then exposed to a signal generation device to create a fluorescence signal that is detected using a signal detector. The apparatus determines the presence of the analyte and optionally determines the amount of analyte present in the sample, and presents the results to the operator.

The invention discloses a photoluminescent nano particle as well as a preparation method and an application thereof. The photoluminescent nano particle is composed of a carboxyl-containing copolymer matrix material and a rare earth fluorescent dye dispersed in the matrix material. The preparation method of the fluorescent nano particle comprises the following steps: dissolving the rare earth compound fluorescent dye and the copolymer in an organic solvent which is capable of being miscible with water; and adding the solution in water, so that the fluorescent nano particle is formed by utilizing a coprecipitation-self assembly process. The fluorescent nano particle has the advantages of excellent long wave excitation luminescence property and good stability, and has a surface carboxyl group for coupling biomolecules. A biological probe based on the fluorescent nano particle has a wide application prospect in the aspects of high-sensitivity fluorescent immunoassay, biological imaging and the like.

The invention relates to a double rare earth coordination compound, an Ag at SiO2 fluorescent nano particle doped with the same and a preparation method thereof. The fluorescent nano particle takes Ag doped with the double rare earth coordination compound Eu3<+> / Tb3<+>-PABA-DTPA-APTMS as an inner core; silicon dioxide with a mesh structure is covered on the surface of the inner core; an active amino group is arranged on the surface of the silicon dioxide, wherein the ratio of the double rare earth coordination Eu3<+> / Tb3<+>-PABA-DTPA-APTMS to the Ag is 1:0.176-0.2; and the mass ratio of the inner core to the silicon dioxide is 1:5-12 and each milligram of nano particle contains 595-630nmol of amino groups. The fluorescence intensity of Eu3<+> and Tb3<+> in the nano particle at the maximum transmitting peak is improved by 3.0 and 3.4 times compared with the fluorescence intensity of a SiO2 fluorescent nano particle doped with the Eu3<+> / Tb3<+>-PABA-DTPA-APTMS without an Ag core; the prepared nano particle is regularly spherical, is uniform in size with the particle size of 120+ / -5nm and has favorable monodispersity and light stability; and an amino group is arranged on the surface of the nano particle and directly reacts with a biomolecule without surface modification. The nano particle is expected to be used as a novel rare earth fluorescent probe which is applied to the time distinguishing fluorescent immunoassay for high-sensitivity detection, a biosensor, a biological chip and the like.

A fluorescent immunoassay employing the interior surface of a capillary tube is provided. Devices to permit immunoassays using one or more capillary tubes, an apparatus for use with the devices, and a process for screening for analyte in a sample using the devices and apparatus are also provided. Samples suspected of containing analyte are added to a disposable self-contained sample tray containing one or more sample wells, mixed with a reagent, drawn into one or more spaced-apart capillary tubes held within a disposable cartridge connected to an analytical apparatus, reacted with a binding member on the surface of the capillary tube, washed to stop the reaction, and dried by the apparatus. The capillary tube is then exposed to a signal generation device to create a fluorescence signal that is detected using a signal detector. The apparatus determines the presence of the analyte and optionally determines the amount of analyte present in the sample, and presents the results to the operator.

The invention discloses a fluorescent immunochromatography quantitative analyzer and a method. The analyzer includes a controller, a signal processing circuit and a fluorescent immunoassay optical detection module. The fluorescent immunoassay optical detection module comprises a light source, a first planoconvex lens, a silicon photocell, a dichroic mirror, a second planoconvex lens, an aperture diaphragm and a test strip. The controller is connected to a control terminal of the light source; the light emitted by the light source is reflected by the dichroic mirror, passes through the first planoconvex lens, and irradiates on an object to be measured on the test strip; the light irradiating on the object to be measured is divided into two parts, one part is absorbed by the object to be measured, and the other part of is reflected by the object to be measured, and passes through the first planoconvex lens, the dichroic mirror, the second planoconvex lens and the aperture diaphragm, and irradiates on a photosensitive surface of the silicon photocell; the signal output end of the silicon photocell is connected to a input end of the signal processing circuit; and the output end of the signal processing circuit is is connected with the input end of the controller. The invention can realize the quantitative detection of the fluorescent immunochromatography, and the cost is reduced.

The invention discloses a time-resolved fluorescent immunoassay kit for detecting glyphosate and a detecting method of the kit. The kit is composed of a porous coating plate, a buffer solution, a glyphosate standard product, a glyphosate antibody freeze-dried product, a europium-labeled goat-anti-mouse antibody, a washing solution and an enhancing solution. The method includes the following steps of firstly, preparing immunogen; secondly, preparing coating antigens; thirdly, preparing monoclonal antibodies; fourthly, preprocessing and detecting a sample. The kit is short in detecting time, high in average recovery rate, simple in sample preprocessing, capable of conducting site operation and detection, wide in application range and low in detection cost, and meanwhile has the advantages of being high in detection specificity, small in inside-batch and inter-batch difference, high in sensitivity, easy and rapid to operate, particularly suitable for detecting a large batch of samples and the like.

Disclosed are an automated card withdrawal mechanism and a single-channel fluorescent immunoassay instrument. The automated card withdrawal mechanism comprises a base plate (10), a slide rail (20), a sliding mechanism (30), a position blocking mechanism (40) and a card pushing mechanism (50), wherein the base plate (10) is provided with a long strip-shaped hollowed part (11), a card withdrawal slideway (12) is fixed under the base plate (10), a first end of the card withdrawal slideway (12) is provided with a slideway entrance enabling a test card (70) to go in and out; the slide rail (20) is mounted on the base plate (10) and is close to the hollowed part (11); the sliding mechanism (30) is movably mounted on the slide rail (20) and can move in the direction of the slide rail (20), and the test card (70) can be placed on the sliding mechanism (30); the position blocking mechanism (40) is fixed on the base plate (10) and at a position near a second end of the card withdrawal slideway (12); and the card pushing mechanism (50) is mounted on the sliding mechanism (30) and partially extends into the card withdrawal slideway (12), and can push the test card (70) out of the slideway entrance of the card withdrawal slideway (12), realising automated card withdrawal. The mechanism can realise active card pushing with reduced card withdrawal failure and high stability, and testing efficiency can be improved.

The invention discloses a handheld fluoroimmunoassay device. The device comprises a shell and an upper cover, the shell and the upper cover enclose an accommodating chamber, a fluorescence data acquisition module, an photoelectric scanning module and a master control module are arranged in the accommodating chamber, and the surface of the upper cover is provided with a touch screen; the fluorescence data acquisition module is arranged above the photoelectric scanning module, and the master control module is respectively connected with the fluorescence data acquisition module, the photoelectricscanning module and the touch screen; the device also comprises a pedestal bottom with a reagent card slot integrated to the photoelectric scanning module; and the position of the fluorescence data acquisition module in the accommodating chamber is moving. The fluorescence data acquisition module includes a plurality of excitation light sources which are light sources having different wavebands respectively, so multi-purpose use of one machine is realized, the detection of multiple fluorescent band samples can be achieved, and the application range of the device is enlarged; and the device adopting the moving fluorescence data acquisition module and fixed reagent card structure has the advantages of compact integral structure layout, small size, and easiness in realization of handheld operation and carrying.

A fluorescence immunoassay device based on integration of a photonic crystal and magnetic beads and a method thereof are provided. Magnetic beads with high surface-to-volume ratio are used as carriers of fluorescent molecules to obtain higher fluorescence density. The electric field on the surface of the photonic crystal is enhanced through excitation of photonic crystal resonance. The intensity of the fluorescence signal excited by the enhanced electric field is increased. Moreover, through interaction with the photonic crystal, some fluorescent signals that originally cannot be received by the fluorescent sensor are coupled to the photonic crystal resonant modes and reradiate toward the fluorescent sensor, thereby increasing collection efficiency. The fluorescence signals generated by fluorescent molecules on the magnetic beads are significantly intensified, which could lower the detection limit. Furthermore, the magnetic beads aggregation method can achieve the detection capability that cannot be achieved by the current fluorescent immunoassay.

An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; a step (b) of measuring the fluorescence intensity of the fluorescent dye; and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye.

The present invention belongs to the field of nano material and biological technology. The composite nano CdSe liposome microvesicle for fluorescent immunoassay is prepared through three steps of: preparing nano oily CdSe semiconductor crystal, synthesizing composite nano oily crystal microvesicle, and electrostatically connecting microvesicle with biological protein via altering pH value to regulate surface potential. The present invention is mainly used in test paper strip for immunochromatographic detection of hepatitis B virus, pesticide residue in vegetable and drug detection. The lipophilic surface nano CdSe crystal is phase transferred to constitute biocompatibility.

The invention relates to a method for judging the hook effect of homogeneous time-resolved fluorescence immunoassay. The fluorescence intensity ratio of two wavelengths is used as the signal value; (2) by measuring the signal values ​​at least two moments in the initial stage of the reaction, by linear fitting and four-parameter fitting, the absolute value of the signal intensity and the rate value of the signal change are respectively established. and the standard curve of the sample concentration; (3) detect the signal value of the unknown sample, and obtain the concentration of the unknown sample according to the fluorescence intensity of the unknown sample through the standard curve, thereby avoiding the hook effect. Using the ratio of the absolute value of the signal intensity and the value of the rate of change of the signal to the background signal, the relative signal intensity and the relative value of the rate of change of the signal are calculated to achieve consistent results on different instruments. The concentration interpretation of more samples can be completed within a given time, so that the system has higher detection throughput.

The invention discloses a PCT (Procalcitonin) and CRP (C-Reactive Protein) double-label time resolution fluorescence immunoassay method for simultaneously detecting bacterial meningitis and viral meningitis. A recombinant polypeptide antigen is included and the amino acid sequence is shown as SEQ ID NO: 1. The recombinant polypeptide antigen is used as an immunogen, and a PCT antibody and a CRP antibody are obtained by screening respectively through immune treatment; double-label time resolution fluorescence immunoassay is carried out. The method disclosed by the invention is high in sensitivity, is rapid, is simple and convenient and is easy to popularize clinically. The whole detection time of a kit utilizing the method is less than 2 hours, and suspected patients with the meningitis can be rapidly screened at an early stage. Reliable evidences are provided for specific treatment of children with the meningitis; pains of the children with the meningitis are alleviated and the diagnosis time of diseases is shortened; valuable time for the specific treatment is won, so that medical cost and a lot of manpower and material resource expenditures are saved, and worries and panics of parents are also alleviated.