The invention belongs to the technical fields of medical science and biochemistry, and concretely relates to an anti-cyclic citrullin peptide antibody detection kit. The kit comprises a reagent R1 anda reagent R2, and the reagent R1 includes a biological buffer agent, a coagulating accelerator, a surfactant, an osmotic pressure regulator and water; and the reagent R2 includes latex particles coated with the anti-cyclic citrullin peptide antigen, the biological buffer agent, a blocking agent, a preservative and water. Compared with traditional anti-cyclic citrullinated peptide antibody detection kits, the anti-cyclic citrullinated peptide antibody detection kit which uses latex-enhanced immunoturbidimetry to perform detection in the invention has the advantages of rapidity, sensitivity, good accuracy, high specificity and good stability.