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Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

One embodiment of the present invention includes a system comprising a housing having an upper end and a lower end. The housing carries a plurality of movable blades defining an entry aperture proximate to the upper end, an exit aperture proximate to the lower end, and a chamber there between. An adapter may be provided for connection to the housing and for receiving an article storage container. The upper end of the housing carries a sensor responsive to the presence and absence of the article storage container. A memory device carries information about the presence and absence of the article storage container. An article determining and actuating station has electronics for interrogating the memory device, for controlling the plurality of blades and for determining the number of items dispensed. A receptacle collects articles that have been dispensed. Methods of associating a flow control device with an article storage container and of dispensing items are also disclosed.

An acoustic array platform comprising multiple spaced microphones and associated processing electronics / algorithms projects distinct beams in a chosen look direction across which moving objects such as vehicles pass. These moving objects are accurately detected, classified and counted at extended ranges. The acoustic microphone array employs optimized beamforming to create specially focused listening directions that function as a “motion detector” and “trip line counter”. Algorithms detect and confirm the appropriate presence of objects moving through the beams and perform other algorithmic tests to verify that the object is a valid object to be counted. The proposed approach is realized with modest sized acoustic arrays and a reasonable number of microphones by employing an adaptive beamforming algorithm that achieves enhanced directivity in a principal look direction and which significantly reduces the effects of interferers outside the precise steering direction of the “trip line”.

A method for enumerating platelets within a blood sample is provided. The method includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; 2) admixing a colorant with the sample, which colorant is operative to cause the platelets to fluoresce upon exposure to one or more predetermined first wavelengths of light; 3) illuminating at least a portion of the sample containing the platelets at the first wavelengths; 4) imaging the sample, including producing image signals indicative of fluorescent emissions from the platelets, which fluorescent emissions have an intensity; 5) identifying the platelets by their fluorescent emissions, using the image signals; 6) determining an average fluorescent emission intensity value for the individual platelets identified within the sample; 7) identifying clumps of platelets within the sample using one or more of their fluorescent emissions, area, shape, and granularity; and 8) enumerating platelets within each platelet clump using the average fluorescent emission intensity value determined for the individual platelets within the sample.

An illumination control system includes lighting apparatuses connected to a communications line and a number of functional units of plural kinds functioning to detect information from ambient environments of the lighting apparatuses. Each lighting apparatus includes a lamp, an illumination circuit, a communication unit, a control unit, and a unit attaching part to which the functional unit is detachably attached. Each lighting apparatus belongs to one or more of plural groups corresponding to the functional units, the groups being operated based on information detected by the respective functional units. A functional unit having a detecting function corresponding to an operation of each group is attached to each unit attaching part of at least one lighting apparatus of each group, and each communication unit of the at least one lighting apparatus sends the control signal including information detected by the corresponding functional unit to another lighting apparatus in the same group.

The present invention provides an object measuring apparatus capable of performing high-speed process and accurately counting the number of a plurality of objects even in the case where the plurality of objects pass a boundary line simultaneously. The object measuring apparatus extracts motion vectors at a plurality of times in each of a plurality of positions on a boundary line on the basis of a plurality of images. The object measuring apparatus obtains at least one integral value by integrating components perpendicular to the boundary line of the motion vectors. As the at least one integral value, for example, an integral value derived by integrating the perpendicular components of one of positive and negative signs is obtained. The object measuring apparatus calculates the number of moving objects (people and the like) passing the boundary line on the basis of the integral value.

The invention provides a pedometer and a program. whenever an output signal of an acceleration transducer is detected to a reducing from an increasing(e.g. being a peak value), the peak value is compared with a first threshold value, and a time interval between the peak value and the detection timing of the previous peak value is compared with a second threshold value to judge whether to count the step number, and then, the detected peak value is compared with a third threshold value greater than the first threshold value, and a time interval between the peak value and the previous peak value is compared with a fourth threshold value representing a time shorter than the second threshold value to judge the action type of users(e.g. running, walking or other state). Under the situation of being judged as running or walking, the falling steep degree of a wave form after appearing a peak in an acceleration time waveform is estimated according to the judged result, and the step number is added up according to each action type.

A self-powered tire revolution counter includes a motion sensitive power generation mechanism, a power conditioner, a pulse detector, a microcontroller, and, optionally, a radio frequency (RF) transmitting device. In one exemplary embodiment, the power generation mechanism corresponds to a piezoelectric patch that, during movement, provides both operating electrical power and pulsed signals indicative of tire rotation. The power conditioner receives a generator signal from the power generation mechanism and produces a conditioned output voltage that can be used to power associated electronic devices, including the microcontroller. The pulse detector receives the generator signal and produces a detection signal whenever the generator signal meets a predetermined condition. The microcontroller is programmed to determine current and lifetime-accumulated values of selected pulse indications in the detection signal that meet predetermined criteria. Data corresponding to tire environment related parameters such as temperature, pressure, tire deflection, and / or vehicle speed may be stored in the microcontroller at times during tire rotation as power is supplied from the power generation mechanism through the power conditioner. Additional data may be supplied to the microcontroller directly from an external source and read from the microcontroller either by direct electrical contact or via selective RF transmission.

A small object dispenser adapted to receive a canister of objects such as pills, the canister being coupled atop it through a secure, bar-code matched gate operated by a central controller. A hopper below the gate directs smaller quantities of objects into a charge block adapted to measure out a select number of objects into an angularly disposed, circular counter where they accumulate atop a movable plate forming the bottom of the counter. The plate bears slots around its perimeter adjacent the cylindrical walls of the counter. As the central controller operates a servo motor to rotate the plate in measured increments, it urges a precise count of objects from the bottom of the counter to a port through which they fall one at a time into a receptacle such as a prescription bottle. Orientation means on the interior of the walls orient objects so that only a single object may fall into each slot, thereby preventing overfilling. A separate sensor counts the objects as they fall to verify quantity and guard against underfilling due to empty slots.

A system or method for counting the number of layers of a multilayer object is adapted for counting the number of layers by means of a simple arrangement of emitting an electromagnetic wave to strike the object that is in a multilayer state. In the system or method, an electromagnetic wave is caused to strike at least either the top surface or the bottom surface of a multilayer object and electromagnetic waves generated by reflection of the incident electromagnetic wave at the respective interfaces of the layers of the multilayer object or an electromagnetic wave generated by transmission of the electromagnetic wave through the multilayer object.

A method and an apparatus for enumerating one or more specific elements within a biologic fluid sample are provided. An embodiment of the method includes the steps of: a) providing a chamber formed between a first planar member that is transparent and a second planar member, which members are separated from one another by a substantially uniform height; b) introducing the biologic fluid sample into the chamber, wherein the chamber height is sized such that the sample extends between the first and second members, and sized relative to the specific elements within the sample such that the specific elements non-uniformly distribute within the sample upon introduction into the chamber; c) examining substantially all of the sample within the chamber and enumerating all of at least one of the specific elements; d) determining the volume of sample contained within the chamber; and e) determining the number of the at least one of the specific elements per unit volume.

Systems and methods for instance counting and for the identification of a temporal pattern in an event sequence. The system addresses the use of “earliest-first” and “no-reuse” policies as criteria for the correctness of counting. The system also achieves higher performance than conventional methods by utilizing incremental computation.

Described herein is a portable, low power consuming optical particle counter calibration verification system and reliable and sensitive methods for verifying the calibration status of a gas or liquid particle counter. The calibration verification systems described herein are useful for quickly determining the calibration status of an optical particle counter at its point of use, as well as for allowing the end user to determine if an optical particle counter is in need of a recalibration before the recommended calibration schedule suggests.

According to an aspect of the present invention, there is provided a people counting apparatus including: a reception unit which receives a video of an area including an entrance captured by a video capture device; a line setting unit which sets an inline at the entrance and sets an outline such that a specific region is formed on a side of the inline; a detection unit which detects moving objects in the video using information differences between frames of the received video and detects human moving objects among the detected moving objects; a tracking unit which tracks the movement of each of the detected moving objects; and a counting unit which determines whether each of the moving objects passed the inline and the outline based on the tracked movement of each of the moving objects and counts the number of people based on the determination result.

A step detecting apparatus and method in a personal navigator where acceleration signals in at least two directions are acquired from an accelerometer sensor, as a pedestrian moves. Variances of the acceleration signals are calculated and summed. The variance sum is compared with a threshold. If the variance sum is greater than the threshold, it is determined that a detected step is a walking step. If the variance sum is less than the threshold, it is determined that the detected step is a mark-time step.

A self-powered tire revolution counter includes a motion sensitive power generation mechanism, a power conditioner, a pulse detector, a microcontroller, and, optionally, a radio frequency (RF) transmitting device. In one exemplary embodiment, the power generation mechanism corresponds to a piezoelectric patch that, during movement, provides both operating electrical power and pulsed signals indicative of tire rotation. The power conditioner receives a generator signal from the power generation mechanism and produces a conditioned output voltage that can be used to power associated electronic devices, including the microcontroller. The pulse detector receives the generator signal and produces a detection signal whenever the generator signal meets a predetermined condition. The microcontroller is programmed to determine current and lifetime-accumulated values of selected pulse indications in the detection signal that meet predetermined criteria. Data corresponding to tire environment related parameters such as temperature, pressure, tire deflection, and / or vehicle speed may be stored in the microcontroller at times during tire rotation as power is supplied from the power generation mechanism through the power conditioner. Additional data may be supplied to the microcontroller directly from an external source and read from the microcontroller either by direct electrical contact or via selective RF transmission.

An information gathering system and method that utilizes a computer to authenticate, poll and store user responses to a customized questionnaire. The questionnaire can be a ballot. The computer utilizes various databases to authenticate and generate the customized questionnaire. The computer records user responses securely and anonymously. User and / or system errors are eliminated before finalizing the response database. An identification number is assigned to each user which allows the user to verify the integrity of the user's response by accessing a response database. The user is provided access to a tabulation database to allow the user to verify the user's voting choices and the voting choices of other voters.

The invention relates to the field of medical image processing and mode recognition, in particular to a tubercle bacillus target recognizing and counting algorithm based on diverse characteristics. The algorithm comprises the following steps of: image preprocessing: carrying out image reinforcement and constructing median filter and Gaussian filter on a tubercle bacillus microimage; color image partition: carrying out fixed threshold partition based on HSV (Hue-Saturation-Value) color space on a preprocessed image and then carrying out adaptive threshold partition which is based on CIE L*a*b* color space and keeps a geometric shape of a target; communication block morphological analysis and target recognition: carrying out communication block analysis on the partitioned image; and tubercle bacillus target counting: estimating the quantity of tubercle bacillus targets in the image by utilizing a histogram statistics and multistrategy calculation method. The invention can effectively extract the bacillus targets in the tubercle bacillus microimage subjected to acid-fast stain from background and impurities and carry out accurate counting, thereby realizing the automation and the intellectualization of the detection of tubercle bacilli.

The present invention discloses a method of counting the number of MBMS subscribers, comprising: setting different types of notifications, each of which is designed to instruct UEs in at least one mode to respond said counting number of subscribers; the network side issuing different types of notifications, so as to monitor the activation of UEs in at least one mode in the MBMS with said notifications, and thereby achieving counting the number of subscribers of each MBMS. The method of counting the number of MBMS subscribers provided in the present invention can be used to perform counting the number of subscribers of each MBMS more accurately, so as to take fuller advantage of the precious air interface resource.

An intelligent irrigation rain sensor for use in an irrigation system comprises a rain sensor unit and a sensor control unit. The rain sensor unit has a rain catcher reservoir with an open top for receiving rainwater. The rain catcher has an internal volume for holding water and tapers into a funnel with an opening for metering equally sized round water droplets at a steady-state drip rate. At least one droplet detector is positioned directly below the funnel opening that contacts the water droplets from the funnel. The droplet detector includes detection electrodes for sensing a change in an electrical property being monitored by a detection control in the sensor control unit. Each droplet detected may be counted and the droplet count retained in a memory, or excluded from counting based on certain factors, such as the drip rate between droplets from the funnel opening. Upon exceeding a rain threshold amount over measurement time period, the intelligent irrigation rain sensor issues a rain signal to the irrigation controller. The intelligent irrigation rain sensor issues a corresponding dry signal to the irrigation controller after a drying period has elapsed. The present intelligent irrigation rain sensor may issue consecutive rain signals and accumulate drying periods for each, up to a predetermined limit, before issuing a single dry signal to the irrigation controller.

This invention is to a wireless dual module system for sensing and indicating the remaining rounds contained within a detachable or integrated firearm magazine. The dual module system, one containing a display (display module) and one more round sensing modules (magazine module) may be implemented with no significant modifications to the firearm or magazine. The display module and one or more magazine modules wirelessly linked together are referred to as the system. The system is configured to provide the number of rounds remaining in a magazine, if a magazine is seated properly in the magazine, to indicate an empty magazine, or to indicate that the number of rounds remaining in the magazine is above or below a predetermined range. The system is able to provide an immediate indication of the remaining rounds in a magazine, regardless of the amount of rounds discharged or supplemented by the user.

The invention relates to a hemolytic agent for blood leukocyte five-classification counting and an application thereof. The hemolytic agent comprises a hemolytic agent A and a hemolytic agent B. The hemolytic agent A comprises a polyoxyethylene nonionic surfactant, an organic acid, a quaternary ammonium salt cationic surfactant cosolvent, a stabilizing agent and an osmotic regulator; the hemolytic agent B comprises alkaline inorganic salt and an osmotic regulator. When compared with the result detected by a standard hemolytic agent produced by BeckmanCoulter company, the hemolytic agent of the invention has good correlation and high accuracy. Especially, when abnormal cells exist in a blood specimen, the hemolytic agent has high warning sensitivity and good specificity, and exhibits the most beneficial effect.

A method for enumerating platelets within a blood sample is provided. The method includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; 2) admixing a colorant with the sample, which colorant is operative to cause the platelets to fluoresce upon exposure to one or more predetermined first wavelengths of light; 3) illuminating at least a portion of the sample containing the platelets at the first wavelengths; 4) imaging the sample, including producing image signals indicative of fluorescent emissions from the platelets, which fluorescent emissions have an intensity; 5) identifying the platelets by their fluorescent emissions, using the image signals; 6) determining an average fluorescent emission intensity value for the individual platelets identified within the sample; 7) identifying clumps of platelets within the sample using one or more of their fluorescent emissions, area, shape, and granularity; and 8) enumerating platelets within each platelet clump using the average fluorescent emission intensity value determined for the individual platelets within the sample.

A method and device are provided for counting cells in a sample of living tissue, such as an embryo. The method involves obtaining a microscopic image of the unstained tissue that reveals cell boundaries, such as a differential interference contrast (DIC) image, and an optical quadrature microscopy (OQM) image which is used to prepare an image of optical path length deviation (OPD) across the cell cluster. The boundaries of individual cells in the cell cluster are modeled as ellipses and used, together with the maximum optical path length deviation of a cell, to calculate ellipsoidal model cells that are subtracted from the OPD image. The process is repeated until the OPD image is depleted of phase signal attributable to cells of the cell cluster, and the cell count is obtained from the number of cells subtracted. The method is capable of accurately and non-invasively counting the number of cells in a living embryo at the 2-30 cell stage, and can be employed to assess the developmental stage and health of human embryos for fertility treatments.

A tablet inspection apparatus includes: a transmitted illumination unit configured to emit infrared light to a medicine packet made of two films from a side of the films; a reflected illumination unit configured to emit infrared light from the other side of the two films; a camera unit configured to obtain a transmission image by imaging the medicine packet, in a state where the transmitted illumination unit is emitting infrared light and the reflected illumination unit is not emitting infrared light, and obtain a reflection image by imaging the medicine packet, in a state where the reflected illumination unit is emitting infrared light and the transmitted illumination unit is not emitting infrared light; and a calculation unit configured to generate an inspection image from a difference image between the reflection image and the transmission image.

Pedestrian detection and counting for traffic intersection control analyzes characteristics of a field of view of a traffic detection zone to determine a location and size of a pedestrian area, and applies protocols for evaluating pixel content in the field of view to identify individual pedestrians. The location and size of a pedestrian area is determined based either on locations of vehicle and bicycle detection areas or on movement of various objects within the field of view. Automatic pedestrian speed calibration with a region of interest for pedestrian detection is accomplished using lane and other intersection markings in the field of view. Detection and counting further includes identifying a presence, volume, velocity and trajectory of pedestrians in the pedestrian area of the traffic detection zone.