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Hemolytic agent for blood leukocyte five-classification counting and application thereof

A technology of hemolytic agent and white blood cell, applied in the field of blood analysis, to achieve the effect of strong ability to lyse red blood cells and high stability

Inactive Publication Date: 2011-10-26
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still uses saponin, so there is also the problem of saponin as a hemolytic agent as mentioned above

Method used

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  • Hemolytic agent for blood leukocyte five-classification counting and application thereof
  • Hemolytic agent for blood leukocyte five-classification counting and application thereof
  • Hemolytic agent for blood leukocyte five-classification counting and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1: Preparation of hemolytic agent for five-differential counting of blood leukocytes of the present invention

[0100] Preparation of hemolytic agent A:

[0101] Use an analytical balance to accurately weigh out 3.0 parts by weight of polyoxyethylene sorbitan monolaurate, 5.0 parts by weight of formic acid, 1.0 parts by weight of tetradecyltrimethylammonium chloride, 5.0 parts by weight of EDTA potassium salt, and 1.0 parts by weight. Parts of sodium chloride. Add the above-mentioned tetradecyltrimethylammonium chloride and EDTA potassium salt in the Erlenmeyer flask, and then add an appropriate amount of redistilled water (water resistance ρ>10MΩ / cm) at a temperature of 40℃ to dissolve; then add the above Stir the polyoxyethylene sorbitan monolaurate, dissolve and cool to 20℃, add organic acid formic acid to adjust the pH to 2.90±0.1, and finally add the pre-dissolved sodium chloride solution to adjust its osmotic pressure to 60± 5mOsm / Kg.

[0102] Preparation of h...

Embodiment 2

[0105] Example 2: Preparation of hemolytic agent for five-differential counting of blood leukocytes of the present invention

[0106] Preparation of hemolytic agent A:

[0107] Weigh 10.0 parts by weight of polyoxyethylene sorbitan monopalmitate, 1.0 parts by weight of citric acid, 5.0 parts by weight of dodecyltrimethylammonium bromide, 1.0 parts by weight of EDTA potassium salt, and 4.0 parts by weight with an analytical balance. Parts by weight of potassium chloride. In the Erlenmeyer flask, first add the above-mentioned dodecyltrimethylammonium bromide and EDTA potassium salt, and then add an appropriate amount of double distilled water (water resistance ρ> 10MΩ / cm) at a temperature of 42 ℃ to dissolve; then add poly Stir the oxyethylene sorbitan monopalmitate, dissolve and cool to 24℃, add organic acid citric acid to adjust the pH to 4.5±0.1, and finally add the pre-dissolved potassium chloride solution to adjust its osmotic pressure to 100±3mOsm / Kg.

[0108] Preparation of ...

Embodiment 3

[0111] Example 3: Preparation of hemolytic agent for five-differential counting of blood leukocytes of the present invention

[0112] Preparation of hemolytic agent A:

[0113] Weigh 8.0 parts by weight of polyoxyethylene sorbitan monostearate, 1.5 parts by weight of acetic acid, 4.1 parts by weight of cetyltrimethyl ammonium chloride, 1.6 parts by weight of EDTA sodium salt, and 3.5 parts by weight with an analytical balance. Parts by weight of sodium chloride. In the Erlenmeyer flask, first add the above-mentioned cetyltrimethylammonium chloride and EDTA sodium salt, and then add an appropriate amount of double distilled water (water resistance ρ>10MΩ / cm) at a temperature of 50°C and stir to dissolve; then add poly Stir the oxyethylene sorbitan monostearate, dissolve and cool to 25℃, add organic acid acetic acid to adjust the pH to 2.0±0.1, and finally add the pre-dissolved sodium sulfate solution to adjust its osmotic pressure to 80±3mOsm / Kg.

[0114] Preparation of hemolytic ...

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Abstract

The invention relates to a hemolytic agent for blood leukocyte five-classification counting and an application thereof. The hemolytic agent comprises a hemolytic agent A and a hemolytic agent B. The hemolytic agent A comprises a polyoxyethylene nonionic surfactant, an organic acid, a quaternary ammonium salt cationic surfactant cosolvent, a stabilizing agent and an osmotic regulator; the hemolytic agent B comprises alkaline inorganic salt and an osmotic regulator. When compared with the result detected by a standard hemolytic agent produced by BeckmanCoulter company, the hemolytic agent of the invention has good correlation and high accuracy. Especially, when abnormal cells exist in a blood specimen, the hemolytic agent has high warning sensitivity and good specificity, and exhibits the most beneficial effect.

Description

【Technical Field】 [0001] The invention belongs to the technical field of blood analysis. More specifically, the present invention relates to a hemolytic agent for five-differential counting of blood white blood cells, and also relates to the use of the hemolytic agent. 【Background technique】 [0002] In clinical hematology tests, counting and classifying peripheral blood white blood cells of patients is of great significance for the diagnosis and treatment of many diseases. The use of a fully automated blood analyzer for white blood cell classification usually requires the use of a hemolytic agent. On the one hand, the hemolytic agent can dissolve red blood cells and release hemoglobin in the cells for the determination of hemoglobin. On the other hand, the hemolytic agent can eliminate the interference of red blood cells and red blood cell fragments on the analysis of white blood cells after destroying red blood cells, so that the subsequent white blood cell count and classifi...

Claims

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Application Information

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IPC IPC(8): G01N33/50
Inventor 乐家新
Owner GENERAL HOSPITAL OF PLA
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