46results about How to "Fluorescent properties are stable" patented technology

The invention provides an establishment method based on a surface plasma reinforcing energy transferring biosensor. The establishment method comprises the following steps of: 1) preparing a fluorescent gold nanoparticle cluster wrapped with protein; 2) preparing the gold nanoparticle modified by mercaptoethylamine; and 3) establishing an experiment system based on the surface plasma reinforcing energy transferring biosensor. The establishment method based on the surface plasma reinforcing energy transferring biosensor provided by the invention has the advantages that the fluorescent gold nanoparticle cluster wrapped with the protein and the gold nanoparticle modified by mercaptoethylamine are introduced to be served as the donor and the receptor for energy transferring; the concept of surface plasma reinforcing energy transfer is utilized to achieve quick, accurate, high-sensitivity and high-selectivity quantifying of the object liquaemin; synthesizing of the nanometer material in the method is simple in steps, removes harsh equipment and condition, is safe, simple and convenient to operate, low in toxicity, and low in cost; and the used synthesizing and detecting instrument and equipment are common equipment; and a reaction condition is simple; and the reaction can be simply carried out at a room temperature of water bath at 37 DEG C.

The invention belongs to the technical field of preparation of carbon quantum dot materials, and relates to a preparation method for solid-state carbon quantum dots. The preparation method comprises the following steps: firstly, mixing an ionic liquid with nitrile substances, and putting the mixture, as an electrolytic solution, into an electrolytic tank; respectively selecting two platinum electrodes, of which the area is no smaller than 1.0 cm<2>, as an anode and a cathode, and putting the anode and the cathode into the electrolytic tank, and switching on a power supply to react, thereby obtaining a light brown solution mixed with solid-state substances; then, carrying out suction filtration on the light brown solution mixed with the solid-state substances, carrying out vacuum-drying on the solid substances obtained by suction filtration, and transferring the solid substances into a clean container vessel, thereby obtaining the solid-state carbon quantum dots. The preparation method is simple in preparation process, convenient in operation, accessible in principle, and low in cost; the prepared solid-state carbon quantum dots are good in water solubility, stable in fluorescent property, and easy to store and transport.

The invention relates to a germanium fluorescent cluster, a preparation method of the germanium fluorescent cluster in aqueous solution and its application in biological imaging. The synthesis method uses germanium dioxide as germanium source, biomolecular cysteine ​​as protective agent, and sodium borohydride as reducing agent to prepare monomolecular-layer protected germanium nanoclusters with fluorescent properties. The raw materials used in the present invention are simple, easy to obtain, stable in nature, and non-toxic; the reaction process does not require the protection of high temperature and inert gas, and is simple and easy to operate; the obtained germanium quantum dots with emerald green or yellow fluorescence are in phase with the previously reported noble metal nanoclusters. It has a higher fluorescence quantum yield, and the cluster size is about 1nm. It has good water solubility and no cytotoxicity, and can be well applied to biological cell imaging.

The invention provides a method and product for preparing autofluorescence polystyrene material, which is characterized in that on the surface of polystyrene material, especially on the surface of polystyrene microspheres, it is acylated by Friedel-Crafts under the action of an acidic catalyst (Alkylation) reaction introduces small molecule color-producing (color-aiding) functional groups, which form π-π (p-π) conjugation with the large π bond of the benzene ring to form a conjugated system, which undergoes electronic transition under the action of excitation light Produce fluorescence. Under the excitation of 405nm laser, the prepared polystyrene fluorescent microspheres can produce obvious fluorescent signals in the blue, green and orange channels of the laser confocal microscope. At the same time, we found that the introduction of electron-withdrawing groups on the conjugated substituents can red-shift the emission peak of polystyrene materials. The preparation method of the invention is simple, high in efficiency and low in cost. The prepared autofluorescent polystyrene microspheres have the characteristics of high fluorescence intensity, stable chemical properties, no fluorescence leakage, high mechanical strength, etc., and have great application potential in the fields of biomedicine and electronic light-emitting devices.

The invention discloses a precious metal / phenolic resin core-shell structure biocompatible material and the preparation method and is characterized in that phenol, hexamethylenetetramine and the salt solution of precious metal are reacted for 2 to 10 hours at 120 to 180 DEG C so as to obtain a core-shell structure particle; and then the core-shell structure particle is used as the carrier and reacted for 5min-10h at 160-200 DEG C through the method of synthesizing magnetic nano-Fe3O4 to synthesize a three-tier core-shell structure particle containing Fe3O4 modified precious metal / phenolic resin / Fe3O4, or synthesize a four-tier core-shell structure material particle containing gold nano-particle modified precious metal / phenolic resin / Fe3O4 / Au. The material has fluorescent and magnetic dualfunctions and has good application prospect in biological fluorescence imaging or magnetic resonance imaging technology in living biological organisms or cells. The preparation method of the invention can easily obtain the raw materials and has simple technology and easy operation, which is suitable for industrialization amplification.

According to the cyclic amidinyl fluorescent molecule with the same-side push-pull electron effect described in the present invention, the structural formula of the fluorescent molecule is: the cyclic amidinyl fluorescent molecule contains both an electron-pushing group (EDG) and an electron-withdrawing group (EWG). ), thereby constructing a novel skeleton molecule with a 10π electron structure, in which the electron-pushing group and the electron-withdrawing group are on the same side of the π electron, and this structure has a large Stokes shift (approximately 100nm), excellent acid-base stability, and stable fluorescent properties in the pH range of 2.8-11.4. At the same time, the fluorescent molecule has no obvious cytotoxicity and has broad biological application prospects. A preparation method of the fluorescent molecule is also disclosed. The method has simple process, mild conditions and is suitable for industrial batch production.

The invention discloses a method for measuring cellular oxidative stress damages under ionizing radiation by using fluorescent protein as a fluorescence probe. The content of free radicals generated in ionizing radiation is quantitatively measured by using the fluorescent protein which is selectively positioned and expressed on a subcellular structure sensitive to the ionizing radiation, so that the damages of the ionizing radiation on the specific positions of a cell are speculated by directly observing the fluorescence intensity change of the protein. By the method, the emitted fluorescence is easy to detect, has high sensitivity, stable fluorescence properties, can normally emit light in a wider pH range, has higher resistance to high temperatures, detergents, salt, organic solvents and most ordinary enzymes, is not toxic to the cell, is not interfered by false positive, makes vector construction convenient and is expressed without specificities of species, tissues and positions.