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Preparation method and application of ratio-type CNQDs/TiO2/AuNCs composite fluorescent microsphere

A technology of composite fluorescence and fluorescent microspheres, which is applied in the field of photochemical detection, can solve the problems of poor technical repeatability and cumbersome technical operation, and achieve the effects of improving sensitivity and specificity, expanding application range, and large specific surface area

Active Publication Date: 2019-01-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of existing technologies, such as some technical operations are cumbersome and some technologies have poor repeatability, etc.

Method used

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  • Preparation method and application of ratio-type CNQDs/TiO2/AuNCs composite fluorescent microsphere
  • Preparation method and application of ratio-type CNQDs/TiO2/AuNCs composite fluorescent microsphere
  • Preparation method and application of ratio-type CNQDs/TiO2/AuNCs composite fluorescent microsphere

Examples

Experimental program
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Effect test

Embodiment 1

[0041] S1: Preparation of CNQDs solution;

[0042] The specific steps for preparing CNQDs are as follows: firstly, 10 g of melamine was weighed and calcined in a muffle furnace at 600 °C for 2 h. get the yellow C 3 N 4 powder; secondly, 30 mg of C 3 N 4 The powder was dissolved in 3 g PEG 200, and 1.5 g concentrated KOH (1.1 g / mL) was added; after stirring for 15 min, the solution was transferred to a reaction kettle, and reacted at 180 ° C for 16 h; after cooling to room temperature, the reaction solution was used An organic membrane with a pore size of 0.22 μm was used for suction filtration, and the collected filtrate was dialyzed in a dialysis bag with a molecular weight cut-off of 1000 for 24 hours, and the dialyzed solution was distilled to 100 mL with distilled water for use.

[0043] S2: Preparation of CNQDs / TiO 2 Ethanol solution of fluorescent microspheres;

[0044] 0.15 mL of tetrabutyl titanate was gradually added to 10 mL of ethylene glycol and kept stirring...

Embodiment 2

[0062] S1: Preparation of CNQDs solution;

[0063] The specific steps for preparing CNQDs are as follows: firstly, 10 g of melamine was weighed and calcined in a muffle furnace at 600 °C for 2 h. get the yellow C 3 N 4 powder; secondly, 30 mg of C 3 N 4 The powder was dissolved in 1.5 g PEG 200, and 0.3 g concentrated KOH (1.1 g / mL) was added; after stirring for 15 min, the solution was transferred to a reaction kettle, and reacted at 180°C for 16 h; after cooling to room temperature, the reaction solution was Suction filtration was performed with an organic membrane with a pore size of 0.22 μm, and the collected filtrate was dialyzed in a dialysis bag with a molecular weight cut-off of 1000 for 24 h, and the dialyzed solution was distilled to 100 mL with distilled water for use.

[0064] S2: Preparation of CNQDs / TiO 2 Ethanol solution of fluorescent microspheres;

[0065] 0.1 mL of tetrabutyl titanate was gradually added into 10 mL of ethylene glycol and stirred at room...

Embodiment 3

[0077] S1: Preparation of Carbon Nitride Fluorescent Quantum Dots (CNQDs)

[0078] The specific steps for preparing CNQDs are as follows: firstly, 10 g of melamine was weighed and calcined in a muffle furnace at 600 °C for 2 h. get the yellow C 3 N 4 powder; secondly, 30 mg of C 3 N 4 The powder was dissolved in 30 g PEG 200, and 3 g concentrated KOH (1.1 g / mL) was added; after stirring for 15 min, the solution was transferred to a reaction kettle, and reacted at 180 ° C for 16 h; after cooling to room temperature, the reaction solution was Suction filtration was performed with an organic membrane with a pore size of 0.22 μm, and the collected filtrate was dialyzed in a dialysis bag with a molecular weight cut-off of 1000 for 24 h, and the dialyzed solution was distilled to 100 mL with distilled water for use.

[0079] S2: Preparation of CNQDs / TiO 2 fluorescent microspheres

[0080] 0.1 mL of tetrabutyl titanate was gradually added into 50 mL of ethylene glycol and stirr...

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Abstract

The invention belongs to the technical field of photochemical detection and relates to a preparation method and application of a ratio-type CNQDs / TiO2 / AuNCs composite fluorescent microsphere. The preparation method concretely comprises the following step of firstly, preparing a CNQDs solution, a CNQDs / TiO2 fluorescent microsphere and the ratio-type CNQDs / TiO2 / AuNCs composite fluorescent microsphere. Then, the application comprises the following step of utilizing the ratio-type CNQDs / TiO2 / AuNCs composite fluorescent microsphere to perform trypsin detection. The detection step comprises the following step of adding a trypsin standard solution in an aqueous ratio-type CNQDs / TiO2 / AuNCs composite fluorescent microsphere solution, acquiring fluorescent pictures and fluorescence spectrum curves at different concentrations, constructing a visual array and a standard curve for detecting trypsin, and realizing visual and quantitative detection of the trypsin in a sample. The preparation method and the application provided by the invention are simple in technology, efficient and rapid in detection method, expand the application range of the fluorescent microsphere at the same time and have awide application prospect.

Description

technical field [0001] The invention belongs to the technical field of photochemical detection, in particular to a ratio-type CNQDs / TiO 2 Preparation method of / AuNCs composite fluorescent microspheres and its application in trypsin detection. Background technique [0002] Trypsin, formed in the pancreas, can hydrolyze amino acid compounds connected by peptide chains, and can effectively digest protein. It is one of the most important digestive enzymes in the human body. Impaired trypsin production hinders the digestive process, and the production of trypsin outside of pancreatic tissue may be involved in the cancer process. There is basically no trypsin in the urine of normal people, but the trypsin content in patients with acute pancreatitis and chronic renal failure will increase significantly, and more than half of the patients with pancreatic cancer and chronic pancreatitis will also increase the trypsin content. Therefore, simple and timely monitoring of trypsin cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/65
CPCC09K11/65G01N21/64G01N21/6428G01N21/6456G01N2021/6421G01N2021/6432
Inventor 邹小波胡雪桃石吉勇史永强李文亭张文黄晓玮李志华徐艺伟
Owner JIANGSU UNIV
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