The present invention relates to a
coumarin compound that is used for analysis of enzymatic activity and
enzyme inhibitor screening. The compound has a structure formula as shown in the right; wherein, R<1> is an H atom, ester prepared from
hydrogen of OH that is substituted by aliphatic carboxyl or aromatic
carboxylic acid, or
ether that is prepared from
hydrogen of OH that is substituted by carboxyl of
sterol,
monosaccharide and
polysaccharide; R <2>, R<3> and R<4> can be H,
alkyl containing 1 to 18 carbon atoms, perfluorinated
alkyl containing 1 to 18 carbon atoms, -CN,-Ar, miscellaneous
aryl, carbonyl or carboxyl
amide. The
coumarin compound adopts Pechmann reaction or Knovenagel reaction to introduce
halogen atoms in the sixth and eighth positions of a
benzene ring, to prepare the di-halogenated 7-hydroxycoumarin compound. The
coumarin compound can be used as a fluorescent probe for marking analysis in the biotechnological research, and has the characteristics of simple operation, high stability, high sensitivity,
high selectivity, and so on. And the
coumarin compound is mainly used for the analysis of enzymatic activity, the
enzyme inhibitor screening, and the detection of
cell activity.