74 results about "Aminocoumarins" patented technology

The invention provides a method for detecting coumarin series compounds, which comprises the following steps that: ultra high-efficiency liquid chromatography and tandem mass spectrometry detection are carried out to a sample, so as to determine the types and the content of coumarin series compounds contained in the sample. The coumarin series compounds comprise one or more types in vinegar Glass coumarin, dicoumarol, 6-methoxy-7-hydroxy coumarin, 7-hydroxycoumarin, 8-hydroxy-psoralen, 4-methyl-7-hydroxy coumarin, 6,7-dimethoxy coumarin, coumarin, 7-methoxy coumarin, dihydro coumarin, psoralen, psoralen, 8-methoxypsoralen, 7-methyl, 5-methoxypsoralen, 4-methyl-7-ethoxyl coumarin, 4-methyl-7-diethylamino coumarin, 2', 4,8-trimethyl psoralen, imperatorin, isoimperatorin, columbianadin and cyclocoumarol. The method of the invention can complete the analysis and the detection of 22 coumarin series compounds within 4 min.

The invention discloses a synthetic method for thiocoumarin, and relates to the field of organic synthesis. The synthetic method for thiocoumarin comprises the following steps: taking C-4-positioni amino substituted coumarin and organic thiosulfate as raw materials; and reacting for (18-30) h at the temperature of (40-100) DEG C through iodine catalysis to obtain a C-3-position sulfur substitutedcoumarin compound. The invention provides a novel method for synthesizing an amino coumarins derivative with potential anti-cancer activity. Raw materials are economical and easily obtained, reactionconditions are green, low-toxicity and odorless, and the synthetic method for thiocoumarin has the advantages of gentle reaction conditions, low cost, small environmental pollution, high yield and thelike.

The invention discloses a synthetic method of a coumarin-pyrrole compound. The synthetic method comprises the following step of: in a reactive solvent, with 4-amino coumarin compounds and disubstituted acetylene compounds as raw materials, under the catalysis of palladium (II) and action of an oxidant, carrying out a reaction to obtain the coumarin-pyrrole compound. The synthetic method is mild in reaction conditions, simple and convenient in route and high in atom economy and has a favorable industrial application prospect; and raw materials are low in cost and easy to obtain.

The invention relates to preparation and application of a pyridine methylene coumarin copper ion fluorescent probe. The chemical name of the fluorescent probe is 7-diethyl amino-3-(pyridine-2-methylene) aminocoumarin; the preparation of the fluorescent probe comprises the steps that (1) 3-amino-7-diethyl aminocoumarin and 2-pyridylaldehyde are dehydrated under the action of a dehydrating agent toobtain an intermediate imine compound; (2) the intermediate imine compound is reduced under the action of a reducing agent to obtain a target fluorescent molecular probe compound. The application of the fluorescent probe is to detect whether or not divalent copper ions exist by utilizing copper ions to quench the fluorescence of a molecular fluorescent probe compound. The preparation and application of the pyridine methylene coumarin copper ion fluorescent probe have the advantages that the preparation of the probe is simple, the reaction conditions are mild, the after-treatment process is relatively simple; and fluorescent probe molecules show higher selectivity and sensitivity in the detection process of the bivalent copper ions.

An aminocoumarin conjugated to a fluorescent label through a secondary amine, is operative as a fluorescent polarization probe of the DNA gyrase B or topoisomerase IV E subunit. The probe is used for detecting topoisomerase inhibitor binding by fluorescence polarization, particularly in a high-through put topoisomerase inhibitor assay.

The invention provides a fluorescent probe HM, and a preparation method and application thereof. The fluorescent probe is 7-(diethylamino)-3-[3-(2-hydroxy-5-methoxyphenyl)-3-oxo-1-propenyl]coumarin. The preparation method comprises the following steps: dissolving 7-(diethylamino)coumarin-3-formaldehyde and 1-(2-hydroxy-5-methoxyphenyl)ethanone in acetonitrile according to an equal molar ratio, dropwise adding piperidine, carrying out heating reflux for 20 hours, cooling to room temperature, carrying out reduced pressure suction filtration, collecting filter residues, and carrying out vacuum drying to obtain a product which is a red solid. The fluorescent probe is enhanced in detection of ClO<->, shows high sensitivity and good selectivity and stability, and has the advantages of simple and rapid detection process, accurate detection result and the like. In addition, by combining a laser confocal scanning microtechnique and a biological living body imaging technique, the novel fluorescent probe is successfully applied to detection of ClO<-> in cells and liver cancer mouse models.

The invention relates to a ratio type probe for detecting mercury ions and a preparation method and application thereof. Specifically, the invention provides a probe for detecting mercury ions, which is simple and convenient to operate and high in sensitivity. The name of the probe is 7-diethylamino-3-(3-(7-diethylamino) coumarin)-3-oxopropenyl quinoline-2-ketone, and the probe is called QCT-O for short. The QCT-O is obtained by taking 7-diethylamino-2-oxo-1, 2-dihydroquinoline-3-formaldehyde and 7-diethylaminocoumarin-3-ethanone as raw materials, and carrying out a reaction under the action of a catalyst. The probe has the advantages of fast response to mercury ions, strong selectivity and high sensitivity. In the presence of mercury ions, the color of the probe solution changes from red to yellow (under sunlight), and changes from red fluorescence to blue-green fluorescence (under an ultraviolet lamp); the absorption peak disappears at the position of 500 nm, and new absorption peaks appear at the positions of 380 nm and 440 nm; the fluorescence intensity obviously changes at the wavelength of 650 nm, the absorption and emission response intensities linearly changes with the mercury ion concentration, and the lowest detection limits are 2.62 * 10 <-8 > mol/L and 4.37 * 10 <-8 > mol/L respectively.

The invention discloses a novel coumarin conjugated heterocyclic fluorescent probe for detecting Fe (III); phenanthroline is used as a receptor; coumarin with good photophysical and photochemical properties is used as a chromogenic group; the novel fluorescent probe 3-(2-(1,10-phenanthrolin-3-yl)ethynyl)-7-(diethylamino)-2H-coumarin (M3) is synthesized; the preparation method specifically comprises the step of synthesizing 3-bromo-7-diethylaminocoumarin, 3-(2-(trimethylsilyl)ethynyl)-1,10-phenanthroline, 3-ethynyl-1,10-phenanthroline and a phenanthrolinyl coumarin compound M3. The organic compound M3 has strong fluorescence, is a fluorescent probe capable of rapidly, efficiently and specifically identifying Fe<3+> in an aqueous solution, and has quite good application prospects, the synthesis process is simple, the reaction conditions are easy to control, and the yield is high.

The invention belongs to the technical field of pesticides, and particularly relates to an indole spiropyridino coumarin compound as well as a preparation method and application thereof. According to the present invention, the isatin derivative, the 1, 3-dicarbonyl compound and the 4-aminocoumarin are adopted as the raw materials, the indole spiropyridino coumarin compound is synthesized through the one-pot method, and the indole spiropyridino coumarin compound has characteristics of unique chemical structure, higher research value and higher application potential. The preparation method provided by the invention has the advantages of cheap raw materials, simple process and strong universality, and is suitable for industrial production. Particularly, the method for preparing the indole spiropyridino coumarin compound by taking water as a solvent has the characteristics of being green, safe, efficient and environment-friendly. The indole spiropyridino coumarin compound provided by the invention has good bacteriostatic activity, has inhibitory activity on common plant pathogenic bacteria such as peanut brown spots, apple ring spots, wheat sheath blight, watermelon anthracnose and rice seedling, and has a potential application value.

The invention provides a novel fluorescent probe capable of simultaneously and quantitatively detecting Cys, Hcy and GSH in plasma as well as a preparation method and application of the novel fluorescent probe. The preparation method comprises the following steps: preparing 7-(diethylamino) coumarin-3-nitro group; preparing 7-(diethylamino) coumarin-3-amino group; preparation of the 7-(diethylamino) coumarin-3-hydroxyl group; adding 7-(diethylamino) coumarin-3-hydroxyl group and 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole into an acetonitrile solution, then adding potassium carbonate, carrying out a stirring reaction at room temperature, and after the reaction is finished, removing a solvent and purifying to obtain the novel fluorescent probe capable of quantitatively distinguishing and detecting Cys, Hcy and GSH at the same time. The invention develops a novel fluorescent probe, and the novel fluorescent probe can quantitatively distinguish and detect the contents of Cys, Hcy and GSH in blood plasma at the same time under the conditions of different pH values and different fluorescent channels.

The invention discloses a method for synthesizing a coumarin [4,3-d]pyrimidine derivative. The method comprises the following step: a 4-aminocoumarin compound, aromatic aldehyde and ammonium iodide react in chlorobenzene under the oxidizing conditions to obtain a target compound. The method of the invention has the advantages of simple steps, low cost, no metal participation, etc., can overcome the disadvantage in the prior art that a substrate needs to be highly pre-functionalized, the amount of the reaction reagent is excessive, the reactants are easy to absorb moisture, the reaction requires metal catalysis, the steps are cumbersome and it is difficult to directly prepare a pyrimidine ring all-carbon-substituted target product, and can play an important role in the research field of coumarinopyrimidine derivatives.