76results about How to "Quick Visual Inspection" patented technology

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and / or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The invention discloses a laser shear speckle interferometry defect detecting system based on image registration and fusion. The system comprises a laser light source, a beam expander, a beam splitter, a shearing device, a phase shifter, an image collector and a PC integrated with an image processing algorithm and a phase shifter control algorithm and is characterized in that double cameras and the image collector are utilized to simultaneously record the laser shear speckle interferometry stripe graphs of a to-be-detected object before and after heat loading and an original image, an image processing algorithm and a phase shifter control algorithm are used to process the laser shear speckle interferometry stripe graphs before and after the heat loading to obtain a phase difference image,and fusion and registration are performed on the phase difference image and the original image to fast and precisely positon defect detection positons.

The invention discloses a ratiometric fluorescent probe based on ion imprinting, preparation and application thereof. The fluorescent probe takes a red quantum dot as the core and is embedded in a silicon dioxide nanoparticle, then amination modification is performed on the surface of the silicon dioxide nanoparticle, and the amination modified surface is covalently coupled with a green quantum dot to obtain the ratiometric fluorescent probe based on ion imprinting. The ratiometric fluorescent probe detects metal ions based on ratiometric ion imprinting of quantum dots, has excellent specificity, good stability, simple preparation, cheap price and the like, greatly shortens the reaction time, reduces detection of cadmium ions to 25nM, and provides convenience for visualized quick detection.

The invention discloses a ratio fluorescent probe, a fluorescent paper chip and a detection method for detecting mercury ions, the ratio fluorescent probe comprises a background probe and a reaction probe, the background probe is composed of nitrogen-doped blue carbon quantum dots, the reaction probe is composed of red gold nanoclusters, the ratiometric fluorescent probe is formed by mixing red gold nanoclusters and nitrogen-doped blue carbon quantum dots, after mercury ions are added into the ratiometric fluorescent probe, the red fluorescence of the gold nanoclusters is gradually quenched, and the fluorescence of the nitrogen-doped blue carbon quantum dots is kept unchanged and is used as a reference signal; and therefore, the On-Off type fluorescent probe is formed. The two fluorescent materials of the ratiometric fluorescent probe are easy to prepare, and the ratiometric fluorescent probe has the characteristics of low toxicity, high fluorescence intensity, good biocompatibility and the like, has a wider color change range, and is more beneficial to detection of mercury ions. When the probe is fixed on a paper chip sensor, rapid visual detection of mercury ions can be realized.

Methods and a system for an electronic device to allow for indicating a size or scale of a rendering in a three dimensional view. A virtual scene is displayed on a display with a work product that is rendered, such as a document or packaging request having a job ticket. The rendering is provided with a common object that is a three dimensional virtual object that relates size to the document or packaging for approval in the proofing process. A quick visual cue is therefore provided to visually convey a realistic scale in proportion to the product rendered three dimensionally.

The invention discloses a Cpf1 kit for rapid detection of hereditary deafness pathogenic gene SLC26A4 mutation and a detection method thereof. The Cpf1 kit comprises a Cpf1 detection system. The Cpf1detection system comprises: a specific crRNA for SLC26A4, a Cpf1 protein and a single-stranded DNA reporting system. The specific crRNA is any one or more designed and synthesized for mutation sites.The single-stranded DNA reporting system comprises an ssDNA FQ reporter for fluorescence detection by an enzyme-labeled instrument and / or an ssDNA DB reporter for immune colloidal gold strip detection. By detecting SLC26A4 gene mutation sites with the Cpf1 for the first time, the kit of the invention has the advantages of high sensitivity, strong specificity, low time consumption, no dependence onlarge-scale experimental equipment and the like.

The invention discloses an insulin pen storage medical vehicle, which comprises a vehicle frame main body, a top-layer storage platform and rollers, wherein the top-layer storage platform comprises a top platform surface, and a first accommodation cabinet and a second accommodation cabinet, which are arranged below the top platform surface; the top platform is provided with an open groove communicated with the first accommodation cabinet; a circle of concave platform is arranged at a groove opening of the open groove in a downward sinking manner, and is detachably covered with a cover plate; multiple insulin pen placement plates are arranged in the first accommodation cabinet; multiple insulin pen clamping frames are arranged on the end face of at least one side of each insulin pen placement plate; a tag used for indicating patient information is adhered to each insulin pen clamping frame. The insulin pen storage medical vehicle has the effects that the insulin pen at a corresponding position can be taken to inject a corresponding patient according to the patient information on the tag A when the cover plate is removed, the insulin pens are placed in order, and can be rapidly taken, and medical staff can also push the insulin pen storage medical vehicle to each sickroom for centralized injection of patients, so that the insulin pen storage medical vehicle is high in practicability, and has good using effects.

The invention provides a fast visual detection method of organophosphorus type pesticide. Firstly, ZnTPyP is subjected to hydrothermal self-assembly by a cationic surface active agent for preparing nanometer porphyrin; then, the nanometer porphyrin is used for the fast visual detection on organophosphorus. On the basis of biomimetic peroxidase type activity of the nanometer porphyrin, 3,3',5,5'-tetramethyl benzidine is catalyzed and oxidized; the characteristic absorption in a 652nm position of the ultraviolet-visible spectrum occurs; colorless to blue chromogenic reaction is generated; specific reaction of the organophosphorus type pesticide and the nanometer porphyrin is utilized; the catalytic activity of the nanometer porphyrin is reduced; the generation of blue oxidation product oxTMBis reduced; the reduction of the absorbancy in the 652nm position of the ultraviolet-visible spectrum is shown; the reduction degree and the concentration of the organophosphorus type pesticide are in positive correlation relationship; the detection of the organophosphorus type pesticide is realized; the operation of the detection method is simple and convenient; the reagent toxicity is small; the cost is low; the sensitivity is high; the stability is high; the applicability is high.

The invention provides a colorimetric sensor for enhancing MOFs enzyme-like activity based on an aptamer as well as a preparation method and application of the colorimetric sensor, and belongs to the technical field of sensor preparation and fruit and vegetable quality detection. According to the present invention, the colorimetric sensor is constructed by using the metal organic framework material (MOFs) with peroxidase-like activity to catalyze and oxidize 3, 3', 5, 5'-Tetramethylbenzidine (TMB), and the colorimetric sensor can achieve the visual rapid monitoring of the pesticide residues in the fruits and the vegetables based on the enhancement effect of the aptamer on the catalytic activity of the MOFs and the specific recognition between the aptamer and the pesticide.

The invention discloses a method for visually detecting the concentration of hydrogen peroxide by using carbon quantum dots. By virtue of a linear relationship between the concentration of the hydrogen peroxide in a mixed solution and the absorbance of the mixed solution, the concentration of the hydrogen peroxide is detected; the mixed solution contains TMB, the carbon quantum dots, a phosphoricacid and citric acid buffer and a phosphate buffer. By the method, when the carbon quantum dots are used for visually detecting the concentration of the hydrogen peroxide, the detection process is simple and convenient, the sensitivity is high, the detection limit is low, and rapid visual detection of the hydrogen peroxide in an actual sample can be achieved; the detection method has low detectioncost, a great application value and a broad market prospect; in addition, a water-soluble carbon quantum dot solution prepared on the basis of porcine blood is good in stability, simple in preparation method, convenient to operate, low in cost, low in toxicity, environmentally friendly and pollution-free; the obtained quantum dots have good water solubility and can be directly used for detectingthe concentration of the hydrogen peroxide, so that the detection complexity and the detection cost are reduced.

The invention belongs to the field of environmental monitoring, and discloses an iron-based metal organic framework material Fe-MOFs and a synthesis method thereof. 3,5-dicarboxyphenylboronic acid is used as a ligand to be assembled with metal iron ions, and the iron-based metal organic framework material (Fe-MOFs) with boric acid functionalization is obtained under the action of a regulator. A research result shows that the Fe-MOFs nano material shows excellent peroxidase mimetic property. In the presence of hydrogen peroxide, the Fe-MOFs can catalytically oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to show a blue color. Meanwhile, when fluorine ions exist in the system, the peroxidase property of the Fe-MOFs is inhibited based on the specific recognition of boric acid on the fluorine ions and the strong interaction between metal ions and the fluorine ions. On the basis, the invention constructs a novel method for colorimetric detection of fluorine ions. The method has relatively high selectivity and sensitivity on detection of the fluorine ions, and has great application potential on visual detection of the fluorine ions in environmental monitoring.

The invention discloses a novel visual pathogen nucleic acid rapid detection chip which comprises a substrate and a flow path sheet. The substrate and the flow path sheet are sealed into one whole body; the substrate is composed of a bottom plate, a USB interface, a heating resistor and a thermosensitive element; the flow path sheet is a communicating pipeline structure composed of a buffer solution hole, a sample adding hole, a reaction region, a detection region and a waste liquid vessel; the detection region consists of a chromatography test paper card; a long-chain nucleic acid probe I, a long-chain gold-labeled nucleic acid probe II, a short-chain gold-labeled nucleic acid probe III and a short-chain gold-labeled nucleic acid probe IV are fixed on the chromatography test paper card; and the USB interface can be connected with a mobile phone charger and the like. According to the novel visual pathogen nucleic acid rapid detection chip, rapid visual detection of new coronavirus pathogens in a saliva sample is implemented, the saliva sample is taken, a lysis solution is added for lysis, RNA is extracted through a paramagnetic particle method, fluid is controlled through the liquid driving force of a buffer solution, the one-step reaction is performed, and visual detection of results is implemented within one hour without any instrument.

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for zinc ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system, (3)a HCR system, and (4) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of thetemplate can be realized in the presence of zinc ions, and an amplification product is allowed to form a G-quadruplex in a proper environment by triggering a HCR reaction; and color development is performed by utilizing the peroxidase-like activity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of zinc ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to zinc ions, and is more objective and accurate in detection results.

The invention relates to the technical field of plant pathogen detection, in particular to a loop-mediated isothermal amp lification (LAMP) primer for rapidly detecting meloidogyne javanica, application of the LAMP primer and a detection method of the LAMP primer. The LAMP primer comprises a forward outer primer Mj-F3, a reverse outer primer Mj-B3, a forward inner primer Mj-FIP and a reverse innerprimer Mj-BIP; and primer nucleotide sequences are sequentially shown as SEQ ID NO.2-5. A LAMP detection method is built based on the LAMP primer, can be applied to rapid detection and identificationof the meloidogyne javanica in nematode samples, plant root tissue samples and soil samples and has the advantages of high specificity, high sensitivity, simple instrument equipment, simple operation, fastness and high efficiency, visual determination of a result and the like, and high popularization and application value is achieved in aspects such as field rapid detection of the meloidogyne javanica and early diagnosis and guide scientific drug use of the meloidogyne javanica.

The invention relates to a light guide plate, a display device and a detection method for printing positioning of the light guide plate. A dimming figure is printed on the surface of the light guide plate and has set first offset tolerance, the surface of the light guide plate is provided with a first lateral side and a second lateral side opposite to the first lateral side, a first strip-shaped positioning reference figure and a second strip-shaped positioning reference figure are further printed on the surface of the light guide plate, the first positioning reference figure is parallel to the first lateral side, the second positioning reference figure is parallel to the second lateral side, and the sum of the distance between the inner lateral side of the first positioning reference figure and the first lateral side and the distance between the inner lateral side of the second positioning reference figure and the second lateral side is the double of the first offset tolerance. The dimming figure and the positioning reference figure are printed on the surface of the light guide plate together, whether printing positioning of the dimming figure meets the set offset tolerance or not can be simply judged by means of visual testing according to whether the positioning reference figures are imperfect or not, and the problem of difficulty in detecting printing positioning of the light guide plate is solved.

The invention provides a visual detection method of magnesium and zinc cutting type functional nucleic acids for universal partition and ultra-fast amplification. The visual detection method providedby the invention can perform ultra-fast amplification on templates the presence of magnesium and zinc ions by ingeniously designing primers, the templates and probes, enables amplification products toform a G-quadruplex in a suitable environment, further uses peroxidase-like activity of the G-quadruplex for color development, solves a problem that traditional PCR products are hard to be detectedvisually, and realizes fast and visual detection of magnesium and zinc ions. Moreover, the visual detection method provided by the invention has the characteristics of high specificity and high sensitivity to magnesium and zinc ions, and more objective and accurate detection results.

The invention discloses a visual detection method for aflatoxin B1. The visual detection method comprises the following steps: respectively linking sulfhydrylated aflatoxin B1 aptamer DNA1 and a complementary chain DNA2 of a sulfhydrylated aptamer to 13nm+ / -2nm nanogold, so to obtain an aptamer-nanogold probe and a complementary chain-nanogold probe, and carrying out detection by virtue of a competition method, wherein a drone is bonded with the aptamer when a to-be-detected object contains the drone, the aptamer-nanogold probe and the complementary chain-nanogold probe are in a free state, nanogold is easily gathered under a high salt concentration condition, the color of the nanogold is changed from red to blue, when the to-be-detected object does not contain the drone, the nanogold is not gathered under the high salt concentration condition, and the color of the nanogold is still red and is gradually changed from red to blue along the increase of the concentration of the drone; anddetecting an ultraviolet-visible spectrum of the solution at 200nm-800nm, so as to realize the quantitative detection of aflatoxin B1 in the to-be-detected object.

The invention relates to a rapid detection kit for pathogenic bacteria of phoma stem canker and a using method of the rapid detection kit. The rapid detection kit comprises a rapid detection method and a kit for leptosphaeria maculans and leptosphaeria biglobosa. The detection method for the pathogenic bacteria comprises specific amplification of target nucleic acid molecules and specific detection based on specific guide RNA, Cas12a and a nucleic acid probe. By using the method provided by the invention, whether the leptosphaeria maculans and the leptosphaeria biglobosa are contained in rape plant tissues can be rapidly detected. In combination with a nucleic acid isothermal amplification technology, the sensitivity of the detection method can be greatly improved, and the detection method can be used in fields and quarantine sites. The rapid detection kit for the pathogenic bacteria of the phoma stem canker is used for detecting the leptosphaeria maculans or leptosphaeria biglobosa, has the advantages of high sensitivity, good specificity, short consumed time, no dependence on large experimental equipment and the like, and is conveniently used for field disease investigation as well as rapid detection and identification diagnosis of the pathogenic bacteria of the phoma stem canker in primary laboratories.

The invention relates to technical field of biology and in particular to discloses a LAMP (Loop-Mediated Isothermal Amplification) primer group, a kit and a detection method for detecting lotus rot pathogen. A group of primer group F3 / B3 and FIP / BIP is designed by aiming at a lotus rot pathogen translation elongation factor (TEF-1 alpha) gene sequence, the primer group or the kit containing the primer group is used for detecting the lotus rot pathogen by virtue of LAMP. The LAMP primer group and the kit have the advantages of low cost, easiness in operation, simplicity, rapidness, visual detection result, judgment capability based on naked eyes, high sensitivity, strong specificity and the like, are applicable to lotus (lotus root) quarantine for introducing seeds, seedling and breeding lotus root quality test, lotus rot pathogen field early diagnosis and the like in basic departments, and have wide and practical application value.

The invention belongs to the field of protein detection, and relates to a microfluidic chip colorimetric detection method and kit for detecting creatine kinase isoenzyme. The method comprises the following steps: S1, preparing a linear polyacrylamide-DNA polymer; S2, synthesizing and modifying gold nanoparticles; S3, designing and assembling a micro-fluidic chip; S4, synthesizing DNA hydrogel; and S5, detecting. According to the invention, the DNA hydrogel is used for detecting the target object, the stability is good, the reaction specificity is high, and visual detection can be achieved; and the micro-fluidic chip is used for detection, and the purpose of portable quantitative detection is achieved.

The invention relates to a boron dipyrromethene fluorescent probe and a preparation method and application thereof in viscosity detection, and in particular relates to a method for measuring intracellular viscosity by using a fluorescent molecular probe based on a boron dipyrromethene structure. The fluorescence of the fluorescent probe is very weak in a low-viscosity system, the fluorescence of the fluorescent probe is enhanced in a high-viscosity solvent system such as glycerol, and meanwhile, a certain degree of emission spectrum red shift phenomenon is accompanied; therefore, the probe canbe used for detecting the viscosity in different solvent systems; and meanwhile, the probe has good biocompatibility and low toxicity, can be used for detecting viscosity change in cells, and has specific positioning performance on mitochondria.

The invention discloses a method for visually detecting phytoplasma based on CRISPR / Cas12a point cutting. According to the method, by designing crRNA aiming at a phytoplasma 16SrDNA sequence conserved region, the incision function of CRISPR / Cas12a protein is activated to cut linker-arm DNA so as to generate a required broken DNA fragment, and multiple phytoplasma are successfully detected by utilizing a nanogold color development technology, which has great significance for realizing rapid visual detection of phytoplasma in the field. The method disclosed by the invention has the characteristics of low cost, simplicity and convenience in operation, high efficiency, high sensitivity, high specificity and the like, naked eye judgment can be realized by color development, and the color development reaction effect is lasting and obvious. The dependence on large instruments and precision signal acquisition instruments in laboratories is reduced, and a new thought and a new choice are provided for detection of phytoplasma.

An underwater oil storage method, which includes the steps of: a) excavating a cellar in the ground earth and constructing a wall structure in the cellar to form a water pool, b) setting an oil tank in the water pool, c) supplying a predetermined amount of water to the water pool, and d) filling the oil to be stored into the oil tank and regulating the amount of water in the water pool subject to the amount of storage oil stored in the oil tank for enabling water in the water pool to support the oil tank and storage oil in the oil tank and to keep the oil tank in balance by means of the physical characteristic that the specific gravity of the storage oil is lower than water.

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system and (3) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can berealized in the presence of chromium ions, and an amplification product is allowed to form a G-quadruplex in a proper environment; and color development is performed by utilizing the peroxidase-likeactivity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of chromium ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to chromium ions, and is more objective and accurate in detection results.

The invention provides a sodium ion cutting type general partition ultrafast amplification visual sensor which comprises (1) a cutting system; (2) a sPCR (Polymerase Chain Reaction) amplication system; (3) an HCR system; (4) a detection system comprising an ABTS developing solution. According to the visual sensor disclosed by the invention, by ingeniously designing primers, templates and probes, the templates can be subjected to ultrafast amplification in the presence of sodium ions, and the amplification product enables the product to form G-quadruplex in a suitable environment by promoting the HCR reaction. Furthermore, developing is performed by utilizing mimetic peroxidase activity of the G-quadruplex, the problem that the traditional PCR product is difficult in visual detection is solved, and rapid and visual detection of the sodium ion is realized. Moreover, the sensor provided by the invention has the characteristics of high specificity and high sensitivity on the sodium ions, and the detection result is objective and accurate.